전처리 방법이 유산균수 측정에 미치는 영향

강주영,이서현,박은지,김중범,Kang, Ju-Yeong,Lee, Seo-Hyun,Park, Eun-Ji,Kim, Jung-Beom 한국식품영양학회 2019 韓國食品營養學會誌 Vol.32 No.5

The purpose of this study was to analyze the effects of the pre-treatment method on the measurement of probiotic cell counts. The probiotic cell count was not significantly different in the pre-treatment method such as experimenters, diluted solution, medium, and homogenization duration. The mean value of probiotic cell count with capsule was $2.2{\times}10^{10}{\pm}9.5{\times}10^9CFU/g$. This probiotic cell count was converted into $2.8{\times}10^{10}{\pm}1.2{\times}10^{10}CFU/g$ based on the net weight. The mean value of probiotic cell count without capsules was $4.3{\times}10^{10}{\pm}1.8{\times}10^{10}CFU/g$. As a result of this comparison, probiotic cell count showed significant difference with and without capsules. Thus, it is suggested that the probiotic cell count is measured by removing the capsule in capsule probiotics.

물 중 일반세균 정량에 대한 배지법과 건조필름법의 상관관계

강주영 ( Ju Yeong Kang ),조아현 ( Ah Hyeon Jo ),박은지 ( Eun Ji Park ),이한철 ( Han Cheol Lee ),박민지 ( Min Ji Park ),한진섭 ( Jin Sub Han ),강성규 ( Seong Gyu Kang ),김중범 ( Jung Beom Kim ) 한국물환경학회(구 한국수질보전학회) 2020 한국물환경학회지 Vol.36 No.2

The quantitative accuracy of dry rehydratable film method was compared with the standard culture method to analyze the applicability of dry rehydratable film in the total aerobic bacteria test for water. The materials used in this study were 500 cases of water and were tested according to the Korean official test method for drinking water. The mean value of the total aerobic bacteria in plate count agar(PCA) and MC-Media Pad AC(MAC) were 2.6 ± 1.1 Log cfu/mL and 2.6 ± 1.2 Log cfu/mL. The p value and correlation coefficient(R²) were 0.933 and 0.9985 between the PCA and MAC, respectively, indicating no significant difference and very high correlation. According to the water types, the p value and R² were 0.887 and 0.9911 in the PCA and MAC. In the purified water, the p value was 0.973 and R² was 0.9934 in the PCA and MAC of other water. In each type of water, there were no significant differences between the PCA and MAC, and the correlation was very high. Thus, it is suggested that the MAC would be available for total aerobic bacteria test for water.

DNA Barcode를 이용한 수산가공품 원재료 진위판별

박은지,강주영,이한철,박민지,양지영,신지영,김군도,김종오,서용배,김중범,Park, Eun-Ji,Kang, Ju-Yeong,Lee, Han-Cheol,Park, Min-Ji,Yang, Ji-Young,Shin, Ji-Young,Kim, Gun-Do,Kim, Jong-Oh,Seo, Yong-Bae,Kim, Jung-Beom 한국식품위생안전성학회 2021 한국식품위생안전성학회지 Vol.36 No.4

