Arbutamine부하 심초음파도의 안정성과 유용성

신이철(Yi Chul Synn),김기식(Kee Sik Kim),배장호(Jang Ho Bae),한성욱(Seong Wook Han),박소영(So Young Park),남창욱(Chang Wook Nam),김기영(Ki Young Kim),김윤년(Yoon Nyun Kim),김권배(Kwon Bae Kim),김여희(You Hee Kim) 대한내과학회 2000 대한내과학회지 Vol.58 No.1

N/A Background : Exercise and pharmacologic stress echocardiography are widely used for detecting coronary artery disease. Arbutamine is a new synthetic mild α1-receptor and - receptor agonist developed specifically for stress echocardiography. Arbutamine is superior to dobutamine owing to its enforced chronotropic action than that of dobutamine. We intended to know safety and efficacy of arbutamine stress echocardiography in inducing myocardial ischemia and detecting coronary artery disease. Methods : We underwent arbutamine stress echocardiography on 52 patients, dobutamine stress echocardiography in 35 patients. Alteration of blood pressure, heart rate, regional wall motion on echocardiography were evaluated. Sensitivity and specificity were determined by coronary angiography for 61 patients(Arbutamine: 31, Dobutamine : 30) Results : 1) Hemodynamic alterations respect to stress agents Baseline Maximal Baseline Maximal Interval for Blood pressure Blood pressure Heartrate Heart rate maximal heartrate Arbutamine 122/70mmHg 138/72mmHg 69BPM 137BPM 8.2 min* Dobutamine 126/73mmHg 136/77mmHg 74BPM 102BPM 11.4 min* (* p < 0.05) 2) Comparison of Arbutamine and Dobutamine in sensitivity Sensitivity(Specificity) Side effects Atropine Arbutamine 80.1% (90%) 33(63.5%) 8(15.4%) Dobutamine 78.2% (71.4%) 21(60%) 7(20%) 3) Side effects of stress agents Hypotension Palpitation, tremor Arrhythmia Chest pain Arbutamine 15(28.8%)* 4(7.7%)* 21(40.4%) 8(9.2%) Dobutamine 3(8.6%)* 9(25.7%)* 12(34.3%) 5(5.7%) (* p < 0.05) 4) Premature ventricular contraction was most common arrhythmia in both group. There was no fatal or significant complication, and most complications were subsided after discontinuation of stress agents. Conclusion : Arbutamine is an effective and safe pharmacologic stress agent in detecting myocardial ischemia and superior to dobutamine in increasing heart rate. Sensitivity and specificity of arbutamine were higher than that of dobutamine.(Korean J Med 58:39-47, 2000)

Ethanol 중독 흰쥐에서 총담관결찰이 간의 Glutathione S-Transferase, Glutathione Peroxidase 및 Glutathione Reductase 활성에 미치는 영향

곽춘식,김여희,조준승,Kwak, Chun-Sik,Kim, You-Hee,Jo, Joon-Seung 생화학분자생물학회 1990 한국생화학회지 Vol.23 No.2

