High Level Production of human Protein Tyrosine Kinase-6 in Insect Cells Using Drosophila Peptidoglycan Recognition Protein-LB as a fusion protein

김슬기,김한이,우재성,조현수,정연진,이승택,하남출,Kim, Seul-Ki,Kim, Han-Ie,Woo, Jae-Sung,Cho, Hyun-Soo,Jung, Yun-Jin,Lee, Seung-Taek,Ha, Nam-Chul Korean Society of Life Science 2007 생명과학회지 Vol.17 No.2

단백질 티로신 kinase인 PTK6는 대부분의 유방암에서 과발현되며, 암세포의 증식만을 촉진하는데 역할을 한다. 이 연구에서 PTK6의 활성도메인을 초파리의 peptidoglycan recognition protein (PGRP) -LB 단백질을 퓨전파트너로 사용하여 바큘로바이러스 시스템을에서 과발현하는데 성공하였다. 우리는 PGRP-LB가 바큘로바이러스 시스템에서 잠재적으로 퓨전 단백질로 사용될 수 있는 가능성을 처음으로 발견하였다. 정제된 PTK6단백질은 기존의 박테리아에서 발현된 단백질보다 1.5배 높은 활성을 지녔다. 이 단백질은 PTK6의 분자기전 및 그것의 저해제 개발에 필수적인 결정 구조를 규명하는데 사용될 것이다. PTK6, an intracellular protein tyrosine kinase, is significantly overexpressed in a majority of breast cancers and has a role in promoting the proliferation of the cancer cells, but not of normal cells. Here, we report high-level production of the catalytic unit of PTK6 fused with Drosophila peptidoglycan recognition protein (PGRT)-LB, in the baculovirus system. We first found that the PGRP-LB was potentially useful as a fusion partner to increase the yield of heterologous protein in the baculovirus system. The purified recombinant protein exhibited a 1.5-fold activity with much higher yield than the bacterially-expressed protein. The protein expressed in the baculovirus system will be useful for the crystallization to determine its crystal structure helping understand the molecular mechanism of PTK6 and design its inhibitors.

군부대 내 부적응 병사에서 충동성과 혈청 지질과의 상관성

김슬기,강석훈,임명호,최종혁,이병용,Kim, Seul Ki,Kang, Suk-Hoon,Lim, Myung Ho,Choi, Jong hyuk,Lee, Byung yong 대한불안의학회 2012 대한불안의학회지 Vol.8 No.2

Objective : Previous studies reported a correlation between the low serum cholesterol level and impulsive behaviors. In this study, we investigate an association between the serum lipid levels and psychological parameters in maladaptive soldiers in the Korean Army. Methods : A total of ninety-six maladaptive subjects and thirty-six normal controls in the Korean army were evaluated with the Korean version of Barratt Impulsiveness Scale (K-BIS), Korean version of Beck Suicidal Ideation Scale (K- BSIS), Korean version of Beck Depression Inventory (K-BDI) and Korean version of Beck Anxiety Inventory (K-BAI). Serum total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) and high density lipoprotein (HDL) level were measured by overnight fasting blood sampling. Results : There were no significant differences between the groups in demographic characteristics. Serum total cholesterol levels (t=-2.209, p=0.032), triglyceride levels (t=-4.593, p<0.001), and LDL levels (t=-3.753, p=0.001) of maladaptive subjects were significantly lower than those of normal controls, and maladaptive subjects had higher K-BIS scores than normal controls (t=7.542, p<0.001). Negative correlation was found between LDL levels and non-planning impulsiveness in the maladaptive subjects (r=-0.253, p=0.013). LDL levels (${\beta}=-0.258$, p=0.008) and K-BDI scores (${\beta}=0.266$, p=0.043) emerged as significant predictors for non-planning impulsiveness. Conclusion : These results suggested that LDL level was associated with non-planning impulsiveness. These findings suggested that serum cholesterol levels might be available as a biological marker of impulsiveness. However, more large samples, longitudinal biological study and psychiatric evaluations should be needed to develop a preventive intervention for maladaptive male conscripts in the Korean army.

Taxonomic Notes on the Species of Hemerobiidae (Neuroptera) of Korea

김슬기,조수원,Kim, Seul-Ki,Cho, Soo-Won Korean Society of Applied Entomology 2011 한국응용곤충학회지 Vol.50 No.1

Micromus variegatus (풀잠자리목: 뱀잠자리붙이과)를 국내 미기록종으로 보고한다. 이와 함께 국외에 채집기록만 보고된 Hemerobius harmandinus, Sympherobius tessellatus 및 S. domesticus, 에 대한 간략한 기재, 성충 및 날개의 사진, 그리고 국명을 제시한다. 이로써 과거 보고된 4종과 함께 국내 뱀잠자리붙이과는 모두 8종으로 기록된다. Micromus variegatus (Neuroptera: Hemerobiidae) is reported here as new to South Korea. In addition, three other hemerobiid species recorded only the collection data, Hemerobius harmandinus, Sympherobius tessellatus and S. domesticus are also reported with a brief description, images of the adult and wings, and its Korean common name. With four previously reported species in Korea, there are eight species of the family Hemerobiidae from Korea.

