만삭전 조기양막파열 산모의 양수내 항산화능

김윤하(Yoon Ha Kim),안봉환(Bong Whan Ahan),양성열(Sung Yeul Yang),김현주(Hyun Joo Kim),이경철(Kyung Chul Lee),김석모(Seok Mo Kim),송태복(Tae Bok Song),변지수(Ji Soo Byun) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.4

N/A Objective : This study was to determine the levels of cytokines, lipid peroxides, and total peroxyl radical-trapping antioxidative prameter (TRAP) values of amniotic fluid in pregnant women with preterm premature rupture of membranes (PPROM). Method : Amniotic fluid samples were obtained by amniocenteses for chromosomal analyses and other indications(control, n=26). Amniotic fluid samples were also obtained by amnioceteses from pregnant women with preterm premature rupture of membranes (n=22). Amniotic fluid cytokines(IL-6, IL-8) were determined by enzyme-linked immunosorbent assay (ELISA). Lipid peroxide levels of amniotic fluid were measured by thiobarbituric acid (TBA) reaction. The TRAP value of amniotic fluid was assessed by measuring the rate of oxygen consuming during controlled lipid peroxidation by 2,2'-azobis-2-amidinopropane hydrochloride, a water soluble peroxyl radical generator. Ascorbic acid and uric acid were measured by high performance liquid chromatography (HPLC) CoulArray detector of water-soluble antioxidants. Retinol, α-tocopherol, and γ- tocopherol were measured by HPLC-CoulArray detector of fat-soluble vitamins. Results : Cytokine levels of amniotic fluid in pregnant women with PPROM(IL-6: 23.37±10.61 ng/ml, IL-8: 10.23±3.04 ng/ml) were significantly higher than those in normal pregnant women(IL-6: 0.62±0.32 ng/ml, IL-8: 0.55 ± 0.15 ng/ml), (p<0.05). Lipid peroxide levels of the amniotic fluid in pregnant women with PPROM(10.42±1.11 nmol/mg protein) were significantly higher than those in normal pregnant women (7.32±0.65 nmol/mg protein), (p<0.05). The amniotic fluid obtained from pregnant women with PPROM showed significantly lower TRAP values (0.46±0.04 mM) than those from normal pregnant women(0.62±0.03 mM), (p<0.01). Amniotic fluid ascorbic acid levels in pregnant women with PPROM (52.1±15.1 nmol/ml) were significantly lower than in normal pregnant women(231.9±33.9 nmol/ml), (p<0.01). There were no significant differences of amniotic fluid uric acid, retinol, α-tocopherol, and γ-tocopherol levels between these groups. Conclusions : This findings suggest that a low antioxidant activity in amniotic fluid may cause PPROM and demonstrate that antioxidant vitamin, ascorbic acid, may act an important factor in the prevention of PPROM.

자간전증에서 제대정맥혈장과 태반내 단백산화 촉진능 증가

김은미 ( Eun Mi Kim ),김윤하 ( Yoon Ha Kim ),안봉환 ( Bong Whan Ahn ),양성열 ( Sung Yeul Yang ),김철홍 ( Cheol Hong Kim ),조문경 ( Moon Kyoung Cho ),김석모 ( Seok Mo Kim ),송태복 ( Tae Bok Song ) 대한주산의학회 2004 Perinatology Vol.15 No.1

