親和性 및 非親和性菌系의 混合 및 前後接種이 벼 稻熱病의 病斑擴大에 미치는 影響

朴瑞基,金基淸 한국응용곤충학회 1983 한국응용곤충학회지 Vol.22 No.4

Effect on blast lesion development was investigated in rice cultivar Milyang 23 and Jinheung using two races of Pyricularia oryzae compatible or incompatible to each of two rice cultivars. Discase lesions were measured in rice when inoculated with inoculum mixed with compatible and incompatible races, inoculated with a compatible race following with an incompatible race and vice versa. Lesions were smaller when rice was incoulated with an incompatible race and then inoculated with a compatible race than those inoculated in the reverse way both at the inoculated site and at 3cm-upper part from inoculated spot. The results suggested that induced resistance might play a role in suppression of lesion development although such an effect appeared to be influenced by heavy application of nitrogen fertilizer.

빅데이터 보안 분야의 연구동향 분석

박서기,황경태 한국정보화진흥원 2016 정보화정책 Vol.23 No.1

The purpose of the study is to analyze the existing literature and to suggest future researchdirections in the big data security area. This study identifies 62 research articles and analyses theirpublication year, publication media, general research approach, specific research method, and research topic. According to the results of the analyses, big data security research is at its intial stage in which non-empiricalstudies and research dealing with technical issues are dominant. From the research topic perspective, the areademonstrates the signs of initial research stage in which proportion of the macro studies dealing with overallissues is far higher than the micro ones covering specific implementation methods and sectoral issues. A fewpromising topics for future research include overarching framework on big data security, big data securitymethods for different industries, and government policies on big data security. Currently, the big data securityarea does not have sufficient research results. In the future, studies covering various topics in big data securityfrom multiple perspectives are anticipated. 본 연구의 목적은 빅데이터 보안 분야의 기존 연구를 분석하고, 향후 연구 방향을 모색하는 것이다. 이를 위해 국내외의 총62편의 논문을 식별하여, 발간년도, 게재 매체, 전반적인 연구접근 방법, 세부적 연구 방법, 연구 주제 등을 분석하였다. 분석 결과, 빅데이터 보안 연구는 매우 초기 단계로서, 비실증 연구가 압도적인 비중을 차지하고 있고, 관련 개념/기법에 대한 이해를 해나가는 과정으로서 기술-관리-통합의 단계로 진화한 정보보안 분야의 연구 동향에 동조하여 기술적인연구가 주로 진행되고 있다. 연구 주제 측면에서도 빅데이터 보안에 대한 전반적인 이슈를 다룬 총론적인 연구들이 보안 구현 방법론, 분야별 이슈 등의 각론적 연구에 비해 높은 비중을 나타내는 등 초기 단계의 모습을 나타내고 있다. 향후 유망한 연구 분야로는 빅데이터 보안에 대한 전반적인 프레임워크 수립, 업종별 빅데이터 보안에 대한 연구, 빅데이터 보안 관련 정부 정책 분석 등을 들 수 있다. 빅데이터 보안 분야의 연구는 본격적으로 시작된 지 얼마 되지 않아, 연구 결과가 상대적으로 매우 부족한편이다. 앞으로 다양한 관점에서 빅데이터 보안과 관련해 풍부한 주제를 다루는 연구가 진행되기를 기대한다.

모잘록병(Rhizoctonia solani)의 억제에 있어서 Chromobacterium violaceum이 생산하는 Chitinase의 역할

박서기,이효연,김기청 한국식물병리학회 1995 Plant Pathology Journal Vol.11 No.4

Draft Genome Sequence of a Chitinase-producing Biocontrol Bacterium Serratia sp. C-1

박서기,김영철 한국식물병리학회 2015 식물병연구 Vol.21 No.3

The chitinase-producing bacterial strain C-1 is one of the key chitinase-producing biocontrol agents used for effective bioformulations for biological control. These bioformulations are mixed cultures of various chitinolytic bacteria. However, the precise identification, biocontrol activity, and the underlying mechanisms of the strain C-1 have not been investigated so far. Therefore, we evaluated in planta biocontrol efficacies of C-1 and determined the draft genome sequence of the strain in this study. The bacterial C-1 strain was identified as a novel Serratia sp. by a phylogenic analysis of its 16S rRNA sequence. The Serratia sp. C-1 bacterial cultures showed strong in planta biocontrol efficacies against some major phytopathogenic fungal diseases. The draft genome sequence of Serratia sp. C-1 indicated that the C-1 strain is a novel strain harboring a subset of genes that may be involved in its biocontrol activities.