미국 형사법상 범의(Mens Rea)와 엄격책임(Strict Liability)에 관한 고찰

김슬기 ( Seul Ki Kim ) 延世大學校 法科大學 法學硏究所 2009 法學硏究 Vol.19 No.2

특정한 범죄의 구성요건 요소에 대하여 범의가 없는 경우에도 책임을 부과하는 엄격책임은 우리 형법에서 찾을 수 없는 미국 형사법의 독특한 개념 중 하나이다. 엄격책임은 19세기 중엽 법실증주의 사조를 기반으로 급증하는 행정 형법에 대한 효과적인 법집행을 위하여 등장하였다. 그러나 사법적극주의와 법현실주의가 대두되는 20세기에 들어서 미연방대법원의 판례와 제정법은 범의 요건을 강조하여 점차 제한적인 범위에 한정하여 엄격책임을 인정하고 있다. 엄격책임 범죄는 대체적으로 벌금의 부과만이 가능한 경미 범죄로서, 일반 공중에 대한 법익 침해의 위험성이 존재하고, 범의의 입증이 사실상 곤란한 반면, 예상되는 기소 건수가 많다는 특징이 있다. 이에 대하여 적정절차 위반이라는 합헌성의 문제와 억제 효과 결여라는 합리성의 문제가 계속해서 제기되고 있으나 여전히 다수의 법률과 판례는 엄격책임의 존재 자체를 인정하고 있다. 엄격책임에 관한 검토는 상이한 법체계와 법이론으로 인하여 우리 법에의 도입이라는 측면에서는 의미가 크지 않겠지만, 새로운 규제 영역이 등장하고 있는 현실에서 행정 형법의 문제, 법률의 부지와 입증책임의 완화 등과 관련하여 형사정책적으로 시사하는 바가 있을 것으로 생각한다. One of the unique concepts of the American criminal law that cannot be found in Korean criminal law is strict liability, which imposes liability even in the absence of mens rea regarding one or more elements of a crime. Strict liability had initially appeared for the effective execution of regulatory criminal law, which had proliferated based on legal positivism in mid-19th century. However, with the rise of judicial activism and legal realism in the 20th century, the cases of the Supreme Court and statutory law, emphasizing the requirement of mens rea, are gradually confining strict liability within narrower limits. Strict liability offenses are generally violations for which only fines can be imposed. These crimes are characterized by danger to public welfare, practical difficulty of proving the element of mens rea and the abundance of expected prosecution. Although the issue of constitutionality regarding the violation of due process and the issue of practicality questioning whether there is a deterrent effect are being raised, much of the law and many cases still acknowledge the existence of strict liability. Although the discussion of adopting strict liability in Korean criminal law does not hold much significance because of different legal system and doctrine, strict liability is suggestive to our discussion about newly arisenissues such as administrative penalty, ignorance of law and burden of proof.

전력소비자 수요관리용 전지전력저장시스템의 적정 가격 산정

김슬기(Seul-Ki Kim),조경희(Kyeong-Hee Cho),김종율(Jong-Yul Kim),김응상(Eung-Sang Kim) 대한전기학회 2013 전기학회논문지 Vol.62 No.10

The paper estimated the reasonable market price of lead-acid battery energy storage system (BESS) intended for demand management of electricity customers. As time-of-use (TOU) tariffs have extended to a larger number of customers and gaps in the peak and off-peak rates have gradually risen, deployment of BESS has been highly needed. However, immature engineering techniques, lack of field experiences and high initial investment cost have been barriers to opening up ESS markets. This paper assessed electricity cost that BESS operation could save for customers and, based on the possible cost savings, estimated reasonable prices at which BESSs could become a more prospective option for demand management of customers. Battery scheduling was optimized to maximize the electricity cost savings that BESS would possibly achieve under TOU tariffs conditions. Basic economic factors such as payback period and return on investment were calculated to determine reasonable market prices. Actual load data of 12 industrial customers were used for case studies.

계통연계형 풍력, 태양광 및 축전지 하이브리드 시스템의 출력제어 및 동특성 해석

김슬기(Seul-Ki Kim),全鎭洪(Jin-Hong Jeon),趙昌熙(Chang-Hee Cho),安鍾普(Jong-Bo Ahn) 대한전기학회 2007 전기학회논문지 Vol.56 No.2

Most conventional hybrid systems using renewable energy sources have been applied for stand-alone operation, but Utility-interface may be an useful and viable option for hybrid systems. Grid-connected operation may have benefits such as reduced losses in power system distribution, utility support in demand side management, and peak load shaving. This paper addresses power control and dynamic performance of a grid-connected PV/wind/BESS hybrid system. At all times the PV array and the wind turbine are individually controlled to generate the maximum energy from given weather conditions. The battery energy storage system (BESS) charges or discharges the battery depending on energy gap between grid invertger generation and production from the PV and wind system. The BESS should be also controlled without too frequently repeated shifts in operation mode, charging or discharging. The grid inverter regulates the generated power injection into the grid. Different control schemes of the grid inverter are presented for different operation modes, which include normal operation, power dispatching, and power smoothing. Simulation results demonstrate that the effectiveness of the proposed power control schemes for the grid-interactive hybrid system.

