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포황 메탄올 추출물에 의한 인체 대장암 세포주 HT29의 G2/M Arrest 및 Apoptosis 유발
진수정 ( Jin Soo Jung ),윤승근 ( Seung Geun Yun ),오유나 ( You Na Oh ),이지영 ( Ji Young Lee ),박현진 ( Hyun Jin Park ),진경숙 ( Kyong Suk Jin ),권현주 ( Hyun Ju Kwon ),김병우 ( Byung Woo Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2013 한국미생물·생명공학회지 Vol.41 No.4
본 연구에서는 인체 대장암세포인 HT29를 사용하여 포황 메탄올 추출물(Methanol extract of Typha orientalis, METO)의 항암 활성 및 그 분자적 기전에 관하여 분석하였다. 먼저 METO가 다양한 암세포의 증식에 미치는 영향을 분석한 결과, 인체 대장암 세포, 폐암 세포, 간암 세포 등의 세포증식을 억제하였으며 그 중에서도 대장암 세포인 HT29에 대해 강한 세포 증식 억제 효과를 나타내었다. METO에 의한 세포 증식 억제 기전을 분석하기 위하여 Flow cytometry analysis를 수행한 결과, METO 농도의존적으로 HT29 세포의 G2/M기 세포분포가 증가하고 아울러 apoptosis 유발군인 SubG1기 세포분포가 증가하는 것을 확인할 수 있었다. METO에 의한 HT29 세포의 G2/M arrest는 Cdc2의 inactive form인 phospho-Cdc2의 증가에 의한 G2/M checkpoint 관련 단백질의 활성억제에 의한 것이라 사료된다. 이러한 phospho-Cdc2의 증가는 METO에 의해 발현이 증가된 Wee1 kinase와 발현이 감소된 Cdc25C phosphatase에 의해 야기된 것임을 확인하였다. 또한 METO에 의한 HT29의 apoptosis 유도에 관한 분자적 기전 분석을 위해 Western blot analysis를 수행한 결과, METO 농도가 증가할수록 종양 억제 유전자인 p53, death receptor인 FAS, Bcl-2 family 중 pro-apoptotic 단백질인 Bax 및 cytosolic cytochrome C의 발현이 증가되고, Caspase-3가 활성화되어 단편화된 Caspase-3의 증가가 관찰되었다. 또한 활성화된 Caspase-3의 기질 단백질인 PARP의 단편화가 일어나 apoptosis가 유도되는 것을 알 수 있었다. 이상의 결과들로부터 METO는 인체 대장암세포 HT29의 G2/M arrest 및 apoptosis 유도에 의한 항암활성을 보유함을 확인하였다. Typha orientalis, also known as bulrush or cattail, is a perennial herbaceous plant found in freshwater wetlands and has been widely used in constructed wetlands for wastewater treatment. Recent data has revealed that SH21B, a mixture composed of seven herbs including T. orientalis, exhibited an anti-adipogenic activity by the inhibition of the expression of adipogenic regulators. However, the anti-cancer effect of T. orientalis and its molecular mechanisms remain unclear. In this study, we evaluated the anti-cancer effect and its mechanism in the methanol extract of T. orientalis (METO) on human colon carcinoma HT29 cells. It was found that METO treatment showed cytotoxic activity in a dose-dependent manner, and induced G2/M cell cycle arrest and apoptosis in HT29 cells. The induction of G2/M arrest by METO was associated with the up-regulation of phospho-Cdc2 (Tyr15), an inactive form of Cdc2 and the down-regulation of Cdc25c phosphatase. METO also induced tumor suppressor p53 and cyclin-dependent kinase inhibitor p21 (WAF1/CIP1) expression. In addition, METO-induced apoptosis was characterized by the proteolytic activation of caspase-3, degradation of poly ADP ribose polymerase (PARP), and up-regulation of death receptor FAS and pro-apoptotic Bax expression. Collectively, these results indicate that the cell cycle inhibition and apoptosis induction of METO in HT29 cells allows for the possibility of its use in anti-cancer therapies.
