시판 정유의 성분 분석과 다약제 내성균에 대한 항균활성 및 항산화 효능 평가

유영월(Yeong wol Yoo),이효정(Hyo Jeong Lee),김승(Seung Kim),배민석(Min Suk Bae),이미자(Mi Ja Lee),심정현(Jung-Hyun Shim),조승식(Seung Sik Cho) 한국생명과학회 2014 생명과학회지 Vol.24 No.3

본 연구에서는 국내 시판 중인 정유의 화학적 구성, 항균 및 항산화 효과를 조사하였다. 정유의 성분 분석은 GC-MS법을 이용하였다. 정유는 그람음성, 양성 및 다약제 내성균에 대하여 광범위한 항균 범위를 보여주었다. 정유 5종의 항균활성은 40개 병원성 세균에 대한 MIC로 측정하였다. Lemongrass와 manuka는 0.0625-0.5% (v/w), tea tree는 0.03125-1.0% (v/w), thyme 0.0625-1.0% (v/w) 및 ravensara는 1-4% (v/w)의 MIC 수치를 나타내었다. 정유의 항산화 활성은 환원력 및 DPPH법으로 평가하였다. 5종의 정유 모두 농도 의존적으로 DPPH free radical 저해 및 환원력을 가지고 있었으며, thyme이 대조군인 ascorbic acid와 비교 시 가장 높은 항 산화능을 보였다. 5종의 정유 모두 고른 항균 및 항산화능을 가지고 있으며, 이중 thyme이 가장 높은 항균활성 및 항산화능을 가지고 있어 천연 항균 항산화제로써의 가치가 있다고 생각되었다. 본 연구진은 국내 시판중인 정유 5종의 성분분석, 항균 및 항산화 활성을 처음으로 비교 분석하여 보고하였다. In the present study, the chemical compositions and antimicrobial and antioxidant activities of commercially available essential oils in Korea were investigated. The essential oils were analyzed by gas chromatography-mass spectrometry (GC-MS). The results demonstrated that they exhibit a broad spectrum of antimicrobial activities against gram positive, gram negative, and multidrug resistant (MDR) strains. The antimicrobial activity of five of the essential oils against 40 organisms was assessed using the minimum inhibitory concentration (MIC). The MIC values were in the ranges of 0.0625-0.5% (v/w) for lemongrass and manuka, 0.03125-1.0% (v/w) for tea tree, 0.0625-1.0% (v/w) for thyme, and 1-4% (v/w) for ravensara, depending on the pathogens studied. This study revealed that, among the essential oils tested, lemongrass and thyme oil showed broad antimicrobial activity against infectious bacteria. The antioxidant activities and the reducing power of the essential oils were determined with a 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay. Thyme oil exhibited the strongest antioxidant activity comparing with ascorbic acid. This is the first report on the chemical compositions and antimicrobial activities of commercially available essential oils against infectious bacteria and MDR strains acquired from Korean hospitals.

배양 인체피부섬유모세포에서 접촉 피부염 알러젠인 니켈에 대한 차즈기 추출물의 보호 효과

이재규,유영월,양현옥 한국피부과학연구원 2012 대한피부미용학회지 Vol.10 No.4

To clarify the cytotoxicity and protective effect of Perilla silkokiana Nakai (PSN) extract on nickel, an allergen of contact dermatitis (CD), cell viability was analysed by XTT assay after human skin fibroblasts (Detroit 551) were cultured in media containing various concentrations of nickel sulfate (NiSO4). And also, the effect of antioxidant enzyme, catalase (CAT) on NiSO4-induced cytotoxicity was examined. For the protective effect of PSN extract on NiSO4-induced cytotoxicity, Detroit 551 cells were pretreated with 50 or 70 μg/㎖ of PSN extract for 2 h before the treatment of NiSO4. And also, the antioxidative effects of PSN extract were assessed by DPPHradical scavenging activity and the inhibitory activity of lipid peroxidation. In this study, NiSO4 significantly decreased cell viability dose-dependently compared with control. And the XTT50 value was determined at 110μM of NiSO4. In the effect of antioxidant enzyme, catalase effectively prevented NiSO4-induced cytotoxicity by the significant increase of cell viability. In the protective effect of PSN extract on NiSO4-induced cytotoxicity,PSN extract significantly increased cell viability which was decreased by NiSO4-induced cytotoxicity, and also it showed the antioxidative effects such as DPPH-radical scavenging activity and the inhibitory activity of lipid peroxidation. From these results, it is suggested that the cytotoxicity of NiSO4 was involved in oxidative stress,and also, PSN extract effectively prevented the cytotoxicity induced by allergen of CD, NiSO4 by antioxidative effect. Conclusively, PSN extract may be a putative resources as an antioxidant for oxidative stress-mediated skin disease via allergen of CD.

