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HSP60 is required for stemness and proper differentiation of mouse embryonic stem cells
서난희,이은혜,서진희,송은경,한명관 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-
Embryonic stem cells (ESCs) are metabolically distinct from their differentiated counterparts. ESC mitochondria are less complex and fewer in number than their differentiated progeny. However, few studies have examined the proteins responsible for differences in mitochondrial structure and function between ESCs and somatic cells. Therefore, in this study, we aimed to investigate the differences between mitochondrial proteins in these two cell types. We demonstrate that HSP60 is more abundant in mouse ESC mitochondria than in mouse embryonic fibroblasts. Depletion of HSP60 inhibited mouse ESC proliferation and self-renewal, characterized by decreased OCT4 expression. HSP60 depletion also enhanced apoptosis during mouse ESC differentiation into embryoid bodies. Our results suggest that HSP60 expression has an essential role in ESC selfrenewal and survival of differentiated cells from ESCs.
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SIRT1 Knockdown Enhances the Differentiation of Human Embryonic Stem Cells into Pancreatic β Cells
서난희,송화령,한명관 한국발생생물학회 2019 발생과 생식 Vol.23 No.4
Nicotinamide is used to maturate pancreatic progenitors from embryonic stem cells (ESCs) into insulinproducing cells (IPCs). It has been known that nicotinamide inhibits the enzymatic activity of SIRT1, an NAD+ -dependent deacetylase. Here we show that SIRT1 knockdown enhances the differentiation of human ESCs into IPCs. SIRT1 knockdown enhances the clustering size of IPCs and the expression of pancreatic genes including c-peptide, pancreas/duodenum homeobox protein 1 (PDX1), insulin, somatostatin, glucagon and Nkx6.1 in human ESC-derived IPCs. In addition, We found that IPCs differentiated from SIRT1 knockdowned human ESCs have more zinc compared to those from control human ESCs. Our data suggest that SIRT1 negatively regulates the differentiation of β cells from human ESCs.
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청소년 자녀를 둔 학부모의 의사소통능력 증진을 위한 동기면담 훈련 경험 탐색
서난희 ( Seo Nan Hee ),김희정 ( Kim Hee Jung ) 한국간호과학회 정신간호학회(구 대한간호학회정신간호학회) 2018 정신간호학회지 Vol.27 No.3
Purpose: The study’s aim was to describe the experience of motivational interviewing (MI) training for parents with adolescents. Methods: Focus group interviews were carried out with 12 mothers in 3 groups who participated in the MI training. The study had a qualitative descriptive design, and qualitative content analysis was used. Results: Data analysis was separated into 4 domains: acceptance, relational skills, self-reflection, and rebuilding relationships. Seven categories and 11 subcategories included (1) enhancing acceptance and empathy, (2) recognizing the importance of the MI spirit and applying core skills, (3) improving self-expression and self-control of negative emotions and behavior, (4) recognizing self-problems, and (5) true communication and recovery of trust. Conclusion: Through MI training, participants have experienced not only the enhancement of their communication skills but also personal modifications (in self-acceptance, self-reflection, and self-control) and positive interpersonal relationships (rebuilding relationships). In addition, participants have realized the importance of listening and reflection in such experiences. Reflection training has provided participants meaningful experiences. Our key insight from these findings is that skill is not most important in communication training. MI elements such as acceptance, collaboration, and a respectful attitude are more likely to be used than conventional communication skills.
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Suppression of the ERK–SRF axis facilitates somatic cell reprogramming
허세종,송화령,정극래,장혜진,서난희,이주현,이지연,이병선,최현우,도정태,김진수,이수홍,정재원,이태규,심재경,한명관,이태희 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-
The molecular mechanism underlying the initiation of somatic cell reprogramming into induced pluripotent stem cells (iPSCs) has not been well described. Thus, we generated single-cell-derived clones by using a combination of drug-inducible vectors encoding transcription factors (Oct4, Sox2, Klf4 and Myc) and a single-cell expansion strategy. This system achieved a high reprogramming efficiency after metabolic and epigenetic remodeling. Functional analyses of the cloned cells revealed that extracellular signal-regulated kinase (ERK) signaling was downregulated at an early stage of reprogramming and that its inhibition was a driving force for iPSC formation. Among the reprogramming factors, Myc predominantly induced ERK suppression. ERK inhibition upregulated the conversion of somatic cells into iPSCs through concomitant suppression of serum response factor (SRF). Conversely, SRF activation suppressed the reprogramming induced by ERK inhibition and negatively regulated embryonic pluripotency by inducing differentiation via upregulation of immediate early genes, such as c-Jun, c-Fos and EGR1. These data reveal that suppression of the ERK-SRF axis is an initial molecular event that facilitates iPSC formation and may be a useful surrogate marker for cellular reprogramming.
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