폐국균종의 임상적 고찰

이상훈 ( Sang Hoon Lee ),이병준 ( Byoung Jun Lee ),정도영 ( Do Young Jung ),김진희 ( Jin Hee Kim ),손동섭 ( Dong Suep Sohn ),신종욱 ( Jong Wook Shin ),김재열 ( Jae Yeol Kim ),박인원 ( In Won Park ),최병휘 ( Byoung Whui Choi ) 대한내과학회 2003 대한내과학회지 Vol.64 No.2

목적: 폐국균종은 만성 폐질환으로 인한 공동성 병소를 가진 환자들에서 대량 객혈과 같은 치명적인 증상을 야기할수도 있는 질환으로, 이에 저자들은 폐국균종으로 진단, 치료받은 36예의 환자들에서 주된 증상, 기저질환, 진단방법, 치료에 대하여 후향적으로 조사하였다. 방법: 1988년 2월부터 2000년 2월까지 중앙대 의료원에서 폐국균종으로 진단되어 치료받은 환자 36예를 대상으로 하였다. 26예는 조직학적으로 확진되었으며 나머지 10예는 흉부방사선학적 Background: Pulmonary aspergilloma usually results from the ingrowth of the colonized Aspergillus in the damaged bronchial tree, pulmonary cyst, or cavities of patients with underlying lung diseases. We analyzed the clinical features, diagnostic methods,

tRNA의 생합성에 관한 연구 : 효모 선구 tRNA의 Intervening Sequence의 Processing

김상희,이강렬,박인원,Kim, Sang-Hee,Lee, Kang-Ryul,Park, In-Won 생화학분자생물학회 1981 한국생화학회지 Vol.14 No.4

효모 균주 SUP 7 Mod II-3B에서 이차원 폴리아크릴아미드 겔 전기영동법으로 약 20개의 선구 tRNA를 분리했다. 이 선구 tRNA의 분자 크기는 5.8S와 4.5S RNA 크기 사이의 것으로서 모두 소수염기를 가지고 있었다. 이중 몇가지 선구 tRNA는 intervening sequence를 가지고 있음이 확인되었다. Intervening sequence를 가지고 있는 선구 tRNA를 S-30 분획과 핵분획으로 처리하면 tRNA의 분자 크기의 조각과 약 20개 정도의 누클레오티드의 작은 조각으로 절단 되었는데, 이것은 S-30 분획과 핵분획에 선구 tRNA의 intervening sequence를 절단하는 가공효소가 들어 있다는 가능성을 암시한다. Some 20 precursor tRNAs were isolated from yeast cell, SUP 7 Mod II-3B by two-dimensional polyacrylamide gel electrophoresis. These precursors were in the 4.5S~5.8S size range and contained already moidified miner bases at precursor level. Some of the precursor tRNAs were identified to have an intervening sequence in the anticodon region. The treatment of precursor tRNA with S-30 fraction and nuclei fraction gave rise to half-sized tRNA fragments and the fragments of about 20 nucleotides long. This result suggests that tRNA processing enzyme activity which removes the intervening sequence is present in the S-30 fraction as well as in the nuclei fraction.

tRNA 의 생합성에 관한 연구 효모 선구 tRNA 의 Intervening Sequence 의 Processing

김상희,이강렬,박인원 ( Sang Hee Kim,Kan Gryul Lee,In Won Park ) 생화학분자생물학회 1981 BMB Reports Vol.14 No.4

Some 20 precursor tRNAs were isolated from yeast cell, SUP 7 Mod II-3B by two-dimensional polyacrylamide gel electrophoresis. These precursors were in the 4.5 S∼5.8 S size range and contained already moidified miner bases at precursor level. Some of the precursor tRNAs were identified to have an intervening sequence in the anticodon region. The treatment of precursor tRNA with S-30 fraction and nuclei fraction gave rise to half-sized tRNA fragments and the fragments cf about 20 nucleotides long. This result suggests that tRNA processing enzyme activity which removes the intervening sequence is present in the S-30 fraction as well as in the nuclei fraction.

비소세포폐암 수술 후 세포분화도가 재발에 영향을 미친다

강형구 ( Hyung Koo Kang ),조성근 ( Sung Gun Cho ),이혜민 ( Hye Min Lee ),박성운 ( Sung Woon Park ),이병욱 ( Byung Ook Lee ),이재희 ( Jae Hee Lee ),김보민 ( Bo Min Kim ),박인원 ( In Won Park ) 대한결핵 및 호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.68 No.1

