바이러스 백신 중 위해 HBV 바이러스에 대한 효율성이 높은 검색기술 개발

심정현(Jung-Hyun Shim),이경애(Kyung-Ae Lee),김종완(Jong Wan Kim),남승우(Seung Woo Nam),신진호(Jin-ho Shin),박순희(Sue-Nie Park),조용권(Yong-Kweon Cho),윤도영(Do-Young Yoon) 대한미생물학회 2003 Journal of Bacteriology and Virology Vol.33 No.1

웨어러블 컴퓨터를 위한 인터페이스 디자인 유형 개발에 관한 연구 : 의복의 메타포 활용을 중심으로

심정현(Shim jung hyun),정지홍(Jung ji-hong) 한국HCI학회 2004 한국HCI학회 학술대회 Vol.2004 No.2

다중파일 전송방식 기반의 P2P프로그램에서 데이터 전송상황의 시각화에 관한 연구

심정현(Shim Jung-Hyun),정지홍(Jung Ji-Hong) 한국디자인학회 2003 한국디자인학회 학술발표대회 논문집 Vol.- No.-

수용성 β-1,3-glucans의 면역 활성 효능에 대한 연구

심정현,최원아,김종완,이해숙,백태웅,조민철,이경애,상병찬,윤도영,Shim, Jung-Hyun,Choi, Won-A,Kim, Jong-Wan,Lee, Hae-Sook,Baek, Tae-Woong,Cho, Min-Cheol,Lee, Kyung-Ae,Sang, Byung-Chan,Yoon, Do-Young 대한면역학회 2003 Immune Network Vol.3 No.2

Background: $\beta$-1,3-glucans are well known to enhance the immune reactions, resulting in antitumor, antibacterial, antiviral, anticoagulatory and wound healing activities. $\beta$-1, 3-glucans have various activities depending on molecular weight, degree of branching, conformation, water-solubility and intermolecular association. However, the $\beta$-1,3-glucan linked backbone structure is essential and $\beta$-D-glucopyranosyl units are required for immuno-potentiating activities. Result: In this study, we tested the immunophamacological activities of soluble $\beta$-1,3-glucans and confirmed the following activities: (1) $IFN-{\gamma}$ production in PBMCs in the presence or the absence of PHA, LPS, or IL-18; (2) induction of various cytokines in the spleen and thymus; (3) adjuvant effect on the antibody production; (4) nitrogen oxide synthesis in macrophages; (5) the cytotoxic and antitumor effects on cell lines and ICR mice. Conclusion: These results strongly suggested that $\beta$-1,3-glucans possessed various immuno-pharmacological activities.

나선형 분지 스텐트 내에서의 혈류 유동 수치모사

심정현(Jeong Hyun Shim),이경은(Kyung Eun Lee),유정열(Jung Yul Yoo) 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.5

It is broadly accepted that bifurcated stent-graft signifies an improvement in surgical technique for treatment of a lesion in the branched blood vessel. However, there still remains the high failure rate regarding bifurcated stent-graft due to the occurrence of thrombosis or re-stenosis. The objectives of this study are to understand the effect of torsion in helical bifurcated geometries, to explain how the mixing of flows in the geometries may be advantageous to the prevention of the occurrence of thrombosis, and to keep the patency of stent-graft in the aspect of hemodynamics. For clinical applications, flows in a helical bifurcated stent-graft with a small helix amplitude are simulated three-dimensionally using a Navier-Stokes solver. In this study, the hemodynamics is investigated in terms of mechanical factors, i.e., velocity profiles, vortex patterns and wall shear stress distributions. The result of this study can be utilized as design information for the developed devices.

Agrobacterium sp. R259 KCTC 10197BP로 부터 생산된 β-1, 3-glucan 의 면역 활성 효능

심정현(Jung Hyun Shim),최원아(Won-A Choi),상병찬(Byung Chan Sang),윤도영(Do Young Yoon) 대한약학회 2002 약학회지 Vol.46 No.6

β-1, 3-glucans are well known to enhance the immune reactions, resulting in antitumor antibacterial, antiviral, anticoagulatory and wound healing activities. Β-1, 3- glucans have various activities depending on molecular weight, degree of branching, conformation, water-solubility and intermolecular association. However the β-1,3- glucans linked backbone structure is essential and β-D-glucopranosyl units are required for immunopotentiating activities. In this stud7; we tested the immunophamacological activities of insoluble β-1,3-glucan from Agrobacterium sp. R259 KCTC 10197BP and confirmed the following activities : (1) IFN-γproduction in PBMCS in the presence or in the absence of PHA, LPS, IL-18, and IL-12; (2) the induction of various cytokines in the spleen and thymus; (3) the adjuvant effect on the antibody production; (4) the cytotoxic and antitumor effects on cell lines and ICR mice. These results strongly suggest that β-1,3-glucan from Agrobacterium sp. R259 KCTC 10197BP possesses various immunopharmacological activities.

