꿀을 이용한 구강간호 프로그램이 항암화학요법을 받는 암환자의 구내염에 미치는 효과

송보람,박혜자 한국보건정보통계학회 2024 보건정보통계학회지 Vol.49 No.1

. Objectives: This study examined the effect of an oral care program with honey on chemotherapy-related mucositis among cancer patients. Methods: In this randomized quasi-experimental study, 34 participants were assigned to either a honey group (n = 17, swish-hold-swallow with acacia honey) or a saline control group (n = 17, mouthwashes with normal saline). The 14-day oral care program comprised a 20-30 minute’s single education session and 1 minute‘s swishing with acacia honey or 3 minutes’ mouthwashes with normal saline solution for 4 times/day (after three meals and before bed time). Mucositis score was assessed at the time of enrollment, day 7, and day 14 using the Oral Assessment Guide. Data were analyzed with repeated measures of ANCOVA and repeated measures of ANOVA with Bonferroni’s correction. Results: There was no significant interaction between groups and follow-up times in the mucositis scores. Within the honey group, there was a significant increment in the mucositis score at day 7 (t = 6.42, p < 0.001) and a signifi- cant decrement at day 14 (t = 11.02, p < 0.001). In the saline group, there was a significant increment in the mucositis score at day 7 (t = 9.62, p < 0.001) and a significant decrement at day 14 (t = -10.03, p < 0.001). Conclusions: A supportive oral care program using honey or saline may be suggested to ameliorate the chemotherapy related mucositis as a safe, feasible, and appropriate care for cancer patients.

Clear Cell Adenocarcinoma of Urethra: Clinical and Pathologic Implications and Characterization of Molecular Aberrations

송보람,이석현,박정환,문경철 대한암학회 2024 Cancer Research and Treatment Vol.56 No.1

Purpose This study aimed to evaluate the molecular features of clear cell adenocarcinoma (CCA) of the urinary tract and investigate its pathogenic pathways and possible actionable targets.Materials and Methods We retrospectively collected the data of patients with CCA between January 1999 and December 2016; the data were independently reviewed by two pathologists. We selected five cases of urinary CCA, based on the clinicopathological features. We analyzed these five cases by whole exome sequencing (WES) and subsequent bioinformatics analyses to determine the mutational spectrum and possible pathogenic pathways.Results All patients were female with a median age of 62 years. All tumors were located in the urethra and showed aggressive behavior with disease progression. WES revealed several genetic alterations, including driver gene mutations (<i>AMER1, ARID1A, CHD4, KMT2D, KRAS, PBRM1</i>, and <i>PIK3R1</i>) and mutations in other important genes with tumor-suppressive and oncogenic roles (<i>CSMD3, KEAP1, SMARCA4</i>, and <i>CACNA1D</i>). We suggest putative pathogenic pathways (chromatin remodeling pathway, mitogen-activated protein kinase signaling pathway, phosphoinositide 3-kinase/AKT/mammalian target of rapamycin pathway, and Wnt/β-catenin pathway) as candidates for targeted therapies.Conclusion Our findings shed light on the molecular background of this extremely rare tumor with poor prognosis and can help improve treatment options. Purpose This study aimed to evaluate the molecular features of clear cell adenocarcinoma (CCA) of the urinary tract and investigate its pathogenic pathways and possible actionable targets. Materials and Methods We retrospectively collected the data of patients with CCA between January 1999 and December 2016; the data were independently reviewed by two pathologists. We selected five cases of urinary CCA, based on the clinicopathological features. We analyzed these five cases by whole exome sequencing (WES) and subsequent bioinformatics analyses to determine the mutational spectrum and possible pathogenic pathways. Results All patients were female with a median age of 62 years. All tumors were located in the urethra and showed aggressive behavior with disease progression. WES revealed several genetic alterations, including driver gene mutations (AMER1, ARID1A, CHD4, KMT2D, KRAS, PBRM1, and PIK3R1) and mutations in other important genes with tumor-suppressive and oncogenic roles (CSMD3, KEAP1, SMARCA4, and CACNA1D). We suggest putative pathogenic pathways (chromatin remodeling pathway, mitogen-activated protein kinase signaling pathway, phosphoinositide 3-kinase/AKT/mammalian target of rapamycin pathway, and Wnt/β-catenin pathway) as candidates for targeted therapies. Conclusion Our findings shed light on the molecular background of this extremely rare tumor with poor prognosis and can help improve treatment options.

