Tocolytic effect of Clostridium botulinum toxin A on mifepristone-induced preterm Labor in rats

장항용,이경미,송준영,문명진,한원보,장성운,조정현,안은희 대한산부인과학회 2011 대한산부인과학회 학술대회 Vol.97 No.-

Effects of Autologous Platelet-Rich Plasma on Regeneration of Damaged Endometrium in Female Rats

장항용,김용민,명수민,최정민,김탁,천용필,박현태 연세대학교의과대학 2017 Yonsei medical journal Vol.58 No.6

Purpose: To investigate whether autologous platelet-rich plasma (PRP) treatment can improve regeneration of the endometrium in an experimental model of ethanol-induced damage. Materials and Methods: Sixty female Sprague-Dawley rats were randomly assigned into three groups: control group, ethanol group, and PRP-treated group (administration of 0.25 mL of PRP into both uterine cavities 72 hours after ethanol injection). After 15 days of endometrial damage, all the animals were sacrificed during the estrous cycle, and samples were taken from the mid-uterine horn. Functional and structural recovery of the endometrium was analyzed by hematoxylin-eosin (H&E) and Masson trichrome(MT) staining, real-time polymerase chain reaction (PCR) assay, and immuno-histochemical (IHC) analyses. Results: H&E and MT staining confirmed significantly decreased fibrosis and increased cellular proliferation in the PRP-treated group, compared to the ethanol group. The endometrial areas in the ethanol and PRP-treated groups were 212.83±15.84 μm2 and 262.34±12.33 μm2 (p=0.065). Significantly stronger IHC expression of cytokeratin, homeobox A10 (HOXA10), vascular endothelial growth factor (VEGF), and Ki-67 was found in the PRP-treated group, compared to the ethanol group. In real-time PCR analyses, interleukin-1β mRNA was down-regulated, while c-Kit mRNA was up-regulated, in the PRP-treated group, compared to the ethanolgroup. Conclusion: Intrauterine administration of autologous PRP stimulated and accelerated regeneration of the endometrium and also decreased fibrosis in a murine model of damaged endometrium.

GG-30 : Nontuberculous mycobacteria infection mimicking Fitz-Hugh-Curtis syndrome; case report and literature review

장항용,최양석,김선행,김탁,박현태 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-

Cases of nontuberculous mycobacterial (NTM) infection have been increasing during recent years in immunocompromised and immunocompetent patients. We present the first report of nontuberculous mycobacteria (NTM) infections in the field of gynecology. A 26-year-old nulliparous woman presenting with right upper quadrant and suprapubic pain. She was initially suspected to have Fitz-Hugh-Curtis syndrome (FHCS) but was later diagnosed with extrapulmonary NTM using laparoscopy, which extensive adhesiolysis and ablation of granulomatous lesions were required. Diagnosis of NTM was confirmed by PCR analysis of biopsy specimens showing the presence of acid-fast bacilli. Patients refractory to empirical antibiotic therapy and having negative cervical or vaginal culture results need to be evaluated further to determine the cause of pain. Diagnostic laparoscopy is recommended when other organic disease has been ruled out and medical treatment has failed to relieve symptoms. An awareness of NTM as potential pathogens, even in previously healthy adults, and efforts to exclude other confounding diseases are important to establish the diagnosis of NTM disease. NTM infection can cause a wide range of clinical manifestations, including, as in the present case, severe adhesions and perihepatic inflammation that overlapped the symptoms of FHCS.

