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Brucella abortus 국내 분리주의 Heat Shock Protein 암호 groE 유전자의 염기서열 분석과 발현
김태용,김지영,장경수,김명철,박창식,한홍율,전무형,Kim, Tae-Yong,Kim, Ji-Young,Chang, Kyung-Soo,Kim, Myung-Cheol,Park, Chang-Sik,Han, Hong-Ryul,Jun, Moo-Hyung 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.1
GroE that is a heat shock protein composed of GroEL and GroES is known as an immunodominant target of both the humoral and cellular immune responses in bovine brucellosis. This study was carried out to characterize groE gene encoding heat shock proteins of B. abortus isolated in Korea and to evaluate the immunogenicity of the GroE protein expressed in E. coli system. In PCR the specific signals with the size of 2,077 bp were detected in five strains isolated from the mammary lymphnodes of the dairy cattle that were serologically positive and the reference strains. In comparison of the sequences of nucleotides and amino acids among the strains, GroES showed 100% identity in both sequences. GroEL was evaluated 99.0~99.9% in nucleotides and 98.0~100% homology in amino acids. The groE gene including groES and groEL was inserted into pET29a vector and constructed pET29a-GroE recombinant plasmids. The inserted groE was confirmed by digestion with Nco1 and EcoR1 endonucleases and nucleotide sequencing. E. coli BL (DE3) was transformed with pET29a-GroE, named as E. coli BL (DE3)/pET29a-GroE. In SDS-PAGE, it was evident that the recombinant plasmid effectively expressed the polypeptides for GroES (10 kDa) and GroEL (60 kDa) in 0.5, 1 and 2 hours after IPTG induction. The immuno-reactivity of the expressed proteins were proved in mouse inoculation and Western blot analysis.
다단계 온도 감지막을 가진 마이크로 흐름센서의 열전달 특성
김태용,정완영,Kim Tae Yong,Chung Wan-Young 한국정보통신학회 2005 한국정보통신학회논문지 Vol.9 No.3
마이크로 흐름센서는 반도체 집적기술을 이용할 수 있어, 소형으로 제작이 가능하며 빠른 응답특성을 가져 다양한 분야에 응용되고 있다. 본 연구에서는 넓은 흐름의 세기 영역에서 정밀한 감도를 가지는 2차원의 마이크로 흐름센서를 실리콘 기판위에 적충시켜 제작하였다. 흐름센서의 중앙에 하나의 히터와 양측에 온도를 검출할 수 있는 3쌍의 감지막을 가진 새로운 센서 구조를 제안하고, 온도 검출에 대한 성능평가를 위하여 시간영역에서의 유한차분법을 이용하여 공기흐름의 세기 변화에 따른 온도분포를 계산하였다. 계산결과를 통하여 실제 흐름센서의 동작을 정량적으로 분석하였다. A micro flow sensor on silicon substrate allows the fabrication of small components where many different functions can be integrated so that the functionality of the sensors can be increased. Further more, the small size of the elements these sensors can be quite fast. A thermal mass flow sensor measures the asymmetry of temperature profile around the heater which is modulated by the fluid flow. In normal, a mass flow sensor is composed of a central heater and a pair of temperature sensing elements around the heater A new 2-D wide range micro flow sensor structure with three pairs of temperature sensors and a central heater was proposed and numerically simulated by Finite Difference formulation to confirm the feasibility of the flow sensor structure in time domain.
두경부암에서 저용량 Cisplatin 기반 매주 요법의 항암방사선 동시치료의 독성과 예비 임상 결과
김태용(Tae-Yong Kim),김경주(Kyoung Ju Kim),김기환(Ki Hwan Kim),김지은(Ji Eun Kim),박선원(Sun-Won Park),오소원(So Won Oh),정영호(Young Ho Jung) 대한두경부종양학회 2011 대한두경부 종양학회지 Vol.27 No.1
Purpose:Concurrent chemoradiotherapy(CCRT) with 3 weekly cisplatin is the standard treatment of lo-cally advanced head and neck cancer(HNC). The aim is to evaluate the efficacy and toxicities of low-dose week-ly cisplatin-based CCRT, which was devised to reduce the toxicity of CCRT. Method:We retrospectively ana-lyzed HNC patients who received low-dose weekly cisplatin-based CCRT between 2008 and 2010. Cisplatin 35mg/m 2 was weekly given to all patients during radiotherapy. The efficacy was evaluated by the degree of clinical response, treatment failure and survival. The toxicity was evaluated by hematologic toxicities and oral mucositis. Results:A total of 27 patients were analyzed and median age was 59(range 31-81). The ratio of administered dose of radiotherapy and cisplatin to planned dose were 0.98 and 0.93, respectively. Complete re-mission and partial remission were 73% and 23%, respectively. Treatment failure was observed in 8(30%) pa-tients. 1-year survival rate and 1-year disease free survival rate were 82% and 59%, respectively. Overall surviv-al and progression-free survival did not reach median time. Grade 3/4 anemia, neutropenia, thrombocytopenia and oral mucositis were observed in 11%, 19%, 7% and 32% of patients, respectively. In terms of administered cycles, however, only 1-3% of grade 3/4 hematologic toxicities occurred among total 190 cycles. Severe oral mucositis were statistically associated with old age(p=0.003). Treatment failure had no statistical relation with age, pathology, primary site and stage. Conclusion:Low-dose weekly cisplatin-based CCRT seemed to deliv-er enough dose of cisplatin and to show low drop-out rate and good efficacy with low hematologic toxicities.
