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      • Purification and Amino-Terminal Amino Acid Sequence of a Ribonuclease from Bovine Seminal Plasma

        김성기,Kim, Sung-Kih 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.1

        소의 정액으로부터 리보뉴클리아제를 50-70% 포화 황산암모늄에 의한 침전 반응과, concanavalin A-Sepharose 4B, DEAE-셀룰로오스, agarose-5'-(4-aminophenylphos pho)-uridine 2'(3')-phosphate, Sephadex G-75 등에 의한 크로마토그라피로 정제 하였 다. 정제된 효소의 균일성은 폴리아크릴아미드 젤 전기영동으로 확인하였다. 이 효소의 분자량은 젤 여과법으로 12,500임을 알았다. 또 이 효소의 활성은 $Na^+$, $K^+$, $Mg^{2+}$, $Fe^{2+}$ 및 EDTA에 의하여 증가 되었고, $Ca^{2+}$, $Mn^{2+}$, $Zn^{2+}$ 및 $Cu^{2+}$에 의하여 억제되었다. 정제된 효소는 poly(C) 및 효모 RNA 보다는 poly(U)에 대하여 더 큰 친화성을 나타내었다. 정제된 효소 단백질 N-말단 아미노산 배열을, 아미노산 배열 자동분석기로 31번째 잔기까지 결정하였다. 그 결과는 다음과 같다. $\array{1\\Val}$ Asp Ser Lys $\array{5\\Gly}$ Gly Lys Tyr Gln $\array{10\\Arg}$ Glu His Met Asp $\array{15\\Leu}$ Asp Ser Leu Pro $\array{20\\Ala}$ Ala Val Gly Thr $\array{25\\Tyr}$Cys Asp Ala Met $\array{30\\Lys}$ Leu 이상의 결과로부터 소의 정액에서 정제한 리보뉴클리아제는 전립선-특이 효소임을 알 수 있었다. A ribonuclease has been purified to homogeneity from bovine seminal plasma by precipitation with 50-70% saturated ammonium sulfate, followed by chromatographies on concanavalin A-Sepharose 4B, DEAE-cellulose, agarose-5'-(4-aminophenylphospho)-uridine 2'(3')-phosphate, and Sephadex G-75. The homogeneity of this ribonuclease was confirmed by polyacrylamide gel electrophoresis. Molecular weight for this purified ribonuclease was 12,500 as estimated by gel filtration. The enzyme activity was activated by $Na^+$, $K^+$, $Mg^{2+}$, $Ba^{2+}$, $Fe^{2+}$ and EDTA and inhibited by $Ca^{2+}$, $Mn^{2+}$, $Zn^{2+}$, and $Cu^{2+}$ The purified ribonuclease preferentially hydrolyzed poly(U) over poly(C) and yeast RNA. The N-terminal amino acid sequence of 31 residues for the purified seminal ribonuclease has been determined by automatic sequencing of the native protein. The following sequence was deduced: $\array{1\\Val}$ Asp Ser Lys $\array{5\\Gly}$ Gly Lys Tyr Gln $\array{10\\Arg}$ Glu His Met Asp $\array{15\\Leu}$ Asp Ser Leu Pro $\array{20\\Ala$ Ala Val Gly Thr $\array{25\\Tyr}$ Cys Asp Ala Met $\array{20\\Lys}$ Leu These results suggested that the purified bovine seminal ribonuclease was a prostate-specific enzyme.

      • KCI등재

        필수 서비스 생존성 향상을 위한 자원 재할당 기법

        김성기,민병준,최중섭,김홍근,Kim, Sung-Ki,Min, Byoung-Joon,Choi, Joong-Sup,Kim, Hong-Geun 한국정보처리학회 2003 정보처리학회논문지 C : 정보통신,정보보안 Vol.10 No.1

