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치매유발제인 알루미늄으로 손상된 배양 C6 Glioma 세포에 대한 금은화 추출물의 보호 효과
정재윤,정인주,제갈승주,Jung, Jai-Yun,Jung, In-Ju,Jekal, Seung-Joo 대한임상검사과학회 2017 대한임상검사과학회지(KJCLS) Vol.49 No.3
This study was performed to evaluate the cytotoxicity of aluminum chloride ($AlCl_3$), and the protective effect of Lonicerae flos (LF) extract on $AlCl_3-induced$ cytotoxicity in the cultured C6 glioma cells. Here, the cell viability and antioxidative effects, such as DPPH-radical scavenging activity, superoxide anion scavenging activity, and lipid peroxidation (LP), were assessed. $AlCl_3$ significantly decreased the cell viability in a dose-dependent manner; the $XTT_{50}$ value was measured at $128.8{\mu}M$ of $AlCl_3$. The cytotoxicity of $AlCl_3$ was determined as highly toxic the y Borenfreund and Puerner's toxic criteria. The butylated hydroxytoluene (BHT) and antioxidant both significantly increased the cell viability, which was damaged by $AlCl_3-induced$ cytotoxicity in these cultures. In the protective effect of LF extract on $AlCl_3-induced$ cytotoxicity, the LF extract significantly increased the superoxide anion scavenging activity and inhibitory activity of LP, as well as the DPPH-radical scavenging activity. From these results, it is suggested that the oxidative stress may have been involved in the cytotoxicity of $AlCl_3$, and LP extract effectively protected $AlCl_3-induced$ cytotoxicity through the antioxidative effects. Conclusively, the natural resources, like LP extract, may be a putative therapeutic agent for the treatment of dementia induced by allergen like aluminum correlated with the oxidative stress.
보문 : 염모제 성분인 FeSO4의 세포독성에 대한 산사 추출물의 항산화 효과
정재윤 ( Jai Yun Jung ),서영미 ( Young Mi Seo ),정인주 ( In Ju Jung ) 대한미용학회(구 대한미용과학회) 2013 대한미용학회지 Vol.9 No.4
To clarify the antioxidative effect of Crataegi Fructus (CF) extract on the cytotoxicity induced by FeSO4, hair dye component, antioxidative effects such as DPPH-radical scavenging activity and lactate dehydrogenase (LDH) activity as well as cell viability by XTT assay were performed. Cell viability and LDH activity were assessed after NIH3T3 fibroblasts were cultured in media containing various concentrations of FeSO4. And also, the effect of antioxidant, vitamin E on FeSO4-induced cytotoxicity was evaluated. For the protective effect of CF extract on FeSO4-induced cytotoxicity, cell viability was measured after NIH3T3 fibroblasts were pretreated with 130 or 160 μg/mL of CF extract for 2 hours, and also, DPPH-radical scavenging activity and the inhibitory activity of LDH were assessed on CF extract. In this study, FeSO4 significantly decreased cell viability in dose-dependently compared with control, and XTT50 value was calculated at 33.4 μM of FeSO4. In the protective effect of vitamin E, it significantly increased cell viability damaged by FeSO4-induced cytotoxicity. In the protective effect of CF extract on FeSO4-induced cytotoxicity, CF extract significantly increased cell viability which was decreased by FeSO4, and also it showed DPPH-radical scavenging activity and the inhibitory effect of LDH activity. From these results, it is suggested that the oxidative stress is involved in the cytotoxicity of FeSO4, and also, CF extract effectively prevented FeSO4-induced cytotoxicity by antioxidative effect. Conclusively, the natural plant extract such as CF may be a putative resources for beauty by the prevention or diminution of the cytotoxicity of hair dye component such as FeSO4 correlated with oxidative stress.
