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Cancer immunotherapy with hMSC based modulator
진민영,김경섭,이주영,한지은,황희숙,이종환,나건 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1
Photodynamic therapy (PDT) which generates reactive oxygen species (ROS) has been attracted attention because of ability to kill cancer cells with high therapeutic efficacy. Recently, immunotherapies with nano-based technology have been used to enhance the PDT effect. However, nanotechnology combinated immunotherapies have limitations, short half-life and serious side effects such as normal cell toxicity. To alleviate these problems, we designed the immunotherapy strategy that uses the human mesenchymal stem cell (hMSC) augment polymer and photosensitizer (PS) (hMSC-PP). Due to this strategy, hMSC-PPs can migrate to the lesion sites and avoid the immune system itself. In vivo test, under the laser irradiation to the hMSC-PPs, immune cells are increased at tumor lesion, because pro-inflammatory cytokine levels were increased. Resultingly, tumor growth was inhibited. As a result, hMSC-PP proved their significant potential for the cancer immunotherapy.
Tumor diagnosis with gadolinium - based nanoparticles
진민영,김경섭,박우람,나건 한국공업화학회 2020 한국공업화학회 연구논문 초록집 Vol.2020 No.-
Last several years, Gadolinium (Gd) - based contrast agent has been verified their ability as a T1 contrast agent. In addition, stem cells which have homing effects to tumor sites have been used for diagnosis and cure the tumor. However, Gd contrast agents have low cellular internalization, hydrophilic properties. In order to solve the limitations, we added photosensitizer (PS) to linear chelator conjugated Gd-based nanoparticles (PS-LCGN) through Photochemical internalization (PCI) to improve cellular internalization. Using this method, we entrapped the PS-LCGN into the human mesenchymal stem cells (hMSCs). Measuring the PS-LCGN with MRI scanner, PS-LCGN exhibited higher intensity than Gd - linear chelator. Under laser, PS damaged the cell membrane and enhanced internalization efficiency without side effects. In vivo test, using optical imaging and MRI scanner, we observed PS-LCGN enabled to detect the tumor. Consequently, PS-LCGN proved their potentials to use in tumor diagnosis.
ROS producible Light-dependent micelle for cancer treatment by using endo/lysosome escape
진민영,김기홍,이충성,나건 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0
Last several years, technology with nano scale micelles has been drawn huge attention about disease treatment and diagnosis especially in cancer. In addition, among the stimuli, Reactive Oxygen Species (ROS) can control biological functions. On the oher hand, ROS could cause side effects or reduce therapeutic effect. Moreover, since the endosome and lysosome are known as the barriers, to escape endosome and lysosome would be important strategy.To solve the abovementioned problems, we fabricated light-dependent ROS producible micelle (LDRM) with photosensitizer(PS) that includes drugs. ROS were produced by PS at certain wavelength.After that, LDRM was changed their properties and facilitate drug release. At the same time, residual ROS at the cancer site assist drug to avoid endosome and lysosome. In vivo test, LDRM with irradiation presents therapeutic effects that reducing tumor size. As a result, LDRM shows enormous potential at therapeutic effect especially cancer and drug release.
Endosome and lysosome evading ROS generable light reactive micelle for cancer treatment
진민영,김기홍,이충성,나건 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1
Over the years, the nanotechnology with micelles has been proven their potential to diagnosis and alleviate the cancers. Furthermore, reactive oxygen species (ROS) which is known for their ability to regulate biological functions. In contrast, their limitations such as drug release profile and severe side effects have been become a challenge to use. Additionally, evading the endosome and lysosome that are kind of the biological barricades would be significant approach to be a suitable treatment. In order to resolve the aforementioned challenges, we constructed drug and photosensitizer (PS) loaded ROS generable light reactive micelle (RLRM). Under the specific wavelength, PS generate ROS. Consequently, ROS make RLRM to release drugs, and the layer of endosome and lysosome become weaken in the tumor. In vivo test, under the irradiation, RLRM showed tend to reduce the tumor size. In summary, RLRM successfully released drug and PS, thus it could be led the field of cancer treatment.
ROS producible hMSC complex for Tumor immunotherapy
진민영,김경섭,이주영,한지은,황희숙,이종환,나건 한국공업화학회 2020 한국공업화학회 연구논문 초록집 Vol.2020 No.-
Last several years, Photodynamic therapy (PDT) has been verified their capability to trigger the immune system for killing cancer cells, since it is able to produce reactive oxygen species (ROS). Furthermore, to enhance the therapeutic efficacy of PDT, nanotechnology-based immunotherapy has been studied with PDT. However, there are several problems such as toxicity and their short half-life still remained. To resolve the limitations, we developed the immunotherapy with human mesenchymal stem cell (hMSC) complex which is composed of photosensitizers (PS) and polymers (hMPS). This complex can migrate to the lesion site, produce reactive oxygen species (ROS) and secretes cytokines without side effects of immune system. In vivo, we observed hMPS can recruit the immune cells since it secreted pro-inflammatory cytokines. Also, hMPS prohibited the tumor growth in the mouse model. Consequently, hMPS showed their potentials to be the platform for cancer immunotherapy.
Biocompatible carbonized iodine-doped dots for contrast-enhanced CT imaging
Yohan Jeong,진민영,김경섭,나건 한국생체재료학회 2022 생체재료학회지 Vol.26 No.3
Background: Computed tomography (CT) imaging has been widely used for the diagnosis and surveillance of diseases. Although CT is attracting attention due to its reasonable price, short scan time, and excellent diagnostic ability, there are severe drawbacks of conventional CT contrast agents, such as low sensitivity, serious toxicity, and complicated synthesis process. Herein, we describe iodine-doped carbon dots (IDC) for enhancing the abilities of CT contrast agents. Method: IDC was synthesized by one-pot hydrothermal synthesis for 4 h at 180 ᕑ and analysis of its structure and size distribution with UV–Vis, XPS, FT-IR, NMR, TEM, and DLS. Furthermore, the CT values of IDC were calculated and compared with those of conventional CT contrast agents (Iohexol), and the in vitro and in vivo toxicities of IDC were determined to prove their safety. Results: IDC showed improved CT contrast enhancement compared to iohexol. The biocompatibility of the IDC was verified via cytotoxicity tests, hemolysis assays, chemical analysis, and histological analysis. The osmotic pressure of IDC was lower than that of iohexol, resulting in no dilution-induced contrast decrease in plasma. Conclusion: Based on these results, the remarkable CT contrast enhancement and biocompatibility of IDC can be used as an effective CT contrast agent for the diagnosis of various diseases compared with conventional CT contrast agents.