급성 및 만성 주정중독 흰쥐에서 담즙울체가 간의 glutathione S-transferase(GST), glutathione reductase(GR) 및 glutathione peroxidase(GSH-Px) 활성 변동에 어떤 영향을 미치는가를 알아보기 위하여 이 실험을 하였다. 흰쥐에게 만성 주정중독을 시켰을 때 간의 세포질과 microsome 분획의 GST 활성도는 약간 증가 되었다. 그러나 mitochondria 분획의 GST 활성도는 변동을 나타내지 않았다. 또한 만성 주정 중독군에서 간의 GR 및 간의 세포질 분획의 GSH-Px의 활성도와 급성 주정중독군에서 간 세포질, mitochondria 및 microsome 분획의 GST, 간의 GR과 간의 세포질 분획의 GSH-Px 활성도 등도 변동이 없었다. 만성 주정중독 후 총담관을 결찰한 군에서 간 세포질 분획의 GST 활성도는 실험의 전기간을 통해 의의 있는 활성 감소를 나타내었다. 그러나 감소의 정도는 총담관결찰군보다 현저하지 않았다. 그리고 간세포 mitochondria 분획에서 GST 활성도는 총담관결찰군이 총담관결찰 후 14일에 약간 감소되었으나 만성 주정중독 후 총담관을 결찰한 군에서는 2일 부터 14일까지 의의 있는 감소를 나타내었다. 또한 microsome 분획의 GST 활성도는 총담관결찰군에서는 총담관결찰 후 7일 및 14일에 현저한 증가를 나타내었다. 그러나 만성 주정증독 후 총담관을 결찰한 군에서는 실험의 전기간을 통해 증가를 보이지 않았다. 만성 주정중독 후 총담관을 결찰한 군에서 간의 GR 활성도는 총담관결찰 후 2일부터 14일까지 증가를 나타내었으나 그 증가의 정도는 총담관결찰군보다 현저하지 않았다. 반면에, 만성 주정중독 후 총담관을 결찰한 군에서 GSH-Px 활성도는 총담관결찰 후 14일에 의의 있는 감소를 나타내었다. 그러나 그 감소의 정도는 총담관결찰군보다 현저하지 않았다. 총담관결찰 14일 후 급성 주정증독을 시킨 군에서 간 세포질 분획의 GST와 GSH-Px의 활성도는 현저한 감소를 나타내었다. 그러나 그 활성 감소의 정도는 총담관결찰만 하고 14일에 희생시킨 군보다는 현저하지 않았다. 총담관결찰 14일 후 급성 주정중독을 시킨 군에서 간세포 microsome 분획의 GST와 간의 GR 활성도는 현저한 증가를 나타내었다. 그러나 그 활성 증가의 정도는 총담관결찰만 한 후 14일에 희생시킨 군보다 현저하지 않았다. 총담관결찰 14일 후 급성 주정중독을 시킨 군에서 간세포 mitochondria 분획의 GST 활성도는 총담관결찰만 한 후 14일에 희생시킨 군과 마찬가지로 의의 있는 활성 감소를 나타내었다. 이상 실험결과를 보아 급성 및 만성 주정중독시 간에 담즙울체가 야기되면 endoplasmic retieulum의 GST와 세포질의 GR 활성도가 간에 담즙울체만 야기시켰을때 보다 그 활성이 감소되는 것으로 보이며 반면에 간의 세포질 분획의 GST와 GSH-Px 활성도는 증가되는 것 같았다. 그리고 만성 주정중독시에만 세포질과 endoplasmic reticulum의 GST 활성도가 약간 증가되는 것 같았다. This study was made to see the effect of common bile duct ligation on liver glutathione S-transferase(GST), glutathione peroxidase(GSH-Px) and glutathione reductase(GR) activities in rats suffering from acute and chronic intoxication of ethanol. For chronic intoxication of ethanol, the rats were fed 5%(v/v) ethanol instead of water for 60 days. Common bile duct of the same group of rats were ligated with ethanol constantly being fed. The rats were then killed on the 1st, 2nd, 3rd, 7th and 14th days of the procedure to measure the cytosolic, mitochondrial and microsomal GST, and cytosolic GSH-Px activities of the liver. The liver GR activities were also measured. For acute intoxication of ethanol, 4g of ethanol were administered orally per kg of body weight as a single dose. The rats were killed at the 1.5th and 24th hours of the procedure for study. On the 14th day following common bile duct ligation, the rats were acutely intoxicated with ethanol to be killed at the 1.5th and 24th hours for measuring the activities of the above enzyme. The rats liver cytosolic and microsomal GST activities showed slight increase in chronically ethanol intoxicated group but the mitochondrial GST activities did not increase in this group. The rats liver cytosolic, mitochondrial and microsomal GST activities showed no significant changes in acutely ethanol intoxicated groups. In terms of rats liver GR and liver cytosolic GSH-Px activities, no significant changes were shown in either chronically ethanol intoxicated groups or acutely ethanol intoxicated groups. The groups that received common bile duct (CBD) ligation after being chronically intoxicated with ethanol showed considerable decrease in the liver cytosolic GST activities. However, the activities showed a less degree than groups of CBD ligation. The liver mitochondrial GST activities of the CBD ligation groups showed slight decrease at the 14th day of the ligation. But the activities of the groups with the ligation after chronic ethanol intoxication showed of the ligation. But the activities of the groups with the ligation after chronic ethanol intoxication showed significant decrease at the 2nd, 3rd, 7th and 14th days following the operation. The liver microsomal GST activities of the (CBD) ligation groups showed remarkable increase at the 7th and 14th days of the ligation. But the activities showed no significant increase in the groups with the ligation following the chronic ethanol intoxication. The groups that received CBD ligation after being chronically intoxicated with ethanol showed considerable increase at the 2nd, 3rd, 7th and 14th days following the operation in the liver GR activities. On the other hand, the liver cytosolic GSH-Px activities showed significant increase at the 14th days after the ligation. However, the activities showed a far less on the same time points than the groups only with the CBD ligation. At the 1.5th and 24th hours following the acute intoxication with ethanol which was done after 14 days of the CBD ligation, the rats showed less remarkable decrease in the liver cytosolic GST and GSH-Px activities than the group only with the 14th day following CBD ligation. The liver microsomal GST liver GR activities, however, showed considerable increase at the 1.5th and the 24th hours following the acute intoxication with ethanol which was done after 14 days of the CBD ligation. But the activities showed a less degree than group with the 14th day following CBD ligation. At the 1.5th and the 24th hours following the acute intoxication with ethanol which was done after 14 days of the CBD ligation, the liver mitochondrial GST activity decreased significantly, and the same was seen in the group with the 14th day following CBD ligation.