본 연구에서는 DNA barcode 시험법을 이용하여 시중 유통 중인 도미와 옥돔 수산가공식품의 위변조현황을 분석하였다. 참돔 12건, 돌돔 4건, 황돔 7건, 감성돔 2건, 나일틸라피아 7건, 옥돔 6건, 옥두어 8건 총 46건의 시료에 대해 실험을 진행하였다. 생물 종 판별에 주로 이용되는 mitochondrial DNA의 COX I (cytochrome C oxidase subunit I) 유전자 영역을 분석하여 원재료의 종을 판정하였다. NCBI에서 제공하는 BLAST Search 프로그램을 이용하여 분석된 염기서열과 NCBI에 등록된 각각 어류의 유전자 염기서열을 비교하였다. 분석 결과 염기서열 상동성(identity)이 97% 이상인 종을 원재료 종으로 판별하였다. DNA barcode 시험법을 이용해 시중 유통되는 참돔, 돌돔, 황돔, 감성돔, 나일틸라피아, 옥돔, 옥두 수산가공품 46건에 대해 조사한 결과 위변조 사례는 나타나지 않았다. 그러나 시장에서 통용되는 일반명칭과 식품공전에 기술된 표준명칭이 상이하여 소비자의 혼란을 야기할 수 있어 수산물 가공식품 표기에 일반명칭과 더불어 표준명칭 또는 학명을 같이 기술하여야 할 것으로 판단되었다. DNA barcode sequences of commercial seafood products, which are difficult to morphologically discriminate, were analyzed to determine cases of food fraud. The gene sequences were analyzed by amplifying the COX I (cytochrome C oxidase subunit I) gene region of mitochondrial DNA, which is mainly used for species identification. The DNA barcode sequences were compared with the gene sequence of each fish registered in the US National Center for Biotechnology. A total of 46 processed seafood products (12 Pagrus majo, 4 Oplegnathus fasciatus, 7 Dentex tumifrons, 2 Acanthopagrus schlegelii, 7 Oreochromis niloticus, 6 Branchiostegus japonicus, 8 Branchiostegus albus) were investigated. Having DNA sequence identity of more than 97% was judged as the same species. As a result of this study, no cases of forgery and alteration were detected. However, some disparities in the commercial names used in local markets and the standard names given in the Korea Food Code were found, which may cause confusion for consumers. It is therefore suggested that the standard name or scientific name be displayed on seafood product labels.

식품과 축산물 HACCP의 차이점 분석 및 일원화 방안 도출

조아현(Ah-Hyeon Jo ),강주영(Ju-Yeong Kang),박은지(Eun-Ji Park),이한철(Han-Cheol Lee),이철수(Cheol-Soo Lee),김중범(Jung-Beom Kim) 한국식품과학회 2020 식품과학과 산업 Vol.53 No.1

Korea has been managed HACCP with food and livestock products separately, but it was incorporated into food and livestock product safety management certification standard in 2015. Currently, the notification is unified, but food sanitation act and the livestock products sanitary control act are not unified. These differences are leading to complaints from food and livestock corporation. In this review, the food sanitation act, enforcement regulations of the food sanitation act, the livestock products sanitary control act and enforcement regulations of the livestock products sanitary control act were compared and analyzed to identify the part which requires unification. As a result of the survey, the thirteen clauses were proposed to unify in the food sanitation act and the livestock products sanitary control act. The nineteen clauses were proposed to unify in enforcement regulations of the food sanitation act and enforcement regulations of the livestock products sanitary control act.

원전 비상디젤발전기 신뢰도 관리시스템 개발

송재주(Song jae ju),이정일(Lee jeong il),신진호(Sin jin ho),강주영(Kang joo yeong),이봉재(Yi bong jae) 한국정보과학회 2005 한국정보과학회 학술발표논문집 Vol.32 No.1

본 논문은 원자력발전소 비상디젤발전기의 신뢰도 및 성능변화 추이를 감시하고, 이에 필요한 데이터를 관리하는 비상디젤발전기 신뢰도 프로그램 개발에 관하여 기술하였다. 이 프로그램은 인트라넷 기술을 이용하여 신뢰도 감시, 성능 감시, 그리고 관련정보로 크게 3가지 모듈로 구분하여 개발하였다. 현재 이를 통하여 원전 비상디젤발전기의 운전 및 정기점검 데이터를 입력 관리할 수 있도록 서비스를 개시하였으며, 이미 기존에 입력된 데이터를 이용하여 신뢰도 및 이용 불능도를 계산할 수 있고 또한 성능변화 추이를 감시할 수 있다.

