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Immobilization and characterization of carbonic anhydrase with monosilicate and spermine
백승필,손려강,기미란,여기백,민기하 한국공업화학회 2014 한국공업화학회 연구논문 초록집 Vol.2014 No.1
A chemical absorption technology using amine aqueous solution to capture CO<sub>2</sub> causes by-product due to harsh condition process. It is an effective eco-friendly way to replace the amine solution with biocatalyst. But the CO<sub>2</sub> sequestration process is worked at harsh condition We found Hahella chejuensis carbonic anhydrase (HCCA) derived from domestic marine bacterium having high pH stability. To adjust CO<sub>2</sub> sequestration system, long time thermal stability and recyclable are necessary. We performed HCCA immobilization system based on silicification of a monosilicate, tetramethyl orthosilicate (TMOS), and spermine derived from organic compound having 4 amine group. Free HCCA lost their activity in 1 hour at 60 degree, in contrast, immobilzed HCCA by silica particle endured over 2hour with 80% activity. In 13 times recycling test, immobilized HCCA activity was maintained 80%. Thus monosilicate immobilization of HCCA is ready to adjust CO<sub>2</sub> sequestration system.
Effect of Human or Mouse IL-7 on the Homeostasis of Porcine T Lymphocytes
홍지화,김상훈,김현경,장준호,손려강,박성필,박영호,강필용,정강진,김지수,최한별,김선욱,정용우 대한면역학회 2021 Immune Network Vol.21 No.3
Due to the inconsistent fluctuation of blood supply for transfusion, much attention has been paid to the development of artificial blood using other animals. Although mini-pigs are candidate animals, contamination of mini-pig T cells in artificial blood may cause a major safety concern. Therefore, it is important to analyze the cross-reactivity of IL-7, the major survival factor for T lymphocytes, between human, mouse, and mini-pig. Thus, we compared the protein sequences of IL-7 and found that porcine IL-7 was evolutionarily different from human IL-7. We also observed that when porcine T cells were cultured with either human or mouse IL-7, these cells did not increase the survival or proliferation compared to negative controls. These results suggest that porcine T cells do not recognize human or mouse IL-7 as their survival factor.
Thi Khoa My Nguyen,기미란,손려강,김경희,홍정화,백승필 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.101 No.-
Silica-forming peptides (SFPs) and the SFP-modified structural proteins can be used as templates for thesynthesis of biosilica nanoparticles (NPs). However, such biomolecule-mediated synthesis showed limitationsfor the generation of NPs with sizes sub-50 nm. In this study, the SFP sequences(KPSHHHHHTGAN and KPTHHHHHHDG for Kps and Kpt, respectively) were fused to the C-terminus ofthe human ferritin heavy chain (Fn), resulting in the SFP moieties in the inner space of Fn (termed FncKpsand Fn-cKpt). Using Fn-cSFP templates for silicification in two-phase system, the Fn-cKps@SiO2and Fn-cKpt@SiO2 NPs were generated in mean diameters of 26 and 28 nm, respectively. Also, weemployed biosilica NPs sub-50 nm for a doxorubicin (Dox) delivery system (application model). FncKpt@SiO2 NPs exhibited a high loading efficiency compared to Fn-cKpt only (1.7-fold) and prolongedrelease patterns with Dox. Most importantly, the uptake of Fn-cKpt@SiO2 into cancer cells was increasedso that the efficient delivery of Dox to the cell inside was observed. The uniform generation of biosilicaNPs sub-50 nm obtained here is a new achievement. Together with the ideal pH-dependent drug release,the size-controlled design of biosilica will be a useful strategy for efficient delivery of chemical drugs totarget cells.