Ethanol 중독 흰쥐에서 총담관결찰이 간의 Glutathione S - Transferase , Glutathione Peroxidase 및 Glutathione Reductase 활성에 미치는 영향

곽춘식,김여희,조준승 ( Chun Sik Kwak,You Hee Kim,Joon Seung Jo ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.2

This study was made to see the effect of common bile duct ligation on liver glutathione S-transferase(GST), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) activities in rats suffering from acute and chronic intoxication of ethanol. For chronic intoxication of ethanol, the rats were fed 5% (v/v) ethanol instead of water for 60 days. Common bile duct of the same group of rats were ligated with ethanol constantly being fed. The rats were then killed on the 1st, 2nd, 3rd, 7th and 14th days of the procedure to measure the cytosolic, mitochondrial and microsomal GST, and cytosolic GSH-Px activities of the liver. The liver GR activities were also measured. For acute intoxication of ethanol, 4g of ethanol were administered orally per kg of body weight as a single dose. The rats were killed at the 1.5th and 24th hours of the procedure for study. On the 14th day following common bile duct ligation, the rats were acutely intoxicated with ethanol to be killed at the 1.5th and 24th hours for measuring the activities of the above enzyme. The rats liver cytosolic and microsomal GST activities showed slight increase in chronically ethanol intoxicated group but the mitochondrial GST activities did not increase in this group. The rats liver cytosolic, mitochondrial and microsomal GST activities showed no significant changes in acutely ethanol intoxicated groups. In terms of rats liver GR and liver cytosolic GSH-Px activities, no significant changes were shown in either chronically ethanol intoxicated groups or acutely ethanol intoxicated groups. The groups that received common bile duct (CBD) ligation after being chronically intoxicated with ethanol showed considerable decrease in the liver cytosolic GST activities. However, the activities showed a less degree than groups of CBD ligation. The liver mitochondrial GST activities of the CBD ligation groups showed slight decrease at the 14th day of the ligation after chronic ethanol intoxication showed significant decrease at the 2nd, 3rd, 7th and 14th days following the operation. The liver microsomal GST activities of the CBD ligation groups showed remarkable increase at the 7th and 14th days of the ligation. But the activities showed no significant increase in the groups with the ligation following the chronic ethanol intoxication. The groups that received CBD ligation after being chronically intoxicated with ethanol showed considerable increase at the 2nd, 3rd, 7th and 14th days following the operation in the liver GR activities. On the other hand, the liver cytosolic GSH-Px activities showed significant increase at the 14th days after the ligation. However, the activities showed a far less degree on the same time points than the groups only with the CBD ligation. At the 1.5th and 24th hours following the acute intoxication with ethanol which was done after 14 days of the CBD ligation, the rats showed less remarkably decrease in the liver cytosolic GST and GSH-Px activities than the group only with the 14th day following CBD ligation. The liver microsomal GST and liver GR activities, however, showed considerable increase at the 1.5th and the 24th hours following the acute intoxication with ethanol which was done after 14 days of the CBD ligation. But the activities showed a less degree than group with the 14th day following CBD ligation. At the 1.5th and 24th following the acute intoxication with ethanol which was done after 14 days of the CBD ligation, the liver mitochondrial GST activity decreased significantly, and the same was seen in the group with the 14th day following CBD ligation.

