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박호란 ( Park Ho Lan ),송경애 ( Song Gyeong Ae ),김남초 ( Kim Nam Cho ),양수 ( Yang Su ),이소영 ( Lee So Yeong ),문영임 ( Mun Yeong Im ) 한국정신간호학회 2003 정신간호학회지 Vol.12 No.3
N/A Purpose: This study aimed to provide basic data for adolescent drinking prevention through the investigation of drinking status of middle school students. Method: Students from 15 different middle schools located in the S district of Seoul were investigated using a self questionnaire. A total 1,957 students, two classes of 7-8 graders from each school, answered the questionnaire during November 8 to 19, 2002. The tool used for the research was modified version of what Korean Association Against Drug Abuse and Korean Drinking Culture and Alcohol Research have developed and a subscale of POSIT used to identify middle schooler`s problem drinking. The Cronbach`s a of the tool was .87. The data analyzed using Spss Win 10.0. Results: 1. The rate of students who have experienced drinking was 64.1% and 23.1% are still drinking. 3.4% drinks more than 2 times a week. 2. A total 94% replied they have started drinking before 7th grade and 53.9% started during elementary school and 20.7% before elementary school and 19.4% during 7th grade. 3. The rate of middle schooler`s problem drinking measured by a subscale of POSIT was 5.5% among total subjects, and 8.5% among experienced drinkers. Conclusions: To prevent middle schooler`s drinking, a accurate survey of drinking should be carried out repeatedly and relating factors to middle schooler`s drinking be analyzed.
양수 ( Yang Su ),박호란 ( Park Ho Lan ),송경애 ( Song Gyeong Ae ),김남초 ( Kim Nam Cho ),조선진 ( Jo Seon Jin ),문정순 ( Mun Jeong Sun ) 한국정신간호학회 2003 정신간호학회지 Vol.12 No.3
N/A Purposes: This study was conducted to offer some scopes that is needed to develop an strategy for preventing of drinking in high school students. For this, we analysed the factors affecting on high school students` alcohol use. Methods: An survey was conducted from 5, Nov. 2002 to 11, Nov. 2002 to the high school students(1,551) of the 1st and 2nd grade at S-gu at Seoul based on the self-reported questionnaires. The data were analyzed by the SPSS for Windows 10.0 with multiple logistic regression to determine the factors affecting on aocohol use. Results: The drinking rate of life-time was 82.1%, and current drinking rate was 70.2%. Mother`s drinking(OR=1.89, P=.019), having drinking friend(s)(OR=3.74, P=.005), drinking alone or with friend(s) at the first experience(OR=1.87, p=.031), current smoker(OR=11.42, p=.02) increased the possibility of being current drinkers. Conclusions: With comprehensive regards to family, friend-school life, and health risk behavior factors, a program of drinking prevention for high school students should be developed.
꿀벌(Apis mellifera)의 장내 세균인 Bacillus sp. HY-20이 분비하는 Xylanase의 특성
이란희,김도영,한미경,오현우,함수진,박두상,배경숙,석대은,신동하,손광희,박호용,Lee, Lan-Hee,Kim, Do-Young,Han, Mi-Kyoung,Oh, Hyun-Woo,Ham, Su-Jin,Park, Doo-Sang,Bae, Kyung-Sook,Sok, Dai-Eun,Shin, Dong-Ha,Son, Kwang-Hee,Park, Ho-Yong 한국미생물학회 2009 미생물학회지 Vol.45 No.4
꿀벌(Apis mellifera)의 장으로부터 xylan분해능이 우수한 세균 HY-20을 분리하였고, Bacillus 속으로 동정하였다. 분리균주 HY-20의 세포 외 GH11 xylanase (XylP) 유전자 (687-bp)는 25,522 Da의 분자량과 9.33의 pI 값을 갖는 228개의 아미노산으로 구성된 단백질을 인코딩하는 것으로 확인되었으며, 지금까지 특성규명이 이루어지지 않은 B. pumilus xylanase (GenBank accession no.: AY526092)의 아미노산 서열과 97%의 상동성을 보였다. pET-28a(+)/xylP를 포함하고 있는 Escherichia coli BL21 균주에서 과발현 된 His-tagged XylP (rXylP)는 cation exchange 및 gel permeation chromatography를 통해 순수하게 분리 정제 되었으며, $50^{\circ}C$의 온도와 pH 6.5 조건에서 반응할 때 birchwood xylan에 대해 가장 높은 가수분해 활성을 나타내었다. rXylP는 15분간 $55^{\circ}C$에 노출될 때 본래 활성의 약 50%를 잃어버리는 전형적인 중온성 효소의 특성을 보였으며, $Hg^{2+}$ (1mM)와 N-bromosuccinimide (5mM)에 노출될 때 완전히 불활성화 되었고, $Mn^{2+}$ (1 mM), $Fe^{2+}$ (1mM) 및 sodium azide (5 mM)에 의해서는 활성이 약 10% 증가될 수 있는 것으로 밝혀졌다. 한편, rXylP는 xylose유래 다당류는 효율적으로 분해하였지만 PNP-sugar 유도체 및 glucose 유래 다당류는 분해하지 못하였다. A xylan-decomposing bacterium, HY-20, was isolated from the gut of a honeybee, Apis mellifera, and identified as Bacillus sp. The extracellular GH11 xylanase (XylP) gene (687-bp) of strain HY-20 encoded a protein of 228 amino acids with a deduced molecular mass of 25,522 Da and a calculated pI of 9.33. The primary structure of XylP was 97% identical to that of B. pumilus xylanase (GenBank accession no.: AY526092) that has not been characterized yet. The recombinant His-tagged enzyme (rXylP) overexpressed in Escherichia coli BL21 harboring pET-28a(+)/xylP was purified to electrophoretic homogeneity by cation exchange and gel permeation chromatographies. The purified enzyme exhibited the highest catalytic activity toward birchwood xylan at pH 6.5 and $50^{\circ}C$ and retained approximately 50% of its original activity when pre-incubated at $55^{\circ}C$ for 15 min. The recombinant enzyme was completely inactivated by $Hg^{2+}$ (1 mM) and N-bromosuccinimide (5 mM), while its activity was slightly stimulated by approximately 10% in the presence of $Mn^{2+}$ (1 mM), $Fe^{2+}$ (1 mM), and sodium azide (5 mM). rXylP was able to efficiently degrade various polymeric xylose-based substrates but PNP-sugar derivatives and glucose-based polymers were not susceptible to the enzyme.