클로스트리디움 보툴리눔 독소 A형은 미페프리스톤으로 유발된 조기진통 쥐 모델에서 자궁수축을 현저하게 감소시킨다

장항용 ( Hang Yong Jang ),이경미 ( Kyung Mi Lee ),박영신 ( Young Sin Park ),문명진 ( Myung Jin Moon ),한원보 ( Won Bo Hahn ),안은희 ( Eun Hee Ahn ) 대한산부인과학회 2012 Obstetrics & Gynecology Science Vol.55 No.9

목적: 우리는 클로스트리디움 보툴리눔 독소 A형(BoNT/A)이 미페프리스톤으로 유발된 조기진통 쥐 모델에 대해 자궁수축억제 효과가 있는지 in vivo 연구를 실시하였다. 연구방법: 임신 17일, 개복 후 자궁 양측 뿔에 식염수 또는 20 유니트의 BoNT/A를 투여하였다. 임신 18일, 미페프리스톤을 근주하여 자궁의 조기진통을 유발시켰다. 임신 19일, 근전도검사기를 통하여 자궁수축 활동을 측정하였다. 결과: BoNT/A 20 유니트를 투여한 군은 자궁수축 강도가 BoNT/A를 투여하지 않은 군에 비해 45.2% (P<0.05)로 유의하게 감소하였으며, 자궁수축 기간은 51.6% (P<0.01)로 유의하게 감소하였다. 하지만 자궁수축 빈도에 대한 감소는 5.6% ( P = 0.4)로 유의하게 감소는 없었다. 결론: 미페프리스톤으로 유발된 조기진통 쥐 모델에서 BoNT/A는 자궁의 수축력을 유의하게 감소시켰다. 자궁수축의 감소는 자궁수축 빈도에 대한 억제효과보다는 자궁수축의 기간 및 강도의 억제효과가 유의했다. Objective: We investigated the in vivo tocolytic effect of Clostridium botulinum toxin A (BoNT/A) on mifepristone-induced preterm labor in rats. Methods: On day 17 of gestation, an incision was made to expose the uterus, and BoNT/A (normal saline or 20 units) was injected into the uterine horns, On day 18, mifepristone was used to induce uterine contractions, Electrical activity of uterine contractions was measured via electromyography on day 19, Results: In rats treated with 20 units of BoNT/A, the amplitude of uterine contractions was significantly decreased by 45.2 ± 18.4 (P<0.05) from baseline, respectively. The total duration of uterine contractions was significantly decreased by 51.7 ± 7.9 (P<0.01), respectively. The frequency of contraction bursts after treatment with 20 units of BoNT/A was decreased by 5.6 ± 16.3 from the baseline (P=0.4). Conclusion: In rats undergoing mifepristone-induced preterm labor, BoNT/A significantly inhibited uterine contractility. The decrease in uterine activity was mainly caused by a decline in the duration and intensity rather than frequency of uterine contractions.

RE-05 : The effect of estradiol on adipose tissue inflammation

박현태,장항용,이경욱,김용진,신정호,허준용,김선행,김탁 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-

It is known that premenopausal women have lower risks for cardiovascular disease compared to men, and this sex advantage for women gradually disappear after menopause. However, the mechanisms underlying these sex differeneces are insufficiently understood. Recent studies have demonstrated that inflammation in adipose tissue has strong impacts on cardiovascular diseases. Moreover, macrophage polarization in adipose tissue has grown to become a key player in cardio-metabolic disorder. Our aim was to investigate sex specific response of adipose tissue immune cells to high fat diet and the role of estradiol to adipose tissue inflammation We develpoed C57BL/6J male, non-ovariectomized (SHAM) female, ovariectomized female, and ovariectomized female with estradiol or aromatase inhibitor to receive a normal or high fat diet. High fat diet induced adipose tissue inflammatory markers and macrophage polarization in male mice. Female mice with intact ovary were resistant to HFD induced macrophage polarization. However, T cells (CD4, CD8), dendritic cells and macrophage polarization increased in ovariectomized femlae mice compared to SHAM group. Estradiol supplementation normalized these inflammatory changes wheareas aromatase inhibitor supplementation aggrevated these changes Macrophage polarization and B and T cells, and dendritic cell change by estrogen deficiency may play an important role in developing cardio- metabolic disorder in postmenopausal women.

