양서류의 수분평형에 미치는 항이뇨 호르몬작용의 계절적 변동

성양호,차영선 고려대학교 의과대학 1974 고려대 의대 잡지 Vol.11 No.2

The rate of water uptake across the skin and urine formation by the kidneys were measured in the living frog, (Rana Temporaria), especially under the influence of vasopressin, when frogs were kept in distilled water at 22℃. Adult frogs of both sexes weighing between 20 and 30g were used. Experiments were performed in four seasons throughout the year. At the onset of the experiment polyethylene catheter introduced into cloaca through cloaca orifice, and fixed After recovery frogs were placed in 200ml glass beakers containing 100ml of distilled water, oxygen supplied through polyethylene tube into outside bathing water. The amount of water uptake was calculated from the changes in body weight per unit time and then expressed as % of initial body weight. Vasopressin, (Pitressin, Parke Davis and Co.) 0.06 u/g B.W. diluted in 0.65% NaCl solution. The volume injected varied from 0.1 to 0.3ml for frog. All injection were given into lymphatic sac. The results obtained are summarized as follows : 1. The water uptake across the skin increased significantly after the injection of vasopressin in spring and autumn season whereas decreased in summer season. 2. Antidiuretic action of vasopressin by the kidneys were obtained in all the seasons except spring season. 3. Vasopressin promote the water uptake across the skin, water accumulated in the body steadly in the all seasons except summer season. 4. In summer, vasopressin upset the frog's water balance, even when it did suppress urine output.

양서류의 수분평형에 미치는 항이뇨 호르몬작용의 계절적 변동

성양호 고려대학교 의과대학 1974 고려대학교 의과대학 잡지 Vol.11 No.2

파킨슨병 모델 동물에서 GDNF 아데노바이러스 벡터를 이용한 유전자치료

성기한(Ki-Han Sung),윤인규(In-Gyu Yoon),정현실(Hyun-Sil Jung),김을선(Eul-Seon Kim),김현주(Hyun-Joo Kim),김양호(Yang-Ho Kim),윤 식(Sik Yoon),김봉선(Bong-Seon Kim),김재봉(Jae-Bong Kim),백선용(Sun-Yong Baek) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.6

6-OHDA를 이용하여 실험적으로 유발한 파킨슨병 모델 동물을 대상으로 유전자 치료 목적으로 GDNF 유전자를 포함 하는 아데노바이러스 벡터(Ad-GDNF)를 주사하여 행동 변화와 줄무늬체와 흑색질의 tyrosine hydroxylase (TH) 면역반응양성의 도파민성 세포의 형태학적 변화를 연구하였다. 성숙한 수컷 Sprague-Dawley계 흰쥐의 줄무늬체에 6-OHDA를 주사하고, 1주 후에 apomorphine에 의한 회전 운동 양상 을 측정하여 분당 7회 이상 회전하는 동물은 파킨슨병 모델 동물에 해당하는 것으로 하였다. 이들 동물의 줄무늬체에 Ad-GDNF를 주사하고 1주 후에 apomorphine에 의한 행동 양상을 다시 측정하고, 이어서 심장 관류고정을 실시하여 형태 학적 변화를 관찰하였다. Ad-GDNF 대신에 LacZ 유전자를 포함하는 아데노바이러스 벡터(Ad-LacZ)를 주사한 것을 대조 군으로 이용하였다. 1. 파킨슨병 모델 동물의 줄무늬체에 Ad-GDNF를 주사한 동물에서 apomorphine에 의한 회전 운동 수가 유의하게 감소 하였다. 그러나 Ad-LacZ를 주사한 대조군에서는 회전 운동 수의 변화를 관찰할 수 없었다. 2. 회전 운동 수가 감소한 동물의 줄무늬체에 존재하는 TH 면역반응 양성의 축삭 종말은 대조군의 75% 수준으로 Ad- LacZ를 주사한 37%에 비하여 현저히 증가하여 TH 면역반응 양성 축삭종말의 퇴행성 변화가 방지되었다. 3. 흑색질의 TH 면역반응 양성 신경세포의 수는 Ad-GDNF를 주사한 군에서는 대조군의 82% 수준으로 Ad-LacZ를 주사한 군의 60% 수준에 비하여 유의하게 증가되어 있어서 6-OHDA에 의한 신경세포의 사멸이 방지되는 것을 알 수 있었다. 이상의 결과로 미루어 파킨슨병 모델 흰쥐의 줄무늬체에 Ad-GDNF를 한차례 주사하여 1주 후에 줄무늬체와 흑색질의 도파민성 세포의 사멸이 방지되는 것을 확인하였다. A recombinant adenoviral vector encoding human GDNF gene (Ad-GDNF) was developed to investigate the effect of GDNF gene in 6-OHDA-induced Parkinson’s disease rat. The changes of rotatory behavior and density of THimmunoreactive axon terminals and number of cell bodies were observed by the GDNF expression. Adult male Sprague-Dawley rats were used. Parkinson’s disease (PD) rat models were prepared by the injection of 6-OHDA into the striatum. After 1 week, the animals showing apomorphine-induced rotatory behavior above 7 turns/ min were defined as PD rat model. Ad-GDNF was injected into the striatum of animal model and tested the apomorphine- induced rotatory behavior at 1 week after injection. These animals were perfused with Zamboni fixative to investigate the morphological changes after rotatory behavior test. Instead of Ad-GDNF, Ad-LacZ injected PD rats were used as a control. The following results are obtained: 1. The apomorphine-induced rotational behavior was significantly reduced by the treatment of PD rat by the injection of Ad-GDNF. The Ad-LacZ injected PD rat showed no change in rotatory behavior. 2. The density of TH-ir axon terminals in the striatum was significantly increased by the Ad-GDNF injection (75% of normal side), but there was no change in the density by the Ad-LacZ injection (37% of normal side) compared to 6-OHDA lesioned striatum. This means the Ad-GDNF injection prevented the degenerative change of TH-ir axon terminals in the stritum of the PD rat. 3. The number of TH-ir cell body was significantly recovered by the Ad-GDNF (82% of normal side), but there was not recovered by the Ad-LacZ injection (60% of normal side) compared to 6-OHDA lesion. This means the retrogradely transported Ad-GDNF induced the TH expression in the dopaminergic neurons of the substantia nigra. Gene therapy with Ad-GDNF prevented the degeneration of dopaminergic neurons and axon terminals in the 6- OHDA-induced PD rat.