토코페롤의 대장암세포 성장, 침윤, 부착에 대한 억제 효과

송보람,주지형 충북대학교 생활과학연구소 2013 생활과학연구논총 Vol.17 No.2

The aim of the study was to investigate the inhibitory effects of different forms of tocopherols (T) on cell growth, invasion, and attachment in HCT116 human colon cancer cells. Treatment with γ-T and δ-T significantly inhibited cell growth (by 58.1-97.8% at the concentrations of 50-100 μM), invasion (by 49.4-87.0% at the concentrations of 20-80 μM), and attachment (by 28.2-75.4% at the concentrations of 50-100 μM). α-T, however, did not exhibit such inhibitory activities. δ-T was more active than γ-T in inhibiting cell attachment at the effective concentrations (p<0.05). These studies demonstrated the inhibitory activities of γ-T and δ-T against cell growth, invasion and attachment in HCT116 cells. More studies are needed in order to elucidate detailed mechanisms for the inhibitory action of γ-T and δ-T.

추출용매에 따른 들깨 추출물의 항산화 성분함량과 활성

송보람,주지형 충북대학교 생활과학연구소 2014 생활과학연구논총 Vol.18 No.2

The aim of the current study was to investigate the antioxidant contents and activities of perilla seed extracted by two different solvents, water and methanol. The total polyphenol and flavonoid levels of water extract of perilla seed (WP) were 383.5 mg gallic acid equivalent/100 g and 372.4 mg quercetin equivalent/100 g, respectively, and those were significantly higher than the corresponding levels of methanol extract of perilla seed (MP; 107.9 mg gallic acid equivalent/100 g and 233.9 mg quercetin equivalent/100 g, respectively; p<0.001). The radical scavenging activity of WP at the concentration of 350 μg/mL was 55.4%, and its ferric reducing antioxidant power reached to 98.1%. The radical scavenging activity and ferric reducing antioxidant power of MP (350 μg/mL) were similar to those of WP; 48.0% and 94.1%, respectively. These results indicate that water would be the better solvent for extracting antioxidant components from perilla seed than methanol.

Intraosseous Hibernoma: A Rare and Unique Intraosseous Lesion

송보람,문경철,류혜진,이철 대한병리학회 2017 Journal of Pathology and Translational Medicine Vol.51 No.5

Background: Hibernoma is a rare benign tumor of adults that is composed of multivacuolated adipocytes resembling brown fat cells. Hibernoma typically occurs in soft tissue, and intraosseous examples are very rare. Intraosseous hibernomas can radiologically mimic metastatic carcinoma and other tumorous conditions. Methods: To collect the intraosseous hibernomas, we searched the pathologic database and reviewed the hematoxylin and eosin (H&E)–stained slides of bone biopsy samples performed to differentiate radiologically abnormal bone lesions from 2006 to 2016. A total of six intraosseous hibernoma cases were collected, and clinical and radiological information was verified from electronic medical records. H&E slide review and immunohistochemical staining for CD68, pan-cytokeratin, and S-100 protein were performed. Results: Magnetic resonance imaging of intraosseous hibernomas showed low signal intensity with slightly hyperintense foci on T1 and intermediate to high signal intensity on T2 weighted images. Intraosseous hibernomas appeared as heterogeneous sclerotic lesions with trabecular thickening on computed tomography scans and revealed mild hypermetabolism on positron emission tomography scans. Histopathologically, the bone marrow space was replaced by sheets of multivacuolated, foamy adipocytes resembling brown fat cells, without destruction of bone trabeculae. In immunohistochemical analysis, the tumor cells were negative for CD68 and pan-cytokeratin and positive for S-100 protein. Conclusions: Intraosseous hibernoma is very rare. This tumor can be overlooked due to its rarity and resemblance to bone marrow fat. Pathologists need to be aware of this entity to avoid misdiagnosis of this rare lesion.