산초 추출물 및 분획물의 항산화, 미백 및 항염증 활성
진경숙 ( Kyong Suk Jin ),오유나 ( You Na Oh ),박정애 ( Jung Ae Park ),이지영 ( Ji Young Lee ),진수정 ( Soo Jung Jin ),현숙경 ( Sook Kyung Hyun ),황혜진 ( Hye Jin Hwang ),권현주 ( Hyun Ju Kwon ),김병우 ( Byung Woo Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2012 한국미생물·생명공학회지 Vol.40 No.4
본 연구에서는 한약재로 사용되고 있는 산초 과피 추출물의 기능성 소재로서의 활용 가능성을 알아보기 위해 산초 추출물 및 분획물이 보유한 항산화, 미백 및 항염증 활성을 in vitro assay system 및 cell culture model system을 이용하여 분석하였다. 그 결과 산초 MeOH, EtOH, 열수 추출물 모두 강한 DPPH radical 소거 활성 및 tyrosinase 효소 활성저해를 통한 DOPA 산화 억제능을 보였다. B16F10 mouse melanoma cell을 이용하여 α-MSH에 의해 유도된 세포 내 melanin 생성에 미치는 산초 추출물의 영향을 알아본 결과 농도의존적인 melanin 생성 억제능을 보였고 세포의 tyrosinase 활성 저해능 또한 이와 일치하는 결과를 보여 산초 추출물에 의한 melanin 생성 억제능이 tyrosinase 효소 활성 억제 및 melanogenesis 관련 단백질 발현 저해에서 기인한 것으로 판단된다. 또한 RAW 264.7 murine macrophage cell을 이용하여 산초 추출물의 항염증 활성을 분석한 결과 농도의존적인 NO 생성 저해능을 보였으며 이는 NO 생성 단백질인 iNOS의 발현 저해에서 기인함을 단백질 발현 분석을 통해 확인하였다. 산초 추출물에 존재하는 활성물질을 규명하기 위해 용매 분획을 실시한 후 각 분획물의 미백 및 항염증 활성을 비교 분석한 결과 물 층을 제외한 모든 용매분획이 활성을 보유함을 확인하였다. 이러한 결과를 통해 산초 추출물이 높은 항산화능과 미백활성, 그리고 항염증 활성을 보유함을 확인할 수 있었으며, 이러한 결과는 미백활성을 비롯한 생리활성 보유 천연물 소재 탐구의 기초자료로 유용하게 쓰일 것으로 사료된다. This study was designed to explore new nutraceutical and cosmetic resources possessing biological activities from the plant kingdom. To fulfill this purpose, we analyzed the anti-oxidative, anti-melanogenic, and anti-inflammatory activities of Zanthoxylum schinifolium extract (ZSE) and its solvent fractions using in vitro assays and cell culture model systems. Three kinds of ZSE treated with methanol, ethanol, and water exhibited potent anti-oxidative activities through DPPH radical scavenging capacity, and inhibited in vitro DOPA oxidation. Furthermore, Z. schinifolium methanol extract (ZSME) inhibited the α-melanocyte stimulating hormone, which induces melanin contents in B16F10 cells. Its anti-melanogenic activity originates from the inhibition of tyrosinase enzyme activity and melanogenesis related protein expression. Moreover, lipopolysaccharide induced nitric oxide production in the RAW 264.7 cell line was also ameliorated by ZSME treatment in a dose dependent manner. Among the four solvent fractions of ZSME treated with dichloromethane, ethyl acetate, nbutanol, and water, three fractions, except water, showed significant anti-melanogenic and anti-inflammatory activities. Taken together, these results provide important new insights into Z. schinifolium, indicating that it possesses numerous biological activities such as anti-oxidative, anti-melanogenic, and anti-inflammatory activities. Therefore, it may well serve as a promising material in the field of nutraceuticals and cosmetics.
