Hyaluronan synthase 2, a target of miR-200c, promotes carbon tetrachloride-induced acute and chronic liver inflammation via regulation of CCL3 and CCL4

김선명,송가연,Shim Aeri,Lee Jee Hyung,엄철빈,Liu Cheng,양윤미,Seki Ekihiro 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Liver fibrosis occurs during wound healing after repeated liver injury and is characterized by extensive extracellular matrix deposition. We previously identified hyaluronan synthase 2 (HAS2) as a driver of liver fibrosis and hepatic stellate cell (HSC) activation. Developing strategies to suppress HSC activation is key to alleviating liver fibrosis, and HAS2 is an attractive candidate for intervention. To gain insight into the molecular function of HAS2, we investigated its posttranscriptional regulation. We found that miR-200c directly targets the 3’ untranslated regions of HAS2. Moreover, miR-200c and HAS2 were inversely expressed in fibrotic human and mouse livers. After establishing the direct interaction between miR-200c and HAS2, we investigated the functional outcome of regulating HAS2 expression in three murine models: CCl4-induced acute liver injury, CCl4-induced chronic liver fibrosis, and bile duct ligation-induced liver fibrosis. Hepatic Has2 expression was induced by acute and chronic CCl4 treatment. In contrast, miR-200c expression was decreased after CCl4 treatment. HSC-specific Has2 deletion reduced the expression of inflammatory markers and infiltration of macrophages in the models. Importantly, hyaluronidase-2 (HYAL2) but not HYAL1 was overexpressed in fibrotic human and murine livers. HYAL2 is an enzyme that can cleave the extracellular matrix component hyaluronan. We found that low-molecular-weight hyaluronan stimulated the expression of inflammatory genes. Treatment with the HA synthesis inhibitor 4-methylumbelliferone alleviated bile duct ligation-induced expression of these inflammatory markers. Collectively, our results suggest that HAS2 is negatively regulated by miR-200c and contributes to the development of acute liver injury and chronic liver inflammation via hyaluronan-mediated immune signaling.

Herbal Medicine for Liver Cancer: A Protocol for Systematic Review and Meta-Analysis

권승원,송가연,양승보 대한한방내과학회 2022 大韓韓方內科學會誌 Vol.43 No.4

Introduction: The aim of this systematic review is to provide evidence confirming the efficacy and safety of herbal medicines used in the treatment of liver cancer. Methods: The review will include randomized clinical trials that compared herbal medicines used as treatments for liver cancer with other therapies, such as placebos and Western medicine. Only randomized controlled trials will be included in this review, and all types of herbal medicine will be evaluated. Eleven electronic databases will be searched from the inception date: the Cochrane Database of Systematic Reviews, MEDLINE, EMBASE, AMED, CINAHL, one Chinese database (CNKI), and five Korean databases (OASIS, DBpia, RISS, KISS, and NDSL). The selection of studies, data extraction, and management will be performed independently by four researchers. Methodological quality, including the risk of bias, will be assessed using the Cochrane risk-of-bias assessment tool. Results: The review of current evidence for the effectiveness of herbal medicine for liver cancer will be summarized and quantitatively analyzed. Conclusions: Our systematic review will provide evidence of the efficacy of herbal medicines as treatments for liver cancer. This evidence will provide useful information for practitioners and patients in the fields of oncology and complementary medicine. Systematic review registration: 2021 CRD42021268386

