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急性骨髓性 白血病에서의 試驗管內 集落細胞 形成에 관한 연구
고석만,조덕연,박철신,강원권,김민범,김삼용,노흥규 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2
To evaluate the in vitro granulocyte-macrophage colony formation in acute myfloblastic leukemia and the prognostic implications of these results, the author performed the in vitro agar culture of bone marrow cells in 10 patients with acute myeloblastic leukemia (AML) and 5 control subjects. Culture medium was composed of 20% fetal calf serum(FCS), 50% Iscove's medium, 0.3% agar, 10% colony stimulation factor (CSF), and 2 × 10 exp (5) cells/ml. Human placental conditioned medium (HPCM) and phytohemagglutinin-leukocyte conditioned medium (PHA-LCM) were used as colony stimulating factor. Colony counting was done on 7th day of culture. Colony was defi ned as containg 20 or more cells, and cluster was defined as containing 3-19 cells. The results were as followings, 1. In control subjects, the number of clusters formed was 3-47/2 × 10 exp (5) cells (20±19)and that of colonies was 5-24/2 × 10 exp (5) cells (14±9) when stimulated with HPCM. When stimulated with PHA-LCM, the number of clusters formed 5-39/2 × 10 exp (5) cells(18±16) and that of colonies was 6-13/2 × 10 exp (5) cells (9±3). 2. In AML patients, 3 groups were recognized according to pattern of colony formation: 1) non-forming 2) cluster forming 3) both cluster and colony forming. Of 10 cases, 5 cases Were `non-forming', 2 cases were `cluster forming'; and 3cases wer cluster and `colony forming'; 3. All 5 cases 'non-forming' cases, one of 2 cases of `cluster forming'; and none of 3 cases of `cluster and colony forming' achieved complete remission. So, there was significa nt difference in remission rate in the different growth types. These results suggest that granulopoiesis in AML patients is impaired and the pattern of in vitro CFU-L(colony forming unit-leukemia) formation has prognostic significance.
고석만,김장형,이성철 濟州大學校 工科大學 産業技術硏究所 1998 尖端技術硏究所論文集 Vol.9 No.2
The goal of this study is to establish a social welfare information system in order to provide a way of improving the quality of life of society's under-privileged people, such as the old and the disabled, by using highly-developed information and communication techniques. For easy and convenient user interface. proposed system is implemented by the pull down graphic menu using Visual Basic.
급성골수성백혈병에서의 시험관내 집락세포 형성에 관한 연구
고석만,김종완,박철신,조덕연,김상용,노흥규 충남대학교부설 생명공학연구소 1991 생물공학연구지 Vol.1 No.-
To evaluate the in vitro granulocyte-macrophage colony formation in acute myeloblastic leukemia and the prognostic implications of these results, the author performed the in vitro agar culture of bone marrow cells in 15 patients with acute myeloblastic leukemia(AML) and 5 control subjects. Culture medium was composed of 20% fetal calf serum(FCS), 50% Iscove's medium, 0.3% agar, 10% colony stimulation factor(CSF), and 2×10 exp (5) cells/㎖. Human placental conditioned medium(HPCM) and phytohemagglutinin-leukocyte conditioned medium(PHA-LCM) were used as coloy stimulating factor. Colony counting was done on 7th day of culture. Colony was defined as containing 20 or more cells, and cluster was defined as containing 3-19 cells. The results were as followings, 1. In control subjects, the number of clusters formed was 3-47/2×10 exp (5) cells(20±19) and that of colonies was 5-24/2×10 exp (5) cells(14±9) when stimulated with HPCM. When stimulated with PHA-LCM, the number of clusters formed was 5-39/2×10 exp (5) cells(18±6) and that of colonies was 6-13/2×10 exp (5) cells(9±3). 2. In AML patients, 3 groups were recognized according to pattern of colony formation : 1) non-forming, 2) cluster forming, 3) both cluster and colony forming. Of the 15 cases, 10 cases were 'non-forming', 2 cases were 'cluster forming,' and 3 cases were 'cluster and colony forming'. 3. 8 of 10 cases of 'non-forming' cases, one of 2 cases of 'cluster forming,' and none of 3 cases of 'cluster and colony forming' achieved complete remission. So, there was significant difference in remission rate in the different growth types. These results suggest that granulopoiesis in AML patients is impaired and the pattern of in vitro CFU-L(colony forming unit-leukemia) formation has prognostic significance.
急性骨髓性 白血症에서의 試驗管內 集落細胞 形成에 관한 연구
고석만,조덕연,박철신,강원권,김민범,김삼용,노흥규 忠南大學校 癌共同硏究所 1991 癌共同硏究所 硏究誌 Vol.1 No.1
To evaluate the in vitro granulocyte-macrophage colony formation in acute myeloblastic leukemia and the prognostic implications of these results, the author performed the in vitro agar culture of bone marrow cells in 10 patients with acute myeloblastic leukemia (AML) and 5 control subjects. Culture medium was composed of 20% fetal calf serum(FCS), 50% Iscove's medium, 0.3% agar, 10% colony stimulation factor (CSF), and 2×105 cells/ml. Human placental conditioned medium (HPCM) and phytohemagglutinin-leukocyte conditioned medium (PHA-LCM) were used as colony stimulating factor. Colony counting was done on 7th day of culture. Colony was defined as containg 20 or more cells, and cluster was defined as containing 3 - 19 cells. The results were as followings, 1. In control subjects, the number of clusters formed was 3-47/2×10^(5) cells (20±19)and that of colonies was 5-24/2×10^(5) cells (14±9) when stimulated with HPCM. When stimulated with PHA-LCM, the number of clusters formed 5-39/2×10^(5) cells(18±16) and that of colonies was 6-13/2×10^(5) cells (9±3). 2. In AML patients, 3 groups were recognized according to pattern of colony formation : 1) non-forming 2) cluster forming 3) both cluster and colony forming. Of 10 cases, 5 cases were 'non-forming', 2 cases were 'cluster forming'; and 3cases wer cluster and 'colony forming'; 3. All 5 cases 'non-forming' cases, one of 2 cases of 'cluster forming'; and none of 3 cases of 'cluster and colony forming' achieved complete remission. So, there was significant difference in remission rate in the different growth types. These results suggest that granulopoiesis in AML patients is impaired and the pattern of in vitro CFU-L(colony forming unit-leukemia) formation has prognostic significance.
이성철,고석만,김장형 濟州大學校 産業技術硏究所 1998 산업기술연구소논문집 Vol.9 No.2
The goal of this study is to establish a social welfare information system in order to provide a way of improving the quality of life of society's under-privileged people. such as the old and the disabled. by using highly-developed information and communication techniques. For easy and convenient user interface, proposed system is implemented by the pull down graphic menu using Visual Basic.