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유경단(Gyeong-Dan Yu),장윤희(Yun-Hui Jang),김정일(Jeong-Il Kim),이지은(Ji-Eun Lee),안기홍(Gi Hong An),문윤호(Youn-Ho Moon),차영록(Young-Lok Cha),최인후(In-Hu Choi),안종웅(Jong Woong Ahn),구본철(Bon-Cheol Koo),이경보(Kyung-Bo Lee) 한국육종학회 2015 한국육종학회지 Vol.47 No.4
This study was carried out to establish the optimal conditions for callus induction and plant regeneration using immature inflorescence of M. sacchariflorus cv. ‘Wooram’, a bioenergy crop selected in Korea. Callus induction rate was the highest (93.3%) in MS medium containing 3 mg L-1 2,4-D, and 86.7% in MS medium containing 3 mg L-1 2,4-D combined with 0.1 mg L-1 BA. Plant regeneration rate was high when the calli derived from the medium containing BA was used, as compared with those derived from the BA-minus medium. The results showed that the medium conditions containing 5 mg L-1 BA combined with 0.1 mg L-1 NAA was the most effective in plant regeneration of which the rate reached 86.7%. The regenerated shoots were separated from the calli and roots over 3 cm were developed from the shoots after 4 week culture on basal MS medium without supplementation. The plantlets were then transferred to soil and cultured in greenhouse. After 5 weeks, the plants with the height of at least 20 cm were successfully acclimatized.
Voriconazole Therapeutic Drug Monitoring is Necessary for Children with Invasive Fungal Infection
강현미,강수영,조은영,유경상,이지원,강형진,박경덕,신희영,안효섭,이현주,최은화,이환종,Kang, Hyun Mi,Kang, Soo Young,Cho, Eun Young,Yu, Kyung-Sang,Lee, Ji Won,Kang, Hyoung Jin,Park, Kyung Duk,Shin, Hee Young,Ahn, Hyo Seop,Lee, Hyunju,Choi, The Korean Society of Pediatric Infectious Disease 2014 Pediatric Infection and Vaccine Vol.21 No.1
목적: 본 연구는 소아 환자들에서 voriconazole 치료적 약물 농도 모니터링의 임상적 의의를 분석하고자 하였다. 방법: 2010년 7월부터 2012년 6월까지 서울대학교병원에 입원한 18세 이하의 소아 환자들 중, 침습성 진균감염증에 대해 voriconazole 치료를 받은 증례를 후향적 의무기록 분석을 통해 분석하였다. 본 연구에 포함된 총 28명의 환자 중 14명이 약물 농도 모니터링을 받았으며, 143개의 혈중 농도 측정 값을 분석하였다. 모든 환자들에게서 치료 효과 및 독성 증상 발현 여부를 파악하였다. 결과: 143개의 혈중 농도 측정 값 중 53.1%에서 치료적 범위(1.0-5.5 mg/L) 내에 들었고, 같은 용법으로 치료받았더라도 높은 혈중 농도 변동성(high variability)을 보였다. 약물 농도 모니터링을 받았던 군(TDM 군)과 받지 않았던 군(non-TDM 군)에서 각각 14명 중 9명(64.3%)이 독성 증상을 나타냈는데, TDM 군에서 신경학적 증상(n=2, 14.3%) 및 간기능 장애(n=8, 57.1%)는 높은 voriconazole 혈중 농도(>5.5 mg/L)를 보인 환자들에게서 나타났다. 반면, 시각 장애는 혈중 농도가 치료적 범위 내에 있을 때 발현하였다(1.18 mg/L, 3.9 mg/L). TDM 군에서 non-TDM 군에 비하여 독성 증상으로 인하여 약물을 중단했던 빈도가 낮았다(0.0% vs. 18.2%, P =0.481). 치료 시작 6주 후 치료 효과를 분석해본 결과 TDM 군의 57.2%에서 치료에 대한 반응을 보였으나, non-TDM 군에서는 14.3%에서 치료 반응을 보였다(P =0.055). 최종 치료효과 분석에서는 TDM 군의 21.4%에서 치료 반응을 보였으나, non-TDM 군의 14.3%에서 치료 반응을 보였다(P =0.664). TDM 군에서 치료 시작 첫 6주 동안 혈중 약물 농도를 분석했을 때 67.0% 이상에서 치료적 범위 내에 들었으나, 치료 기간 전체를 봤을 때에는 45.5%에서 치료적 범위 내에 들었다. 결론: 소아에서 voriconazole 사용 시 치료적 약물 농도 모니터링을 통하여 치료 목표를 효과적으로 달성하고, 독성이 나타나는 것을 예방할 수 있다. Purpose: To determine the clinical significance of voriconazole therapeutic drug monitoring (TDM) in the pediatric population. Methods: Twenty-eight patients with invasive fungal infections administered with voriconazole from July 2010 to June 2012 were investigated retrospectively. Fourteen received TDM, and 143 trough concentrations were analyzed. All 28 patients were assessed for adverse events and treatment response six weeks into treatment, and at the end. Results: Out of 143 samples, 53.1% were within therapeutic range (1.0-5.5 mg/L). Patients administered with the same loading (6 mg/kg/dose) and maintenance (4 mg/kg/dose) dosages prior to initial TDM showed highly variable drug levels. Adverse events occurred in 9 of 14 patients (64.3%) in both the TDM and non-TDM group. In the TDM group, voriconazole-related encephalopathy (n=2, 14.3%) and aspartate aminotransferase (AST) or alanine aminotransferase (ALT) elevation (n=8, 57.1 %) occurred with serum levels in the toxic range (>5.5 mg/L), whereas blurred-vision (n=2, 14.3%) occurred within the therapeutic range (1.18 mg/L and 3.9 mg/L). The frequency of voriconazole discontinuation due to adverse events was lower in the TDM group (0.0% vs. 18.2%, P =0.481). Overall, 57.2% of the patients in the TDM group versus 14.3% in the non-TDM group showed clinical response after 6 weeks (P =0.055), whereas 21.4% in the TDM group versus 14.3% in the non-TDM group showed response at final outcome (P =0.664). In the TDM group, >67.0% of the serum levels were within therapeutic range for the first 6 weeks; however 45.5% were within therapeutic range for the entire duration. Conclusion: Routine TDM is recommended for optimizing the therapeutic effects of voriconazole.
안재민 ( Jae-min An ),홍경숙 ( Kyong-suk Hong ),윤혜정 ( Hye-jeong Yun ),김성연 ( Sung-youn Kim ),유경은 ( Kyung-eun Yu ),신희창 ( Hee-chang Shin ),윤재돈 ( Jae-don Yun ),이재훤 ( Jae-hwon Lee ),김동호 ( Dong-ho Kim ) 한국환경농학회 2016 한국환경농학회 학술대회집 Vol.2016 No.-
The present study was carried out to reduction of lead(Pb), cadmium(Cd), mercury(Hg), arsenic(As), chromium(Cr) and nickel(Ni) concentrations by 5 milling degrees(0.0%, 2.4%, 4.8%, 7.2% and 9.6%, from husked rice to polished rice) in rice. Recently, various rice by milling degree is sold for health and taste. To provide safe food to consumers, it is need to know the change of their concentrations according to milling degree of rice. Pb, Cd, As, Cr and Ni analysis in milled rice 255 samples were performed using microwave device and Inductively Coupled Plasma Mass Spectrometry(ICP/MS), whereas mercury was determined by automatic mercury analyzer(DMA-80). The contents of lead, cadmium, mercury, arsenic, chromium and nickel in samples were determined. The contents of lead was 0.0010-0.0572 mg/kg, cadmium was 0.0031-0.1009 mg/kg, mercury was 0.0001-0.0421 mg/kg, arsenic was 0.028-0.244 mg/kg, chromium was 0.016-0.495 mg/kg and nickel was 0.027-0.608 mg/kg, respectively. The Limit of detection(LOD), Limit of quantification(LOQ) and correlation coefficient(R2) for heavy metals(Pb, Cd, Hg, As, Cr and Ni) were 0.38-0.66 μg/kg, 1.21-2.09 μg/kg and 0.9999-1.0000, respectively. The recovery results and coefficient of variations were in the range 91.3-103.5% and 2.82-4.50%, by tested certified reference materials(CRM) and spiked standard solutions. For risk assessment, daily intakes of heavy metals were calculated and compared with provisional tolerable weekly intake(PTWI) or provisional tolerable monthly intake(PTMI) established by the Joint FAO/WHO Expert Committee on Food Additives(JECFA). Therefore, the level of overall dietary exposure to heavy metals for Korean through from these results, conforming with increase of milling degree of rice, heavy metal concentration tended to decrease. The contents of heavy metal were decreased 57.7% in lead, 13.0% in cadmium, 50.3% in mercury, 38.2% in arsenic, 59.1% in chromium and 48.5% in nickel in according to the highest milling degree of 9.6%(polished rice). The reduction rate of heavy metals were as Cr>Pb>Hg>Ni>As>Cd.
