Mycoplasma hyopneumoniae Induces Grap, Gadd45, and secreted phosphoprotein 1 Gene Expression as Part of the Inflammatory Response in RAW264.7 Cells

황미현,최명진,박승춘 한국독성학회 2009 Toxicological Research Vol.25 No.3

Genes related to Mycoplasma hyopneumoniae-induced inflammation were identified using the genefishing technology, an improved method for identifying differentially expressed genes (DEGs) using an annealing control primer (ACP) system in RAW264.7 cells. After treatment with M. hyopneumoniae, 16 DEGs were expressed in RAW264.7 cells using a pre-screening system. Among these 16 DEGs, 11 DEGs (DEGs 1, 4, 5-10, 12-15) were selected and sequenced directly, revealing that DEG12 (Grap), DEG14 (Gadd45), and DEG15 (secreted phosphoprotein 1) were related to inflammatory cytokines. This is the first report that intact M. hyopneumoniae induces the expression of Grap, Gadd 45β, and secreted phosphoprotein 1 in RAW264.7 cells. Subsequently, these genes may be targets for screening novel inhibitors of the mycoplasmal inflammatory response.

기업의 M&A 전략 및 과세문제

황미현 서울시립대학교 도시과학대학 세무학과 2005 稅務學論集 Vol.18 No.2

기업의 M&A 전략 및 과세문제

인터넷 소비자정보사이트의 분류 및 기술적 수준 실태분석

황미현,이승신 대한가정학회 2002 Family and Environment Research Vol.40 No.5

This study was intended to make an analysis of the classification of Internet consumer information provided on the Internet and its realities with a focus on the realities of information characteristics and technological level to provide systematized consumer information for consumers. As a result, the following findings were obtained: First, it was found that information on consumer panel research, anti-site and consumerism is lacking in terms of the type of information currently provided in the Internet consumer information classification. Second, it was found that up-to-dateness, communicability and connectivity were high in the level of Internet consumer information characteristics. Third, it was found that publicity was relatively well carried out in terms of the technological level of Internet consumer information but that the realities of publicity was still lacking. Fourth, it is thought that it is necessary to construct the Internet consumer information provision site through several eye-level programs for elementary, middle and high school students in a systematic fashion in order to provide diverse consumers with Internet consumer information at several levels.

도라지 분말을 첨가한 스펀지케이크의 품질특성 및 라디칼 소거활성

황미현,김미리 한국식품조리과학회 2019 한국식품조리과학회지 Vol.35 No.3

Purpose: This study examined the antioxidant activities and physicochemical properties of sponge cake containing bellflower powder. Methods: The amounts of bellflower powder addition to sponge cake were 0%, 2.5%, 5.0%, and 7.5%. The moisture content, volume, specific gravity, sugar content, reducing sugar, pH, acidity, color, and texture were measured to assess the quality characteristics of sponge cake. The antioxidant activities were examined by measuring the total phenol content and DPPH radical scavenging activity. Results: The moisture contents increased with increasing amount of bellflower powder. The volume was lower in the sponge cake containing bellflower. The specific gravity was higher in the sponge cake with bellflower. The sugar content and reducing sugar contents decreased with increasing amount of bellflower powder. The pH of the sponge cake decreased with increasing amount of bellflower powder. As the amount of bellflower powder increased, the lightness (L) and yellowness (b) values of the sponge cake decreased. But the redness (a) increased. The textural properties by TPA showed that the hardness, gumminess, and chewiness increased with increasing amount of bellflower powder added. The antioxidant activities, including the total phenols and DPPH radical scavenging activities, increased with increasing addition of bellflower powder. In the preference test, sponge cake containing 5.0% received the highest score for overall preference. Conclusion: Based on these results, the antioxidant activities of the sponge cake containing bellflower powder were considered excellent and increased with increasing amount of bellflower powder.