재발성 직장암의 간전이 환자에서 시행된 전간 혈류 차단과 정맥 혈류 우회술 그리고 장기 보존액 주입 하의 ante situm 간절제술
주종우(Chong Woo Chu),김형철(Hyung Chul Kim),신응진(Eung Jin Shin),임철환(Cheol Wan Lim),조규석(Gyu Seok Cho),정준철(Jun Cheol Chung),정귀애(Gui Ae Jeong),송옥평(Ok Pyung Song),진수지(Soo Ji Jin),김희경(Hee Kyung Kim),박성진(Seong Ji 한국간담췌외과학회 2008 한국간담췌외과학회지 Vol.12 No.2
We present here a case of recurrent rectal cancer liver metastasis that was managed with ante situm liver resection under total vascular exclusion (TVE) and venovenous bypass with hypothermic perfusion. A 58-year-old man who suffered with rectal cancer liver metastasis was transferred to our hospital in January 2006. A left lateral sectionectomy had been previously performed. Recurrent lesion developed in segments I, IV and VIII one year after the first hepatectomy. The tumor was 5 cm in diameter and it involved the confluence of the hepatic veins and the retrohepatic vena cava. An incomplete tumor-free margin and massive bleeding were expected with performing a conventional liver resection, together with vena cava reconstruction. Therefore, we planned an ante situm liver resection under TVE and venovenous bypass with hypothermic perfusion. After adhesiolysis, hilar dissection was carried out. The inflow to the medial segment was interrupted, and then the liver and inferior vena cava (IVC) were mobilized fully. During controlling the bleeding of a short hepatic vein, we found adhesion of the hepatocaval portion. Therefore, TVE and venovenous bypass were performed along with suprahepatic IVC transection. The long conduit of V5 was preserved during hepatic parenchymal dissection, and the paracaval portion of the caudate lobe was readily detached from the IVC. The suprahepatic IVC was reconstructed after V5 reconstruction with using the saphenous vein. Portal vein anastomosis was then conducted. After reperfusion, an end-to-side anastomosis was performed between the saphenous vein graft and the IVC. Finally, a Roux-en- Y hepaticojejunostomy was carried out. The patient remains well without recurrence 12 months after the last operation.
절제 불가능한 원발성 간세포암종에 대하여 DC beads(r)를 사용한 반복적인 경동맥 화학색전술 후 간절제술을 시행한 1예
송정엽 ( Jeong-yeop Song ),김영석 ( Young Seok Kim ),이재명 ( Jae Myeong Lee ),진수지 ( Soo Ji Jin ),최규성 ( Kyu Sung Choi,),이윤나 ( Yun Nah Lee ),김상균 ( Sang Gyune Kim ),정승원 ( Sung Won Jeong ),장재영 ( Jae Young Jang ) 대한간암학회 2013 대한간암학회지 Vol.13 No.1
In patients with unresectable hepatocellular carcinoma (HCC) and no anti-cancer treatment, the prognosis is quite poor. But in some cases, repeated sessions of transcatheter arterial chemoembolization (TACE) reduce the tumor size even to resectable, and post-TACE resection may prolong the survival time. We present a case of 50-year-old HBV carrier woman with abdominal distension. The diagnosis was huge HCC with intrahepatic metastasis. Repeated intra-arterial injections of adriamycin mixed lipiodol or DC beads(R) (100-300/300-500/500-700 μm, ⓒBIOCOMPATIBLES UK LTD) were instituted through ten sessions for 13 months. The tumor size became reduced with a partial response after 10th TACE and post-TACE resection was performed. No visible HCCs and decreased tumor markers were noted on the examinations 3 months after the resection.
박진수(Park, Jin-Soo),백지숙(Baek, Ji-Suk),양태현(Yang, Tae-Hyun),김창수(Kim, Chang-Soo),임성대(Yim, Sung-Dae),박구곤(Park, Gu-Gon) 한국신재생에너지학회 2009 한국신재생에너지학회 학술대회논문집 Vol.2009 No.06
This study presents preparation and characterization of composite membranes based on ionic liquids. The ionic liquids act as water in sulfonated membranes. On the behalf of ionic conduction through ionic liquid inside the membranes, non-aqueous membranes showed Arrenhius dependence on temperature with no external humidification. It was implied that hopping mechanism of proton was dominant in the ionic liquid based membranes. In addition, small angle X-ray (SAXS) studies provided the information on morphology of ionic clusters formed by the interaction between sulfonic acid groups of the polymers and ionic liquids. The SAXS spectra showed matrix peaks, ionomer peaks and Prodo's law for Nafion based composite membranes and only matrix peaks for hydrocarbon based ones. However, ionic conductivity and atomic force microscopy (AFM) images showed the clear formation of ionic clusters of the hydrocarbon based composite membranes. It implies for ionic liquid based high temperature membranes that it is important to use sulfonated polymers as solid matrix of ionic liquid which can form clear ionic clusters in SAXS spectra.
