http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
홀몬으로 처리된 쥐의 $C_{6}$ glioma 세포배양으로부터 분리된 낙산탈수소 효소 A-mRNA의 3'-말단의 2차 구조
배석철,이승기 한국미생물학회 1987 미생물학회지 Vol.25 No.2
Rat liver LDH A-cDNA has been isolated from a .lambda.gt11-rat lover cDNA library and partially characterized. The size of the isolated rat liver LDH A-cDNA if shown to be 1.6Kb and restriction enzyme sites for the rat liver LDH A-cDNA are also mapped. 682-nucleotide sequence coding for 3'-end of rat liver LDH A-cDNA has been analyzed and compared to the nucleotide sequence of the same region of rat $C_6$-glioma cell LDH A-cDNA which has been cloned from the hormonally stimulated cell cultures. The result shows that 177 nucleotide sequences coding for the C-terminal 59-amino acids are identical but 505 nucleotide sequences of 3'-nontranslated region of the two LSH A-cDNA exhibit characteristic differences in thier nucleotide sequences. Computer analysis for the folding structures for 3'-end 400 nucleotide sequences of the two LDH A-cDNA shows a possibility implying that the two LDH A-mRNAs isolated from different tissues of rats may have different half life and therefore their translational efficiency may be different. It has been previously demonstrated that isoproterenol stimulated rat $C_6$ -glioma cell cultures produce LDH A-mRNA showing 2 to 3-fold longer half life in comparison to that of noninduced LHD A-mRNA. The result therefore support for the idea that hormonally stimulated rat $C_6$-glioma cells may produce LDH A-mRNA containing different nucleotide sequences at the 3'-end nontranslated region by which the hormonally induced LDH A-mRNA could have more stable secondary mRAN structure in comparison to that of noninduced LDH A-mRNA.
배석한,정연철 한국항해항만학회 2003 한국항해항만학회지 Vol.27 No.2
해양에 기름이 유출되면 방제작업의 초기단계에서 기름의 이동 및 확산을 차단하기 위하여 오일 붐을 사용하는 것이 일반적이다. 그러나 오일 붐은 유속, 기름의 밀도와 점성, 수심, 기름의 양, 그리고 붐 홀수의 길이 등과 같은 여러 인자들에 의해 그 포획능력이 영향을 받는다. 본 연구에서는 이들 누유인자가 오일 붐의 포획과정에 어떠한 영향을 미치는지를 체계적으로 파악하기 위하여 컴퓨터 모델링을 수행하였다. 모델링을 위하여 CFD(computational fluid dynamics) 프로그램 중에서 가장 널리 알려진 Fluent를 이용하였으며 기존의 실험자료와 비교함으로써 모델링 결과를 검증하였다. 본 연구의 결과는 방제작업 책임자와 오일 붐 설계자에게 유용한 참고자료가 될 것으로 기대된다. When oil is spilled at sea, the oil boom is commonly used to tackle the movement and spreading of oil in an early stage of oil spill combat. But, the retaining capability of oil boom is affected by various factors, such as water velocity, viscosity and density of oil, water depth, oil volume and the length of boom draft. In this study, a computer modelling was peformed to investigate how these factors influence the oil retaining process. The Fluent, most popular one of many CFD(computational fluid dynamics) programs is chosen for modelling and modelling results were verified using the empirical data. It is expected that results of this study will be useful data for oil boom designer and oil spill response commander.
효율높은 cloning system을 통한 Rat Liver 전장 낙산탈수소효소 A-cDNA의 제조 및 분리동정
노옥경,배석철,이승기 대한약학회 1987 약학회지 Vol.31 No.2
It is still difficult and time consuming to obtain cDNA sequences that contain the entire nucleotide sequence of the corresponding mRNA. A rapid and high efficient cloning method to obtain full-length cDNA segments is thus developed. The cloning procedure described here consists of the construction of oligo(dT)-tailed vector primer using pWR34 plasmid, polyadenylation of mRNA-cDNA heteroduplex using terminal deoxytransferase, and replacement of MRNA strand with DNA by RNase H and DNA polymerase I. The restriction endonuclease analysis shows that the size of inserted-cDNA is in the range of 1.5~4.0 kb long suggesting that most of cloned cDNA are full-length or nearly full-length cDNA. The plasmid-DNA recombinants obtained were 4$\times$$10^5$~$10^{6}$ per $\mu\textrm{g}$ of rat liver poly (A$^+$)mRNA, which is 4 to 10 fold higher cloning efficiency in comparison to the presently used methods for full-length cDNA cloning. The results indicate that the described cloning system is much simpler, less time consuming, and very efficient cloning method to construct a cDNA library.
서장국,배석철 한국중원언어학회 2019 언어학연구 Vol.0 No.50
The purpose of this study is to clarify the musical characteristics of English and the common characteristics shared by music and English stress patterns and rhythm. For this purpose, we examined the stress patterns and rhythm of the basic English words and applied them to the analysis of English hymnal. The findings of our analysis showed that the stress patterns of the English words are very similar to the beat types of music. The basic rhythm of the English language is closely related to that of music and the two apparently different phenomena can be explained through the common principles of rhythm. Additionally, the concept of syllable extrametricality used in stress assignment of the English words turned out to be very similar to the application style and nature of the incomplete bar of music. Finally, it was confirmed that the stress patterns of the English words faithfully match the lyrics of the English hymns under the rhythm principle of music.