산소분압 변동이 혈액 및 여러 장기에 미치는 영향 : Ⅰ.혈액성분, 조혈기능 및 심장에 대한 영향 1. The Effects on the Erythrocyte, Erythropoietin Activity and the Heart Metabolism

이계열,홍승길,신영철,유총근,성양호 최신의학사 1972 最新醫學 Vol.15 No.9

To investigate the effects of hypoxic and hyperoxic conditions on the erythrocyte, erythropoietin activity and heart metabolism, this experiment was performed to the animals of 24 rabbits and about 300 mice. Hypoxic condition was induced by flowing the gas mixtures which contain 8% oxygen and 92% nitrogen into a closed hyperbaric chamber, and hyperoxic condition by flowing the gas mixture containing 40% oxygen and 60% nitrogen for 5 hours per day during the period of 15 days respectively. The flow rates of each gas mixtures were corrected by analyzing the oxygen content of outlet gases with micro-Scholander apparatus. Blood samples were obtained from the ear vein with heparinized syringes at the end of one-week and two-week exposure for the detemination of RBC, hemoglobin, hematocrit and RBC fragility and also arterial blood serum was collected and stored in freezer for the measurement of erythropoietin activity. This serum was injected subcutaneously to the plethoric mice and determined 59Fe incorporation rate. Oxygen consumption and lactic acid level of left ventricle excised from the heart of sacrificed rabbit were determined. The results obtained were as follows: 1. The increase of RBC count was more prominent in hypoxic group than in hyperoxic group. 2. Hemoglobin and hematocrit were increased significantly in hypoxic group and decreased in hyper Toxic group. 3. MCV, MCHC and MCH were increased in hypoxic group and decreased in hyperoxic group representing macrocytic shift in hypoxia and microcytic shift in hyperoxia. 4. Erythrocyte fragility was decreased in hypoxic group and increased in hyperoxic group. 5. Erythropoietin activity was increased in hypoxic group significantly, and decreased in hyperoxic group. 6. Oxygen consumption arid lactic acid level were elevated in hypoxia and depressed in hyperoxic group.

한국인 기초 신진대사량의 계절에 따른 변동

이계열,지선호,홍승길,성양호,Lee, Kae-Yol,Chee, Sun-Ho,Hong, Seung-Kil,Sung, Yang-Ho 대한생리학회 1972 대한생리학회지 Vol.6 No.2

Contrary to most of European and American investigators failed to find out the seasonal variations of basal metabolic rate in man, Japanese and Korean investigators reported the increase in winter, decrease in summer season. But the causes of variation were not found clearly. To find out whether metabolic acclimatization to climate could be arise or not in human being, the basal metabolic rate was determined monthly for a period of one year in Airmen volunteers who live in Seoul, with 9 l Collins spirometer. The results obtained were as follows: 1. The average ambient temperature was lowest in February $(-5.88^{\circ}C)$ and highest in July $(27.34^{\circ}C)$. 2. Basal metabolic rate was lowest in June and highest in December showing seasonal variations. Interestingly, the increase of basal metabolic rate followed after the drop of ambient temperature below $0^{\circ}C$ (December) and the decrease followed after the elevation of ambient temperature from optimum to hot (June) or cold to warm (March). 3, Mean skinfold thickness increased in spring, decreased in winter. 4. These findings indicate that the basal metabolic rate of Korean reveals the seasonal variation affected by ambient temperature highly.

腹部戰傷 56例에 對한 臨床的 考察

郭贊浩(Kwak, Chan Ho),成陽鎬(Seong, Yang Ho),金漢中(Kim, Han Jung) 대한외과학회 1970 Annals of Surgical Treatment and Research Vol.12 No.6