저산소 조건에서 항암제와 니트로이미다졸 복합제제가 인간 간암 세포의 생존에 미치는 영향

임선하(Sun Ha Lim),이준엽(June Yeob Lee),박성환(Sung Hwan Park),김여희(You Hee Kim),서헌석(Hun Suk Suh),박재복(Jae Bok Park),이종원(Jongwon Lee) 대한외과학회 2009 Annals of Surgical Treatment and Research(ASRT) Vol.76 No.6

Purpose: In a previous study, we have shown that anticancer agents inhibiting topoisomerases improve survival of tumor cells under hypoxic condition. In the present study, we evaluated whether and how cell survival effect of the anticancer agents under hypoxic conditions could be eliminated by the addition of nitroimidazoles, a class of bioreductive agents. Methods: Human hepatocellular carcinoma cells (HepG2) were incubated with different combinations of pimonidazole(1∼1,000μg/ml) and doxorubicin (0.1 or 1μg/ml) concentrations under different O₂ concentrations [1, 3, 5, 10 and 21 O₂]. Then cell numbers, glucose concentrations and lactic acid concentrations in the medium were measured, and DNA fragmentation assay was performed. Finally, different combinations of nitroimidazoles, such as pimonidazole, misonidazole, etanidazole, tinidazole, metronidazole, ornidazole or dimetridazole, and anticancer agents, such as doxorubicin, campothecin, epirubicin, dactinomycin, etoposide or mitomycin C was added to the cell culture medium under hypoxic conditions (1% O₂). Results: Pimonidazole at a concentration of 100μg/ml eliminated cell survival effect of doxorubicin at the concentrations of 0.1 and 1μg/ml under hypoxic condition (1% O₂) by promoting apoptosis. Almost all the cells died even after 24 hours of incubation for all the oxygen concentrations at a combination of 100μg/ml pimonidazole and 1μg/ml doxorubicin. Finally, pimonidazole at a concentration of 100μg/ml, and misonidazole or etanidazole at a concentration of 1,000μg/ml eliminated cell survival effect of all the anticancer agents tested under hypoxic condition. Conclusion: Combination therapy of doxorubicin (adriamycin) with pimonidazole can maximize dororubicin efficacy by eliminating cell survival effect of doxorubicin under hypoxic conditions in treating solid tumors, such as breast cancer.

흰쥐 재생간에서 활성 산소 대사

박세관(Sea Kwan Park),박학열(Hak Youle Park),문교철(Kyo Cheol Mun),김여희(You Hee Kim),곽춘식(Chun Sik Kwak) 대한소화기학회 2002 대한소화기학회지 Vol.40 No.4

Background/Aims: To know the metabolism of oxygen free radicals in the regenerating liver, the 70% (median and left lateral lobes) partial hepatectomy was performed in the Sprague-Dawley rats. Methods: The original livers were obtained immediately after the partial hepatectomy, while the regenerating livers were obtained at 3 days after the partial hepatectomy. The levels of xanthine oxidase, superoxide anion, antioxidant enzymes, hydrogen peroxide, and malondialdehyde were measured. RT-PCR for superoxide dismutase was also performed. Results: Compared to the original livers, the regenerating liver showed higher levels of xanthine oxidase, superoxide dismutase, catalase, glutathione peroxidase superoxide production, and malondialdehyde and lower level of hydrogen peroxide. RT- PCR did not show any significant change. Conclusions: The results suggest that malondialdehyde level is increased by xanthine oxidase via the increased production of superoxide anion. Superoxide dismutase activity is induced by the increase of superoxide anion. The increase of catalase and glutathione peroxidase levels causes the depletion of hydrogen peroxide level in the regenerating liver. (Korean J Gastroenterol 2002; 40: 255-262)

무중력의 모형으로 Head-down Suspension을 시행한 흰쥐에서 항산화 효소들의 활성도 변동

배재훈(Jae-Hoon Bae),박원균(Won-Kyun Park),송대규(Dae-Kyu Song),채의업(E Up Chae),곽춘식(Chun-Sik Kwak),김여희(You-Hee Kim),문교철(Kyo-Cheol Mun) 한국항공우주의학협회 1997 항공우주의학회지 Vol.7 No.4

급성 주정 중독 흰쥐에서 간의 Aryl sulfotransferase and UDP-Glucuronosyltransferase 활성에 미치는 총담관 결찰의 영향