대학생들을 위한 학습된 무기력 완화 온라인 자기관리 프로그램 개발과 참여 경험에 대한 연구: 개념도 연구방법의 활용

김민선,위고운,김세희,송가람,박희진,백정연,한승희 한국상담학회 2021 상담학연구 사례 및 실제 Vol.6 No.1

This study examined the perceptions of college students' experience of participating in online learned helplessness relief programs. In this study, a concept mapping method was used. Interview data of 17 college students who voluntarily participated in the online helplessness alleviation program were analyzed, and finally 62 statements were drawn. As a result of examining the distribution of statements through multidimensional analysis, the dimension of each statement was found to be the most appropriate in two dimensions, and the statements are distributed based on the x-axis (concentration on the present-past) and y-axis (individual activity-the desire for group activity). In addition, as a result of the second-stage cluster analysis through hierarchical cluster analysis and K-means cluster analysis, the perception of the lethargic mitigation program was determined in 6 clusters (cluster 1 = satisfaction with the convenience and activity of online self-management programs, cluster 2 = expectations for learning and change through lay down, cluster 3 = limitations and regrets of online self-management programs, cluster 4 = understanding of self, and the cause of helpless feelings, cluster 5 = difficulty in maintaining desire for interaction and motivation to participate, cluster 6 = acquisition of a new perspective on the problem and actual practice). Based on the above results, we discussed the implications that the helplessness relief program can give to the non-face-to-face online counseling program in the covid-19 pandemic situation. 본 연구에서는 대학생들을 대상으로 ‘학습된 무기력 완화 온라인 자기관리 프로그램’ 참여 경험을 살펴보았으며, 이를 위해 개념도 연구방법(concept mapping method)을 사용하였다. 연구를 위해, 온라인 프로그램에 자발적으로 참여한 17명의 대학생들의 인터뷰 자료를 분석하였으며, 최종적으로 62개의 진술문(statements)을 도출하였다. 다차원 분석 결과 진술문들의 분포를 2차원으로 설명하는 것이 가장 적절한 것으로 나타났으며, x축(현재-과거에의 집중), y축(개별적인 활동-집단 활동에 대한 욕구)을 기준으로 진술문들이 분포됨을 알 수 있었다. 또한 위계적 군집분석과 K-means 군집분석을 통한 2단계 군집분석결과, 무기력 완화 프로그램에 대한 지각을 총 6개의 군집(군집 1 = 온라인 자기관리 프로그램의 편의성과 활동에 대한 만족, 군집 2 = 내려놓음을 통한 배움과 변화에 대한 기대, 군집 3 = 온라인 자기관리 프로그램의 한계와 아쉬움, 군집 4 = 무기력한 감정의 원인과 자신에 대한 이해, 군집 5 = 상호작용에 대한 욕구와 참여 동기 유지의 어려움, 군집 6 = 문제를 보는 새로운 관점의 획득과 실천)으로 분류할 수 있었다. 위 결과를 바탕으로 코로나-19와 같은 감염병이 확산되는 상황에서 무기력 완화 자기관리 프로그램이 비대면 온라인 상담 프로그램에 줄 수 있는 함의를 논하였다.

디지털 인문학과 지식의 공동생산 ― 위키 플랫폼과 <한국 근대 지식인 아카이브> 편찬

장문석 ( Jang Moon-seok ),김윤진 ( Kim Yoonjin ),이은지 ( Lee Eunji ),송가배 ( Song Gabae ),고자연 ( Ko Ja-yeon ),김지선 ( Kim Ji-sun ) 서울대학교 인문학연구원 2021 人文論叢 Vol.78 No.1

이 글은 위키 플랫폼에 기초하여 진행한 <한국 근대 지식인 아카이브>를 중심으로 디지털 인문학과 지식의 공동생산이라는 논제와 그 가능성을 검토하였다. 디지털 환경에서 인문학 지식의 공동생산이라는 새로운 가능성이 열릴 수 있다는 것이 이 글의 논점이다. 2장에서는 위키 플랫폼의 글쓰기가 인문학 글쓰기에 제시하는 새로운 가능성을 검토하였다. 위키는 공유 작업에 적합한 플랫폼으로 공동연구의 과정을 확인할 수 있다. 동시에 위키는 디지털 환경을 적극적으로 활용한 조합적 글쓰기를 가능하도록 한다. 3장에서는 데이터에 기반한 지식의 공동편찬이 열어주는 새로운 인문학 지식과 질문의 발견 가능성을 검토하였다. 참여자들은 인문학 지식에 근거하여 공동으로 온톨로지를 설계했다. 그리고 온톨로지에 따라 입력한 데이터를 공유하고 활용할 때, 새로운 인문학적 논제를 발견하였다. 이 글은 디지털 환경에서 데이터를 활용한 공동 작업이 인문학 연구에 새로운 가능성을 열 수 있다는 사실을 확인하였다. 이후 본격적으로 데이터를 입력하면서, 이 글이 발견한 가능성을 현실화하는 것이 추후 과제이다. This article reviews the issue of digital humanities and co-production of knowledge around the Project of < Archive of Intellectuals in Modern Korea > based on the wiki platform and its possibilities. The argument of this article is that a new possibility of co-production of humanities knowledge can be opened in the digital environment. In Chapter 2, we consider what possibilities the writing of the Wiki platform could open to writing in humanities. Wiki is a suitable platform for sharing tasks and can identify the process of joint research. At the same time, Wiki enables combinatorial writing that actively utilizes the digital environment through hypertext and the function of extention and embedding of wiki. In Chapter 3, we discuss that data and co-production discover new humanistic issues. Participants jointly designed ontology based on humanities knowledge. In addition, when sharing and utilizing the data entered according to the ontology, a new humanistic thesis was discovered. This article confirms that collaboration using data in a digital environment can open new possibilities for humanities research. Afterwards, the task is to enter data in earnest and realize the possibility of this article’s discovery.