홍지영 ( Ji Young Hong ),이상국 ( Sang Kook Lee ),조용진 ( Yong Jin Cho ),김철진 ( Chul Jin Kim ),김남수 ( Nam Soo Kim ),김종태 ( Chong Tai Kim ),맹진수 ( Jin Soo Maeng ) 한국산업식품공학회 2012 산업 식품공학 Vol.16 No.1
Strawberries have various fruit phenolics which provide biological activities such as antioxidant, anti-inflammatory, and anti-proliferative potential against various cancer cells. Previous studies showed that such phenolics might be enriched by ultraviolet irradiation (UV) after harvesting fruits and vegetables. In the present study, we investigated the effect of post-harvest ultraviolet irradiation on the biological activities of strawberries. When 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, induction of quinone reductase activity, inhibitory effect on the proliferation of various human cancer cells, and inhibitory effect on the expression of inducible nitric oxide synthase (iNOS) of UV-irradiated strawberries using Maehyang, Akihime, and Red pearl, were evaluated, the biological activities were, in general, enhanced by ultraviolet irradiation after harvest.
박진수 ( Jin Soo Park ),김지혜 ( Ji Hye Kim ),윤문경 ( Moon Gyung Yoon ),김정은 ( Jung Eun Kim ),신유섭 ( Yoo Seob Shin ) 대한천식알레르기학회(구 대한알레르기학회) 2016 Allergy Asthma & Respiratory Disease Vol.4 No.1
Seafood is one of the common causes of food allergies to adults. The sea hare Aplysia kurodai is a marine mollusk which belongs to invertebrate gastropod that has been consumed as a food in Korea. Cases of acute toxic hepatitis after ingestion of sea hares have been reported, but few cases of allergic reactions to sea hare have been reported in the literature. A 33-year-old man was referred to our Emergency Department due to urticaria and periorbital/perioral swelling after eating sea hares. Approximately 10 years ago, he experienced similar allergic reactions to it. Skin prick and intradermal tests showed strong positive responses to crude sea hare allergen extract. He was diagnosed with food allergy to sea hares. We herein report the first case of sea hare allergy after ingestion.
박진수,이용범,유정한,박용욱,노규철,정국진,김홍균,황지효,김형년,송영석,손원수,Park, Jin-Soo,Lee, Yong-Beom,Yoo, Jung-Han,Park, Yong-Wook,Rowe, Kyu-Cheol,Chung, Kook-Jin,Kim, Hong-Kyun,Hwang, Ji-Hyo,Kim, Hyung-Nyeon,Song, Young-Suk,Son 대한미세수술학회 2010 Archives of reconstructive microsurgery Vol.19 No.1
Interpostion arthroplasty with allograft has been known as a useful option for the shoulder arthropathy, but it has a limitation to immune response. We performed the pectoralis major muscle transfer for shoulder arthropathy. From January 2007 to December 2007, we performed the pectoralis major muscle transfer in 2 patients. They were 1 man and 1 woman, and the mean age of the patients was 70 years (range, 65 to 75). The average follow-up after surgery was 13 months (range, 12 to 14). We analyzed the clinical results by the American Shoulder and Elbow Surgeons (ASES) Score, and evaluated the pain by Visual Analogue Scale. The level of pain reduced from a preoperative average of 10 to a postoperative average of 1. The ASES scores increased from a preoperative mean of 9 to a postoperative mean of 58. These results indicated that interposition arthroplasty with the pectoralis major muscle transfer is a reliable treatment method for the shoulder arthropathy improving shoulder pain, and patient satisfaction.