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        Immunoassay 를 이용한 Steroids 및 생약성분의 미량분석

        최명자 한국생약학회 1987 생약학회지 Vol.18 No.1

        Specificity가 큰 미량분석에 초점을 둔 Immunoassay는 hormone, tumor antigen, drug, virus등 여러분야에 걸쳐 보편화되고 있다. 그중 특히 antigenicity가 적은 hapten group이 주성분을 이루는 natural alkaloid나 glycoside등 drug assay에는 large molecule의 protein을 conjugate하여 specific antibody를 얻고 있다. 본 실험실에서는 이번 제10회 Asian game 수상자들의 약물 복용 여부를 판단하기 위한 일부 조치로서 행한 Radioimmunoassay와 Fluorescent polarization immunoassay의 performance characteristics를 연구하였으며 standard curve의 재현성, sensitivity, crossreactivity, precision등을 규명하였다. 이 anabolic steroid RIA의 standard curve range는 50pg/㎖서 10ng/㎖로서, 이를 이용하여 운동선수들의 뇨중 testosterone, epitestosterone 농도 및 T/E ratio의 정상치 및 분포도를 확립하였다. 또한 17α-methyl testosterone, 19-nor-testosterone등 specific structure 별로 분석한 RIA의 분석결과를 GC/MSD 결과와 비교하여 좋은 상관관계를 얻었다. Alkaloid narcotics인 opiates, cocaine, phoney-clidine은 homogeneous immunoassay인 FPIA로 분석했으며 이를 이용하여 571명의 선수들 뇨를 분석한 결과 모두 음성으로 positive/negative의 분계농도인 threshold의 10% 미만이었다. 이러한 Immunoassay 분석법은 specific atibody를 생산하는 데 따라 개발할 수 있는 sensitive assay method로서 적은 volume의 sample을 가지고 미량분석을 할 수 있으므로 natural alkaloid 및 synthetic drug assay에 많이 이용되고 있다.

      • 一部 國民學校 學生들의 寄生충豫防處에 關한 家庭과 學校에서의 知識 및 習慣에 對한 調査 : 公·私立 國民學校 5學年 學生을 對象으로

        崔明子 中央醫學社 1975 中央醫學 Vol.28 No.5

        This survey study was conducted to find out knowledge and hygienic habits about the prevention of parasitic infection in 432 public school and 250 private school children who were enrolled at the fifth grade during the period of February 18-22, 1975. The results of this study were summarized as follows: 1) On educational background of the mothers, 25.5% Were graduates of college and over. The proportion between public and private school of the mother's educational background were 14.9% to 85.1%. 2) Among surveyed students, 84.9% of them answered correctly on question of name of Ascaris lumbricoides (round warm) and 33.6% in case of clonorchis sinensis. 3) Correct response of knowledge test on symptoms and parasitic area of Ascaris lumbricoides were as follows. a. "Vomiting and frequent pain in abdomen" -60.2% was correct answer in public school and 84.4% at the private school. b. "Parasite at small intestine"-63.2% was correct answer in public school and 78.4% at the private school. c. "Enter through mouth "-60.9% was correct answer in public school and 92.0% at the private school. 4) Correct answer was low in question of prevention method on I'aragonimus westermani (lung hook) was as low as 44.0%. 5) The question on suitable reservoir for a parasite were correctly answered in 62.8% of the student to the item of human feces and contaminated soil and. the item of room dust was 1106.4, 05. 6) The question on safe washing method of vegetables was answered in 59.7% of the students in item "wash several times in running water". 7) The question on taking time for antiparasitic drug is whenever eggs are found in stool examination was correctly answered in 50.6% of the students. 8) The "yes" in question of whether did they have chance to learn about. parasite control at the school was 89.1% and 31. 6% of them responded it was athletic class. 9) The question on by whom did they learned was 90.5% answered they were taught by the class-room teacher, and 9.5% said by the school nurse. 10) The preferred foods of the students were mentioned as kimchi and Sang. chussam (fresh vegetables to eat) in 83.2% of the students. 11) No relationship between parasitic infection episode and age of mothers and birth order of students were found as a result of statistical test (chi-square test p>O.05). 12) Significant relationship between parasite infection history of the students and educational level of mothers; the lower the education of mother, the more times infected by the parasite warms (p <0.05).

      • 一部 男性의 飮酒行爲와 家庭에 미치는 影響에 對한 調査

        崔明子 서울大學校保健大學院 1975 公衆保健雜誌 Vol.12 No.2

        This survey study on drinking behaviors of men and its influences to their families was conducted by means of the questionnaire toward 489 employees at seven industrial companies in Seoul during a period of August, 1975. The results of the study were appeared as follows: 1. The degrees of drinking were classified into four categories according to the Miller and Burt's Method. Out of the 489 respondents, the proportions of drinking degrees appeared as non-drinkers 8.4%, small drinkers 49.1%, medium drinkers 29.4%, and heavy drinkers 13.1%. 2. No relationships between the age and/or occupation and the degree of drinking behaviors were observed. 3. The proportions of non-drinkers and heavy drinkers among those with educational level of middle school or less were 11.9% each, while the proportions of the college graduates were 6.1% and 15.5%, respectively. 4. The proportions of non-drinkers and small drinkers in the group with monthly income of less than 50,000 Won were 73.8% and 26.2%, while the proportions in the group with monthly income of more than 100,000 Won were 53.2% and 46.8%, respectively. 5. The proportions of non-and small drinkers and medium and heavy drinkers among those who hame no family members of school-age children yet were 50.0% each, while the proportions among those who have more than four school-age children were 71.1% and 28.9%, respectively. 6. Of the drinking motives of the heavy drinkers, it was more or less habitual, but that of the small drinkers was for social gathering or business. 7. The distribution of most popular places of drinking were appeared as Daipojip 65.4% and beer hall 16.5%. Their most favorable kinds of liquor was the soju with the proportion of 57.4% and next was beer with 25.4%. 8. In number of companions in drinking, one or two were the most frequent one in small drinkers, and more than three were the most frequent number of companions in medium and heavy drinkers. 9. The severer the degree of drinking, the more frequent gastric ulcer, hang over in next morning, gastric distress and absence to work in next day were observed. 10. In the frequency of very often quarrel with their family members, it was 6.3% in the group of small drinkers, and was 20.3% in the group of heavy drinkers. 11. There were no significant relationships between the degree of drinking and the degree of economic problems of the family, conflicts with wife, disharmony of family life, and the problems in child rearing.

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