양성혈액배양에서 메치실린 내성 황색 포도알균의 직접검출을 위한 PBP2a 라텍스 응집법, PBP2a 신속 면역크로마토그래픽법 및 Chromogenic 배지법의 비교

홍승복,손보라,신경섭 대한임상미생물학회 2012 Annals of clinical microbiology Vol.15 No.1

Background: This study compared three non-molecular methods for the detection of methicillin-resistance directly from blood cultures containing Staphylococcus aureus: penicillin-binding protein (PBP) 2a latex agglutination (LA), PBP2a immunochromatographic assay (ICA) and MRSA chromogenic medium (CM). Methods: Fifty methicillin-resistant S. aureus (MRSA) and 50 methicillin-susceptible S. aureus (MSSA) were seeded into blood-culture bottles. When isolates returned a positive signal, 5 mL of culture was added to serum separator tubes and centrifuged at 1,300 g for 10 min. The pellets were then used as the inoculum for the PBP2a LA, MRSA-CM and PBP2a ICA. The pure colony was used for PBP2a LA test, additionally. Results: The respective sensitivities and specificities were 98 and 100% for PBP2a ICA, and 100 and 100% for MRSA-CM in direct detection of MRSA from positive blood culture. The results of PBP2a LA test using pure colony were entirely compatible with those by mecA gene PCR but the PBP2a LA test using the pellets directly isolated from positive blood culture showed sometimes ambiguous agglutination; its sensitivity and specificity were 78 and 100%, if ambiguous results were scored as negative, and were 90 and 92%, if ambiguous results were scored as positive, respectively. Conclusion: For direct detection of MRSA in positive blood culture, MRSA-CM and PBP2a ICA provided excellent results. The PBP2a LA test using pure colony also gave excellent results but the PBP2a LA test by the direct method using pellet of positive blood culture was slightly less sensitive than the other two methods.

Myroides species (Flavobacterium odoratum)에 의한 비뇨기계감염의 분자역학적 조사

김현수,홍승복,이도훈,김원식,손보라,신경섭 대한감염학회 2003 감염과 화학요법 Vol.35 No.4

목적 : Myroides species는 자연환경에 널리 분포하나 임상적으로 의미 있는 감염은 매우 드물다. 저자들은 이들 균주에 의한 지속적인 원내 요로 감염을 경험하였기에 보고하는 바이다. 방법 : 9개월간 25명의 환자, 44 소변 검체에서 Myroides species가 분리되었으며 이들 균주의 분자유전학적 아형 분류에 random amplified polymorphic DNA (RAPD) 방법을 적용하였다. 결과 : 25명 환자 모두는 카테터를 유치하고 있는 입원 환자였으며 5명의 환자는 농뇨를 동반하여 요로감염이 의심되었으나 다른 환자들은 카테터 유치에 의한 균집락화일 가능성이 있었다. 분리된 Myroides species는 검사한 모든 항균제에 내성을 보였고 RAPD 방법에 의한 분자유전학적 아형 분류에서 모두 동일한 유전자형을 나타내었다. 결론 : 원내 소변검체에서 지속적으로 동정된 Myroides species는 동일한 유전자형으로 밝혀졌으며 대부분의 항균제에 내성을 보이므로 이들 균주의 전파를 막는 것은 임상적으로 중요한 것으로 사료된다. Background : Myroides species are widely distributed in nature, but clinical infection by these organisms are extremely rare. We report herein prolonged outbreak of urinary tract infection by Myroides species. Methods : Forty-four Myroides spp. were isolated from urine samples from 25 patients over a period of nine months, and random amplified polymorphic DNA (RAPD) method was performed to characterize the genotype of these isolates. Results : All of the subjects were hospitalized patients with indwelling urinary catheter. Five of the patients showed concomitant pyuria, which could be considered as evidence of urinary tract infection, and isolation of these organisms in the remainder of the patients could be considered as simple colonization. All the isolates were resistant to antimicrobial agents tested. RAPD analysis showed identical DNA fingerprinting patterns in all the isolates. Conclusion : This study revealed that all the Myroides spp. isolated from urinary specimens of prolonged outbreak were genotypically the same. Because of its resistance to multiple antimicrobial agents, prevention of dissemination of this strain is clinically important.

유기농자재의 탄소배출량 산정을 위한 전과정평가(LCA) -참숯, 목초액, 미생물제재를 중심으로-

윤성이,손보홍,Yoon, Sung-Yee,Son, Bo-Hong 한국유기농업학회 2012 韓國有機農業學會誌 Vol.20 No.3

Since 1997, Korean Ministry of Knowledge Economy and Ministry of Environment have established data on some 400 basic raw and subsidiary materials and process like energy, petro-chemical, steel, cement, glass, paper, construction materials, transportation, recycling and disposal etc by initiating establishment of LCI database. Regarding agriculture, Rural Development Administration has conducted establishment of LCI database for major farm products like rice, barley, beans, cabbage and radish etc from 2009, and released that they would establish LCI database for 50 items until 2020 later on. The domestic LCI database for seeds, seedling, agrochemical, inorganic fertilizer and organic fertilizer etc is only at initial stage of establishment, so overseas LCI databases are brought and being used. However, since the domestic and overseas natural environments differ, they fall behind in reliability. Therefore, this study has the purpose to select organic farming materials, survey the production process for various types of organic farming materials and establish LCI database for the effects of greenhouse gas emitted during the process in order to select carbon basic units for agricultural production system compliant in domestic situation instead of relying on overseas data and apply life cycle assessment of greenhouse gas emitted by each crop during the process. As for selecting methods, in this study organic farming materials were selected in the method of direct observation of material and bottom-up method a survey method with focus on the organic farming materials admitted into rice production. For the basic unit of carbon emission amount by the production of 1kg of organic farming material, the software PASS 4.1.1 developed by Korea Accreditation Board under Ministry of Knowledge Economy was used. The study had the goal to ultimately provide basic unit to calculate carbon emission amount in executing many institutions like goal management system and carbon performance display system etc in agricultural sector to be conducted later on. As a result, emission basic units per 1kg of production were calculated to be 0.0088kg-$CO_2$ for charcoal, 0.1319kg-$CO_2$ for grass liquid, and 0.2804kg-$CO_2$ for microbial agent.