기획특집 : 기후변화와 식품안전 간 융합 데이터베이스 및 데이터 마이닝 시스템 구축

송보람 ( Bo Ram Song ),이헌주 ( Hun Joo Lee ) 한국식품위생안전성학회 2016 Safe Food Vol.11 No.1

토코페롤의 대장암세포 성장, 침윤, 부착에 대한 억제 효과

송보람, 주지형 충북대학교 생활과학연구소 2013 생활과학연구논총 Vol.17 No.2

The aim of the study was to investigate the inhibitory effects of different forms of tocopherols (T) on cell growth, invasion, and attachment in HCT116 human colon cancer cells. Treatment with γ-T and δ-T significantly inhibited cell growth (by 58.1-97.8% at the concentrations of 50-100 μM), invasion (by 49.4-87.0% at the concentrations of 20-80 μM), and attachment (by 28.2-75.4% at the concentrations of 50-100 μM). α-T, however, did not exhibit such inhibitory activities. δ-T was more active than γ-T in inhibiting cell attachment at the effective concentrations (p<0.05). These studies demonstrated the inhibitory activities of γ-T and δ-T against cell growth, invasion and attachment in HCT116 cells. More studies are needed in order to elucidate detailed mechanisms for the inhibitory action of γ-T and δ-T.

UV조사에 의해 유발된 SKH-1 hairless 마우스의 피부노화에 미치는 한천마스크의 개선효과

송보람(Bo Ram Song),김지은(Ji Eun Kim),윤우빈(Woo Bin Yun),이미림(Mi Rim Lee),최준영(Jun Young Choi),박진주(Jin Ju Park),김동섭(Dong Seob Kim),이충열(Chung Yeoul Lee),이희섭(Hee Seob Lee),임용(Yong Lim),정민욱(Min Wook Jung),김배환(Bae 한국생명과학회 2017 생명과학회지 Vol.27 No.9

UV 조사에 의해 유도된 광노화(photoaging)에 대한 한천젤마스크(agar gel mask, AGM)의 효과를 평가하기 위해, SKH-1 hairless 마우스의 피부에 4주간의 UV조사와 AGM 및 에센스처리 한천젤마스크(agar gel mask dipped in essence, AGMdE)를 동시에 처리한 후 피부주름, 피부표현형, 피부조직구조, 항산화조건 및 독성 등을 분석하였다. AGM과 AGMdE의 부착은 주름형성의 억제, 홍반지수의 감소, 피부수분의 증진뿐만 아니라 경피 수분 손실(TEWL)을 방지하였다. 또한, 표피두께는 UV+Vehicle 처리군과 비교했을 때, UV+AGM 및 UV+AGMdE 처리군에서 UV를 조사하지 않은 그룹과 유사한 수준으로 회복되었다. MMP-1과 tyrosinase의 발현수준은 UV+Vehicle 처리군에서 가장 높게 증가하였으나 UV+AGM 및 UV+AGMdE 처리군에서 유의적으로 감소하였다. 더불어, SOD 활성은 UV+Vehicle 처리군에 비하여 UV+AGM 및 UV+AGMdE 처리군에서 유의적으로 감소되었지만, 대부분의 독성화합물 처리에 의해 유도되는 혈청생화학적 지표와 조직학적 특성의 변화는 UV+AGM 및 UV+AGMdE 처리군에서 관찰되지 않았다. 따라서, 이러한 결과들은 AGM이 피부형태, 조직학적 구조, 산화적 상태를 조절함으로써 피부노화를 예방하는 마스크팩으로 활용될 수 있는 가능성을 있음을 제시한다. To investigate the beneficial effects of an agar gel mask (AGM) on UV-induced photoaging, SKH-1 hairless mice were treated with a topical application of AGM and an AGM dipped in essence (AGMdE). The mice were divided into an no radiation group, UV + AGM, UV + AGMdE, and UV + vehicle (PBS) treatment groups. Alterations in skin wrinkles, skin phenotype, histological structures, oxidative status, and toxicity were then evaluated during 4 weeks of exposure. The topical application of AGM and AGMdE inhibited wrinkle formation, suppressed the erythema index, prevented transepidermal water loss, and enhanced skin hydration. In addition, epidermal thickness recovered to a similar level as that in the no irradiation group in the UV + AGM and UV + AGMdE treatment groups compared with the UV + vehicle (distilled water) group. Furthermore, the expression levels of matrix metalloproteinase-1 (MMP-1) and tyrosinase were reduced in the UV + AGM and UV + AGMdE treatment groups, although the highest level varied. Moreover, superoxide dismutase (SOD) activity was significantly lower in the UV + AGM and UV + AGMdE treatment groups as compared with the UV + vehicle group. No significant alterations induced by most toxic compounds were measured in serum biochemical markers and liver and kidney histological features of the UV + AGM and UV + AGMdE treatment groups. These results suggest that AGM may protect against skin aging by regulating skin morphology, histopathological structures, and oxidative conditions.

