유전체장벽 방전구조의 비접촉식 저온 대기압 면방전 플라즈마를 이용한 빵곰팡이의 살균효과

유영효,엄환섭,박경순,최은하,Ryu, Young Hyo,Uhm, Han Sup,Park, Gyung Soon,Choi, Eun Ha 한국진공학회 2013 Applied Science and Convergence Technology Vol.22 No.2

본 연구에서는, 동시에 넓은 면적을 조사할 수 있는 형태의 유전체 장벽 방전을 이용한 대기압 면방전 플라즈마 발생장치를 이용하여 곰팡이의 살균 실험을 수행하였다. 실험에 사용한 면방전 플라즈마 발생장치의 파워는 사인파 교류전원을 이용하였으며, 1.4~2.3 kV의 방전전압을 가진다. 또한, 유전체 전기용량의 특성으로 인하여 전압과 전류의 위상차는 약 80도를 갖는다. 생물시료에 미치는 온도의 영향은 공랭식 쿨러를 사용하여 유전체의 열을 배출함으로써 최소화 하였으며, 시료의 온도는 온도측정장치를 사용하여 쿨러(Cooler) 작동 시 최대 10분간 플라즈마를 발생시켜도 37도가 넘지 않음을 확인하였다. 플라즈마에서 생성되는 활성종중 오존($O_3$)의 양은 플라즈마 발생부로부터 1 cm 이내에서 약 25~30 ppm이 측정되었으며, 150 cm 떨어진 지점에서도 5 ppm 정도 측정되었다. 그에 비해 일산화질소(NO)나 이산화질소($NO_2$)는 거의 검지되지 않음을 확인하였다. 증류수 속에 담긴 빵 곰팡이 포자를 면방전 플라즈마 발생장치로 처리하였을 때, 포자의 발아율은 처리시간 및 출력파워가 증가함에 따라 급격히 감소하였으며, MTT (3-(4,5-dimethy lthiazol-2yl)-2,5-diphenyl-2H-tetrazolium bromide) 측정법을 통해 분석한 미토콘드리아 활성도도 처리시간과 출력파워에 비례하여 감소함을 보았다. 반면 포자를 Vogel's minimal 배양액에 넣고 플라즈마 처리를 하면, 앞의 실험과 달리 살균효과가 미비함을 보였는데, 이를 통해 포자를 둘러싸고 있는 환경이 플라즈마의 살균효과에 영향을 미치는 것으로 보여진다. 본 연구를 통하여 유전체 장벽을 이용한 면방전 플라즈마 발생장치는 플라즈마 제트(jet)와는 달리 직접적인 접촉 없이도 미생물 살균이 가능하다는 것을 보여주었으며, 이는 면방전 플라즈마 발생장치로부터 발생하는 활성종들이 곰팡이와 같은 미생물의 비활성화에 주요역할을 하기 때문이라고 본다. Sterilization of Neurospora crassa has been investigated in this research by using a surface air plasma with dielectric barrier discharged (DBD) structure under atmospheric pressure. The sinusoidal alternating current has been used in this experiment with discharge voltage of 1.4~2.3 kV. The phase difference between the voltage and current signals are found to be almost 80 degree due to the capacitive property of dielectric barrier. Temperature on the biomaterials has been minimized by radiating the heat with the air cooling system. It is noted that the substrate temperature remains under 37 degree for plasma exposure time of 10 minutes with operation of cooler system. It is found that the ozone, $O_3$, has been measured to be about 25~30 ppm within 1 cm region and to be about 5 ppm at the 150 cm downstream region away from the suface plasma. It is also noted that the nitric oxide, NO, and nitric dioxide, $NO_2$, are not nearly detected. Germination rate and mitochodrial activity of Neurospora crassa immersed in the deionized water have been found to be drastically decreased as the plasma treatment time and its electrical power are increased in this experiment. Here, the mitochondrial activity has been analyzed by MTT (3-(4,5-dimethy lthiazol-2yl)-2,5-diphenyl-2H-tetrazolium bromide) assay. However, sterilization of Neurospora crassa immersed in the Vogel's minimal media has been found to be low by plasma treatment, which is caused by surrounding background solution. This research shows the sterilization possibility of Neurospora crassa by using the noncontated surface DBD plasma, which is different from the plasma jet. This is mainly attibuted to the reactive species generated by the surface plasma, since they play a major role for inhibition of micobes such as Neurospora crassa.

