Development of immunoassay using novel human papapillomavirus 16 E7 antibody

류기성 ( Ki Sung Ryu ),( Keun Ho Lee ),( Hee Jung Lee ),( Chan Joo Kim ),( Heun Soo Kang ),( Jong Sup Park ) 대한산부인과학회 2004 대한산부인과학회 학술대회 Vol.90 No.-

난소암 세포주에서 Mullerian Inhibiting Substance의 증식 억제 효과

류기성 ( Ki Sung Ryu ),서미영 ( Mi Young Seo ),조윤성 ( Yun Sung Jo ),김미란 ( Mee Ran Kim ),김진우 ( Jin Woo Kim ),한구택 ( Goo Taik Han ),이준모 ( Joon Mo Lee ),김장흡 ( Jang Heub Kim ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.11

Objective: In order to explore Mullerian inhibiting substance (MIS) effects on the ovarian neoplasia, the expression and localization of the MIS type II receptor (MISR II), the growth inhibitory effects of MIS, and the underlying molecular mechanisms were investigated in the ovarian cancer cell lines. Methods: Expression of MISR Ⅱ were studied in SKOV-3, OVCAR-3, and OVCAR-8 cell lines by immunohistochemical staining. The antiproliferative effects of MIS in these cell lines were investigated by methylthiazoletetrazolium (MTT) assay, fluorescence-activated cell sorting (FACS) analysis, annexin-V-FITC binding, and western blot analysis. Results: All cell lines showed strong specific staining for MISR II, although staining in OVCAR-8 cells was more intense than that in SKOV-3 and OVCAR-3. Treatment of OVCAR-8 cells with MIS led to a dose- and time-dependent inhibition of cell growth and survival was determined use by MTT assay. But OVCAR-3 cells exhibited growth inhibition at higher doses after 48 hours of treatment and SKOV-3 cells did not demonstrate response. Using FACS analysis, exposure of OVCAR-8 cells to MIS (71 nM) resulted in G1 arrest after 24 hours of treatment. This pattern was changed by time-dependent increase in the percentage of cells with a sub G0G1 DNA content, suggesting apoptosis, after 48 hours of treatment. These results suggested that cell death be preceded by cell cycle arrest. Time-related induction of apoptosis was also observed in this cell line as measured by annexin-V-FITC binding. In OVCAR-8 cells, the growth inhibitory effects of MIS were mediated through specific induction of CDKI p16 protein expression and via regulation of E2F1 in the absence of detectable levels of pRb. We estimated that OVCAR-3 cells were affected by MIS through p16-independent, alternative mechanistic pathways, since the growth inhibitory effects of MIS were minimal. SKOV-3 cells did not express p16 protein. Conclusion: We have demonstrated that ovarian cancer cells express the MISR II. Epithelial ovarian cancer cells respond to MIS by growth inhibition. Although the precise mechanisms of MIS mediated inhibition of ovarian cancer cell growth have not been fully defined, these data suggest that MIS has activity against ovarian cancers in vitro and may also be an effective targeted therapy for ovarian cancer.

항암제를 투여한 세포주의 배양액에서 M30을 이용한 고사의 측정

박상호 ( Sang Ho Park ),곽현성 ( Hyun Sung Kwack ),류기성 ( Ki Sung Ryu ),이영 ( Young Lee ),한구택 ( Ku Taek Han ) 대한산부인과학회 2010 Obstetrics & Gynecology Science Vol.53 No.5