소각로 인근에서 재배되는 농산물의 다이옥신류 모니터링 및 위해도 평가에 관한 연구
신희창 ( Hee-chang Shin ),홍경숙 ( Kyong-suk Hong ),안재민 ( Jae-min An ),윤혜정 ( Hye-jeong Yun ),김성연 ( Sung-youn Kim ),유경은 ( Kyung-eun Yu ),윤재돈 ( Jae-don Yun ),이재훤 ( Jae-hwon Lee ),김동호 ( Dong-ho Kim ) 한국환경농학회 2016 한국환경농학회 학술대회집 Vol.2016 No.-
Dioxins(PCDDs; Polychlorinated dibenzo-p-dioxins, PCDFs; Polychlorinated dibenzofurans) are accumulated in the body through the food as a representative environmental pollutants. Dioxins are flowed into the environment through the incineration of chemical products and electrical products containing PCBs. Dioxins generated from incinerators can pollute the atmosphere and soil, and it were accumulated in the body through food. Therefore, the dioxins monitoring of agricultural products produced near incinerators are required to ensure the safety of agricultural products. In this study, the analysis of dioxins was carried out on the basis of the law of the U.S Environment Protection Agency (EPA) Method 1613 and Korean Food Standards Codex Method SOP. The fifty agricultural products were collected in the field around the incinerators. As a result, the concentration range of detected dioxins was (0.0507~0.5824) pg/g. In the range of concentrations that reflect the toxic equivalent factor is (0.00002~0.01631) TEQ pg/g. The contribution of agricultural products to the dioxins intake is not equally distributed: tomato (74%), Corn (15%), Cabbage (9%), Green pepper (2%), red pepper (0.04%), Blueberries (0.0006%). The estimated daily intake of Korean Population through agricultural products was 0.00430 pg TEQ/kg body weight/day, and it was estimated at about 0.11% of tolerable daily intake(TDI) of Korea. Consequently, Dioxins exposure levels of agricultural products produced near incinerators has been found to be very safe.
Yu Kyung Park(박유경),Chang-Eun Lee(이창은),Hyoungseok Lee(이형석),Hye Yeon Koh(고혜연),Sojin Kim(김소진),Sung Gu Lee(이성구),Jung Eun Kim(김정은),Joung Han Yim(임정한),Ju-Mi Hong(홍주미),Ryeo-Ok Kim(김려옥),Se Jong Han(한세종),Il-Ch 한국생명과학회 2020 생명과학회지 Vol.30 No.11
TCTP는 다양한 진핵생물에서 풍부하게 존재하는 단백질 중에 하나이며, 암, 세포 증식, 유전자 조절 등과 관련된 세포의 생리학적 기작에서 중요한 역할을 담당하는 것으로 알려져 왔다. 더구나, TCTP는 dithiothreitol (DTT)나 hydrogen peroxide (H₂O₂)에 의해 유도되는 산화적 스트레스에 대한 저항성에 관여하는 중요한 단백질로 주목받아 왔다. 한편, 극지역 서식 생물들은 강한 자외선에 의해 발생한 활성산소를 조절하기 위한 다양한 항산화 방어체계를 가지고 있다. 본 연구에서는 북극 동물플랑크톤 Calanus glacialis에서 분리된 TCTP가 산화적 스트레스 하에서 E. coli 세포의 저항성에 미치는 효과를 관찰하였다. C. glacialis에서 분리된 TCTP 유전자(ORF 522 bp) 서열을 분석하였고, 약 23 kDa의 재조합 단백질을 제작하였다. 관찰 결과, TCTP 재조합 단백질이 E. coli 세포에서 과발현되었을 때, 세포들은 H₂O₂에 의해 유도된 산화적 스트레스에 대한 저항성이 증가하는 것을 확인하였다. 본 관찰을 통해, 북극 C. glacialis TCTP 단백질의 항산화 조절자로서의 역할에 대한 가능성을 처음으로 제시하였다. Translationally controlled tumor protein (TCTP) is one of the most abundant proteins in various eukaryotic organisms. TCTPs play important roles in cell physiological processes in cancer, cell proliferation, gene regulation, and heat shock response. TCTP is also considered an important factor in the resistance to oxidative stress induced by dithiothreitol or hydrogen peroxide (H₂O₂). Arctic calanoid copepods have a variety of antioxidant defense systems to regulate the levels of potentially harmful reactive oxygen species generated by ultraviolet radiation in the Arctic marine ecosystem. However, information on the antioxidant activity of TCTP in the Arctic Calanus glacialis is still scarce. To understand the putative antioxidant function of the Arctic copepod C. glacialis TCTP (Cg-TCTP), its gene was cloned and sequenced. The Cg-TCTP comprised 522 bp and encoded a 174-amino acid putative protein with a calculated molecular weight of ~23 kDa. The recombinant Cg-TCTP (Cg-rTCTP) gene was overexpressed in Escherichia coli (BL21), and Cg-rTCTP-transformed cells were grown in the presence or absence of H₂O₂. Cg-rTCTP-transformed E. coli showed increased tolerance to high H₂O₂ concentrations. Therefore, TCTP may be an important antioxidant protein related to tolerance of the Arctic copepod C. glacialis to oxidative stress in the harsh environment of the Arctic Ocean.