임신능이 확인된 가임 여성에서의 Deciduosis의 유병율

김미란,유영옥,노덕영,류순원,권동진,김장흡,김진홍,임용택,김은중,정재근,이진우,Kim, Mee-Ran,Lew, Young-Oak,Ro, Duck-Yeong,Ryu, Sun-Won,Kwan, Dong-Jin,Kim, Jang-Heub,Kim, Jin-Hong,Lim, Yong-Taik,Kim, Eun-Jung,Jung, Jae-Keun,Lee, Jin-Woo 대한생식의학회 2000 Clinical and Experimental Reproductive Medicine Vol.27 No.4

Objective: Extrauterine formation of decidua of stromal cells has been well described, particularly in the cervix and ovary. The apparent hormonal mechanisn of this phenomenon suggestes a relationship to endometriosis. Whether formation of ectopic decidua represents a marked progestational response of endometriosis or an independent peritoneal-stromal reaction to pregnancy is unclear. This study was designed to determine the prevalence of deciduosis in the patients whose fertility were proven. Design: Prospective study of patients who had undergone cesarean delivery without history of endometriosis. Materials and Methods: The study was performed in 179 full tenn pregnant women. During the cesarean section, the pelvic organs were thoroughly investigated and the biopsies were collected at the lesions suspicious endometriosis. And then microscopic examination of removed tissues were done. Results: Of the 179 patients who underwent cesarean delivery, 48 women (26.8%) had the lesions suspicious endometriosis such as adhesion, pigmented spots. The ovary was the most frequently ocurred site (79.2%). Microscopically, decidual cells were observed in 34 cases (70.8%) of 48 biopsed patients. Conclusion: Endometriosis has been known to be associated with subfertility. Our observations found the prevalence of deciduosis was 19.0% (34/179) in tenn pregnant women whose fertilites were proven. We suggests that the deciduosis maya manifestation of endometriosis during pregnancy. However, further follow up study should be done to confirm this clinicopathologic process.

요인 및 군집분석을 이용한 유해화학물질 사고 잠재적 피해에 대한 도시 유형 분류 및 특성 분석

이승훈(Seung Hoon Lee),유영은(Young Eun Ryu),김규리(Kyu Ri Kim),백종인(Jong In Back),김호현(Ho-Hyun Kim),반영운(Yong Un Ban) 한국환경보건학회 2020 한국환경보건학회지 Vol.46 No.6

Objectives: The aim of this study was to analyze and classify the characteristics of potential damage from hazardous chemical accidents in 229 administrative units in South Korea by reflecting the social and environmental characteristics of areas where chemical accidents can occur. Methods: A number of indicators were selected through preceding studies. Factor analysis was performed on selected indicators to derive factors, and cluster analysis was performed based on the factor scores. Results: As a result of the cluster analysis, 229 administrative units were divided into three clusters, and it was confirmed that each cluster had its own characteristics. Conclusions: The first cluster, “areas at risk of accident occurrence and spread of damage” was a type with a high potential for accident damage and a high density of hazardous facilities. The second cluster, “Urban infrastructure damage hazard areas” appeared to be a cluster with high urban development characteristics. Finally, the third cluster ‘Urban and environmental damage hazard areas’ appeared to be a cluster with an excellent natural environment. This study went further from the qualitative discussion related to existing chemical accidents to identify and respond to accident damage by reflecting the social and environmental characteristics of the region. Distinct from the previous studies related to the causes of accidents and the response system, it is meaningful to conduct empirical research focusing on the affected areas by analyzing the possibility of accident damage in reflection of the social and environmental characteristics of the community.

