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- 저자 : 임지현(Ji-Hyun Lim) (검색결과 94 건)
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RT-PCR법을 이용한 백합 바이러스 LSV, LMoV, CMV의 검출
임지현,배은혜,이용진,박성한,이규준,김새로미,정용태,Lim, Ji-Hyun,Bae, Eun-Hye,Lee, Yong-Jin,Park, Sung-Han,Lee, Kyu-Jun,Kim, Sae-Ro-Mi,Jung, Yong-Tae 한국미생물학회 2009 미생물학회지 Vol.45 No.3
2008~2009년에 강원, 충남, 제주 지역의 백합 재배 농가에서 바이러스 감염 증상을 보이는 백합의 잎과 구근을 채취하였으며 RT-PCR 방법으로 Lily mottle virus (LMoV), Lily symptomless virus (LSV), Cucumber mosaic virus (CMV) 등 3 종류의 바이러스를 검출 하였다. LSV 12주, LMoV 20주, CMV 1주가 검출 되었으며 LSV에 감염된 12개의 식물체 중 7개에서는 LMoV도 검출되어 복합 감염된 것을 확인하였다. LMoV와 LSV에 의한 복합 감염은 엽맥투명화, 잎말림, 반점, 모자이크, 황화 줄무늬 등 단독 감염보다 심각한 병징을 나타내었으며 좋지 않은 환경에서 저장된 구근에서도 복합 감염이 관찰되었다. 채집된 식물체는 LMoV 감염이 가장 많았으며 Lily virus X(LVX)에 의한 감염은 검출되지 않았다. 7개 분리주의(LMoV 4주, LSV 2주, CMV 1주) 외피 단백질 유전자를 증폭한 후 염기서열을 분석하여 국내에서 발표된 백합 바이러스 염기서열(LSV:AJ516059, CMV: AJ296154)과 비교 하였으며 LMoV는 국내에서 처음으로 염기서열을 결정하여 기존에 보고된 염기서열(AJ564636)과 비교하였다. 분리주는 기존에 보고된 바이러스와 95~99%의 뉴클레오티드 염기서열 유사성을 보였으며, 이들 분리주의 분자 생물학적 특성을 밝히고 신속하고 정확한 바이러스 진단을 위해서는 전체 염기 서열 분석이 필요한 것으로 판단되었다. Leaf samples and bulbs showing characteristic symptoms of virus infection were collected from Gang-won, Chung-nam, and Jeju Province of Korea in 2008-2009. Three viruses, Lily symptomless virus (LSV), Lily mottle virus (LMoV), and Cucumber mosaic virus (CMV) were detected by RT-PCR. Virus-infected plant samples were identified; 12 plants with LSV, 20 plants with LMoV, and 1 plant with CMV. Of the twelve LSV infected samples, seven samples were found to be mix-infected with LMoV and LSV. Symptoms of LMoV and LSV mixed infection were fairly severe, like as vein clearing, leaf curling, leaf mottling, leaf mosaic, and yellow streaking. Mixed infection with LMoV and LSV was also found in lily bulbs which have been stored under unfavorable environmental conditions. LMoV predominated in our tests, whereas spread of Lilyvirus X (LVX) was not found. The nucleotide sequences of coat protein (CP) region of seven isolates (4 LMoV, 2 LSV, and 1 CMV) were compared with the corresponding regions of LMoV (AJ564636), LSV (AJ516059) and CMV(AJ296154). The nucleotide sequence homologies between reference viruses and seven isolates were 95-99%. Complete sequencing of seven isolates is necessary to obtain more information on the molecular characteristics of these viruses as well as to increase sensitivity and rapidity of viral detection.
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임지현 ( Ji Hyun Lim ),오신택 ( Shin Taek Oh ),김형옥 ( Hyung Ok Kim ),김정원 ( Chung Won Kim ),박영민 ( Young Min Park ),송계용 ( Key Young Song ) 대한피부과학회 2003 대한피부과학회지 Vol.41 No.6
Papillary eccrine adenoma is a rare cutaneous tumor which occurs most commonly on the distal portion of extremities as a small solitary nodule. Histopathologically, the tumor consists of multiple dilated or branching tubular structures that are lined by two or more layers of epithelial cells and shows papillary projection into the lumina. Positive staining with S-100 protein, CEA, EMA and keratin in papillary eccrine adenoma is consistent with differentiation toward the secretory epithelium of eccrine sweat gland. Herein we report four cases of papillary eccirine adenoma and review the literature. (Korean J Dermatol 2003;41(6) : 761~765)
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기후변화에 따른 건물의 에너지 소비량 및 최대전력수요 예측
임지현(Ji-Hyun Lim),정호연(Ho-Youn Jung),윤근영(Geun-Young Yun) 대한설비공학회 2013 대한설비공학회 학술발표대회논문집 Vol.2013 No.11
The purpose of this paper is to predict the energy consumption and peak demand for electricity in a building under climate change using building energy simulation software OpenStudio based on EnergyPlus for a typical open plan office in Seoul, Korea. After the building energy simulation, annual cooling and heating energy consumption and peak demand for electricity were calculated. The result shows that building energy consumption is related to the climate change. Also, summer peak demand for electricity was the highest ever simulated with Seoul weather data in 2080. Heating energy consumption shows a tendency to decrease and cooling energy consumption is increasing every 30 years. These results vary from depending on scenarios of GHG emissions.