Pseudomonas fluorescens 에 의한 가지와 고추 모잘록병의 억제

박서기,박기범,김태민,김기청 順天大學校 農業科學硏究所 1994 農業科學硏究 Vol.8 No.-

Inhibition of Rhizoctonia solani growth and the disease suppression were investigated with bacteria isolated form the rhizosphere soils of eggplant and hotpeper grown in vinylhouse in order to obtain antagonistic rhizobacteria which can be used to biocontrol damping-off of eggplant and hotpepper. An isolate S-2, which showed the highest activity in inhibition of R. solani mycelial growth and suppression of the disease, were indentified as Pseudomonas fluorescens Biovar Ⅲ. The activity of strain S-2 on inhibition of mycelial growth on KM-PDA plates was high against R. solani, but low against Fusarium oxysporum and Phytophthora capsici. Strain S-2 was resistant to ampicillin and nalidixic acid at 200㎍/ml. In twenty days after strains S-2 treatment, the diseased plants were 1.8 and 10.3% in eggplant and hotpepers, respectively, as compared with both 100 % in nontreated ones. Strain S-2 was also much better in its ability on the disease suppression than that of P. fluorescens (Pf-5) and P. aureofaciens (ATCC 13985) which have been shown to seppress damping-off caused by R. solani. Density of strain S-2 population in treated soils was not significantly changed until 26 days after treatment, and not different from those of Pf-5 and ATCC 13985.

CALS KOREA '95 스케치

박서기,Park, Seo-Gi 한국정보통신집흥협회 1995 정보화사회 Vol.95 No.-

Rhizoctonia solani 길항세균 Pseudomonas fluorescens의 Tn5 삽입 돌연변이주 분리 및 특성

박서기,박기범,김기청 한국식물병리학회 1994 Plant Pathology Journal Vol.10 No.1

Laccase/mediator system에 의한 크라프트 펄프 표백(제1보)-LKY-7 Laccase의 크라프트펄프 표백을 위한 mediator 선발-

박서기,김훈,정현채 한국펄프.종이工學會 2006 펄프.종이기술 Vol.38 No.3

땅콩 갈색무늬病과 흑색무늬病의 發生生態 및 品種抵抗性에 關한 硏究

朴瑞基 順天大學校 農業科學硏究所 1988 農業科學硏究 Vol.2 No.-

Several characters of brown leaf spot and black spot, which bring about marked decrease in yield of peanut, and resistance of varieties recommended in Korea were investigated in 1987-1988. Brown leaf spot and black spot began to occur in late May∼early June and in late July∼early August, respectively. Cercospora arachidicola produces circular to irregular, 1 to 10㎜ in diameter, dark brown to reddish brown on the upper sides, lighter brown on the below sides and usually surrounded by a yellow halo. Cercosporidium personatum produces mostly circular, 1 to 7㎜ in diameter, black brown on both sides and often surrounded by a yellow halo. Conidiophores of C. arachidicola are mostly epiphyllous, geniculate, 17.2-58.6×3.5-5.1㎛ and yellowish brown, and conidia are colourless to slightly olivaceous, clavate, 34.6-115.7×2.6-5.3㎛ and 3 to 15 septate. Conidiophores of C. personatum are mostly hypophyllous, geniculate, 23.5-71.6×4.1-6.4㎛ and yellowish brown, and conidia are mostly pale brown to olivaceous, mostly cylindrical to clavate, 28.3-59.7×4.9-9.8㎛ and 3-7 septate. Resistant varieties of peanut to C. arachidicola were Sinpung and Saedl, but those to C. personatum were 01 and Yeongho among varieties recommended in Korea.

The Biocontrol Activity of Chromobacterium sp. Strain C-61 against Rhizoctonia solani Depends on the Productive Ability of Chitinase

박서기,Myung-Chul Lee,Gary E. Harman 한국식물병리학회 2005 Plant Pathology Journal Vol.21 No.3

A chitinolytic bacterium, Chromobacterium sp. strain C-61, was found strongly antagonistic to Rhizoctonia solani, a causal agent of damping-off of eggplant. In this study, the biocontrol activity and enzymatic characteristics of strain C-61 were compared with its four Tn5 insertion mutants (C61-A, -B, -C, and -D) that had lower chitinolytic ability. The chitinase activity of a 2-day old culture was about 76%, 49% and 6% level in C61-A, C61-B and in C61-C, respectively, compared with that of strain C-61. The β-N-acetylhexosaminidase (Nahase) activity was little detected in strain C-61 but increased largely in C-61A, C61-B and C61-C.Activities of chitinase and Nahase appeared to be negatively correlated in these strains. Another mutant, C-61D, produced no detectable extracellular chitinase and Nahase. The in vitro and in vivo biocontrol activities of strain C-61 and its mutants were closely related to their ability to produce chitinase but not Nahase. No significant differences in population densities between strain C-61 and its mutants were observed in soil around eggplant roots. The results of SDS-PAGE and isoelectrofocusing showed that a major chitinase of strain C-61 is 54-kDa with pI of approximately 8.5. This study provides evidence that the biocontrol activity of Chromobacterium sp. strain C-61 against Rhizoctonia solani depends on the ability to produce chitinase with molecular weight of 54-kDa and pI of 8.5.