스마트 HACCP 효과 분석 모델 개발

이한철(Han-Cheol Lee),강주영(Ju-Yeong Kang),박은지(Eun-Ji Park),박민지(Min-Ji Park),오도경(Do-Gyung Oh),김찬영(Chan-Yeong Kim),정은선(Eun-Sun Jeong),김재명(Jai-Moung Kim),안영순(Yeong-Soon Ahn),김중범(Jung-Beom Kim) 한국식품과학회 2021 식품과학과 산업 Vol.54 No.3

Smart HACCP is a system that can check the monitoring of critical control point (CCP) in real time to implement improvement measures immediately after departure from limit criteria and prevent falsification of data by digitizing handwritten records. In this study, we developed the analysis model for the effectiveness of smart HACCP to compare and analyze with existing HACCP. By introducing of smart HACCP system, the evaluation index value of HACCP effectiveness for HACCP-certificated companies on a small scale increased by 9.25 points, corresponding to 11.52% of increase rate. General HACCP-certificated companies showed 4.52 point and 5.00% of increase rate by introducing of smart HACCP system. Thus, it was confirmed that the introduction of smart HACCP system contributes to the improvement of food safety management and especially it would be more effective for HACCP-certificated companies on a small scale than general HACCP-certificated companies.

생물전환으로 생리활성물질인 trifolin의 생합성

노혜령 ( Hye-ryeong Noh ),강주영 ( Ju-yeong Kang ),김봉규 ( Bong-gyu Kim ) 한국응용생명화학회 2021 Journal of Applied Biological Chemistry (J. Appl. Vol.64 No.3

Trifolin (kaempferol 3-O-galactoside)는 플라보놀 그룹에 속하는 물질로 아토피, 항균, 폐암에 효과가 있는 것으로 알려져 있다. Trifolin은 다양한 식물에서 추출하여 사용하고 있지만 추출 과정이 복잡하고, 수율이 낮으며, 추출을 위한 바이오매스를 얻는데 계절적 어려움이 있다. 생물전환은 저렴한 화합물에서 고부가가치 화학물질을 생산할 수 있는 대체 수단으로 이용된다. 본 연구에서는 naringenin으로부터 trifolin을 생합성하기 위해 3개의 유전자(PeFLS 및 OsUGE-PhUGT)를 각각의 대장균에 도입한 BL-FLS균주와 BL-UGTE균주를 이용하여 공조배양시스템을 개발하였다. Naringenin으로부터 trifolin을 생합성하기 위해 세포의 밀도, 생물전환 온도, 재조합 단백질 유도의 적정 IPTG농도 및 시간, 기질 공급 농도 등의 최적화를 실시하였다. 최적화된 공동 배양 발효 시스템을 통해 67.3 mg/L의 trifolin을 성공적으로 생합성 하였다. Kaempferol 3-O-galactoside (Trifolin), a member of the flavonol group, has been reported to have anticancer effects against promyelocytic leukemia, histocytic lymphoma, skin melanoma and lung cancer. Trifolin has been extracted and used from several plants, but the extraction process is complicated and the final yield is low. Biotransformation is an alternative tool to produce high value-added chemicals from inexpensive compounds. To synthesis trifolin from naringenin, three genes (PeFLS and OsUGE-PhUGT) were introduced into Escherichia coli, respectively. In order to synthesis trifolin from naringenin, a coculture fermentation system was established by optimizing the cell concentration, biotransformation temperature and medium, isopropyl-β-D-thiogalactoside (IPTG) concentration, substrate supply concentration, and recombinant protein induction time. The established optimal conditions for trifolin production were a 3:1 ratio of BL-UGTE to BL-FLS, induction of recombinant protein at 25 ℃ for 4 h after addition of 2.0 mM IPTG, biotransformation at 30 ℃, and supply of 300 μM naringenin. Through the optimized co-culture fermentation system, trifolin was biosynthesized up to 67.3 mg/L.