보문 : 피부염 유발제인 삼산화크롬(CrO3)으로 손상된 배양 NIH3T3 섬유모세포에 대한 조개나물 추출물의 항산화 효과
정재윤 ( Jai Yun Jung ),오성균 ( Seung Kyun Oh ),박신희 ( Shin Hee Park ),윤미영 ( Mi Young Yoon ),유영월 ( Yeong Wol Yu ),임요섭 ( Yo Sup Rim ),정인주 ( In Ju Jung ) 대한미용학회(구 대한미용과학회) 2014 대한미용학회지 Vol.10 No.1
This study was to evaluate the protective effect of Ajuga multiflora BUNGE(AMB) extract on the cytotoxicity induced by chromium trioxide (CrO3), dermatitis inducer. For this study, the cytotoxicity induced by CrO3 was assessed by colorimetric assay after NIH3T3 fibroblasts were cultured in media containing various concentrations of CrO3. And also, the effect of vitamin E was assessed on the CrO3-induced cytotoxicity. For the protective effect of AMB extract on CrO3- induced cytotoxicity, NIH3T3 fibroblasts were pretreated with 60 or 80 μg/mL of AMB extract for 2 hours, and also, antioxidative effects of ABM extract were analysed via electron donating ability (EDA) and lactate dehydrogenase (LDH) activity. In this study, CrO3 significantly decreased cell viability in a dose-dependent manner, and the XTT50 value was calculated at 28.4 μM. In the effect of vitamin E, it significantly increased cell viability decreased by CrO3-induced cytotoxicity. In the protective effect of AMB extract on CrO3-induced cytotoxicity, it significantly increased cell viability which was decreased by CrO3-induced cytotoxicity, and also it showed EDA and LDH inhibitory activity. From these results, it is suggested that the cytotoxicity of CrO3 is involved in oxidative stress, and also, AMB extract effectively prevented CrO3-induced cytotoxicity by the antioxidative effect. Conclusively, the natural plant component like AMB may be a putative agent for the prevention of the cytotoxicity induced by dermatitis inducer correlated with oxidative stress.
보문 : 모발염색제 성분인 초산납에 대한 어성초 추출물의 항산화 및 미백효과
정재윤 ( Jai Yun Jung ),오영희 ( Young Hee Oh ),박신희 ( Shin Hee Park ),윤미영 ( Mi Young Yoon ),표애자 ( Ae Ja Pyo ),김선주 ( Sun Ju Kim ),유영월 ( Yeong Wol Yu ),정인주 ( In Ju Jung ) 대한미용학회(구 대한미용과학회) 2014 대한미용학회지 Vol.10 No.2
To evaluate the antioxidative and whitening effects of Houttuynia cordata Thunb (HCT) extract on lead acetate (LA), hair dye component, cell viability was measured by colorimetric assay after NIH3T3 fibroblasts were cultured in media containing various concentrations of LA. And also, the effect of antioxidant, vitamin E on LA-induced cytotoxicity was analysed. For the protective effect of HCT extract on LA-induced cytotoxicity, NIH3T3 fibroblasts were pretreated with 110 or 130 μg/mL of HCT extract for 2 h before the treatment of LA. And also, the antioxidative and melanogenic effects of HCT extract were assessed by superoxide dismutase (SOD)-like activity, total amount of melanin and tyrosinase activity. In this study, LA significantly decreased cell viability in dose-dependent manner. And the XTT50 value was calculated at 48.3 μM of LA. In the effect of antioxidant, vitamin E, it effectively prevented LA-induced cytotoxicity by the significant increase of cell viability. In the protective effect of HCT extract on LA-induced cytotoxicity, HCT extract significantly increased cell viability which was decreased by the cytotoxicity induced by LA, and also it showed the antioxidative effect by SOD-like activity. In the melanogenesis, HCT extract showed the decreased of amount of melanin and tyrosinase activity. From these results, it is suggested that the cytotoxicity of LA is involved in oxidative stress, and HCT extract effectively prevented the cytotoxicity and the melanogenesis induced by LA.