김남초 ( Kim Nam Cho ),양수 ( Yang Su ),박호란 ( Park Ho Lan ),송경애 ( Song Gyeong Ae ),이소영 ( Lee So Yeong ),장혜원 ( Jang Hye Won ) 한국정신간호학회 2003 정신간호학회지 Vol.12 No.1
N/A Purpose: This study is to survey parents` recognition, attitude and educational need about adolescent drinking. Methods: This survey was conducted through questioning the parents of samples on the subjects from November 5th to 22th of 2002. Those are 3,493 students and 2,522 parents of the students of the 1st and 2nd grade, who were selected randomly among 26 middle and high schools in Socho-gu, Seoul. Results: The parents who had experienced drinking were 89.1% of the samples and the those who had drunk two times a week were 26% of them. The parents who knew that their children had experienced drunk were 28.8% of the samples of the samples, the 6.5% of them knew that their children were drinking then. The 93.6% of the samples thought that the education preventing their children from drinking was necessary. The parents who thought that the drinking preventive education was necessary, and who thought the adolescent drinking serious got the higher score than the parents who did not think as such. Conclusion: It is necessary that the education programs appropriate to the education need of the parents must be developed and conducted, In addition to that, we have to do further studies which could compare the variation of the parents` recognition and attitudes toward their children`s drinking after the execution of education program about adolescent drinking.
Methotrexate로 치료한 타카야수 동맥염에 동반된 괴저성 농피증
김종근 ( Jong Keun Kim ),서준혁 ( Jun Hyuk Seo ),윤준철 ( Jun Cheol Yoon ),박도회 ( Do Hwae Park ),조민재 ( Min Jae Jo ),이찬우 ( Chan Woo Lee ),백애란 ( Ae Lan Paik ),민복기 ( Pok Kee Min ),나건연 ( Gun Yoen Na ),오동호 ( Dong Ho 대한류마티스학회 2006 대한류마티스학회지 Vol.13 No.1
Pyoderma gangrenosum (PG) is a vasculitic process, often associated with systemic diseases, including Takayasu`s arteritis (TA), rheumatoid arthritis, and inflammatory bowel disease. Most cases of PG associated with TA have been observed in Japan. However, this association is not commonly observed in North American and European patients. PG is often refractory to therapy, requiring high dosages of glucocorticoids. Here, we describe a case of recalcitrant PG that was associated with TA and successfully treated with methotrexate.
교사의 음주관련 특성과 청소년 음주에 대한 인식, 태도 및 교육요구도
송경애 ( Song Gyeong Ae ),김남초 ( Kim Nam Cho ),양수 ( Yang Su ),박호란 ( Park Ho Lan ),이소영 ( Lee So Yeong ),유숙자 ( Yu Sug Ja ) 한국정신간호학회 2003 정신간호학회지 Vol.12 No.1
N/A Purpose: This study aimed to provide basic data for developing adolescent alcohol prevention program through the investigation of teacher`s recognition and attitude about alcohol use of adolescents. Method: 1,124 teachers working at middle and high schools located in Socho-gu, Seoul, were responded during rhe period from November 5 to 24 in 2002. Result: 1. For frequency of drinking, 39.9% of subjects responded to drink once a month, 15.2% drink more than two times a week. 2. For the amount of drinking, 37.0% drink less than 2 glasses while 12.8% drink over 10 glasses at a time. 3. Based on AUDIT score, drinker with more than 8 point from AUUDIT(Alcohol Use Disorder Identification Test) are 33.5%. Male are 30.7% while female are 2.72%. So social drinker group represents 66.5%. 4. Most of the subjects said education program for adolescent alcohol prevention is needed, but only 5.7% of them has counselled. 5. The score of teachers` recognition and attitude about alcohol use of adolescents was significantly higher in non current drinker group, female, middle school teacher than that of current drinker group, male, highschool teacher. It can be suggested that teachers` recognition and attitudes about adolescent drinking was important to prevent alcohol use of adolescent and teacher`s education program are needed for effective education. And we will have to further study in nationwide.