배양 신경교종세포에서 치매유발물질인 알루미늄의 세포독성 및 쇠비름 추출물의 보호효과

김선주,유영월,이재규 한국인간·식물·환경학회 2013 인간식물환경학회지 Vol.16 No.5

치매유발제인 알루미늄(aluminum, Al)에 대한 세포독성과 이에 대한 쇠비름(Portulaca oleracea L., PO)추출물의 영향을 배양 신경교종세포(C6 glioma)를 재료로 항산화 측면에서 조사하여 다음과 같은 결과를 얻었다. 본 연구에서 염화알루미늄(AlCl3)을 배양 C6 glioma세포에 처리한 결과 처리 농도에 비례하여 세포생존율을 유의하게 감소시킴으로서 세포독성을 나타냈으며 XTT50값이 100uM 이상으로 나타남으로서 중간독성(mid-toxic)인 것으로 나타났다. 한편, 항산화제인 vitamin E는 AlCl3에 의하여 감소된 세포생존율을 유의하게 증가시킴으로서 AlCl3에 의한 세포독성을 방어하였다. 한편, AlCl3의 세포독성에 대한 PO추출물의 영향에 있어서, PO추출물은 AlCl3에 의하여 감소된 세포생존율을 유의하게 증가시킴으로서 AlCl3에 의한 세포독성을 방어하였으며, 또한 superoxide dismutase (SOD)-유사활성(SOD-like activity)과 지질과산화(lipid peroxidation, LP)억제능 및 lactate dehydrogenase (LDH)활성 감소를 나타냈다. 이상의 결과에서 AlCl3의 세포독성에 산화적 손상이 관여하고 있으며 PO추출물은 AlCl3에 의한 세포독성을 항산화 측면에서 유효하게 방어하였다. 따라서 PO와 같은 천연추출물은 산화적 손상에 의하여 매개되는 치매와 같은 병변의 예방이나 치유를 위한 소재로서의 활용성이 클 것으로 생각된다. To evaluate the cytotoxicity of dementia-induced agent, aluminum(Al) and the protective effect of Portulaca oleracea L.(PO) extract on the Al-induced cytotoxicity in cultured neuroglioma cells(C6 glioma). Cultured C6 glioma cells were treated with aluminum chloride(AlCl3) or PO extract. In this study, AlCl3 significantly decreased cell viability compared with control, and XTT50 was determined at a concentration of 121.5uM. Therefore, AlCl3 was midcytotoxic by the criteria of Borenfreund and Puerner. While, AlCl3-induced cytotoxicity was prevented by vitamin E, an antioxidative enzyme. In the protective effect of PO extract on AlCl3-induced cytotoxicity, PO extract prevented AlCl3-induced cytotoxicity by the significant increase of cell viability. And also, PO extract showed antioxidative effects such as superoxide dismutase(SOD)-like activity, the inhibitory activity of lipid peroxidation(LP) and the decrease of lactate dehydrogenase(LDH) activity. it is suggested that AlCl3 was midcytotoxic to cultured C6 glioma cells and the cytotoxicity was involved in oxidative stress. PO extract was effective in the prevention of AlCl3-induced cytotoxicity by the antioxidative effect. Therefore, natural component such as PO extract may be a putative resources for preventing reactive oxygen species(ROS)-related diseases such as dementia or stroke.

보문: Chromium Trioxide로 유발된 세포손상에 대한 Lavender 오일의 항산화 효과

박승택 ( Seung Taeck Park ),유영월 ( Young Wall Ryu ),김선주 ( Sun Ju Kim ),김지원 ( Ji Won Kim ),서영미 ( Young Mi Seo ),박미순 ( Mi Soon Park ),김은주 ( Eun Ju Kim ),이화정 ( Hwa Jeong Lee ),정인주 ( In Ju Jung ) 대한미용학회(구 대한미용과학회) 2011 대한미용학회지 Vol.7 No.2

The cell viability by XTT assay, DPPH-radical scavenging activity, superoxide dismutase (SOD)-like activity and lipid peroxidation were measured to evaluate the cell injury of chromium trioxide (CrO3) and the antioxidative effect of lavender (Lavendula angustifolia L.) oil on the cell injury induced by CrO3 in cultured C6 glioma cells. C6 glioma cells were then treated with the media containing 30~50 μM of CrO3 for 48 hours. The results of this study showed that CrO3 remarkably decreased cell viability in dose-dependent manner and the XTT50 value was appeared at 40.5 μM of CrO3. In the effect of antioxidant, SOD on CrO3-mediated cytotoxicity significantly increased the cell viability which was decreased by CrO3. Meanwhile, in the protective effect of lavender oil on the cytotoxicity induced by CrO3, it remarkably increased the cell viability damaged by CrO3-mediated cell injury and also showed the DPPH-radical scavenging activity, SOD-like activity and the inhibitory activity of lipid peroxidation. From these results, it is revealed that the cell injury by CrO3 was involved in oxidative stress and lavender oil was effective in the protection of CrO3-mediated cell injury by antioxidative effect.

