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tRNA 의 생합성에 관한 연구 효모 선구 tRNA 의 Intervening Sequence 의 Processing
김상희,이강렬,박인원 ( Sang Hee Kim,Kan Gryul Lee,In Won Park ) 생화학분자생물학회 1981 BMB Reports Vol.14 No.4
Some 20 precursor tRNAs were isolated from yeast cell, SUP 7 Mod II-3B by two-dimensional polyacrylamide gel electrophoresis. These precursors were in the 4.5 S∼5.8 S size range and contained already moidified miner bases at precursor level. Some of the precursor tRNAs were identified to have an intervening sequence in the anticodon region. The treatment of precursor tRNA with S-30 fraction and nuclei fraction gave rise to half-sized tRNA fragments and the fragments cf about 20 nucleotides long. This result suggests that tRNA processing enzyme activity which removes the intervening sequence is present in the S-30 fraction as well as in the nuclei fraction.
박인원,이강렬,남정이 ( In Won Park,Kan Gryul Lee,Jeong Ee Nam ) 생화학분자생물학회 1974 BMB Reports Vol.7 No.1
At 37℃, phenylglyoxalation of yeast tRNA proceeds for two hours and no further reaction could be detected. Number of guanine residues modified after two hours` phenylglyoxalation is about six moles per tRNA molecule. Twentyfour hours` reaction produces at most seven residues of modified guanine. As the extent of tRNA modification increased, amino acid acceptor activity of phenylalanine tRNA decreased. Since the phenylglyoxal modifies tRNA`s no more than six residues of their guanine residues after two hours` reaction, phenylglyoxalation might be a recommendable means for the modification of exposed guanine residues on tRNA molecules.
박인원,이윤배,이강렬 ( In Won Park,Yun Bae Lee,Kan Gryul Lee ) 생화학분자생물학회 1973 BMB Reports Vol.6 No.1
Three reaction products were obtained by the reaction between cytosine and formaldehyde at pH 5.6. The products formed were separated by using Dowex AG 50W-X4 column, 2 ㎝ × 36 ㎝. The column was eluted with 2 N HCl at a flow rate of 1 ㎖/min.. From the nitrous acid treatment of the products, we conclude the structures to be as following; the monomethylol derivative is modified at 1-N position, and other two products are methylene bis-derivatives, one of which is bridged at 1-N positions of two cytosine residues and the second one is bridged at N⁴ positions of two cytosine residues. The ultraviolet absorption of the bis-derivatives behaves as it is additively contributed by the two cytosine residues. All three products are stable in 2 N HCl solution, and relatively unstable in alkaline solution.
효모 tRNA 와 tRNA 선구체들의 변형된 누클레오시드
박혜옥,강석찬,이강렬,박인원 ( Hay Oak Park,Seock Chan Kang,Kan Gryul Lee,In Won Park ) 생화학분자생물학회 1983 BMB Reports Vol.16 No.4
Yeast temperature-sensitive mutant cells (ts 136) accumulate 10 precursor tRNAs when they are grown at nonpermissive temperasure (at 35℃). Pre-tRNA₃^(Leu) and pre-tRNA^(Ile)_(AUA) were identified by fingerprinting. Both tRNA precursors are mature at 5` and 3` ends, and have intervening sequences. Both precursors have the same modified nucleosides such as pseudouridine (Ψ), dihydrouridine (D), ribothymidine (T), and some modified cytidine, and modified guanosine. However, the molar yields of modification are less than unity, which indicates that the modification on the precursor level is still partial. and several other modified nucleosides which are present in mature tRNAs, are not found in the precursor tRNAs. Neither the modified adenosine nor inosine was detected in the precursor tRNA₃^(Leu) and precursor tRNA^(Ile)_(AUA).
Pseudomons testosteroni 의 5S rRNA 의 일차구조 및 이차구조
고문주,박인원,이강렬 ( Moon Joo Koh,In Won Park,Kan Gryul Lee ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.4
The primary structure of 5S rRNA from Pseudomonas testosteroni was determined by enzymatic and chemical methods. The 5S rRNA consists of 116 nucleotides and contains no modified nucleoside. The 5S rRNA has somewhat low homology (75%-79%) compared with those of the four other Pseudomonas species studied to date. Data on the cleavage sites by nuclease S1, RNase T1 and RNase V1 suggest that the molecule of 5S rRNA is rather flexible.
변형된 누클레오티드에 의한 변형된 핵산의 생합성 ( 1 ) 시티딜산과 구아닐산의 포름알데히드에 의한 메틸렌 비스 유도체의 형성
장세희,박인원,이강렬,우상범 ( Se Ihi Chang,In Won Park,Kan Gryul Lee,San Gbum Woo ) 생화학분자생물학회 1975 BMB Reports Vol.8 No.2
A methylene bis compound was formed by the reaction between guanylic acid, cytidylic acid and formaldehyde at pH 5.4, 25℃ after 10 to 15 days reaction. The products were separated on PEI-cellulose thin layer. The molar ratio in the resulting derivatives of guanine residue, cytosine residue and ^(14)C-HCHO was 1 : 1 : 1. 29. The U.V. spectrum of the bis derivative coincides perfectly with the composite spectrum obtained from the equimolar mixture of guanylic acid and cytidylic acid.