신생아 중환자실에서 발생한 Candida albicans 패혈증의 역학조사에 RAPD의 적용

신경섭,홍승복,신형식,박범수,손보라 대한임상병리학회 1999 대한임상병리학회지 Vol.19 No.4

Myroides species (Flavobacterium odoratum)에 의한 비뇨기계감염의 분자역학적 조사

김현수,홍승복,이도훈,김원식,손보라,신경섭 대한감염학회 2003 Infection and Chemotherapy Vol.35 No.4

Arcobacter butzleri에 의한 균혈증 1예

신경섭,손보라,홍승복 대한진단검사의학회 2005 Annals of Laboratory Medicine Vol.25 No.4

Arcobacter butzleri, a gram-negative, slightly curved bacterium previously described as an aerotolerant Campylobacter, was isolated from the blood of a 53-year-old male patient with alcoholic cirrhosis. The isolate was identified by various phenotypic tests and 16S rRNA sequencing analysis. The patient was treated with amikacin and recovered uneventfully. To our knowledge, this is the first case of A. butzleri bacteremia reported in Korea. Arcobacter butzleri는 만곡형 그람음성 간균으로 공기 중에서증식할 수 있으므로 이전에 aerotolerant Campylobacter로 불 리었다. 알코올간경화증을 앓는 53세 남자의 혈액에서 국내에서 최초로 A. butzleri를 분리하였으며, 다양한 생화학적 검사 및 16SrRNA 염기서열 분석을 통하여 동정하였다. 이 환자는 amikacin의 투여로 회복되었다.

Arthrobacter woluwensis에 의한 카테터 연관성 균혈증 1예

신경섭,홍승복,손보라 대한진단검사의학회 2006 Annals of Laboratory Medicine Vol.26 No.2

기회감염균으로 간주되고 있으며 catalase 양성, coryneform그람양성균인 Arthrobacter woluwensis가 대장암이 전이된 56세남자의 혈액에서 반복하여 분리되었다. 다양한 표현형 검사 및 16SrRNA 염기서열 분석을 통하여 균을 동정하였다. E-test를 이용한 감수성 검사에서 vancomycin의 MIC는 1.5 g/mL이었고,cefotaxime 및 penicillin의 MIC는 각 >64 g/mL과 4 g/mL이었다. 환자에게 vancomycin이 투여되었으며 감염원으로추정되는 쇄골하 정맥도관이 제거된 후 균은 더 이상 분리되지않았다. Arthrobacter는 생화학적 검사만으로 동정하기 어렵기때문에 A. woluwensis에 의한 인체 내 감염은 국내에서 아직 보고되지 않았을 가능성이 높다.

Characterization of Acinetobacter baumannii Co-producing Carbapenemases OXA-23 and OXA-66, and armA 16S Ribosomal RNA Methylase at a University Hospital in South Korea

정혜원,신경섭,손보라,신동익,류동희,홍승복,한규동 대한임상미생물학회 2011 Annals of clinical microbiology Vol.14 No.2

Background: In the present study, the resistance mechanisms against carbapenems and aminoglycosides for 23 strains of multi-drug-resistant Acinetobacter baumannii isolated at a university hospital were investigated. Methods: The minimal inhibitory concentrations (MICs) were determined via broth microdilution or Etest. The genes encoding OXA-type carbapenemases and 16S rRNA methylase were identified using multiplex PCR, and the amplified products were sequenced. Conjugation experiments were conducted, and an epidemiologic study was performed using enterobacterial repetitive intergenic consensus (ERIC)-PCR. Results: In the isolates, the MICs of the tested aminoglycosides, including arbekacin, were >1024 μg/mL; the MICs of aztreonam, cefepime, ceftazidime, and ciprofloxacin ranged from 64 to 128 μg/mL; and the MICs of carbapenem ranged from 32 to 64 μg/mL, as determined through the broth microdilution test. According to the E-test, the MICs of ampicillin/sulbactam and colistin were 8 and 0.25 to 0.38 μg/mL, respectively. Sequence analysis confirmed that all of the isolates expressed carbapenemases OXA-23 and OXA-66, as well as armA 16S rRNA methylase. In addition, ISAba1 was identified upstream of the gene encoding OXA-23. OXA-23 and armA were not transferred to Escherichia coli J53 cells in the transconjugation experiments. ERIC-PCR molecular fingerprinting produced a single pattern in all cases. Conclusion: The co-production of OXA-23 and armA 16S rRNA methylase may be attributed to the multidrug resistance of the A. baumannii isolates in the present study. Stricter surveillance and more rapid detection are necessary to prevent the spread of this type of resistance in the future.