배아 이식에서 임신율에 영향을 주는 요소들: 다변인 분석
홍유경 ( Yu Kyung Hong ),서은지 ( Eun Jee Seo ),안창숙 ( Chang Sook An ),구지선 ( Jee Sun Gu ),차병헌 ( Byung Hun Cha ),박은주 ( Eun Joo Park ),박원일 ( Won Il Park ),이진용 ( Jin Yong Lee ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.11
목적: 배아 이식 단계는 보조 생식 시술의 성공에 중요한 부분을 차지해 왔지만, 그에 비해 그동안 배아 이식 기술에 대한 충분한 연구 평가가 이루어지지 않았었다. 본 연구의 목적은 이식 시술의 다양한 요인들이 시술 성공률에 미치는 영향에 대해 조사하는 것이다. 연구 방법: 이식관의 종류 (부드러운 혹은 단단한), 이식관에 피가 묻었는지의 여부, 이식의 난이도 (쉬움, 중등도, 힘듦), 그리고 시술 의사를 변수로 하여 체외수정의 결과, 즉 임상적 임신율 (초음파로 태아 심박동 확인)과 출산 임신율의 차이를 분석하였다. 그 외 환자의 나이, 총 획득 난자 수, 자궁내막 두께, 이식한 배아 중 최상급 배아의 질 등을 결과에 영향을 미칠 수 있는 매개변수로 선정하였다. 단일 변인 분석은 카이자승 검정을, 다변인 분석은 로지스틱 회귀 분석을 사용하여 결과를 얻었다. 결과: 배아 이식 시술의 여러 요인들 중에 임신율과 생존 출산율 모두에 유의한 영향을 미치는 요인은 시술 난이도로 나타났다. 쉬운 이식과 중등도 이식 사이의 시술 성공률은 차이가 있었으나, 중등도와 힘든 이식의 차이는 없었다. 또한 이식관에 피가 묻었는지의 여부가 생존 출산율에 유의한 영향을 미치는 것으로 나타났다. 그 외 이식관의 종류와 시술 의사는 임신율, 출산율에 의미 없는 것으로 나타났다. 결론: 보조 생식 시술의 결과에 영향을 미치는 중요한 요인으로 이식관에 피가 묻었는지의 여부와 배아 이식 시술의 난이도를 들 수 있다. Objective: Embryo transfer is a critical step in assisted reproduction. However, the different aspects of the procedure were not fully estimated. The aim of this study is to evaluate the impact of each aspect in the procedure to success of pregnancy. Methods: Medical records of 525 fresh IVF/ICSI cycles were reviewed retrospectively. All embryo transfers were performed under ultrasonography guidance and after removal of cervical mucus. Assessed primary variables are difficulty of procedure (easy, moderate, and difficult), presence of blood in the catheter, types of catheter (soft or hard) and physician factor, and confounding variables are age of patient, total number of oocytes, number of transferred embryos, the highest grade of embryo, endometrial thickness. Measured outcomes were clinical pregnancy (visible fetal heart beat by ultrasonography) and live-birth rate. Univariate analysis and multivariate analysis by logistic regression test were used. Results: Among the variables of embryo transfer procedure, the difficulty of procedure was the only significant factor influencing both clinical pregnancy (OR: 0.402, 95% CI: 0.205-0.789, easy vs. moderately difficult) and live birth rate (OR: 0.380, 95% CI: 0.178-0.841, easy vs. moderately difficult). The difference between moderately difficult and very difficult procedure was not significant. Presence of blood was significant for live-birth rate (OR: 0.512, 95% CI: 0.267-0.980). Types of catheter and physician factor were not significant factors. Conclusion: The difficulty of the transfer procedure and presence of blood in catheter are significant factors influencing ART outcome.