Proteomics-based Identification of Components in the Adventitious Roots of Panax Ginseng C. A. Mayer related to Energy Metabolism and Antibiotic Effects

조진형,전영주,이라함,심정현,채정일,Cho, Jin-Hyoung,Jeon, Young-Joo,Lee, Ra-Ham,Shim, Jung-Hyun,Chae, Jung-Il Korean Association of Organic Agriculture 2014 韓國有機農業學會誌 Vol.22 No.1

Korean Panax ginseng C. A. Meyer (P. ginseng) is a well-known and one of the most important tonic herbs used in traditional Korean medicine. The pharmacological effects of P. ginseng have been reported by many researchers. Nevertheless, little is known between the mechanism of action and the active compounds. In this study, we performed a comprehensive proteomic analysis and protein categorization in order to understand the physiological characteristics of the major components in the adventitious roots of P. ginseng. Whole proteins extracted from the cultured adventitious roots of P. ginseng were separated by two-dimensional polyacrylamide gel electrophoresis (2-DE). Among the 1000 spots which were detected by silver staining, 113 spots were labeled and identified by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF MS). Our results showed that 40 proteins were identified among the 113 spots, with a hit ratio of 35.3%. A number of proteins identified on the 2-DE gels (30%; 16 spots) were involved in energy metabolism. These proteomic data will be helpful to better understand the physiological and pharmacological effects of P. ginseng.

동결견의 진단과 치료 경과 판정에 대한 초음파 검사의 유용성

최창혁,김석준,이상화,김희수,심정현,Choi, Chang-Hyuk,Kim, Seok-Jun,Lee, Sang-Hwa,Kim, Hee-Soo,Shim, Jung-Hyun 대한정형외과초음파학회 2014 대한정형외과 초음파학회지 Vol.7 No.1