인포테인먼트 관점에서 본 창의적 인터페이스 디자인 연구

김슬기(Kim Seul Ki),윤주현(Juhyune Eune) 한국디자인학회 2009 한국디자인학회 학술발표대회 논문집 Vol.2009 No.5

2022 비버챌린지 결과를 중심으로 한 참여 현황 및 문항별 정답률 분석

김슬기(Seul ki Kim),김도용(Do yong Kim),전용주(Yong ju Jeon),정상수(Sang su Jeong),김동윤(Dong yoon Kim) 한국컴퓨터교육학회 2023 한국컴퓨터교육학회 학술발표대회논문집 Vol.27 No.1

성폭행 피해 환자 사례에 대한 분석

김슬기 ( Seul Ki Kim ),이민영 ( Min Young Lee ),최상준 ( Sang Joon Choi ),안태규 ( Tae Gyu Ahn ),서정이 ( Jeong A Seo ),정효영 ( Hyo Young Jeong ) 대한산부인과학회 2011 Obstetrics & Gynecology Science Vol.54 No.1

목적: 성폭행으로 피해자의 병원방문 시간, 사건 발생 추정 시간, 장소, 피해자와의 관계, 정자 검출여부, 진료 후 추적관찰 여부를 알아보고자 하였다. 연구방법: 2008년 1월 1일부터 2008년 12월 31일까지 광주 원스톱 지원센터를 방문하여 산부인과 진료 시행한 여자환자 204명을 대상으로 후향적으로 조사하였다. 결과: 성폭행 피해자는 4, 6월에 가장 많았고 92.2%가 형사사건화 되었다. 가장 높은 발생 추정시간 및 원스톱 센터 방문시간은 각각 24시부터 3시, 24시부터 9시였다. 피해 장소는 피해자의 집이 65건(31.9%), 숙박업소가 53건(26.0%)였다. 가해자가 비 면식범인 경우가 113명(55.4%), 면식범인 경우가 91명(44.6%)였다. 성폭행 발생 후 병원 방문까지 소요시간은 13세 이하가 172시간, 14세부터 16세가 96시간, 17세부터 19세가 39시간, 20세 이상이 24시간으로 연령이 낮은 경우에 병원까지의 소요시간이 높았다. 정자채취를 시행한 154명 중 27명(17.5%)에서 정자가 검출되었고 이는 모두 72시간 이전에 검사를 시행한 경우이다. 피해자의 피해여부를 살펴보면 생식기 외상을 입은 피해자가 18명(8.9%), 추후 정신과 진료를 받은 피해자가 8명(3.9%), 갈비뼈 골절 및 항문외상, 아래턱 골절을 입은 피해자가 1명(0.5%) 있었다. 또한 성폭행으로 임신한 피해자가 2명(1%) 있었다. 진료 후 추적 관찰률은 11.3%였으며 추적관찰을 한 집단과 하지 않은 집단 사이에 나이와 이전 성경험 여부에 따라 비교하였으나 유의한 차이를 보이지 않았다. 결론: 성폭행 피해자를 진료하는 의사는 변화하는 시대의 흐름에 따른 피해자의 상황과 정자채취의 확률을 높일 수 있는 조건을 파악하고 피해자들의 향후 신체적 정신적 후유증을 예방하기 위해 추적관찰을 효과적으로 교육할 수 있어야 한다. Objective: The purpose of this study was to evaluate the time when victims arrived at the hospital, the time of the attack, location, social relationship with assailants, detection of sperm, and whether a follow-up on sexual assault victims was possible. Methods: Two hundred four sexual assault victims who visited the Gwangju One-stop Center and received gynecologic treatment from January 1st, 2008 to December 31st, 2008 were analyzed retrospectively. Results: Most victims were raped in April and June. Most attacks happened between midnight and 3 AM. Victims visited the hospital between midnight and 9 AM. The most frequent locations of assault were the victim``s house (65 cases, 31.9%). 55.4% of assailants were strangers to the victims. The mean interval time from rape to visit to the hospital by age was 172 hours (for victims≤13 years old) and 24 hours (≥20 years old). The detection rate of sperm in the vagina was 17.5% (154 victims) and the follow up rate was 11.3%. The injuries of the victims were as follows; 18 (8.9%) patients had genital injuries, 8 (3.9%) patients underwent psychological treatment, 1 (0.5%) patient had rib and mandible fracture with anal injury, and 2 (1%) patients became pregnant after the rape. Conclusion: The doctors who treat sexual assault victims must consider the situation of the victims and the conditions for increasing the probability of sperm detection. We should also educate the patients so that they come back for follow-up checks for preventing physical and psychological complications.

디즈니 뮤지컬 〈노틀담의 꼽추〉의 라이트모티브 분석

김슬기(Seul-Ki Kim),이은혜(Eun-Hye Lee) 한국엔터테인먼트산업학회 2023 한국엔터테인먼트산업학회 학술대회 논문집 Vol.2023 No.6