목적 : 단백질 산화를 잘 일으키는 cephalosporin에 의한 정상과 자간전증 임부의 제대정맥혈장과 태반내 단백산화 촉진능의 차이를 비교하고 단백산화촉진활성이 자간전증의 병태생리에 어떤 역할을 하는가 규명하고자 하였다. 연구 방법 : 임신 34주 이상의 정상 임부 12명과 자간전증 환자 12명으로부터 태아분만시 제대정맥을 채취하고 분만 후 태반조직을 얻었다. Thiobarbituric acid 반응을 이용하여 지질과산화물 농도를 측정하였고, 2,4-dinitrophenylhydrazine (DNPH) 방법을 이용하여 단백질의 carbonyl기 함량을 측정하였다. 1 mM methodmoxalactam과 cephalothin 0.2 mL에 5시간 동안 함께 부치시킨 후 생성되는 제대정맥 혈장과 태반조직 단백질의 carbonyl기 함량을 측정하였다. 결과 : 정상 임신군의 제대정맥혈장과 태반조직균질액내 지질과산화물 치는 4.9±2.1과 5.8±2.2 nmol/mg protein이었으며, 자간전증군의 제대정맥혈장과 태반조직균질액내 지질과산화물은 10.1±4.0와 8.2±3.3 nmol/mg protein으로 자간전증군에서 정상 임신군보다 모두 의의있게 높았다 (p<0.05). Moxalactam과 cephalothin과 함께 부치시켰을 때 생성되는 제대정맥 혈장의 carbonyl기 함량은 자간전증 임부에서 정상임부에 비해 의의있게 높았다(8.5±2.0 vs. 6.6±1.4 nmol/mg protein, p<0.05, 7.6±1.6 vs. 6.2±1.2 nmol/mg protein, p<0.05). Moxalactam과 cephalothin과 함께 부치시켰을 때 생성되는 태반조직 단백질의 carbonyl기 함량은 자간전증 임부에서 정상임부에 비해 의의있게 높았다(17.6±5.3 vs. 13.0±4.2 nmol/mg protein, p<0.05, 16.1±5.2 vs. 12.5±4.4nmol/mg protein, p<0.05). 제대정맥 혈장과 태반조직내 지질과산화물 농도가 증가할수록 moxalactam과 cephalothin에 부치 후 단백질의 carbonyl기 함량이 증가하는 비례적인 관계를 보였다(p<0.01). 결론 : 자간전증 임부의 제대정맥 혈장과 태반조직에서 cephalosporin에 의한 단백질의 산화적 변화가 증가되어 있으며, 이러한 단백산화촉진에 관여하는 물질로는 제대정맥 혈장과 태반조직에서 증가되어 있는 지질과산화물이 작용을 할 것으로 사료되었다. 단백산화 촉진활성의 증가는 지질과산화물질의 증가와 더불어 자간전증의 발생에 관여하고 있을 것으로 사료되었다. Objective : This study was performed to compare the prooxidative activity stimulating the protein carbonyl formation by cephalosporins in the umbilical venous and placenta of preeclampsia with that of normal pregnancy. Methods : Lipid peroxide levels in the umbilical venous plasma and placental tissue homogenates of normal pregnancy (n=12) and preeclampsia (n=12) were measured by thiobarbituric acid reaction. The basal protein carbonyl contents in the umbilical venous plasma and placental tissue homogenates of normal pregnancy (n=12) and preeclampsia (n=12) were determined by the 2,4-dinitrophenylhydrazine (DNPH) method. After samples of them were mixed and incubated up to 5 hours with 0.2 mL of 1 mM moxalactam or cephalothin, the protein carbonyl contents in them were measured by DNPH. Results : Protein carbonyls formation by moxalactam and cephalothin in the umbilical venous plasma and of women with preeclampsia were significantly higher than that of women with normal pregnancy (8.5±2.0 vs. 6.6±1.4 nmol/mg protein, p<0.05, 7.6±1.6 vs. 6.2±1.2 nmol/mg protein, p<0.05). Protein carbonyls formation by moxalactam and cephalothin in the placental tissue homogenates of women with preeclampsia were significantly higher than that of women with normal pregnancy (17.6±5.3 vs. 13.0±4.2 nmol/mg protein, p<0.05, 16.1±5.2 vs. 12.5±4.4 nmol/mg protein, p<0.05). There were significant positive correlations between lipid peroxide and cephalosporins induced protein carbonyls levels of umbilical venous plasma, and placental tissue homogenates (p<0.01). Conclusion : These results suggest that increase in the prooxidative activity stimulating the oxidative modification of proteins in placenta may be involved in the pathogenesis of preecalmpsia.

鐵이온에 衣한 Hemoglobin의 酸化

李民化,梁誠烈,朴昞柱,姜亨求 全南大醫大 1986 전남의대학술지 Vol.23 No.2

赤血球膜蛋白의 變化에 依한 赤血球變形能의 變動

李民化,李起榮,梁誠烈,朴昞柱,尹士炫 全南大醫大 1985 전남의대학술지 Vol.22 No.1

Insulin의 血球膜과의 結合

李民化,安鳳煥,梁誠烈,朴昞柱 全南大醫大 1983 전남의대학술지 Vol.20 No.4

末梢組織의 Insulin 感受性에 미치는 殺鼠劑 Vacor의 影響

李民化,李泰熙,梁誠烈,朴昞柱 全南大醫大 1984 전남의대학술지 Vol.21 No.1

Ascorbin酸의 自動酸化에 依한 Superoxide 이온의 發生

李民化,李起榮,張香模,梁誠烈,朴昞柱 全南大醫大 1985 전남의대학술지 Vol.22 No.2

급성 Virus 성 간염 환자에서 고속액체 Chromatography 를 이용한 혈청 담즙산의 분석

이민화,이태희,김세종,정민영,양성열,장곡천수,마장무명 대한내과학회 1986 대한내과학회지 Vol.31 No.5

The physiologic status of enterhepatic bile flow is changed in hepatobiliary tracts and gastroenteric disorders. Recently it is reported as a diagnostic tool in liver diseases to check a serum bile acid level. We have checked serum total bile acid level and its 15 fractions in 13 cases of control group and 5 cases of acute viral hepatitis group by high-performance liquid chromatography. The results were as follows: 1) In control group, serum total bile acid levels at the fasting, and postprandial 1 and 2 hours were 10.39±3.15, 13.91±2.58, 12.71±3.27 mmol/L (mean±SD), respectively. In the levels of 15 fractions of serum total pile acid at the fasting, and postprandial 1 and 2 hours, deoxycholic acid were 2.89±1.51, 3.74±1.42, 3,43±2.26 mmol/L, glycocholic acid 1.16±0.5, l.80±0.58, l.23±0.56 mmol/L in the order of concentration. 2) In acute viral hepatitis group, serum total bile acid levels at the fasting and postprandial 2 hours were 80.78±2.25, 93.76±20.56 mmol/L, In the levels of 15 fractions of serum total bile acid, glycochenodeoxycholic acid were 31.58±11.11, 37.94±7.00 mmol/L, glycocholic acid 16.50±3.18, 21.27±6.03 mmol/L, taurochenodeoxycholic acid 15.44±8.50, 15.38±5.94 mmol/L, ursodeoxycholic acid 7.61±2.47, 6.86±2.21 mmol/L in the order of concentration. Cholic acid was 4.9, glycocholic acid 17.2, chenoxycholic acid 3.1, glycochenodeoxycholic acid 31.6, taurochendeoxycholi acid 13.9, and tauroursodeoxycholic acid 8, 1 times higher in acute viral hepatitis group than those of control group.