배양 인체 피부 흑색종세포에 대한 제라늄오일의 항산화 활성 및 멜라닌 합성에 미치는 영향

박승택 ( Seung Taeck Park ),유영월 ( Young Wall Ryu ),김선주 ( Sun Ju Kim ),이귀영 ( Gui Yeong Lee ),정다정 ( Da Jung Jung ),장병수 ( Byung Soo Chang ),범희주 ( Hee Ju Beom ),이화정 ( Hwa Jeong Lee ),정인주 ( In Ju Jung ) 대한미용학회(구 대한미용과학회) 2011 대한미용학회지 Vol.7 No.3

For the purpose of searching for whitening resources using essential oil of herb, the antioxidative activity and melanogenesis of Geranium (Palargonium graveolens) oil were examined in cultured human skin melanoma cells (SK-MEL-3). the cell viability, superoxide dismutase (SOD)-like activity, inhibitory activity of lipid peroxidation, tyrosinase activity and total amount of melanin were measured by colorimetric assay. For this experiment, SK-MEL-3 cells were treated with the media containing 25~55 μU/mL, glucose oxidase (GO) for 4 hours, and assessed for the cytotoxicity. GO significantly decreased cell viability compared with control and XTT50 value was determined at 45 μU/mL of GO. In the antioxidative activity of Geranium oil, it showed SOD-like activity and the inhibitory activity of lipid peroxidation. While, in the melanogenesis of Geranium oil, Geranium oil significantly decreased the tyrosinase activity and total amount of melanin which were increased by GO-induced cytotoxicity. From these results, it is suggested that Geranium oil was effective in the protection of GO-induced cytotoxicity by antioxidative activity and inhibitory effect of melanogenesis. Finally, an essential oil such as Geranium oil may be a putative agent for the improvement of whitening effect.

모발염색제 성분인 초산납에 대한 어성초 추출물의 항산화 및 미백효과

정재윤,오영희,박신희,윤미영,표애자,김선주,유영월,정인주 대한미용학회 2014 대한미용학회지 Vol.10 No.2

To evaluate the antioxidative and whitening effects of Houttuynia cordata Thunb (HCT) extract on lead acetate (LA),hair dye component, cell viability was measured by colorimetric assay after NIH3T3 fibroblasts were cultured in mediacontaining various concentrations of LA. And also, the effect of antioxidant, vitamin E on LA-induced cytotoxicity wasanalysed. For the protective effect of HCT extract on LA-induced cytotoxicity, NIH3T3 fibroblasts were pretreated with110 or 130 μg/mL of HCT extract for 2 h before the treatment of LA. And also, the antioxidative and melanogenic effectsof HCT extract were assessed by superoxide dismutase (SOD)-like activity, total amount of melanin and tyrosinase activity. In this study, LA significantly decreased cell viability in dose-dependent manner. And the XTT50 value was calculated at48.3 μM of LA. In the effect of antioxidant, vitamin E, it effectively prevented LA-induced cytotoxicity by the significantincrease of cell viability. In the protective effect of HCT extract on LA-induced cytotoxicity, HCT extract significantlyincreased cell viability which was decreased by the cytotoxicity induced by LA, and also it showed the antioxidative effectby SOD-like activity. In the melanogenesis, HCT extract showed the decreased of amount of melanin and tyrosinase activity. From these results, it is suggested that the cytotoxicity of LA is involved in oxidative stress, and HCT extract effectivelyprevented the cytotoxicity and the melanogenesis induced by LA. 삼백초과(Saururaceae)에 속하는 어성초(Houttuynia cordataThunb, HCT) 추출물이 모발염색제 성분인 초산납(LA)에 미치는 영향을 배양 NIH3T3 섬유모세포를 재료로, 항산화와 미백효과를 조사하였다염모제 성분인 LA는 배양 NIH3T3 섬유모세포에 처리한농도에 의존적으로 세포생존율을 유의하게 감소시켰고(p⁄0.001) 이 과정에서 LA의 XTT50값은 48.3 μM에서 나타났다. 또한 항산화제인 vitamin E는 LA에 의하여 감소된 세포생존율을 유의하게 증가시킴으로서 LA의 세포독성을 방어하였다. LA의 세포독성에 대한 HCT 추출물의 영향에 있어서, HCT 추출물은 LA만의 처리에 비하여 세포생존율을 유의하게 증가시켰으며(p⁄0.001), 이와 동시에 SOD-유사 활성을 보임으로서 항산화능을 나타냈다. 멜라닌화에 있어서,HCT 추출물은 LA에 의하여 증가된 티로시나제(tyrosinase)활성과 총멜라닌양을 모두 유의하게 감소시켰다(p⁄0.001). 이상과 같이 염모제 성분인 LA의 세포독성에 산화적 손상이 관여하고 있으며, HCT 추출물은 항산화능에 의하여LA의 세포독성을 방어하였다. 또한 HCT 추출물은 티로시나제 활성과 총멜라닌양을 유의하게 감소시킴으로서 멜라닌화를 억제하는 데 효과적인 것으로 나타났다. 따라서 HCT추출물과 같은 천연성분은 LA와 같이 산화적 손상이나 멜라닌화와 관련이 있는 모발염색제에 대한 독성경감이나 미백을 위한 미용소재로서의 개발적 가치가 크다고 생각된다.