목적: 동결견 환자에서 초음파를 통한 검사가 진단 및 치료 후 예후 판단에 객관적 자료로 쓰일 수 있는지 확인하고자, 진단 시 및 스테로이드 주사 치료 후 반대측과 비교 관찰하였다. 대상 및 방법: 저자들은 2012년 7월에서 2013년 7월 사이, line 1(CBT: 오구돌기-이두건)과 line 2(CBG: 오구돌기-이두건 고랑)를 이용한 초음파 검사를 통한 진단을 시행하였다. 대상이 된 20명의 환자에 대해 스테로이드 1회 주사 치료후 그 결과를 2, 4, 6개월의 추시 관찰을 통하여 초음파 연속 검사 상 참고선의 호전 정도 및 견관절 운동 범위 회복 정도를 비교하였다. 결과: 초음파 검사상 환측과 건측의 차이는 치료 전 CBT이 중립, 외회전, 내회전에서 각각 -5.6 mm, -5.0 mm, -4.3 mm였으며 CBG는 -6.1 mm, -4.7 mm, -5.0 mm이었으며(p<0.05), 2개월(CBT: -4.8 mm, -3.5 mm, -2.6 mm/CBG: -4.7 mm, -4.0 mm, -3.6 mm), 4개월(CBT: -4.7 mm, -3.2 mm, -1.7 mm/CBG: -4.3 mm, -3.7 mm, -1.2 mm), 6개월(CBT: -1.1 mm, -2.9 mm, -0.5 mm / CBG: -1.2 mm, -0.7 mm, -0.9 mm)로써 내회전은 4개월, 중립 및 외회전은 6개월 이후 호전되는 소견을 보였다(p<0.05). 운동 범위는 치료 전 전방거상, 외회전, 내회전이 108도, 32도, L5, 2개월에는 117도, 35도, L4, 4개월에는 133도, 42도, L3, 6개월에는 136도, 47도, L2로써 4개월 이후 유의한 회복소견을 보였다. 시각통증등급(VAS)은 치료전 7.5에서 2개월에는 3.0으로 유의한 호전을 보였다(p<0.05). 결론: 스테로이드 주사 치료 후 참고선은 6개월 후 회복되는 소견을 보여, 운동 범위는 4개월 후, 통증은 2개월 후 호전을 보인 것에 비해 다소 지연되어 나타나는 경향이었다. 동적 초음파 검사는 동결견 환자에서 진단 및 예후 판단 시 유효한 검사 방법으로 이용할 수 있을 것으로 생각되었다. Purpose: In order to know the effectiveness of ultrasonographic examination in diagnosis and follow-up evaluation for the frozen shoulder, we compared those with contralateral side in initial and after steroid injection. Materials and Methods: For the 20 unilateral frozen shoulder from July 2012 to July 2013, ultrasonographic examination was tried using reference line 1 (CBT: distance from coracoid process to LHB tendon) and line 2 (CBG: distance from coracoid process to bicipital groove). We tried 1 time steroid injection and compared the improvement in gross motion and reference line with 2 month, 4 month, and 6 month's serial ultrasonographic examination. Results: The differences on CBT line between lesion side and normal side were -5.6 mm, -5.0 mm, and -4.3 mm in neutral (Neut), external rotation (ER) and internal rotation (IR), respectively. The differences on CBG line were -6.1 mm, -4.7 mm, and -5.0 mm respectively (p<0.05). The changes in the reference line after steroid injection were evaluated at 2 month (CBT: -4.8 mm, -3.5 mm, -2.6 mm / CBG: -4.7 mm, -4.0 mm, -3.6 mm), 4 month (CBT: -4.7 mm, -3.2 mm, -1.7 mm / CBG: -4.3 mm, -3.7 mm, -1.2 mm), and 6 month (CBT: -1.1 mm, -2.9 mm, -0.5 mm / CBG: -1.2 mm, -0.7 mm, -0.9 mm). The gross motion was improved at 4 month after injection, from elevation $108^{\circ}$, ER $32^{\circ}$, IR L5 in initial to $133^{\circ}$, $42^{\circ}$, L3 respectively (p<0.05). Pain improved from 7.5 in initial to 3.0 at 2 month on visual analog scale (VAS). Conclusion: The serial examination after steroid injection revealed that the delayed improvement on reference line (6 month) compared with pain (2 month) or gross motion (4 month). Dynamic ultrasonogram was useful in diagnosis and follow-up evaluation of frozen shoulder.

토양 균주 발효 추출물 Nargenicin 및 그 유도체의 항생제 대체 효과능 평가

조승식,홍준희,채정일,심정현,나종삼,유진철,Cho, Seung-Sik,Hong, Joon-Hee,Chae, Jung-Il,Shim, Jung-Hyun,Na, Chong-Sam,Yoo, Jin-Cheol 한국유기농업학회 2014 韓國有機農業學會誌 Vol.22 No.3

본 연구진은 신규 미생물인 Nocardia sp. CS682 균주에서 항균물질인 nargenicin을 확보하고, 그 유도체 5종을 확보하여 그람양성, 음성 및 다약제 내성균에 대한 항균 효능 및 LPS로 자극된 대식세포에서의 nitric oxide 생성 억제능을 확인하였다. Nargenicin 유도체들은 nargenicin 및 vancomycin에 비교하여 우수한 항균 활성을 보여주었으며, compound 4 및 5는 광범위한 항균 효능 외에 nitric oxide 생성 억제능을 보여 항균-항염효과를 가지는 dual effector로써 감염, 면역 질환에 응용 가능성을 시사하였다. Nargenicin 유도체들은 향후 염증반응에서의 면역 조절 기작에 대한 추가 연구가 필요할 것으로 사료된다. IIn vitro antimicrobial and anti-inflammatory activities of nargenicin and its derivatives were investigated. Nargenicin, an unusual macrolide antibiotic with potent anti-MRSA (methicilin-resistant Staphylococcus aureus) activity, was purified from the culture broth of Nocardia sp. CS682. And variety of novel nargenicin derivatives was synthesized from nargenicin. Two compounds (4 and 5) exhibit a broad spectrum of antimicrobial activities against infectious bacteria. The antimicrobial activity of derivatives against fifteen organisms was assessed using the minimum inhibitory concentration (MIC). The MIC values were in the ranges of $0.15{\sim}80{\mu}g/mL$ (w/v) for compound 1 and 2, $5{\sim}80{\mu}g/mL$ (w/v) for compound 3, $1.25{\sim}40{\mu}g/mL$ (w/v) for compound 4, and $1.25{\sim}80{\mu}g/mL$ (w/v) for compound 5, depending on the pathogens studied. In vitro, we investigated cytotoxicity and inhibition of nitric oxide (NO) production of synthesized compounds 1-5 in Raw 264.7 cells. LPS-induced nitric oxide releases were significantly blocked by compound 3, 4 and 5 in a dose-dependent manner. At high concentrations ($5{\mu}g/mL$) compound 5 inhibited the NO production by 95%. Compound 4 inhibited the release of NO in LPS-activated Raw 264.7 cells by 75% at the concentration of $10{\mu}g/mL$. Compound 3 inhibited the release of NO in LPS-activated Raw 264.7 cells by 65% at the concentration of $100{\mu}g/mL$. On the other hand, nargenicin, compound 1 and 2 did not inhibit NO production. These results demonstrated that compound 4 and 5 displayed antimicrobial activity and blocked LPS-induced pro-inflammatory mediators such as NO in macrophages, which might be responsible for its therapeutic application.

