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Spirulina platensis의 옥외배양 최적화 및 오염생물 구제
김충재,정윤호,최강국,박용하,안치용,오희목,Kim, Choong-Jae,Jung, Yun-Ho,Choi, Gang-Guk,Park, Yong-Ha,Ahn, Chi-Yong,Oh, Hee-Mock 한국조류학회(藻類) 2006 ALGAE Vol.21 No.1
Outdoor cultivation of cyanobacterium Spirulina platensis was carried out for 40 days in a batch mode. A half concentration of the SOT based on the underground water was used as culture medium. Working volume was 5.7 tons with 0.2 m depth. During cultivation, mean water temperature, DO and light intensity were all in proper conditions for the S. platensis growth. The adjustment of pH to over 10 with Na2CO3 and addition of the 1.5% natural salt were very effective to delete contaminant organisms, Chlamydomonas moewusii and Chlorella minutissima occurred one after the other in the culture. The mean productivity of the biomass based on the dry cell weight from 14 to 25 days, after the contaminants were deleted, was 7.8 g ·m–2· d–1, which was relatively high productivity in that a half concentration of the SOT was used for the culture. Underground water used in the culture minimized contaminants invasion and addition of the 1.5% natural salt was effective to delete contaminants as well as acted as mineral supplement in outdoor cultivation of S. platensis. Harvesting using the floating activity of S. platensis was effective from mass floating in day time after overnight without agitation and illumination.
단일 기관에서 필렛피판을 이용한 귀 켈로이드 수술 치료에 대한 연구
김충재,최훈,나찬호,신봉석,김민성 대한이비인후과학회 2020 대한이비인후과학회지 두경부외과학 Vol.63 No.7
Background and Objectives Keloids are benign dermal fibrous growth and excessive collagendeposition that occur usually after trauma or surgery. In the treatment of keloids, the recurrencerate is relatively high after surgical excision. Fillet flap is known to be a good surgicalmethod for keloid lesions. The purpose of this study is to find out manifestation and comparethe results of ear keloids after the surgery by fillet flap. Subjects and Method We retrospectively evaluated 22 patients with ear keloids (n=31) whounderwent core excision with fillet flap at the Department of Dermatology, Chosun UniversityHospital from May 2010 to June 2018. Results With the 22 of treated patients and 31 ear keloid lesions, the average size of keloidlesions was 0.75×1.05 cm2. The frequencies of occurrence with respect to the location of keloidsaccording to the anatomical structure of the ear were 12 lobule (38.7%), 17 helix (54.8%),1 antihelical fold (3.2%), and 1 postauricle (3.2%), respectively. There were 14 lobular types(45.2%), 9 dumbbell types (29.0%), 5 button types (16.1%), and 3 wrap-around type (9.7%). Recurrencewas found in 8 keloid lesions (25.8%) and 5 patients (22.7%) after the surgery. Earlobelesions and dumbbell shaped recurred with the highest recurrence rate. Among the 5 patientswho relapsed, 4 had family history of keloids. Conclusion The earlobe and dumbbell shaped types showed the highest recurrence rate andfamily history was also an important risk factor for recurrence. Also, surgical excision withfillet flap can be very effective and is a good way to treat ear keloids
김충재,김창훈,Yoshihiko Sako 한국수산과학회 2004 Fisheries and Aquatic Sciences Vol.7 No.3
The marine dinoflagellate genus Alexandrium, which produces PSP toxins, has a global distribution. As human-assisted dispersal of the species has been suggested, it is important to develop molecular tools to trace the dispersal pathway. To screen population-specific DNA sequences that differentiate Korean and Japanese A. catenella, we targeted the area downstream of the chloroplast psbA gene using PCR with population-specific DNA primers followed by RFLP (restriction fragment length polymorphism) analysis and sequencing. The RFLP patterns of the PCR products divided Korean and Japanese A. catenella regional isolates into three types: Korean, Japanese, and type CMC3, isolated from Korea. We sequenced the PCR products, but found no similar gene in a homology search. The molecular phylogeny inferred from the sequences separated the Korean and Japanese A. catenella strains, as did the RFLP patterns. However, the Japanese isolates included two slightly different sequences (types J and K), while the Korean sequence was the same as the Japanese K type. In addition, a unique sequence was found in the Korean strains CMC2 and CMC3. Population-specific PCR amplification with Japanese A. catenella type-specific PCR primers designed from the type J sequence yielded PCR products for Japanese strains only, showing that the unknown gene can be used for a population analysis of Korean and Japanese A. catenella.