<특집>한국어 교육의 오늘 : 한글 자모 교육의 표준화 방안

원순옥 ( Sun Ok Won ),강주영 ( Ju Yeong Kang ) 한국민족연구원 2014 민족연구 Vol.0 No.59

This paper compares and analyzes Korean alphabet education performed variously now to grope ways to integrate Korean alphabet educations. For this, Korean alphabet systems regulated in ‘The rules of Korean orthography’ and ‘The standard pronunciation of Korean’ are examined, and ways to present Korean alphabet that appear in the textbooks used in Korean education for the foreigners and the textbooks used in the official language education at elementary schools are compared. As a result of comparison between these regulations and the textbooks, it seems desirous to implement systematic and effective Korean alphabet education through standardization such as contents, orders and methods provided in Korean alphabet education. The following matters shall be considered to standardize the Korean alphabet education. First, criteria over the number of consonants-vowels and the order of suggestions shall be established. Second, it shall be established whether to suggest the names of consonants-vowels and in which way and which stage they shall be suggested. Third, it shall be established whether the pronunciation would be marked and how the marking would be like. The Korean alphabet whoever use it, regardless of Koreans or foreigners is same. Therefore, the contents, orders, and methodologies suggested while they study Korean must be the same. Of course, the teaching ways depending on learners` variables can be different. However, basically, the directions of education which become a premise should be standardized. It will be helpful to make learners and instructors find suitable teaching methods and reduce confusion. To these ends, in the body, the plans to standardize Korean Alphabet Education are suggested as follows. The number of alphabet should be suggested with 40 letters. The order to suggest alphabet should follow the principles of making words in Hunmin Jeongeum to help recognize and remember the letters. The Korean names are better to be marked while educating Alphabet. The phonetic values of Alphabet should be suggested by IPA (International Phonetic Alphabet). If necessary, mother tongues of learners can be used.

PID제어를 통한 캔위성 낙하산 제어 및 안정적 착륙시스템의 구현

문승환(Seung Hwan Moon),강주영(Ju Yeong Kang),최준혁(Jun Hyeok Choi) 한국항공우주학회 2023 한국항공우주학회 학술발표회 논문집 Vol.2023 No.11

당침 당이 매실 청 품질에 미치는 영향

문광호,이한철,조아현,이서현,김나예슬,박은지,강주영,김중범,Mun, Kwang-Ho,Lee, Han-Cheol,Jo, Ah-Hyeon,Lee, Seo-Hyun,Kim, Na-Ye-Seul,Park, Eun-Ji,Kang, Ju-Yeong,Kim, Jung-Beom 한국식품영양학회 2019 韓國食品營養學會誌 Vol.32 No.3

The objective of this study was to evaluate the quality-based characteristics of Prunus mume fruit syrup, which is manufactured with various sugared sweeteners for suggestion of suitable alternative sweetener. Sweetener such as sucrose (MHP1), crystalline fructose (MHP2) and liquid fructo-oligosaccharide (MHP3) are used to manufacture Prunus mume fruit syrup. The sugar content of MHP1, MHP2 and MHP3 showed 53, 54 and $36^{\circ}Brix$, respectively. The total organic acid content of MHP1, MHP2 and MHP3 was 2.22, 3.07 and 3.71%. The total free sugar content of MHP1, MHP2 and MHP3 was 54.39, 47.52% and 31.62%, respectively. The appearance of MHP1 and MHP2 remained unchanged for the entire period but MHP3 had molded since the first week. This was as a result of the low total free sugar content in MHP3 sugared with liquid fructo-oligosaccharide compared to MHP1 and MHP2 sugared with solid sucrose and fructose. The sensory characteristics of MHP2 manufactured with crystalline fructose indicated an above average quality, indicating that it is difficult to manufacture Prunus mume fruit syrup using liquid sugar. It is suggested that crystalline fructose characterized solid form and lower glycemic index than sucrose be useful to manufacture Prunus mume fruit syrup as alternative sweetener.