        새로운 방법의 시스템 공격에 대해서도 필수 서비스의 생존성을 보장하기 위해서는 서비스가 필요로 하는 중요 자원을 식별하고 위급 상황에 적절히 대응하는 방안이 필요하다. 본 논문에서는 침입 감내 시스템 구축을 위한 핵심 기술의 하나인 동적 자원 재할당 기법을 제시한다. 이 기법에서는 선택된 편수 서비스에 대해 해당 호스트 내에서 자원을 재할당하여 침입이 발생한 후에도 필수 서비스가 생존할 수 있도록 한다. 테스트베드를 구축하여 실험을 실시한 결과 본 자원 재할당 기법의 타당성을 입증할 수 있었다 향후 이 기법을 침입 탐지 시스템과 접목 시키면 매우 효과적인 공격 대응 방안이 될 것이다. In order to guarantee the survivability of essential services against attacks based on new methodology, we need a solution to recognize important resources for the services and to adapt the urgent situation properly. In this paper, we present a dynamic resource reallocation scheme which is one of the core technologies for the construction of intrusion tolerant systems. By means of resource reallocation within a host, this scheme enables selected essential services to survive even after the occurrence if a system attack. Experimental result obtained on a test-bed reveals the validity of the proposed scheme for resource reallocation. This scheme may work together with IDS (Intrusion Detection System) to produce more effective responsive mechanisms against attacks.

      • 광배근 유리 피판술을 이용한 두피 및 두개골 결손의 재건

        김성기,노시균,이내호,양경무,Kim, Seong-Ki,Roh, Si-Gyun,Lee, Nae-Ho,Yang, Kyung-Moo 대한미세수술학회 2013 Archives of reconstructive microsurgery Vol.22 No.1

        Purpose: Reconstruction of scalp and calvarial defects should provide both aesthetic and functional aspects. The inelastic nature of the scalp and previous surgery or radiation preclude the use of primary closure or a local flap. With development of microsurgical technique, a free tissue transfer is a good option. We use the latissimus dorsi myocutaneous free flap for reconstruction. Materials and Methods: A review of all latissimus dorsi free flap reconstructions performed in nine patients from 2009 to 2012 was conducted. There were six males and three females, ranging in age from seven to 69 years, and nine different regions, including five temporal regions, two occipital regions, and two frontoparietal regions. The flaps ranged in size from $9.0{\times}10.0cm$ to $14.0{\times}15.0cm$. Recipient vessels available for microanastomosis were most often the superficial temporal vessels and two patients had anastomoses to the external carotid artery and internal jugular vein. Results: All flaps survived postoperatively. With a median follow-up period of 14 months, no major complications were noted. However, two patients developed minor wound dehiscence, and a hematoma was observed in one patient. Conclusion: We performed the latissimus dorsi myocutaneous free flap reconstruction, which is one of the most popular reconstructive methods. The latissimus dorsi myocutaneous free flap reconstruction has been proven successful in our patients with satisfactory results. During the long term follow-up period, even though depressions were observed on the defect area in some patients, they were treated successfully with cranioplasty. Therefore, we recommend the latissimus dorsi myocutaneous free flap for reconstruction of scalp and calvarial defects.

      • KCI등재

        절차적 추상화의 분류와 다형성

        김성기,Kim, Sung-Ki 한국정보처리학회 2003 정보처리학회논문지 A Vol.10 No.1

        소프트웨어의 개발, 유지보수 및 확장을 용이하기 하기 위하여 프로그래밍 언어에 여러 추상화 기법이 도입되었다. 그러나 추상화와 연관된 여러 개념과 용어가 통일성이 있게 정의되지 못하므로 인하여 관점에 따라 다르게 설명되기도 한다. 특히 추상화를 가장 강력한 기반으로 하여 태동된 객체 지향 패러다임에서 이러한 혼란은 아직도 계속되고 있는 실정이다. 본 논문에서는 프로그래밍 언어에서 가장 중요한 추상화의 하나인 절차적 추상화를 분석하고 이에 대한 유형을 살펴본다. 이를 통하여 절차적 추상화가 지금까지 일반적으로 인식되어온 '처리과정을 기능으로 바꾸는 추상화'라는 기본적인 추상화의 개념 외에도 '추상화에 대한 추상화'의 개념이 내포된 고수준의 추상화도 포함되어 있음을 밝힌다. 또한 절차적 추상화와 관련된 다형성을 추상화의 관점에서 설명한다. 이러한 분석과 고찰을 통하여 절차적 추상화에 대한 수준 높은 이해가 이루어질 수 있을 것이다. Many abstraction techniques are introduced in programming languages in order to facilitate the development, maintenance and extension of softwares. But several concepts and terms related to abstraction have not been uniformly defined and have been explained differently. Expecially in object-oriented paradigm strongly based on abstraction common consensus was not derived. In this paper, we analyse procedural abstraction, propose the taxonomy of procedural abstraction and show that procedural abstraction contains the concept of abstraction on abstraction in addition to the transformation of procedures into abstraction. We also explain polymorphism in the view of abstraction. These analysis and consideration will make it possible to understand procedural abstraction more intensively.