소아 발모벽에서 N-acetylcysteine 치료효과에 대한 임상적 고찰
김충재 ( Choong Jae Kim ),최훈 ( Hoon Choi ),나찬호 ( Chan Ho Na ),신봉석 ( Bong Seok Shin ),김민성 ( Min Sung Kim ) 대한피부과학회 2019 대한피부과학회지 Vol.57 No.10
Background: Trichotillomania (TTM) is a medical condition that involves recurrent and irresistible urges to pull out their own hair. TTM generally follows a chronic course in most patients and is difficult to treat and manage. Recently, N-acetylcysteine (NAC) has shown efficacy in the treatment of TTM. Objective: The purpose of this study was to assess the efficacy of treatment with NAC in trichotillomania children younger than 13 years of age. Methods: We evaluated 15 patients under 13 years old who visited the Department of Dermatology at Chosun University Hospital between 2014 and 2019 for TTM. An initial dose of 400-mg NAC was administered to patients under 8 years and 800 mg to patients older than 8 years old. If there was no improvement for 2 weeks, the dose was increased with the maximum daily being 2,400 mg. Results: The mean age of patients was 10.7 years; 8 were boys and 7 were girls. They presented with several asymptomatic focal patches of hair loss with different hair lengths on scalp. The dermoscopic findings were broken hairs, v-sign hairs, coiled hairs, and focal erythema. 10 out of 15 patients had a good response to NAC, whereas 5 patients did not respond to the treatment. The differences between the 2 groups were the treatment duration and total NAC doses. The effective group had a mean NAC of 973 mg/day for 11.5 weeks, while the non-effective group had a 1060 mg/day dose for 4 weeks. Conclusion: NAC could be an effective and safe treatment option for TTM in children. (Korean J Dermatol 2019;57(10):600∼607)
옵티컬 일루젼의 효과적인 접목을 위한 제품 디자인 사례연구
김충재 ( Chunjae Kim ),김보연 ( Bo Yeun Kim ) 커뮤니케이션디자인학회 2015 커뮤니케이션 디자인학연구 Vol.53 No.-
제품이 사용자에게 도달하기까지 수많은 생산과정과 제약을 거치면서 대칭적이고 정형화된 형태의 디자인을 갖게 된다. 하지만 현대 소비자들은 보다 빠르게 새롭고 독특한 제품을 원하고 디자이너들은 그에 따라보다 자극적이며 실험적인 형태의 제품들을 끊임없이 연구한다. 이러한 흐름에 부합하고자 본 연구는 메종&오브제 파리 2015가 선정한 올해의 디자이너인 ``오키 사토``와 그의 디자인 스튜디오 ``넨도``가 중점적으로 활용한 옵티컬 일루젼에 주목하여 옵티컬의 개념과 배경을 검토한다. 또한 그와 관련 사례들을 Wucius Wong의 체험 조형요소에 따라 면밀히 분석함으로서 앞으로의 옵티컬 일루젼이 접목된 제품디자인 방향을 논한다. Generally a product has symmetric and valanced design by being gone through many processes and regulations in its production. However, contemporary designers study more experimental design for their consumers who are eagerly looking for more unique design. In order to follow the stream of product design, this study focus on optical illusion which is recently used by the winner of Meson & Object paris 2015, ``Oki Sato``, design studio ``Nendo``. Moreover, this study explores and describes contemporary product design with optical illusion. Specifically, every product in this study was analysed by the elements of design by Wucius Wong. In conclusion, it results and suggests the future of product design.
10년간 단일 기관에서의 귀 켈로이드 임상 및 역학 연구
김충재 ( Choong Jae Kim ),최훈 ( Hoon Choi ),나찬호 ( Chan Ho Na ),신봉석 ( Bong Seok Shin ),김민성 ( Min Sung Kim ) 대한피부과학회 2021 대한피부과학회지 Vol.59 No.1
Background: Keloids are benign dermal fibrous growths associated with excessive collagen deposition that usually occurs after trauma or surgery. To date, the clinical features and epidemiology of ear keloids have not been comprehensively investigated. Objective: We investigated the epidemiology and clinical characteristics of ear keloids in Koreans. Methods: We retrospectively investigated Koreans diagnosed with ear keloids by dermatologists at the Department of Dermatology, Chosun University Hospital, between January 2010 and December 2019. Results: The study included 95 patients with 120 ear keloid lesions (mean size of lesions 1.12×1.45 ㎠). Based on the ear anatomy, the keloids were observed at the following sites: the helix in 61 (50.8%) patients, the earlobe in 37 (30.8%) patients, antihelix in 10 (8.3%) patients, scapha in 3 (2.5%) patients, and the postauricular region in 9 (7.5%) patients. Among the keloids evaluated, 67 (55.8%), 36 (30.0%), 9 (7.5%), and 8 (6.7%) were categorized as lobular, dumb-bell, button, and wrap-around types, respectively. Most (75%) keloids were located on the posterior surface of the ears. Notably, 26 patients had a family history of keloids, which was significantly correlated with the development of keloids at other sites (p<0.001). Conclusion: The helix was the most common site of involvement, and lobular keloids represented the most common variety of keloids observed in this study. Keloids occurred most frequently on the posterior surface of the ears and were bigger at these sites. Patients with a family history of keloids were significantly more likely to develop keloids in areas other than the ears. (Korean J Dermatol 2021;59(1):8∼14)