권삼옥,김여희 啓明大學校 醫科大學 1997 계명의대학술지 Vol.16 No.2

The activities of aryl sulfotransferase (AST) isozymes Ⅰ-Ⅱand Ⅲ-Ⅳand UDP-glucuronosyltransferase (UDP-GT) were measured in rats with acute ethanol intoxication after common bile duct(CBD) ligation to investigate the adverse effects of alcohol ingestion to the liver under the hepatobiliary disease. Serum AST Ⅰ-Ⅱ and AST Ⅲ-Ⅳ activities in CBD ligated rats combined with acute ethanol intoxication showed greater increases than that in CBD ligation alone. These results indicate that the leak of hepatic AST isozymes into blood is facilitated in CBD ligated rats combined with acute ethanol intoxication than in CBD ligation alone due to aggravated hepatic membrane damage. Liver mitochondrial AST Ⅰ-Ⅱ and AST Ⅲ-Ⅳ activities in CBD ligated rats combined with acute ethanol intoxication showed less increases than that in CBD ligation alone. However, liver microsomal UDP-GT activity in CBD ligated rats combined with acute ethanol intoxication showed a more decrease than that in CBD ligation alone. On the other hand, when acute ethanol intoxication was combined with CBD ligation, the values of Vmax of the liver mitochondrial AST Ⅰ-Ⅱ, AST Ⅲ-Ⅳ and liver microsomal UDP-GT decreased significantly than that in CBD ligation alone. However, the values of Km of above hepatic enzymes did not change. Viewed from these results, when acute ethanol intoxication was combined with cholestasis, these enzymes in the liver seem to be decreases their biosynthesis than that in cholestasis. According to all of the results, if ethanol intoxication is combined with cholestasis, the hepatic damage is aggravated.

흰쥐 재생간의 Leucine Aminopeptidase의 활성치

문교철,곽춘식,김여희 啓明大學校 醫科大學 1986 계명의대학술지 Vol.5 No.2

Changes in the activities of the followings have been studied over a period of 6 days after partial hepatectomy in rats: Plasma membrane, mitochondrial, nuclear and cytosolic leucine aminopeptidase and microsomal-particle bound aminopeptidase of the regenerating liver and serum leucine aminopeptidase. The activities of alkaliine phosphatase in the subcellular fractions were also measured. The activities of leucine aminopeptidase and alkaline phosphatase in serum markedly elevated from 12 hours to three days after partial hepatectomy in rats. The activity of plasma memberane-bound leucine aminopeptidase in the regenerating rat liver drastically increased during the first and the third days of the operation. And the activity of microsomal particle-bound aminopeptidase in the regenerating liver significantly increased between the second and the third days after operation. The activities of nuclear and cytosolic leucine aminopeptidase in the regenerating liver showed a substantial increase at the second day and from first to sixth day respectively after operation. However, no significant change in hepatic mitochondrial leucine aminopeptidase was noted throughout the experiments. The activities of plasma membrane, microsomal, mitochondrial, nuclear and cytosolic alkaline phosphatase in the regenerating liver markedly increased throughout the experiments.

Ethanol중독 흰쥐에서 총담관결찰이 간의 Glutathione S-Transferase, Glutathione Peroxidase 및 Glutathione Reductase 활성에 미치는 영향