석탄을 이용한 합성천연가스(SNG)의 최근 생산기술 현황

강석환(Suk-Hwan Kang),류재홍(Jae-Hong Ryu),김수현(Su-Hyun Kim),김진호(Jin-Ho Kim),김효식(Hyo Sik Kim),정기진(Ki-Jin Jeong),이지은(Ji-Eun Lee),유영돈(Young-Don Yoo),고동준(Dong-Jun Koh) 한국에너지기후변화학회 2014 에너지기후변화학회지 Vol.9 No.1

As the world-wide natural gas demand increases gradually, the projects for producing the synthetic natural gas (SNG) from coal are attracting much attention because they are economical and reliable. However, there are still a lot of problems to overcome, such as the emergence of shale gas, coal price increase and the greenhouse gas emission during this process. In this work, the methods for producing SNG from coal and related catalytic reaction processes are introduced, and the recent progress in commercial SNG projects is described. In addition, the research trend ion the Bio-SNG project drawing attention in Europe is also discussed.

남한의 생물기후권역 구분과 특성 규명

최유영 ( Choi Yu-young ),임철희 ( Lim Chul-hee ),류지은 ( Ryu J I-eun ),( Dongfan Piao ),강진영 ( Kang J In-young ),( Weihong Zhu ),( Guishan Cui ),이우균 ( Lee Woo-kyun ),전성우 ( Jeon Seong-woo ) 한국환경복원기술학회(구 한국환경복원녹화기술학회) 2017 한국환경복원기술학회지 Vol.20 No.3

This study constructed a high-resolution bioclimatic classification map of South Korea which classifies land into homogeneous zones by similar environment properties using advanced statistical techniques compared to existing ecological area classification studies. The climate data provided by WorldClim(1960-1990) were used to generate 27 bioclimatic variables affecting biological habitats, and key environmental variables were derived from Correlation Analysis and Principal Component Analysis. Clustering Analysis was performed using the ISODATA method to construct a 30`(~1km) resolution bioclimatic classification map. South Korea was divided into 21 regions and the results of classification were verified by correlation analysis with the Gross Primary Production(GPP), Actual Vegetation map made by the Ministry of Environment. Each zones` were described and named by its environmental characteristics and major vegetation distribution. This study could provide useful spatial frameworks to support ecosystem research, monitoring and policy decisions.

보호지역의 지정 및 관리를 위한 국립공원의 서식처 기능 평가 一생물종다양성과 서식처 가치에 기반하여一

류지은 ( Ryu Ji-eun ),최유영 ( Choi Yu-young ),전성우 ( Jeon Seong-woo ),성현찬 ( Sung Hyun-chan ) 한국환경복원기술학회(구 한국환경복원녹화기술학회) 2018 한국환경복원기술학회지 Vol.21 No.5

National parks are designated for the purpose of maintenance, conservation and utilization of different habitats. Therefore, it is necessary to select habitats of a high value as a protected area in order to balance conservation and development. However, the existing national park boundary adjustment and new designation criteria only focused on the endangered species and protected area, without proper evaluation of the habitat value of actual species. Therefore, this study aims to quantitatively evaluate habitat function in terms of biodiversity and habitat value, so that it can be referred to for the designation and boundary adjustment of national parks. We assessed species diversity and habitat values for each of the habitat types, for mammals only, as they are able to choose preferred habitats. In order to evaluate biodiversity, we used Maxent to derive species richness map and used InVEST's Habitat quality model to evaluate habitat value. As a result of evaluation, species richness was high in the national park boundary area. Also, even if the same edge is adjacent to the development area depending on the land cover, the species richness is low. Compared with Wolaksan and Sobaeksan National Park, the species richness and habitat value of the northern area, which is connected with other forests, were higher than those of the southern area where roads were developed. Therefore, it is expected that the use of the result of this study for the national park boundary adjustment and management will enhance the function of the national park as a habitat.