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공감하고 실천하는 민주시민을 기르는 역사수업 - ‘정서적 감정이입(affective empathy)’을 적용한 수업 방안 모색 -
임지현 ( Lim Ji-hyun ) 역사교육학회 2021 역사교육논집 Vol.76 No.-
Recently, the newly perceived historical consciousness is related to the students' proper awareness of the historical facts of the past and their interest in the historical issues that have led to the present, and to foster the power to make the better future history on their own. Also, it is to have a citizen's consciousness to make a right decision on a given problem and to practice it at the same time, and to cultivate such a democratic citizen's historical consciousness may be a purpose of history education. involves students' interest in the historical issues that have led to the present, while fully aware of the historical facts of the past, and furthermore, I will develop the power to make the better future history on their own. Meanwhile, Barton and Levstik, who emphasized democratic citizenship education in that history education should contribute to promoting common good and participatory democracy, insisted that historical empathy is a useful principle of instruction for cultivating citizenship. In particular, criticizing the emphasis on only cognitive inquiry, they suggested that affective factors such as empathy and identification should be actively combined into historical empathy. Barton and Levstik's views have been developed by researchers at local and abroad into the discussion of the re-conceptualization to properly combine historical empathy with the cognitive inquiry processes and affective elements. It is clear that these discussions are possible logic when integrating concepts based on the studies of scholars and teaching strategies of teachers. In addition to the discussion of re-conceptualization of historical empathy and analysis of class cases using sympathy and emotion, this study specifically designed a teaching plan based on the hypothesis that historical empathy applying affective dimension can greatly contribute to the growth of students as democratic citizens, and tried to find the possibility. At the same time, through the class, this study tried finding that Barton and Levstik's ‘affective empathy’ called ‘caring’ embodies the driving force that students lead the knowledge and consciousness of history learned in classes to the practice of action as the core of the civic competency. In conclusion, historical empathy should be conceptualized as the principle of instruction that combines both tools of cognitive inquiry and affective elements. Especially, the application of ‘affective empathy’ called ‘caring’ is a factor that exerts more aggressive sympathy among students, and it could be used as a key teaching principle of democratic civic education in that it plays a role in promoting interest in the ‘common good’ such as human rights, peace, and coexistence.
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미용 종사자 교육 정도의 소비자 인식 여부에 따른 헤어스타일별 적정 시술 시간 만족에 관한 연구
임지현 ( Ji-hyun Lim ),김동표 ( Kim Dong Pyo ),박은준 ( Eun-jun Park ) 한국미용예술경영학회 2011 미용예술경영연구 Vol.5 No.1
The structure of industry in Korea has been changing to that of service industry. The quantitative growth of service industry raises competition among service enterprises but does not yet reach qualitative improvement. In this environment in which service industry undergoes quantitative growth, qualitative enhancement of beauty industry employees who can provide customer satisfaction and customer impression services is recognized as the crucial core factor in the development of beauty care companies, and in that beauty industry, too. Thereupon, in order for the beauty care companies to provide information and academic data that can satisfy customer' s eye level by establishing service strategies, this study divides consumers into those who recognize the educational degree of beauty industry employees and those who do not with the questionnaire method and examines the satisfaction with the operation time by hairstyle according to the recognition on the educational degree of the employees. As a result, about the sex ratio of the study subjects, females were more than males. And the jobs included specialized jobs, office jobs, students, independent businessmen, and salespersons in order. Their monthly income was mostly from 2 million to 3 million won, and those with high income over 4 million won were quite rare. Among the subjects, the married were more than the unmarried. Regarding the level of education, most of them were attending or graduating from college or attending graduate school or higher, and those graduating from high school or lower were quite rare. It is considered that this is correlated with the social trend of high-level education. Regarding the consumer recognition on the educational degree of beauty industry employees, the recognition group (that recognizes the educational degree of beauty industry employees) was more than the non-recognition group(that does not recognize the educational degree of beauty industry employees). About the satisfaction with the operational time by hairstyle according to the recognition group and non -recognition group, the recognition group was more than the non -recognition group. With regard to the satisfaction with the optimal operation time by hairstyle between the recognition group and non-recognition group, the male permanent style showed statistica11y significant difference in shadow perm and the non-recognition group did so separately in shadow perm and shaggy perm. And the female permanent style indicated significant difference in the hot perm style’ s magic, setting, and digital, and the general perm style’ s texture, baby, and roll straight. Socially, they think much theoretical education is needed for permanent styles. Male' s cut-style, up-style, dry-style, and dye-style did not indicate significant difference. The result is inferred to be drawn from misinterpretation that both the recognition group and the non-recognition group related it with sensual expression rather than theoretical education, so it is thought that this does not affect the operational time. In conc1usion, consumers do not spare their investment to manage their personal value and image once they have security and influence socia11y. Thus, it is deemed that if beauty industry employees who have completed proper education right for their consumer' s value and ability enhance their value by delivering their value and capacity to their consumers properly, this wi11 lead to the increment of sales and the increase of faithful costumers as we11.
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