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인간 배아 동결 해빙시 액체질소의 분사속도가 배아 발달 및 임신에 미치는 영향
김영아,서성석,김미란,황경주,박동욱,조미영,유희석,Kim, Young-Ah,Seo, Seong-Seog,Kim, Mi-Ran,Hwang, Kyung-Joo,Park, Dong-Wook,Jo, Mi-Yeong,Ryu, Hee-Suk 대한생식의학회 2001 Clinical and Experimental Reproductive Medicine Vol.28 No.4
Objective : To investigate the efficacy of high infusion frequency of liquid nitrogen on pregnancy in human embryo after freezing and thawing. Materials and Methods: 150 infertile patients underwent 162 consecutive thawing-ET cycles. In the high infusion frequency group (Group A), 47 patients (50 cycles) underwent cryopreservation with high infusion frequency of liquid nitrogen. In the low infusion frequency group (Group B), 103 patients (112 cycles) underwent cryopreservation with low infusion frequency of liquid nitrogen. We analyzed the clinical characteristics, fertilization rates, development of embryo, good quality embryo ratio, implantation rates, and pregnancy rates between these two groups. Results: There was no difference between the groups with regard to clinical characteristics (mean age, infertility duration, infertility factors, hormone profile), mean number of oocyte retrieval, fertilization rates, and mean embryo number of transfers. The survival rates in group A was 64.9% (228 of 350 embryos), and among the 228 embryos 190 embryos (83.3%) which progressed to the two- to eight-cell stage. After thawing, the embryo numbers were 65 (34.2%), 29 (15.3%), 35 (18.4%), and 37 (19.5%) of grades 1, 2, 3, and above 4, respectively. The survival rates in group B was 63.8% (482 of 755 embryos), and among the 482 embryos 465 embryos (96.5%) which progressed to the two- to eight-cell stage. After thawing, the embryo numbers were 106 (22.8%), 94 (20.2%), 89 (19.1%), and 112 (24.1%) of grades 1, 2, 3, and above 4, respectively. There was no difference in embryo quality change after the freezing-thawing procedure between the groups. Implantation rates (31.1% vs. 34.3%) were not significant. However hCG positive rates in group A (40%) were higher than group B, but not statistically significant. Clinical pregnancy rate (26% vs. 25.9%), on going pregnancy rates (>20 weeks) were not significant (26% vs. 25%). Conclusion: We compared embryo quality change, survival rates, and pregnancy rates between high infusion frequency group and low infusion frequency group and the results were similar between the two groups. Therefore, high infusion frequency of liquid nitrogen for cryopreservation is a worthy method to preserve in human embryos.
응급실 간호사를 위한 공감피로 극복프로그램 개발 및 효과검증
김영아(Kim, Yeong Ah),박정숙(Park, Jeong Sook) 한국간호과학회 2016 Journal of Korean Academy of Nursing Vol.46 No.2
Purpose: This study was conducted to develop a program to help emergency nurses overcome compassion fatigue, and to analyze the effects of the program. Methods: A nonequivalent control group pretest-posttest design was used. There were 14 participants in the experimental group and 18 subjects in the control group. The program was comprised of five, weekly 80-minute sessions including understanding and assessment of compassion fatigue, enhancing positive affect, balancing work-life, planning self care, training in relaxation techniques and cognitive restructuring, and getting social support. Research variables were ego-resiliency, compassion satisfaction and compassion fatigue of the ProQOL 5, and salivary cortisol. Data were analyzed using Chi-square test, independent t-test, and paired t-test. Results: The first hypothesis, “There will be a difference in scores for ego resiliency between the experimental group and the control group”. was not supported. The second hypothesis, “There will be a difference in scores for compassion satisfaction between the experimental group and the control group” was supported (t=2.15, p =.046). The third hypothesis, “There will be a difference in scores for compassion fatigue between the experimental group and the control group” was not supported. Conclusion: The first program for emergency nurses to overcome compassion fatigue in Korea was effective in increasing emergency nurses’ compassion satisfaction and decreasing salivary cortisol level in the experimental group. Therefore, this program for overcoming compassion fatigue is useful to increase emergency nurses’ compassion satisfaction. However replication studies of short-term intensive program reflecting emergency nurses’ opinion are needed.