보문 : 염모제 성분인 카드뮴의 세포독성에 대한 시엽추출물의 영향

손영우 ( Young Woo Son ),임요섭 ( Yo Sup Rim ),유영월 ( Yeong Wol Yu ),정인주 ( In Ju Jung ) 대한미용학회(구 대한미용과학회) 2012 대한미용학회지 Vol.8 No.1

This study was carried out to investigate the cadmium (Cd)-induced cytotoxicity and the protective effect of persimmon leaves (PL) extract on the toxic effect induced by cadmium chloride (CdCl2) in cultured human skin fibroblasts (Detroit 551). In this study, CdCl2 showed the cytotoxicity by a significant decrease of the cell viability and cell adhesion activity (CAA). While, vitamin E as antioxidant protected CdCl2-induced cytotoxicity by the increase of CAA which was decreased by CdCl2. In the protective effect of PL extract against CdCl2-induced cytotoxicity, PL extract blocked CdCl2-induced cytotoxicity by the a significant increase of CAA as well as superoxide dismutase (SOD)-like activity and inhibitory activity of lipid peroxidation (LP). From these results, it is suggested that the cytotoxicity of CdCl2 is involved in oxidative stress, and PL extract showed the antioxidative effect by showing not only a significant increase of CAA but also SOD-like activity and inhibitory activity of LP. Conclusively, the physioactive components such as PL extract may be a putative beauty resources for the diminution or blocking the cytotoxicity or oxidative stress mediated by heavy metals or chemicals which are the materials of hair dyes or cosmetics.

보문 : Hydrogen Peroxide로 손상된 배양 인체피부섬유모세포에 대한 함초추출물의 항산화 및 항독효과에 관한 연구

최유선 ( Yu Sun Choi ),김선주 ( Sun Ju Kim ),전명옥 ( Myung Ok Jeon ),유영월 ( Young Wall Ryu ),임요섭 ( Yo Sup Rim ),정인주 ( In Ju Jung ) 대한미용학회(구 대한미용과학회) 2011 대한미용학회지 Vol.7 No.4

This study aims to evaluate the antioxidative effect and the detoxic effect of Salicornia herbacea L. (SH) extract. For the examining of antioxidative effect on SH extract, the cell viability of freeze-dried SH (FSH) extract or cold-dried SH (CSH) extract was measured after the cultured human skin fibroblasts (Detroit 551) were pretreated with SH extract for 2 hours before the treatment of hydrogen peroxide (H2O2) and also the inhibitory activity of lipid peroxidation was assessed. In the detoxic effect of SH extract, the cell viability was measured by colorimetric assay after Detroit 551 cells were pretreated with FSH extract or CSH extract before the treatment of H2O2. In this study, H2O2 significantly decreased cell viability in dose-dependently and the XTT90 and XTT50 values were determined at 15 μM and 40 μM of H2O2, respectively. In the protective effect of FSH extract against H2O2-treated group, FSH extract significantly increased cell viability, compared with H2O2-treated group, and also FSH extract showed the inhibitory activity of lipid peroxidation. While, in the detoxic effect of SH extract, FSH extract significantly increase cell viability which were decreased by methylmercuric chloride (MMC)-induced cytotoxicity, but CSH extract did not show any protective effect. From these results, it is suggested that H2O2 was highly toxic and SH extract such as FSH extract was effective in the prevention of H2O2- or MMCinduced cytotoxicity by antioxidative effect or detoxic effect. Conclusively, FS extract may be a useful agent for the beauty materials by the protective effect on a bleaching agent, H2O2 or the heavy metal as the pigment of color make-up product