Background: Lung cancer is the most common cause of cancer mortality in Korea. The TNM stage at presentation in patients with non-small cell lung cancer (NSCLC) has the greatest impact on prognosis. Patients who undergo a complete resection for NSCLC are likely to develop recurrent and/or metastatic disease. There are several factors influencing the development of recurrence. We explored risk factors of recurrence in patients with stages I and II NSCLC, who had undergone curative resection. Methods: We reviewed patients who had complete surgical resection as definitive treatment for stage I or II. Patients followed up for more than 36 months. We evaluated several factors which might have relationship with recurrence, such as patient`s demographic factors, TNM staging, pathologic finding, tumor markers and surgical technique. Results: A total of 75 patients were enrolled for analysis, of whom 58 were men and 17 were women with mean age of 61 (range, 37 to 76) years. The average size of tumors was 3.9 cm (0.7 to 10 cm). There were 64 patients with stage I NSCLC and 11 with stage II NSCLC. Among 64 patients with stage I NSCLC, 35 patients showed recurrences whereas 8 patients have recurred in stage II NSCLC. Grade of differentiation of tumor was closely related to the recurrence. Seventy-five percent of patients who had poor tumor differentiation experienced a recurrence. In contrast, 3 patients of twelve had recurrences, who revealed differentiation in their tissue (p<0.05). Conclusion: Tumor differentiation could be a predictive factor for tumor recurrence in patients who have undergone curative resection for stage I or II NSCLC.

만성 신부전 환자의 혈액투석중 폐기능 변화에 관한 연구

이지훈(Jee Hoon Lee),김기영(Ki Young Kim),김성수(Sung Su Kim),박인원(In Won Park),강응택(Eung Taek Kang),최병휘(Byeung Whui Choi),허성호(Sung Ho Hue),유석희(Suk Hee Yu),박선규(Sun Kyu Park),서승천(Seung Chun Seo) 대한내과학회 1990 대한내과학회지 Vol.39 No.2

N/A Hypoxemia has occurred during the first 30-60 minutes of hemodialysis in patients with chronic renal failure who bad undergone hemodialysis regularly. It is primarily caused by hypoveniilation, secondary to carbon dioxide losses via dialysates and leukopenia associated with pulmonary vascular leukostasis which was induced by complement activation due to cuprophane membrane. However, there is still controversy over whether hypoxemia might be the result of bronchoconstriction during hemodialysis. To assess the relationship between hypoxemia and the changes of peripheral white blood cell counts and pulmonary function test when cuprophane membrane and acetate buffer solution had been used, arterial blood gas analysis, white blood cell counts, pulmonary function test during the first 120 minutes of hemodialysis were measured. The results were as follows: 1) The major falls of arterial blood oxygen tension occurred 15 minutes after the start of hemodialysis(p<0.05), and the biearbonate concentration significantly and gradually increased 30 minutes and 120 minutes after the start of hemodialysis(p<0.05). 2) The pulse rate significantly increased 15 minutes and 30minutes after the start of hemodialysis(p<0.05). 3) The arterial white blood cell counts and the precentage of neutrophil significantly decreased 15 minutes after the start of hemodialysis and then gradually recovered to a predialytic level(p<0.05), but the percentage of lymphocytes significantly increased 15 minutes after the start of hemodialysis and then gradually recovered to a predialytic level (p<0.05). 4) MMF, PEFR, and FEF75 significantly increased, but no significant changes of FEV1/FVC occurred during hemodialysis. Therefore, we suggest that acute hypoxemia may occur not due to bronchoconstriction, but due to pulmonary vascular leukostasis associated with complement activation.

백서의 급성폐손상에서 surfactant의 항염증작용과 호중구의 NK-${\kappa}B$ 활성과의 관계

안창혁,차영주,이경희,유철규,이병준,정도영,이훈,신종욱,김재열,박인원,최병휘,An, Chang-Hyeok,Cha, Young-Joo,Lee, Kyoung-Hee,Yoo, Chul-Gyu,Lee, Byoung-Jun,Jeong, Do-Young,Lee, Sang-Hoon,Shin, Jong-Wook,Kim, Jae-Yeol,Park, In-Won,Choi, 대한결핵및호흡기학회 2002 Tuberculosis and Respiratory Diseases Vol.53 No.5

