Inhibition of Rhizoctonia solani growth and the disease suppression were investigated with bacteria isolated form the rhizosphere soils of eggplant and hotpeper grown in vinylhouse in order to obtain antagonistic rhizobacteria which can be used to bio...
Inhibition of Rhizoctonia solani growth and the disease suppression were investigated with bacteria isolated form the rhizosphere soils of eggplant and hotpeper grown in vinylhouse in order to obtain antagonistic rhizobacteria which can be used to biocontrol damping-off of eggplant and hotpepper. An isolate S-2, which showed the highest activity in inhibition of R. solani mycelial growth and suppression of the disease, were indentified as Pseudomonas fluorescens Biovar Ⅲ. The activity of strain S-2 on inhibition of mycelial growth on KM-PDA plates was high against R. solani, but low against Fusarium oxysporum and Phytophthora capsici. Strain S-2 was resistant to ampicillin and nalidixic acid at 200㎍/ml. In twenty days after strains S-2 treatment, the diseased plants were 1.8 and 10.3% in eggplant and hotpepers, respectively, as compared with both 100 % in nontreated ones. Strain S-2 was also much better in its ability on the disease suppression than that of P. fluorescens (Pf-5) and P. aureofaciens (ATCC 13985) which have been shown to seppress damping-off caused by R. solani. Density of strain S-2 population in treated soils was not significantly changed until 26 days after treatment, and not different from those of Pf-5 and ATCC 13985.