김영아 ( Young Ah Kim ),안상태 ( Sang Tae Ahn ),조미영 ( Mi Yeong Jo ),유승철 ( Seung Chul Yoo ),김미란 ( Mi Ran Kim ),황경주 ( Kyung Joo Hwang ),유희석 ( Hee Sug Ryu ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.4
목적 : 복강경수술을 시행받은 환자들을 대상으로 복강경수술의 장점, 단점, 적용범위 및 합병증 등에 대해 알아보고자 하였다. 연구 방법 : 1994년 6월 1일부터 2001년 8월 31일까지 아주대학교병원 산부인과에서 복강경수술을 시행 받은 1711명의 환자를 대상으로 후향적 고찰을 시행하였다. 결과 : 환자군의 나이 분포는 11세에서 77세까지로 평균 31.5세였다. 복강경수술의 적응증은 자궁외 임신이 626예 (36.6%), 자궁 내막증, 난소 종양 Objective : To assess the advantages, disadvantages, applicability, and complications of laparoscopy in gynecologic diseases. Materials and Methods : A retrospective case series which includes 1711 patients who underwent laparoscopic surgery at Department
HCG 에 의하여 억제된 ATP 유도 인간 난소 황체화 과립막세포의 세포자멸사에 관한 연구
김영아(Young Ah Kim),김미란(Mi Ran Kim),황경주(Kyung Joo Hwang),박동욱(Dong Wook Park),조태섭(Tae Seop Cho),민철기(Churl K Min),조미영(Mi Yeong Jo) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.3
N/A Objective: To determine the effects of hCG on extracellular ATP induced apoptosis in cultured human luteinized granulosa cells (hLGCs) Methods: The addition of various concentrations of ATP (0,0.1,0.25,0.5,0.75mM)and5IuhCG to luteinized granulosa cells obtained during in vitro fertilization ovum pickup procedures. After culture for 24 hours, purinoceptor activity and functional changes in mitochondria were measured by patch clamp, flow cytometry, and confocal microscopy. Results: Calcium imaging with fura-2 revealed that ATP elevated [Ca 2+]i by mobilizing intracellularly stored Ca 2+. A patch clamp study showed that ATP exerted its effect by initially binding to the P2Y type purinoceptor, as evidenced by the ATP-evoked outward Ca 2+ -activated K +current. Probing mitochondria with JC-1, a mitochondrial transmembrane potential-sensitive dye, revealed that ATP induced mitochondrial depolarization in a concentration-dependent manner. A quantitative flow cytometric analysis with Annexin V showed that apoptotic cells were increased in number in proportion to the concentration of ATP, having 18.57% of apoptotic cell populations in the presence of 0.75mM ATP compared to 7.88%in the control. Moreover, treatments with human chorionic gonadotropin (hCG) at 5 IU reversed both the ATP-induced mitochondrial depolarization and apoptosis (18.57 vs 6.32%). Conclusion: Taken together, these results indicate, first, extracellular ATP recognized by the P2Y type purinoceptor on h-LGCs increases the intracellular Ca2+ . Second, the increased intracellular Ca2+ triggers the apoptotic cascade by acting at least, in part, on mitochondria. Third, hCG reverses the ATP-induced apoptosis, raising a possible clinical implication of hCG in the treatment of degeneration of granulosa cells such as follicular atresia.
50-g 경구 당부하 검사의 결과와 임신성 당뇨와의 상관관계에 관한 연구
김종화 ( Kim Jong Hwa ),서용수 ( Seo Yong Su ),최성진 ( Choe Seong Jin ),김영아 ( Kim Yeong A ),노정래 ( No Jeong Lae ),양순하 ( Yang Sun Ha ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.11
목적 : 50-g 경구당부하 검사결과와 임신성 당뇨의 상관관계와 임신성 당뇨의 진단이 가능한 50g 경구 당부하검사의 결과치를 결정할 수 있는지 여부 및 50-g 경구 당부하검사결과가 200 mg/dl 이상을 기준으로 임신성 당뇨의 진단이 가능한지를 알아보고자 한다. 연구 방법 : 1998년 7월부터 2002년 5월까지 50 g 경구당부하검사를 실시한 임신부 중 결과치가 140 gm/ dl 이상인 1353명을 대상으로 100 g 경구당부하검사를 실시하여 Objective : To evaluate the relationship between results of 50-g one-hour glucose screening test (50 GTT) and gestational diabetes and to find cut-off result value of 50 g GTT which can diagnosis the gestational diabetes without other confirmative tests.
만삭 임신 수태 조직에서 유도체에 따른 Nuclear Factor Kappa B의 활성화 양상
김종화 ( Kim Jong Hwa ),김영아 ( Kim Yeong A ),양순하 ( Yang Sun Ha ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.9
목적 : Nuclear factor kappa B (NF-B)는 prostaglandin 생성에 중요한 효소인 cyclooxygenase 2 (COX-2)의 전사 인자이다. 본 연구에서는 NF-B의 활성을 유도하는 여러 유도체 (inducer) 처치시 자궁근, 융모막, 양막 세포에서 NF-B의 활성화 양상을 비교하였다. 연구 방법 : 진통이 없던 만삭의 임부의 제왕절개시 자궁근, 융모막, 양막 조직을 수집하여 세포를 분리하여, IL-1β, TNF-α 그 Objective : Nuclear factor kappa B (NF-B) is a transcriptional factor in the expression of cyclooxygenase 2 (COX-2), the key enzyme in production of prostaglandins. The purpose of this study was to investigate the activation of NF- B in human gestational