외상 후 스트레스 장애 환자의 한국판 웩슬러 지능검사 및다면적 인성검사의 특성
이태영(Tae Young Lee),정혜경(Hae Gyung Chung),최진희(Jin Hee Choi),정문용(Moon Yong Chung),최영안(Young An Choi),김태용(Tae Yong Kim) 대한사회정신의학회 2007 사회정신의학 Vol.12 No.2
연구목적: 외상 후 스트레스 장애(PTSD)는 많은 혼란요소로 인해 정확한 평가에 어려움이 있다. 단일 외상에 노출된 PTSD 환자군을 대상으로 임상에서 널리 사용하는 지능검사 및 인성검사를 실시하여 PTSD 증상의 특성을 평가하고자 하였다. 방 법: 11명의 PTSD 환자군과 12명의 정상 대조군을 대상으로 다면적 인성검사, 웩슬러 지능검사를 시행하여 결과를 비교하였다. 결 과: 다면적 인성검사 검사 결과 타당도 척도에서 F 척도가 유의하게 높았고, K 척도가 유의하게 낮았으며, 임상척도에서 Hs, D, Hy, Pd, Pa, Pt, Sc, Si 척도가 유의하게 높았다. 웩슬러 지능검사 검사 결과 환자군에서 언어성 지능이 유의하게 낮았다. 결 론: PTSD군의 MMPI 결과 대부분의 임상척도의 상승 소견을 보였고 지능검사에서 나타난 낮은 언어성 지능은 이전의 연구결과를 지지하는 소견이다. Objectives:It’s difficult to evaluate PTSD due to many comfounding factors. This study was conducted to evaluate the characteristics of PTSD symptoms for patients with PTSD having experienced single traumatic event with K-WIS and MMPI which are widely used clinically. Methods:K-WIS and MMPI were performed and compared on the 11 patients with PTSD and 12 normal comparison. Results:The result of MMPI test concluded that the patients group had significantly high F, low K scale in validity scales, and also significantly high of Hs, D, Hy, Pd, Pa, Pt, Sc, Si in clinical scales. The result of K-WIS test showed that the verbal intelligence was significantly low in patients with PTSD. Conclusion:The MMPI results from the patients with PTSD showed overall elevation in clinical scales. Low verbal intelligence of K-WIS test supports previous study results.
첫 세포검사에서 양성이며 추가 세포검사에서 악성의 증거는 없었으나 경과 관찰 중 현저한 크기 증가로 인하여 수술하게 된 갑상선결절의 수술 후 결과
김의영 ( Eui Young Kim ),김원구 ( Won Gu Kim ),김태용 ( Tae Yong Kim ),공경엽 ( Gyungyup Gong ),류진숙 ( Jin-sook Ryu ),윤종호 ( Jong Ho Yoon ),홍석준 ( Suck Joon Hong ),김원배 ( Won Bae Kim ),송영기 ( Young Kee Shong ) 대한갑상선학회 2009 International Journal of Thyroidology Vol.2 No.2
Background and Objectives: Fine needle aspiration (FNA) cytology is the best test to diagnose malignancy in thyroid nodules. However, cytologic interpretation of FNA specimens is difficult in case that follicular neoplasm is suggested. The aim of this study was to evaluate the rate of malignancy in thyroid nodules which were cytologically diagnosed as benign at the first FNA cytology but the size of nodules increased during the observation period. Materials and Methods: Patients who underwent thyroid surgery in Asan Medical Center from 1996 to 2001 were enrolled. Patients who diagnosed as benign at the first FNA cytology and relatively rapid increase in the nodule size during the observation period were included in this retrospective study. Results: Total 283 patients were included in this study. Their mean age was 42 years and mean tumor size was 4.89 cm (range 2.0∼12.0 cm). In pathologic results, 223 (80.9%) patients had benign thyroid nodules with 191 nodular hyperplasia, 37 follicular adenomas and 1 hashimoto’s thyroiditis, respectively. Fifty four patients (19.1%) had malignancy with 35 follicular carcinomas, 17 papillary thyroid carcinomas and 2 poorly differentiated carcinomas, respectively. Conclusion: Surgical treatment could be recommended for patients with significant increase in size of thyroid nodule, even though the results of their initial FNA were benign.