RE-07 : Estrogen, energy intake, body composition, and glucose metabolism

박현태,류기진,최정민,명수민,장항용,이경욱,김용진,신정호,허준용,김탁 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

목적: As women enter menopause, there is a decline in circulating estrogens. This is accompanied by alterations in energy homeostasis that result in increases in intraabdominal body fat. In animals, ovariectomy (OVX) leads to increased adiposity that is prevented by estrogen replacement. We generated various estrogen sufficient and deficient models to investigate the effects of estrogen on energy intake, body composition, and glucose metabolism. 방법: We randomized C57BL/6 J male, non-ovariectomized female, ovariectomized female, and ovariectomized female mice supplemented with 17β estradiol to receive normal CHOW or a high-fat diet (10-20 mice per group). We generated chemically- induced menopause model by injecting GnRH agonist. We measured weight gained, calories consumed, percent body fat, adipocyte size, nuclear magnetic resonance (NMR), and oral glucose tolerance test (OGTT). 결과: Male mice had a higher susceptibility to obesity than intact female mice. However, removal of the ovaries in female mice eliminated the protection to obesity and estrogen supplementation restored this protection. Estrogen supplementation reduced food intake. So, Pair-feeding strategy was performed to eliminate effect on energy intake of estrogen. NMR showed that estrogen supplementation decreased fat mass and increased lean body mass. There was no difference of OGTT between estrogen group and vehicle group. Chemically-induced menopause model shows that physiological estrogen also affects energy intake and body composition. 결론: Estrogen protects female mice from obesity and preserves lean body mass. However, estrogen supplementation neither improves nor aggravates glucose metabolism in our model.

무월경으로 발현한 라스케열낭 증례

박영신 ( Young Sin Park ),이미화 ( Mee Hwa Lee ),김태헌 ( Tae Heon Kim ),장항용 ( Hang Yong Jang ),이경미 ( Gyong Mi Lee ) 대한산부인과학회 2012 Obstetrics & Gynecology Science Vol.55 No.1

A 28-year-old female presented with Rathke`s cleft cysts (RCC) manifesting as primary amenorrhea and no development of secondary sexual characteristics. She had no symptoms of headache, visual disturbance, anosmia or galactorrhea.. The endocrinologic study demonstrated partial hypopituitarism. Magnetic resonance imaging revealed about 1.5 cm sized sellar and suprasellar cystic tumor, extending into the pituitary stalk. Its tentative diagnosis was benign pituitary cystic tumor, such as RCC. She underwent surgery by a transsphenoidal approach. Histological examination revealed a ciliated columnar epithelium those consistent with RCC. RCC is rarely symptomatic, but mechanical compression by the cyst or infl ammation itself causes headache, visual fi eld defects, or symptoms of pituitary dysfunction. The present case shows that RCC may manifest as hypogonadotropic hypogonadism.

복강경하 자궁각절제술과 자궁경부이형성증 병력있는 환자에서 임신 제3분기에 일어난 연속적인 자궁파열사례

김영란 ( Young Ran Kim ),문명진 ( Myung Jin Moon ),장성운 ( Sung Woon Chang ),안은희 ( Eun Hee Ahn ),장항용 ( Hang Yong Jang ),이경미 ( Gyong Mi Lee ),정상희 ( Sang Hee Jong ) 대한산부인과학회 2012 Obstetrics & Gynecology Science Vol.55 No.12

Uterine rupture during the course of pregnancy is an uncommon obstetric event. Rupture of an unscarred uterus is rare, whereas rupture of a scarred uterus is more common and is usually the result of a Cesarean section. Uterine rupture during pregnancy usually occurs in patient with a scarred uterus, and recurrent uterine rupture usually occurs at a prior ruptured site. However, recurrent uterine rupture that occurs at a different site, regardless of prior rupture site, has yet to be reported. The patient described in this case report had cervical dysplasia with positive for human papilloma virus (HPV) type 16. As the presence of a precancerous lesion in the cervix is reported to increase the risk of preterm birth and spontaneous uterine rupture, a possible correlation between recurrent uterine rupture and cervical dysplasia was considered for this patient.