Objective: We investigated a possible use of the induced apoptosis as a biomarker in the cells and their media treated with commonly used anti-cancer agents in gynecologic malignancies. Methods: After treatments with low and high concentrations of paclitaxel, cisplatin, and camptothecin in HeLa and OVCAR-3 cells, the levels of M30 antigen were detected in the cells and their media by immunofluorescence staining and ELISA methods, respectively. Results: The percentages of M30-fluoresein isothiocyanate (FITC) positive cells in HeLa and OVCAR-3 cells treated with paclitaxel, cisplatin, and camptothecin were 4.3% vs 18.1% vs 34.87% and 4.07% vs 18.6% vs 32.63%, 4.3% vs 17.87% vs 32.38% and 4.07% vs 16.83% vs 32%, and 4.3% vs 16.75% vs 31.3% and 4.07% vs 15.18% vs 29.9% in control, low dose, and hight dose groups, respectively (P<0.001). M30 antigen levels (U/L) measured in culture media of HeLa and OVCAR-3 cells treated with paclitaxel, cisplatin, and camptothecin were 53.03 vs 101.53 vs 355.59 and 86 vs 114.41 vs 412.04, 53.03 vs 79.84 vs 327.64 and 86 vs 125.44 vs 385.09, and 53.03 vs 88.41 vs 295.005 and 86 vs 108.42 vs 263.1 in control, low dose, and hight dose groups, respectively (P<0.001). Conclusion: Our results obtained in this preclinical study suggests that measurement of the levels of M30 antigen may help to predict the clinical responses and to select the effective anti-cancer agents in clinical settings, rapidly and quantitatively.

결절성 경화증이 동반된 태아 심장 횡문근종

길기철 ( Ki Cheol Kil ),남선영 ( Sun Young Nam ),김수미 ( Su Mi Kim ),하재은 ( Jae Eun Ha ),류기성 ( Ki Sung Ryu ),이영 ( Young Lee ) 대한주산의학회 2009 Perinatology Vol.20 No.4

Fetal cardiac tumors are a rare condition and are histologically benign. Cardiac rabdomyoma is the most common cardiac tumor and may be associated with tuberous sclerosis. Clinical follow-up including monitoring of fetal well-being is warranted as long as the fetus remains asymptomatic and there is no evidence for hydrops fetalis. If obstruction to blood flow and/or early fetal compromise is noted, then the decision of whether to deliver early must be made. We report a case of fetal cardiac tumor diagnosed by antenatal ultrasonography and diagnosed as tuberous sclerosis with brain MRI scanning after birth.

펜타센 TFT의 Flexible OLED 구동

최기범(Ki Beom Choe),류기성(Gi Seong Ryu),변현숙(Hyun Sook Byun),허영헌(Y. X. X),황성범(Sung Beom Hwang),송정근(Chun Kun Song) 제어로봇시스템학회 2004 제어로봇시스템학회 합동학술대회 논문집 Vol.2004 No.12

대음순에서 발생한 점액성 신경섬유종

곽현성 ( Hyun Sung Kwack ),박호정 ( Ho Jung Park ),임선옥 ( Sun Ok Im ),류기성 ( Ki Sung Ryu ),한구택 ( Ku Taek Han ) 대한산부인과학회 2009 Obstetrics & Gynecology Science Vol.52 No.1

Myxoid neurofibroma is a benign tumor of perineural cell origin, which is demonstrated with a positive immunohistochemical stainig for S-100. The most common locations of the myxoid neurofibroma are face, shoulder, arm, periungual and foot. We experienced an unusual case of myxoid neurofibroma which originated from labia majora area then reported it. To our knowledge, this is the first time that a labia majora location is reported.

DES (Diethyl stilbestrol)에 노출된 경험이 없는 27세 여성에서 발생한 자궁경부 투명세포암 및 근치적자궁경부절제술 (Radical Trachelectomy)

곽현성 ( Hyun Sung Kwack ),문영주 ( Young Joo Mun ),하재은 ( Jae Eun Ha ),황선정 ( Sun Jung Hwang ),김수미 ( Su Mi Kim ),한구택 ( Ku Taek Han ),류기성 ( Ki Sung Ryu ) 대한산부인과학회 2009 Obstetrics & Gynecology Science Vol.52 No.9

