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Effects of Autologous Platelet-Rich Plasma on Regeneration of Damaged Endometrium in Female Rats
장항용,김용민,명수민,최정민,김탁,천용필,박현태 연세대학교의과대학 2017 Yonsei medical journal Vol.58 No.6
Purpose: To investigate whether autologous platelet-rich plasma (PRP) treatment can improve regeneration of the endometrium in an experimental model of ethanol-induced damage. Materials and Methods: Sixty female Sprague-Dawley rats were randomly assigned into three groups: control group, ethanol group, and PRP-treated group (administration of 0.25 mL of PRP into both uterine cavities 72 hours after ethanol injection). After 15 days of endometrial damage, all the animals were sacrificed during the estrous cycle, and samples were taken from the mid-uterine horn. Functional and structural recovery of the endometrium was analyzed by hematoxylin-eosin (H&E) and Masson trichrome(MT) staining, real-time polymerase chain reaction (PCR) assay, and immuno-histochemical (IHC) analyses. Results: H&E and MT staining confirmed significantly decreased fibrosis and increased cellular proliferation in the PRP-treated group, compared to the ethanol group. The endometrial areas in the ethanol and PRP-treated groups were 212.83±15.84 μm2 and 262.34±12.33 μm2 (p=0.065). Significantly stronger IHC expression of cytokeratin, homeobox A10 (HOXA10), vascular endothelial growth factor (VEGF), and Ki-67 was found in the PRP-treated group, compared to the ethanol group. In real-time PCR analyses, interleukin-1β mRNA was down-regulated, while c-Kit mRNA was up-regulated, in the PRP-treated group, compared to the ethanolgroup. Conclusion: Intrauterine administration of autologous PRP stimulated and accelerated regeneration of the endometrium and also decreased fibrosis in a murine model of damaged endometrium.
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김경대,최정민,명수민,이승현,김민규,최재훈,박현태 한국통합생물학회 2022 Animal cells and systems Vol.26 No.5
Estradiol (E2) treatment has been known to induce changes in food intake, energy expenditure, and weight gain. However, its direct effects on adipose tissue macrophages (ATM) in vivo are not fully understood. Thus, we aimed to explore this aspect at cellular and molecular levels in ovariectomized obese mice. We examined the changes in ATMs after eight weeks of a high-fat diet (HFD) in male, female, and ovariectomized (OVX) mice. After eight weeks, osmotic pumps were inserted into OVX mice to provide two weeks of E2 treatment. We additionally set up a vehicle Pair-Fed (PF) control group that supplied the same amount of HFD consumed by the E2- treated group. We then investigated the in vivo phenotypic changes of visceral adipose tissue (VAT) macrophages. The percentage of M1-like ATMs decreased by the anorectic effect of E2, while M2-like ATMs increased regardless of the anorexia. E2 treatment increased the expression of anti-inflammatory genes but decreased pro-inflammatory genes in VAT. Monocyte recruitment and local proliferation contributed to M2-like ATMs. Furthermore, M2-like phenotypes were induced by E2 treatment in human macrophages. E2 treatment increases M2- like macrophages and improves the tissue milieu of VAT regardless of the anorectic reaction of E2.
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RE-07 : Estrogen, energy intake, body composition, and glucose metabolism
박현태,류기진,최정민,명수민,장항용,이경욱,김용진,신정호,허준용,김탁 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-
목적: As women enter menopause, there is a decline in circulating estrogens. This is accompanied by alterations in energy homeostasis that result in increases in intraabdominal body fat. In animals, ovariectomy (OVX) leads to increased adiposity that is prevented by estrogen replacement. We generated various estrogen sufficient and deficient models to investigate the effects of estrogen on energy intake, body composition, and glucose metabolism. 방법: We randomized C57BL/6 J male, non-ovariectomized female, ovariectomized female, and ovariectomized female mice supplemented with 17β estradiol to receive normal CHOW or a high-fat diet (10-20 mice per group). We generated chemically- induced menopause model by injecting GnRH agonist. We measured weight gained, calories consumed, percent body fat, adipocyte size, nuclear magnetic resonance (NMR), and oral glucose tolerance test (OGTT). 결과: Male mice had a higher susceptibility to obesity than intact female mice. However, removal of the ovaries in female mice eliminated the protection to obesity and estrogen supplementation restored this protection. Estrogen supplementation reduced food intake. So, Pair-feeding strategy was performed to eliminate effect on energy intake of estrogen. NMR showed that estrogen supplementation decreased fat mass and increased lean body mass. There was no difference of OGTT between estrogen group and vehicle group. Chemically-induced menopause model shows that physiological estrogen also affects energy intake and body composition. 결론: Estrogen protects female mice from obesity and preserves lean body mass. However, estrogen supplementation neither improves nor aggravates glucose metabolism in our model.
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