곽춘식,박은미,문교철,김여희 啓明大學校 醫科大學 1989 계명의대학술지 Vol.8 No.2

This study was made to see the effect of common bile duct ligation on liver glutathione S-transferase (GST), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) activities in rats suffereing from acute and chronic intoxication of ethanol. For chronic intoxication of ethanol, the rats were fed 5%(v/v) ethanol instead of water for 60 days. Common bile duct of the same group of rats were ligated with ethanol constantly being fed. The rats were then killed on the lst, 2nd, 3rd, 7th and 14th days of the procedure to measure the cytosolic, mitochondrial and microsomal GST, and cytosolic GSH-Px activities of the liver, The liver GR activities were also measured. For acute iintoxication of ethanol, 4g of ethanol were administered orally per kg of body weight as a single dose. The rats were killed at the 1.5th and 24th hours of the procedure for study. On the 14th day following common bile duct ligation, the rats were acutely intoxicated with ethanol to be killed at the 1.5th and 24th hours for mesuring the activities of the above enzyme. The rats liver cytosolic and microsomal GST activities showed slight increase in chromically ethanol intoxicated group but the mitochondrial GST activities did not increase in thies group. The rats liver cytosolic, mitochondrial and microsomal GST activities showed no significatn changes in acutely ethanol intoxicated groups. In terms of rats liver GR and liver cytosolic GSH-Px activities, no significant changes were shown in either chronically ethanol intoxicated groups or actely ethanol intoxicated groups. The groups that received common bile duct (CBD) ligation after being chronically intoxicated with ethanol showed considerable decrease in the liver cytosolic GST activities. However, the activities showed a less degree than groups of CBD ligation. The liver mitochondrial GST activities of the CBD ligation groups showed slight decrease at the 14th day of the ligatioin. But the activities of the groups with the ligation after chronic ethanol intoxication showed of the ligation. But the activities of the groups with the ligation after chronic ethanol intoxicatioin showed significant decrease at the 2nd, 3rd, 7th and 14th days followiing the operation. The liver microsomal GST activities of the (CBD) ligation groups showed remarkable increase at the 7th and 14th days of the ligation. But the activities showed no significant increase in the groups with the ligation following the chronic ethanol intoxication. The groups that received CBD ligation after being chronically intoxicated with ethanol showed considerale inicrease at the 2nd, 3rd, 7th and 14th days following the operation in the liver GR activiites. On the other hand, the liver cytosolic GSH-Px activities showed significant increase at the 14th days after the ligation. However, the activities showed a far less on the same time points than the groups only with the CBD ligation. At the 1.5th and 24th hours followiing the acute intoxication with ethanol which was done after 14 days of the CBD ligation, the rats showed less remarkable decrease in the liver cytosolic GST and GSH-Px activities than the group only with the 14th day following CBD ligatioin. The liver microsomal GST liver GR activites, howvere, showed considerable increase at the 1.5th and the 24th hours following the acute intoxication with ethanol which was done after 14 days of the CBD ligation. But the activities showed a less degree than group with the 14th day following CBD ligatioin. At the 1.5th and the 24th hours following the acute intoxication with ethanol which was done after 14 days of the CBD ligation, the liver mitochondrial GST activity decreased significantly, and the same was seen in the group with the 14th day following CBD ligatioin.

주정 중독 흰쥐에서 총담관결찰이 간 및 혈청의 Malate Dehydrogenase 활성에 미치는 영향

곽춘식,안광욱,임종술,김여희 啓明大學校 醫科大學 1991 계명의대학술지 Vol.10 No.3

The activities of the liver and serum malate dehydrogenase(MDH) were studied when cholestasis was induced by common bile duct ligation and chronic ethonic intoxication developed, or cholestasis following acute ethanol intoxiaction in hepatobiliary disease. The rats liver cytosolic MDH activities showed a significant increase in both chronically ethanol intoxicated groups and acutely ethanol intoxicated groups. But the rats liver mitochondrial MDH activities showed no significant changes in both chronically ethanol intoxiacted groups and acutely ethanol intoxicated groups. The groups that received common bile duct(CBD) ligation after being chronically intoxicated with ethanol showed a considerable decrease at the 2nd, 3rd, 7th and 14th days following the ligation in the liver cytosolic MDH activities. However, the activities showed a higher degree than groups of the CBD ligation. On the other hand, the liver mitochondrial MDH activities showed a significant decrease at the 3rd, 7th and 14th days following the ligation, but the activities showed a higher degree on the same time points than the groups only with the CBD ligation. At the 1.5th and 24th hours following the acute intoxication with ethanol done after 14days of the CBD ligation, the rats showed a slight decrease in the liver cytosolic and mitochondrial MDH activities than the goup only with the acute ethanol intoxication. However, the activities showed more remarkable increase than the goup sacrificed on the 14th day following the CBD ligation. The groups that receved CBD ligation after being chronically intoxicated with ethanol showed a dramatic increase at the 1st, 2nd, 3rd, 7th and 14th days following the ligation in the sera MDH activities. However, the activities showed a far higher degree than groups of the CBD ligation. For the goups of acute intoxication with ethanol done after 14 days of the CBD ligation, the sera MDH activities increased markedly, but the activities showed a higher degree than the goup with the 14th day following the CBD ligation. According to the above results, the liver cytosolic and mitochondrial MDH seems to be an enzyme which increases activities in both acute and chronic ethanol intoxication with cholestasis more than in cholestasis; the cause of the increase seems to be the development of biosynthesis. Especially, when the acuted and chronic ethanol intoxication with cholestsis occurred, the sera MDH are higher than in chlestasis because of increased liver cell damage, which causes the enzyme to leak into the blood in great quantity. Accordingly, these results will be the data supporting that alcoholic drink is enzymologically harmful in hepatobiliary disease.