클라우드 컴퓨팅 환경에서의 프로젝트 수행 성과에 관한 연구

우혁준(Hyeok-Jun Woo),심정현(Jeong-Hyun Shim),이정훈(Jung-Hoon Lee) 한국전자거래학회 2011 한국전자거래학회지 Vol.16 No.1

IT 기술의 발전으로 시간적, 물리적 제약을 넘은 미래 인터넷 기술에 대한 사용자들의 기대가 커지고 있다. 이러한 요구를 충족시키기 위해 최근 새로운 개념으로 주목 받고 있는 것이 클라우드 컴퓨팅이다. 그러나 이러한 긍정적인 전망에도 불구하고 클라우드 컴퓨팅의 도입은 활발히 이루어지고 있지 않고 있는 실정이다. 따라서 본 연구에서는 클라우드 컴퓨팅 환경의 특성이 프로젝트 수행성과에 어떠한 영향을 미치는가에 대하여 살펴보았다. 본 연구는 기술적 도구로써 클라우드 컴퓨팅을 과업과 기술의 적합성 모형에 적용하여 클라우드 컴퓨팅 환경에서 개인이 프로젝트를 수행할 시에 나타나는 성과에 대하여 실증적인 연구를 하였다. 대학교 및 대학원에서 팀 단위의 프로젝트를 수행할 때, 클라우드 컴퓨팅의 가장 널리 보급된 형태인 GoogleDocs 및 웹하드에 대한 사용경험이 있는 응답자를 대상으로 설문을 수행하였다. 본 연구에서는 클라우드 컴퓨팅 환경에서 프로젝트를 수행한다는 행위에 중점을 두어, 접근성과 신뢰성을 적합성을 파악하기 위한 1차 요인으로 정의하여 프로젝트의 상호의존성과 개인의 특성이 적합성 및 활용을 통하여 성과에 어떤 영향을 미치는지를 분석하였다. 연구결과, 클라우드 컴퓨팅 환경은 팀 단위의 프로젝트 수행에 있어 적합한 도구라는 사실을 규명하였다. 본 연구는 초기단계의 클라우드 컴퓨팅에 있어 개인이 지각하는 적합성 및 성과를 규명함으로써, 협업에서의 클라우드 컴퓨팅의 긍정적인 영향에 대한 근거를 제시한다. There are expectations about future internet technology with IT development by end-users. Cloud computing is attracted to satisfy those demands. However, adoption of cloud computing is not active that much. Therefore, this study verified how cloud computing environment affects performance of team project. We conducted empirical study on performance of team project with cloud computing as technology tool focusing on Task-Technology Fit Model. We collected samples that were undergraduate and graduate school students and had experience on initial cloud computing such as Google-Docs and Webhard when they conducted team project for assignment. We focused on accessibility and reliability as task-technology fit and those variables treated as first order factor. Result showed that cloud computing is suitable technology tool for team project. This study suggests positive effects of cloud computing for collaboration by proving perceived fit and performance in initial cloud computing.