연구배경 : 급성폐손상의 치료로 시도되는 표면활성물질의 효과는 허탈된 폐포를 재환기시키는 작용 외에 표면 활성물질 자제가 가지고 있는 항염증작용이 중요한 기전으로 생각되고 있다. 본 연구에서는 백서의 급성폐손상 모델을 이용하여 기관 내로 표면활성물질을 투여하였을 때, 기관지폐포세척액의 백혈구수와, 염증매개 사이토카인인 IL-$1{\beta}$ 그리고 IL-6의 농도에 변화가 있는지를 살펴보고, surfactant의 항염증작용이 전사인자인 NF-${\kappa}B$의 활성의 억제를 통하여 이루어지는지 여부를 Electrophoretic mobility shift assay (EMSA) 법으로 확인하였다. 방 법 : 대상동물은 300g 내외의 수컷 백서를 이용하였으며, 대상동물을 각각 6 마리씩 세 군으로 나누었다. 대조군은 기관 내로 생리식염수(3ml/kg)를 30분 간격으로 투여하였다. 나머지 두 군은 기관 내로 내독소(5mg/kg)를 투여하여 급성폐손상을 유발하고, 30 분 후에 표변활성 물질 치료군은 surfactant(30mg/kg)을 그리고 비치료군은 생리식염수(3ml/kg)을 각각 기관 내로 투여하였다. 생리식염수나 내독소를 투여한 24 시간 후에 기관지폐포세척술을 시행하였고, 기관지폐포세척액 내의 백혈구수와 IL-$1{\beta}$ 그리고 IL-6의 농도를 측정하였다. 또한 기관지폐포세척액에서 호중구를 분리하고 핵 단백질을 추출하여 NF-${\kappa}B$의 활성을 EMSA법으로 측정하였다. 결 과 : 대조군, 표면활성물질 치료군, 그리고 비치료군의 기관지폐포세척액의 백혈구 수는 각각 $356{\pm}275{\times}10^3/{\mu}1$, $3,221{\pm}1,914{\times}10^3/{\mu}1$, 그리고 $5,561{\pm}1,757{\times}10^3/{\mu}l$으로 비치료군의 백혈구 수가 가장 높았고, 다음으로 표면활성물질 치료군, 비치료군의 순서였다(p<0.05). 기관지폐포세척액의 IL-$1{\beta}$ 농도는 대조군은 0pg/ml, 표면활성물질 치료군은 $360{\pm}234pg/ml$, 그리고 비치료군은 $2,064{\pm}1,082pg/ml$로 대조군에 비해 표면활성물질 치료군이, 그리고 표면활성물질 치료군에 비해 비치료군의 IL-$1{\beta}$농도가 높았다(p<0.05). 기관지폐포세척액의 IL-6 농도는 대조군, 표면활성물질 치료군, 비치료군에서 각각 $49{\pm}62pg/ml$, $1,754{\pm}1,340pg/ml$, 그리고 $3,621{\pm}567pg/ml$으로 대조군에 비해 표면활성물질 치료군이, 그리고 표면활성물질 치료군에 비해 비치료군의 농도가 높았다(p<0.05). 표면활성물질 치료군과 비치료군 사이에서 호중구의 NF-${\kappa}B$ 활성화에는 차이가 없었다. 결 론 : 이상의 연구로 내독소의 기관 내 투여로 유발한 백서의 급성폐손상에서 기관 내로 투여한 표면활성물질은 기관지폐포세척액의 백혈구수와 염증매개 사이토카인인 IL-$1{\kappa}$ 그리고 IL-6의 농도를 감소시켜 폐포 내의 염증을 감소시켰으며, 표면활성 물질의 항염증작용은 NF-${\kappa}B$의 활성의 억제를 통하여 이루어지지는 않는 것으로 판단된다. Background : The therapeutic effects of surfactants on acute lung injury derive not only from their recruiting action on collapsed alveoli but also from their anti-inflammatory action in the alveolar sapce. This study evaluated the anti-inflammatory action of a surfactant in an acute lung injury model of rats by neutrophils were recollected from the BAL fluid and the NF-${\kappa}B$ activity of the neutrophilic nuclear protein was evaluated. Methods : Male Sprague-Dawley rats weighing approximately 300 gram were divided into 3 groups, which consisted of 6 rats respectively. In the control group, normal saline(3ml/kg) was instilled into the trachea twice with 30 minute interval. In two other groups, acute lung injury was induced by the intra-tracheal instillation of LPS(5mg/kg). Thirty minutes later, either a surfactant(ST group; 30mg/kg) or normal saline(NT group: 3ml/kg) was instilled via the trachea. Twenty-four hours after the LPS instillation, the BAL fluid was retrieved to measure the WBC count and cytokine(IL-$1{\beta}$ and IL-6) levels. The neutrophils were isolated from the BAL fluid and the nuclear protein was extracted to evaluate the NF-${\kappa}B$ activity using a eletrophoretic mobility shift assay(EMSA). Results : The WBC count of the BAL fluid of the ST group($3,221{\pm}1,914{\times}10^3/{\mu}l$) was higher than that of the control group($356{\pm}275{\times}10^3/{\mu}l$)(p<0.05) and lower than that of the NT group($5,561{\pm}1,757{\times}10^3/{\mu}l$)(p<0.05)). The BAL fluid level of IL-$1{\beta}$ from the NT group($2,064{\pm}1,082pg/ml$) was higher than those of the ST group($360{\pm}234pg/ml$)(p<0.05) and the control group(0pg/ml)p<0.05) and control group($49{\pm}62pg/ml$)(p<0.05). The NF-${\kappa}B$ activity of the neutrophilic nuclear protein in the ST group and NT group was similar. Conclusion : The surfactant, attenuates the alveolar inflammation in the acute lung injury of rats model. However, its anti-inflammatory action does no't appear to be mediated by the inhibition of NF-${\kappa}B$ activity.