내비분비-대사 ; Sunitinib이 FRTL-5 세포의 증식 및 생존에 미치는 영향
김원배 ( Won Bae Kim ),김진아 ( Jin A Kim ),김태용 ( Tae Yong Kim ),송영기 ( Young Kee Shong ),한지민 ( Ji Min Han ),최현정 ( Hyun Jeung Choi ),임지혜 ( Ji Hye Yim ),김언구 ( Won Gu Kim ),김읭여 ( Eui Young Kim ) 대한내과학회 2010 대한내과학회지 Vol.79 No.5
목적: 최근 소화기 기질 종양과 신장암의 치료에 사용되고 있는 표적 치료제인 sunitinib은 36~85%의 환자에서 갑상선 기능 이상을 유발하였다. 아직 sunitinib이 갑상선 기능 저하를 일으키는 기전은 명확히 알려진 바가 없다. 본 연구에서는 현재 가장 널리 사용되고 있는 정상 갑상선 세포 모델인 FRTL-5 세포를 이용하여 sunitinib이 갑상선 세포에 미치는 영향을 확인하고자 하였다. 방법: FRTL-5 세포에서 sunitinib이 세포의 증식에 미치는 영향을 갑상선 자극 호르몬(TSH)의 유무에 따라서 확인하였다. 유속 세포 측정 분석을 통해서 세포 주기에 미치는 영향과 세포 자연사의 여부를 확인하였다. 세포 주기의 진행과 관련된 단백 발현량의 변화는 웨스턴 블롯을 통해서 확인하였다. 결과: Sunitinib은 농도와 시간 의존적으로 FRTL-5 세포의 증식을 억제하였으며, TSH가 존재하는 조건에서 그 영향이 뚜렷하였다. FRTL-5의 세포 주기는 sunitinib에 의해서 G1-S기에 멈추었으며, 15 μM의 고농도에서는 caspase-3를 활성화 하고 세포의 자연사를 촉진하였다. TSH에 의해서 기저에서 억제되어 있었던 p21cip1과 p27kip1의 단백 발현이 sunitinib에 의해서 증가되는 것을 웨스턴 블롯으로 확인하였다. 또한, G1-S기 진행에 관련된 cyclin D1, cyclin dependent kinase 2는 sunitinib에 의해서 억제되었다. 결론: 정상 갑상선 세포주인 FRTL-5 세포는 TSH가 존재하는 상태에서 sunitinib에 의해서 세포의 증식을 더욱 억제 하였고, 고농도에서는 세포 자연사를 유발하였다. Sunitinib은 TSH에 의해서 기존에 억제되어 있던 p27kip1/p21cip1 단백발현을 증가시키면서 세포 주기를 G1-S기에 멈추도록 하였으며, 이는 sunitinib이 TSH의 작용을 방해할 수 있음을 보여주고 있다. Background/Aims: Hypothyroidism has been reported in 36~85% of patients treated with sunitinib for renal cell carcinoma or gastrointestinal stromal tumor. However, the mechanism behind this hypothyroidism is unclear. This study evaluated the effects of sunitinib, a multi-target tyrosine kinase inhibitor, on the survival and proliferation of thyrocytes using FRTL-5 rat thyroid cells. Methods: We examined the effect of sunitinib on cell proliferation in the presence and absence of thyroid stimulating hormone (TSH) in a colorimetric assay. Effects on the cell cycle were evaluated by flow cytometry, and on apoptosis using an annexin V apoptosis assay kit and by immunoblotting for caspase-3. Immunoblotting was also used to evaluate changes in the levels of intracellular proteins associated with the G1-S phase of the cell cycle. Results: Sunitinib suppressed the proliferation of FRTL-5 cells in a dose- and time-dependent manner. This suppressive effect was enhanced by the presence of TSH (1 mU/mL). Sunitinib was subsequently shown, in flow cytometric analyses, to arrest the cell cycle at the G1-S phase. Furthermore, it induced apoptosis at a high concentration (15 μM) by activating caspase-3. G1-S phase arrest was associated with the induction of p27kip1 and p21cip1, whose expression is suppressed by TSH under control conditions. Sunitinib also decreased intracellular levels of cyclin D1 and cyclin-dependent kinase 2 in FRTL-5 cells. Conclusions: Sunitinib induced apoptosis in and suppressed the proliferation of FRTL-5 cells. Its suppression of proliferation was further enhanced by the presence of TSH. Sunitinib arrested the cell cycle in the G1-S phase by inducing the expression of p27kip1/p21cip1, which are suppressed by TSH under normal conditions. Collectively, these findings suggest that sunitinib may interfere with TSH signaling pathways in normal thyrocytes. (Korean J Med 79:509-517, 2010)