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SIRT1 Knockdown Enhances the Differentiation of Human Embryonic Stem Cells into Pancreatic β Cells
서난희,송화령,한명관 한국발생생물학회 2019 발생과 생식 Vol.23 No.4
Nicotinamide is used to maturate pancreatic progenitors from embryonic stem cells (ESCs) into insulinproducing cells (IPCs). It has been known that nicotinamide inhibits the enzymatic activity of SIRT1, an NAD+ -dependent deacetylase. Here we show that SIRT1 knockdown enhances the differentiation of human ESCs into IPCs. SIRT1 knockdown enhances the clustering size of IPCs and the expression of pancreatic genes including c-peptide, pancreas/duodenum homeobox protein 1 (PDX1), insulin, somatostatin, glucagon and Nkx6.1 in human ESC-derived IPCs. In addition, We found that IPCs differentiated from SIRT1 knockdowned human ESCs have more zinc compared to those from control human ESCs. Our data suggest that SIRT1 negatively regulates the differentiation of β cells from human ESCs.
Suppression of the ERK–SRF axis facilitates somatic cell reprogramming
허세종,송화령,정극래,장혜진,서난희,이주현,이지연,이병선,최현우,도정태,김진수,이수홍,정재원,이태규,심재경,한명관,이태희 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-
The molecular mechanism underlying the initiation of somatic cell reprogramming into induced pluripotent stem cells (iPSCs) has not been well described. Thus, we generated single-cell-derived clones by using a combination of drug-inducible vectors encoding transcription factors (Oct4, Sox2, Klf4 and Myc) and a single-cell expansion strategy. This system achieved a high reprogramming efficiency after metabolic and epigenetic remodeling. Functional analyses of the cloned cells revealed that extracellular signal-regulated kinase (ERK) signaling was downregulated at an early stage of reprogramming and that its inhibition was a driving force for iPSC formation. Among the reprogramming factors, Myc predominantly induced ERK suppression. ERK inhibition upregulated the conversion of somatic cells into iPSCs through concomitant suppression of serum response factor (SRF). Conversely, SRF activation suppressed the reprogramming induced by ERK inhibition and negatively regulated embryonic pluripotency by inducing differentiation via upregulation of immediate early genes, such as c-Jun, c-Fos and EGR1. These data reveal that suppression of the ERK-SRF axis is an initial molecular event that facilitates iPSC formation and may be a useful surrogate marker for cellular reprogramming.
저소득층 에너지효율개선사업 시행시 유전 알고리즘을 적용한 최적화 계획에 관한 연구
최세영(Choi, Se-Yeong),장예지(Jang, Ye-Ji),송화령(Song, Hwa-Ryeong),최정민(Choi, Jeong-Min) 대한건축학회 2018 대한건축학회 학술발표대회 논문집 Vol.38 No.2
The purpose of this study is to present the basic data that can be used for the optimal planning with genetic algorithm considering the support cost and gas energy consumption for heating by major unit improvement items such as insulation, windows, and infiltration in the energy efficiency improvement projects of low income housing. As a result, these findings in this study will contribute to the reduction of national greenhouse gas emissions as well as the improvement of the thermal comfort of low-income housing.