2엽 수직축풍력발전기의 유동해석 및 실험 비교

황미현,김동현,이종욱,오민우,김명환,류경중 한국전산유체공학회 2010 한국전산유체공학회지 Vol.15 No.4

In this study, aerodynamic analysis have been conducted for 2-Bladed Vertical-Axis Wind Turbine (VAWT) configuration. Reynolds-averaged Navier-Stokes equations with Standard k-ω and SST k-ω turbulence models are solved for unsteady flow problems. Also, An Experiment model of 2-Bladed VAWT model has been designed in this study. Aerodynamic performance test of the present VAWT model experiments are effectively conducted using the vehicle testing system. The comparison result between the experiment and the Computational Fluid Dynamics (CFD) analysis are presented In order to verify the accuracy of the different turbulent model.

아동의 부모 사별경험 유무에 따른 동작성 가족화(K-F-D)검사의 인물상의 특징, 활동내용, 스타일 반응연구

황미현,장연집 한국미술치료학회 1999 美術治療硏究 Vol.6 No.2

사별이란 죽음으로 인해 사랑하는 사람을 상실하는 것으로 아동의 성장발달에 중요한 1차적 환경이 되는 가족성원 중 특히 부모와의 사별은 아동에게 있어 정서적 · 사회적 · 경제적 지지를 해주는 한 개인의 일생에 있어 가정 중요한 사회적 지지원을 상실하는경험이다(Guthrie외, 1997). 또한 아동기는 발달수준이 아직 미성숙하고 죽음에 대한 대처능력이 불충분하기 때문에, 이 시기는 사별로 인해 겪게 되는 여려 가지 고통에 보다 취약하다고 할 수 있다(Osterweis, 1984). 부모의 죽음은 사별당한 아동에게 다양한 측면의 사별반응을 유발시킬뿐만 아니라, 이러 사별경험은 아동기 후기 · 청소년기 · 성인기 걸쳐 장기적이고 지속적으로 부정적인 영향을 미치게 된다. 또한 이러한 죽음은 아동뿐만 아니라 생존부모, 형제 · 자매를 비롯하여 그들과의 관계, 가정의 전체적인 분위기 그리고 가족구성원들간의 상호작용 등과 같은 가족역동에도 영향을 미치고 있다. 따라서 사별을 경험한 아동들의 심리적 특성을 이해하고 그들을 보다 효과적으로 지지하기 위해서느 사별이후 변화된 가족역동, 가족성원과 관련된 그들의 감정 및 인식에 대한 이해가 선행되어야 한다. The purpose of this Study was (1) to examine bereaved children' s response, (2) to design psychological education program which support bereaved children. The subject for this study were 7 intact children and 70 bereaved children attending elementary schools. The instrument used in this study was Kinetic Family Drawing Test, the K-F-D questionnaire and 22 variables K-F-D scoring manual. Statistical method adopted for data analysis was chi square test. The result of this study were as follow : (1) There was a statistically significant difference between intact children's "Figure Characteristic If response and bereaγed children’s. The younger grade bereaved children obtainεd higher maladjustment score in shading and oblique line, evasion, number of family, and the location of self. Male grade bereaved children obtained higher maladjustment score in arm extension, shading and oblique line, evasion, number of family, and the location of self. (2) There was a statistically significant difference between intact children's "Action" response and bereaved children’s. The younger grade bereaved children obtained higher maladjustment score in baniers, activity level of mother, activity level of self, interaction of figure. interaction with self. Male grade bereaved children obtained rugher maladjustment score in baniers, activity level of mother, activity level of self, interaction of figures, interaction with self. (3) There was a statistically significant difference between intact children's "Style" response and bereaved children's The younger grade bereaved children obtained higher maladjustment score in underlining variables and encapsulation. Male grade bereaved children obtained higher maladjusunent score in underlining variables and encapsulation. This result suggested that K-F-D are useful to understand the emotional conflicts and problem felt from family members in bereaved children. Further research is necessary to examine other variables of bereavement s

GLUT-1 Expression in Preoperative Endoscopic Biopsy for LN Metastasis on PET/CT in Colorectal Cancer

황미현,한정호,김홍식 대한내과학회 2023 대한내과학회 추계학술대회 Vol.2023 No.2

늦어도 괜찮아, 천천히

황미현 천년의시작 2019 (계간)시작 Vol.18 No.1

송년모임을 위한 특별 식단

황미현 대한영양사회 2000 國民營養 Vol.224 No.-

[신작시] 떨어진 별 외 2편

황미현 천년의시작 2019 (계간)시작 Vol.18 No.4