Cisplatin의 세포독성에 대한 레몬밤 추출물의 억제 효과

최유선 ( Yu Sun Choi ),김선주 ( Sun Ju Kim ),유영월 ( Yeong Wol Yu ),임요섭 ( Yo Sup Rim ),황은희 ( Eun Hee Hwang ),이화정 ( Hwa Jeong Lee ),최은영 ( Eun Young Choi ),장병수 ( Byung Soo Chang ),정인주 ( In Ju Jung ) 대한미용학회(구 대한미용과학회) 2013 대한미용학회지 Vol.9 No.1

The purpose of this study is to evaluate the protective effect of Lemon balm (Mellisa officinalis) extract on the cytotoxicity induced by cisplatin, pharmaceutic alopecia inducer. To achieve this, cisplatin-induced cytotoxicity was assessed by XTT assay after human skin fibroblasts (Detroit 551) were cultured in media containing various concentrations of cisplatin. And also, the effect of vitamin E was examined on the cisplatin-induced cytotoxicity. For the protective effect of Lemon balm extract on cisplatin-induced cytotoxicity, Detroit 551 cells were pretreated with 80 or 100 μg/mL of Lemon balm extract for 2 hours, and also, antioxidative effects of Lemon balm extract such as 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical scavenging activity and lactate dehydrogenase (LDH) activity were analysed. The results were as follows. Cisplatin showed a significant decrease of cell viability in dose dependently, and the XTT50 value was calculated at 25.7 μM. In the effect of vitamin E, it significantly increased cell viability which were decreased by the cisplatin-induced cytotoxicity. In the protective effect of Lemon balm extract on cisplatin-induced cytotoxicity, it significantly increased cell viability which was decreased by cisplatin-induced cytotoxicity, and also it showed the DPPH-radical scavenging activity and LDH inhibitory activity. Therefore, these results suggested that the cytotoxicity of cisplatin may be involved in oxidative stress, and also, Lemon balm extract effectively prevented the cytotoxicity induced by cisplatin via antioxidative effect. Conclusively, the natural extract like Lemon balm may be useful for the development as antioxidative agent via the prevention of the cytotoxicity induced by pharmaceutic alopesia inducer correlated with oxidative stress.

보문 : 피부염 유발제인 삼산화크롬(CrO3)으로 손상된 배양 NIH3T3 섬유모세포에 대한 조개나물 추출물의 항산화 효과

정재윤 ( Jai Yun Jung ),오성균 ( Seung Kyun Oh ),박신희 ( Shin Hee Park ),윤미영 ( Mi Young Yoon ),유영월 ( Yeong Wol Yu ),임요섭 ( Yo Sup Rim ),정인주 ( In Ju Jung ) 대한미용학회(구 대한미용과학회) 2014 대한미용학회지 Vol.10 No.1

This study was to evaluate the protective effect of Ajuga multiflora BUNGE(AMB) extract on the cytotoxicity induced by chromium trioxide (CrO3), dermatitis inducer. For this study, the cytotoxicity induced by CrO3 was assessed by colorimetric assay after NIH3T3 fibroblasts were cultured in media containing various concentrations of CrO3. And also, the effect of vitamin E was assessed on the CrO3-induced cytotoxicity. For the protective effect of AMB extract on CrO3- induced cytotoxicity, NIH3T3 fibroblasts were pretreated with 60 or 80 μg/mL of AMB extract for 2 hours, and also, antioxidative effects of ABM extract were analysed via electron donating ability (EDA) and lactate dehydrogenase (LDH) activity. In this study, CrO3 significantly decreased cell viability in a dose-dependent manner, and the XTT50 value was calculated at 28.4 μM. In the effect of vitamin E, it significantly increased cell viability decreased by CrO3-induced cytotoxicity. In the protective effect of AMB extract on CrO3-induced cytotoxicity, it significantly increased cell viability which was decreased by CrO3-induced cytotoxicity, and also it showed EDA and LDH inhibitory activity. From these results, it is suggested that the cytotoxicity of CrO3 is involved in oxidative stress, and also, AMB extract effectively prevented CrO3-induced cytotoxicity by the antioxidative effect. Conclusively, the natural plant component like AMB may be a putative agent for the prevention of the cytotoxicity induced by dermatitis inducer correlated with oxidative stress.