들깨의 볶음처리가 헥산 추출유의 항산화 성분함량 및 활성에 미치는 영향

송보람(Bo ram Song),주지형(Ji hyeung Ju) 충북대학교 생활과학연구소 2015 생활과학연구논총 Vol.19 No.2

The aim of the current study was to investigate the effect of roasting on antioxidant contents and activities of perilla seed oil extracted by n-hexane. The total polyphenol level of roasted perilla seed extract (RP; 27.1 mg gallic acid equivalent/100 g) was similar to that of unroasted perilla seed extract (UP; 29.4 mg gallic acid equivalent/100 g). The total flavonoid level of RP (42.2 mg quercetin equivalent/100 g) was higher than that of unroasted perilla seed extract (UP; 31.1 mg quercetin equivalent/100 g; p<0.05). The radical scavenging activity and ferric reducing antioxidant power of RP at the concentration of 350 μg/mL was 30.9%, and 13.1%, respectively, which were higher than the corresponding levels of UP (27.6% and 7.4%, respectively; p<0.05). These results indicate that roasting is effective in increasing the total flavonoid content and antioxidant activities of perilla seed oil extracted by n-hexane.

β-Sitosterol이 HCT116 대장암세포의 콜로니형성 및 부착성에 미치는 효과

송보람(Bo ram Song),주지형(Ji hyeung Ju) 충북대학교 생활과학연구소 2016 생활과학연구논총 Vol.20 No.1

β-Sitosterol is the most abundant phytosterol present ubiquitously in plants. The aim of the current study was to investigate the inhibitory effects of β-sitosterol on colony formation and adhesion in HCT116 human colon cancer cells. β-Sitosterol significantly inhibited cell growth (to 50-89% of the control by the concentrations of 25-100 μM; p<0.01) and effectively changed nuclear morphology (by the concentration of 50 μM) in HCT116 cells. These results confirm previous findings on the growth-inhibitory and apoptosis-inducing activities of β-sitosterol in different cancer cell lines. Treatment with β-sitosterol resulted in significant inhibition of both colony formation (to 29% of the control by the concentration of 50 μM) and adhesion (to 44-54% of the control by the concentration of 25-100 μM) in HCT116 cells. These results suggest that β -sitosterol might modulate the self-renewal capacity of colon cancer stem cells as well as invasion and metastasis during colon carcinogenesis. More in-depth studies are needed in order to verify such actions and to elucidate underlying mechanisms.