      • KCI등재

        R . F . PACD에 의하여 증착된 TiN의 경도와 밀착력에 미치는 모재 경도의 영향

        김성기,김문일 ( S . K . Kim,M . I . Kim ) 한국열처리공학회 1991 熱處理工學會誌 Vol.4 No.1

        This study was to investigate the influence of the substrate hardness on the hardness and adhesion of TiN thin film deposited by R. F. PACVD. Although the substrate hardness changed, chemical composition, stoichiometry and structure of TiN thin film did not change. ISE index was 1.96-1.99 for the substrate and was 1.57-1.79 for TiN thin film. And ISE index of TiN thin film was inverse proportion to the substrate hardness. When the substrate hardness was low, TiN thin film had many cracks around the indentation. But as the substrate hardness increased, TiN thin film had a few cracks and the deformation was limited within indentation. In having measured the adhesion of TiN thin film by SAT, the critical load(Lc) generally increased as the substrate hardness decreased.

      • KCI등재

        수부의 고압 분사 손상

        김성기,노시균,이내호,양경무,Kim, Seong-Ki,Roh, Si-Gyun,Lee, Nae-Ho,Yang, Kyung-Moo 대한성형외과학회 2010 Archives of Plastic Surgery Vol.37 No.3

        Purpose: High-pressure injection injury is caused by accidental injection of the high-pressure injection devices in industry. The initial benign appearance of the wound fools patients into delays in an adequate treatment. And it can result in disastrous outcomes such as necrosis and amputation. To avoid the poor prognosis, the injuries require a prompt surgical intervention. The purpose of this article is to recognize the poor outcome of the highpressure injection injury and to introduce an adequate treatment in need. Methods: We have 4 cases of the high-pressure injection injuries in the hand from April, 2005 to March, 2009. Average age is 39 years (30 - 49 years old), 2 cases are the palm of dominant hand, 1 case is the thumb of dominant hand, and 1 case is the palm of non-dominant hand, respectively. We followed up these patients for 20 months on average. In 3 cases, the immediate, aggressive surgical intervention was carried out, but the other one was delayed in early adequate treatment. The wounds were covered by local advancement flap, anterolateral thigh free flap, conservative treatment with antibiotics and dressing. Results: No pathogens after culture were found nor any findings of fracture in imaging study. Conservative treatment, local advancement flap and anterolateral thigh free flap for the open wound resulted in a desirable aesthetic outcome. In a long-term follow up, functional capability of the patient was also satisfactory. Conclusion: Upon initial evaluation, most high-pressure injection injuries present as innocuous wounds with very few symptoms and result in delaying the proper management. And the majority of high-pressure injection injuries will produce significant morbidity to the hand, amputation. And the initial aggressive surgical debridement was needed to prevent the poor outcome. The key to success in treating high-pressure injection injuries of the hand is the prompt aggressive surgical intervention.

      • '지금 여기서' 탈식민주의 읽기

        김성기,Kim, Seong-Gi 대한출판문화협회 1998 출판저널 Vol.243 No.-

        탈식민주의의 주된 논지는 유럽 제국주의의 붕괴라는 공통의 경험적 자료에서 출발한다. 변방에 속한 것은 식민지 본국에 속한 것에 대해, 즉 주변은 중심에 대해 반감을 갖게 되므로 탈식민지 문화의 모는 경험은 전복을 지향하는 경향을 띤다.

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