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Quantitative and Rapid Analysis of Transglutaminase Activity Using Protein Arrays in Mammalian Cells
권미혜,정재완,정세희,Jin-Young Park,김영명,Kwon-Soo Ha 한국분자세포생물학회 2009 Molecules and cells Vol.27 No.3
We developed a novel on-chip activity assay using protein arrays for quantitative and rapid analysis of transglutaminase activity in mammalian cells. Transglutaminases are a family of Ca2+-dependent enzymes involved in cell regulation as well as human diseases such as neurodegenerative disorders, inflammatory diseases and tumor progression. We fabricated the protein arrays by immobilizing N,N′-dimethylcasein (a substrate) on the amine surface of the arrays. We initiated transamidating reaction on the protein arrays and determined the transglutaminase activity by analyzing the fluorescence intensity of biotinylated casein. The on-chip transglutaminase activity assay was proved to be much more sensitive than the [3H]putrescineincorporation assay. We successfully applied the on-chip assay to a rapid and quantitative analysis of the transglutaminase activity in all-trans retinoic acid-treated NIH 3T3 and SH-SY5Y cells. In addition, the on-chip transglutaminase activity assay was sufficiently sensitive to determine the transglutaminase activity in eleven mammalian cell lines. Thus, this novel on-chip transglutaminase activity assay was confirmed to be a sensitive and high-throughput approach to investigating the roles of transglutaminase in cellular signaling, and, moreover, it is likely to have a strong potential for monitoring human diseases.
권미혜,윤지현,김은혜,이지영,윤성우,Kwon, Mi Hye,Yoon, Jee-Hyun,Kim, Eun Hye,Lee, Jee Young,Yoon, Seong Woo 대한암한의학회 2020 大韓癌韓醫學會誌 Vol.25 No.1
Objective : Radiodermatitis is a common sequelae in 95% of patients receiving radiation therapy, which is important to be well managed as it can affect the patient's quality of life as well as the cancer treatment schedule. The aim of this study is to review and summarize the interventions available for the treatment of acute radiodermatitis, including traditional Korean medicine, and to propose treatment algorithms for clinicians. Methods : To collect studies about managements for radiodermatitis, domestic and foreign database were used such as Korean journal of traditional knowledge portal (KTKP), Korean studies information service system (KISS), national discovery or science leaders (NDSL), and oriental medicine advance searching integrated system (OASIS), Pubmed, Google scholar and Scopus. Results : Thirty-two studies were selected. There were nine studies on usual care and dressing, eleven studies on chemical agents, two studies on biological preparations, and ten studies on herbal medicines. Conclusion : Hygienic options and dressings have proved to be useful in the management of radiodermatitis. Chemical agents such as corticosteroid, statin, and topical antibiotic agent have proved to alleviate symptoms and severity, regenerate damaged skin, and prevent secondary infection. In biological preparations, EGF (epidermal growth factor) and GM-CSF (granulocyte-macrophage colony-stimulating factor) could be used to protect skin and prevent radiodermatitis. For herbal medicines, Calendula, catechin, β-sitosterol, and Jaungo (紫雲膏) may be effective for symptoms including pain, itching, and burning sensation induced by radiation therapy. Because of some research with conflicting results, further studies are needed to propose an algorithm for more optimal treatments.
권미혜,장하림,이혜리,이의주,Kwon, Mi Hye,Jang, Halim,Lee, Hyeri,Lee, Eui Ju 사상체질의학회 2021 사상체질의학회지 Vol.33 No.4
Objectives This study was aimed to report a significant improvement of chronic diarrhea patient, diagnosed as 'Taeeum type liver heat-lung dryness pattern' based on Sasang medicine. Methods The patient had treatment with Sasang constitutional medicine. The treatment was executed three times everyday from August 27th to September 3rd and two times everyday from September 3rd to September 16th of 2021. Clinical improvement was evaluated with Bristol stool form scale, the degree of abdominal discomfort and nausea compared to the day of hospitalization and the number of defecations and nocturia per day. Results The symptoms of diarrhea, abdominal discomfort and nausea were improved. The number of night urination was decreased from 3-4 times to once a night. Conclusion After the treatment with Sasang constitutional medicine, diarrhea, abdominal discomfort, nausea and the general condition of the patient also improved.
감각을 활용한 미술교육이 만 2세 영아의 정서지능에 미치는 효과
권미혜(Kwon, Mi-hye),방혜경(Bang, Hye-kyung),이숙재(Lee, Sook-jae) 한국어린이미디어학회 2012 어린이미디어연구 Vol.11 No.1
본 연구는 감각을 활용한 미술교육이 만2세 영아의 정서지능에 미치는 효과를 알아보기 위하여 어린이집을 다니는 24개월에서 32개월 영아를 대상으로 13명의 실험집단과 13명의 통제집단을 선정하여 실험집단에게 주2회 8주 총 16회 실시하였다. Mayer와 Salovey (1996)의 정서지능모형을 김효진 (2005) 이 수정 제작한 “교사용 관찰 검사지” 평정척도를 사용하여 사전-사후 검사를 실시한 후 공변량 분석과 매회기마다 녹화를 통해 관찰분석을 하였으며 그 결과는 다음과 같다. 감각을 활용한 미술교육이 만2세 영아의 정서지능 향상에 효과가 있는 것으로 나타났고 하위영역별로는 자기 정서의 인식ㆍ표현능력, 감정조절 및 충동억제, 자기정서이용, 타인 정서의 인식 및 배려, 교사와의 관계, 또래와의 관계에는 통계적으로 유의미한 차이가 있었으며 감각을 활용한 미술교육이 영아의 정서지능 증진에 효과적인 자료가 될 수 있음을 알 수 있다. purpose of this study is to examine the effects of art education using sensory experiences on emotional intelligence of infants who reached the age of two. The research participants of this study consist of 13 infants in an experimental group and 13 infants in a control group. Each infant from 24 to 32 months of age has attended a child care center. Experiments were conducted sixteen times for eight weeks, twice a week. To accomplish the purpose of this study, the data were collected by the rating scale of “Observation Instrument for Teachers“ modified by Hyojin Kim (2005) from the emotional intelligence model of Mayer and Salovey (1996). The collected data were treated through videotaped footage at each session by using the pre-post tests, analysis of covariance, and observation analysis. The results of this study are as follows: Art activities using sensory experiences have the effects on the improvement of the emotional intelligence of infants who reached the age of two. Especially the researchers have found a statistically significant difference on the awareness and expression of self-emotion, emotion control and restraint of impulse, use of self-emotion, recognition and consideration of others’ emotion, relationship with teachers, and relationship with peers. Therefore, the result of this study implies that art activities using sensory experiences can be effective materials for the improvement of the infants’ emotional intelligence.
권미혜(Mi Hye Kwon),김광일(Kwang Il Kim),권혁주(Hyuck Ju Kwon),정진규(Jin Kyu Jung) 대한설비공학회 2018 대한설비공학회 학술발표대회논문집 Vol.2018 No.11
In this paper, the performance prediction model of the automotive oil cooler was developed using the Modelica language, and its applicability to actual work was confirmed. In particular, the program was completed by applying Dymola & Modelon Library as one-dimensional analysis approach. Numerical analysis results from Modelica based performance prediction program are also compared with experimental results. The results are well matched within +/-3%, and can be usefully used to establish product development direction at the initial design stage. And also this program can be usefully used in RFI/RFQ stage quickly. Modelica-based TOC performance prediction codes are also expanded to stacked plate type and air-cooled type.