Clear cell adenocarcinoma (CCAC) is a rare cancer that comprises less than 9% of the cervical adenocarcinoma cases. We experienced a case of fertility-sparing radical abdominal trachelectomy for cervical clear cell adenocarcinoma (CCAC). Thus, reported it. A 27 year old female was diagnosed with clinical stage Ib cervical CCAC. She had no history of maternal exposure to diethylstilbestrol and had negative PAP cytology and HPV tests. She was treated with neoadjuvant chemotherapy followed by radical abdominal trachelectomy. After 2 cycles of postoperative adjuvant chemotherapy, the lesion disappeared completely in an imaging study, and potential fertility was preserved. Radical abdominal trachelectomy with chemotherapy may be a valuable approach for treating stage Ib cervical CCAC in women that wish to preserve potential fertility.

임신중 골반 기저를 뚫고 질벽으로 탈출한 장막하근종

조윤성 ( Yun Sung Jo ),권지영 ( Ji Young Kwon ),임희순 ( Hee Sun Lim ),문영주 ( Young Joo Mun ),이상형 ( Sang Hyoung Lee ),제동성 ( Dong Sung Jae ),한구택 ( Gu Taek Han ),류기성 ( Ki Sung Ryu ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.12

Myoma is the most common tumor in gynecologic field. As ultrasonography because popular in antenatal care, the more cases of myoma and those adverse effects during pregnancy are more frequently detected. The management of myoma during pregnancy is conservative, but in rare circumstances, surgical intervention including myomectomy may be required. We have experienced a case of protruded subserosal myoma with the uterine cervix in midtrimester of pregnancy. The patient was managed surgically by transvaginal myomectomy and had successfully maintained pregnancy. We report a case of protruded subserosal myoma through pelvic floor in pregnancy with brief review of literatures.

난소 종양에서 Mullerian Inhibiting Substance와 그 수용체의 발현

서경아 ( Kyoung A Seo ),류기성 ( Ki Sung Ryu ),이정원 ( Chung Won Lee ),최미나 ( Mi Na Choi ),차정호 ( Jung Ho Cha ),김장흡 ( Jang Heub Kim ),한구택 ( Ku Taek Han ) 대한산부인과학회 2005 Obstetrics & Gynecology Science Vol.48 No.2

Mullerian inhibiting substance (MIS) is a glycoprotein hormone produced by fetal Sertoli cells that causes regression of the Mullerian ducts in males during sexual differentiation. Cell lines derived from human ovarian epithelium and rodent Leydig cell tu

항암제를 투여한 HeLa와 OVCAR-3 세포주에서 Cytokeratin 18-Asp(396) (M30) 항체를 이용한 항암제 감수성의 측정

송민경 ( Min Kyung Song ),박상호 ( Sang Ho Park ),곽현성 ( Hyun Sung Kwack ),류기성 ( Ki Sung Ryu ),한구택 ( Ku Taek Han ) 대한산부인과학회 2010 Obstetrics & Gynecology Science Vol.53 No.1

Objective: The aim of this study was to detect the levels of M30-antigens as a biomarker of apoptosis in cells and their culture media after treatments with anticancer drugs as a preclinical study. Methods: After HeLa and OVCAR-3 cells were treated respectively with paclitaxel, cisplatin, and camptothecin, the harvested cells were stained sequentially with M30 monoclonal antibodies and propidium iodide (PI). Afterwards, they were analyzed using a FACScan flow cytometer and observed under an immunofluorescence microscope for M30-FITC immunofluorescences. Levels of M30 antigens were also detected in their culture media using M30-Apoptosense ELISA kit. Results: The levels of M30-FITC immunofluorescences were elevated in both cell lines after each drug treatments compared with those of control cells. The levels of M30 antigens detected by ELISA in media culturing each cell line treated with each of drugs were elevated compared with those of control cells. Conclusion: This study suggests that M30-antigens representing chemotherapy induced apoptosis may be a useful biomarker for predicting and monitoring the response of neoadjuvant chemotherapy in patients with gynecologic cancers.