모체로부터 태아세포의 발견 및 산전유전진단에의 이용

신종철 ( Jong Chul Shin ) 대한주산의학회 2000 Perinatology Vol.11 No.1

간세포 성장인자가 HT 세포주에서 matrix metalloproteinase 의 발현에 미치는 영향

신종철(Jong Chul Shin),문희봉(Hee Bong Moon),이지현(Jee Hyun Lee),양동은(Dong Eun Yang),이귀세라(Gui Se Ra Lee),이영(Young Lee),이종승(Jong Seong Lee),김창이(Chang Yi Kim),김수평(Soo Pyung Kim) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.12

N/A Objective : This study was performed to investigate the influence of hepatocyte growth factor (HGF) on matrix metalloproteinase (MMP), which are related in the lysis process of tissue during the invasion of trophoblasts. Method : HT cell line was treated with recombinant HGF (rHGF) of different concentration (0, 10, 50 and 100 ng/mL) and was cultured for 24 hours to check the changes in the expression of MMP-2 and MMP-9. Also, HT cell line was treated with recombinant HGF 50 ng/mL and was cultured for 24, 36, 48, and 72 hours to check the changes in the expression of MMPs according to the different time span. Total RNA were extracted from each cultured sample and RT-PCR and Western blotting were used to analyze the expression of MMP-2 and MMP-9. Results : MMP-2 mRNA expression with treated rHGF showed increase of 2, 2.5 and 2.2 times with the increase of concentration level of 10, 50 and 100 ng/mL accordingly, while MMP-2 protein expression were increased 1.4 and 1.5 times in 50 ng/mL and 100 ng/mL of rHGF respectively compared with that of normal control. MMP-9 mRNA showed no significant changes in its expression with all different levels of concentration, while MMP-9 protein showed 1.5 times increase with 10 ng/mL rHGF but 0.4 times decrease with 100 ng/mL. MMP-2 mRNA expression treated with recombinat HGF were increased 1.6 times with 24 hour culture and 2.3 times with 36 hour culture. MMP-2 protein showed 1.9 times increase only for the case of 24 hour culture. MMP-9 mRNA expression of recombinant HGF-treated groups was decreased 0.7 times compared with that of control group in 36 hours. MMP-9 protein expression were increased by 1.2, 1.6 and 1.9 times as culture time increase to 36, 48, and 72 hours accordingly, compared with that of normal control. Conclusion : This result suggests that the HGF might partially regulate the invasion of trophoblasts through MMP-2 and MMP-9.

간세포 성장인자가 HT 세포주에서 c - met 의 발현과 침투성에 미치는 영향

신종철(Jong Chul Shin),이지현(Jee Hyun Lee),양동은(Dong Eun Yang),오민정(Min Jeong Oh),문희봉(Hee Bong Moon),이영(Young Lee),김사진(Sa Jin Kim),이종승(Chong Seung Yi),김수평(Soo Pyung Kim) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.8

N/A Objective : To evaluate the invasive capacity of trophoblastic cells when treated with HGF, and see whether HGF induces c-met expression in vitro. Methods : HT cell line was treated with recombinant HGF (rHGF) at different concentrations (0, 10, 50 and 100 ng/mL) and cultured for 24 hours. To evaluate according different time of culture, HT cell line was pre-treated with 50 ng/mL rHGF and cultured for 24, 36, 48 and 72 hours. We analyzed the expression of c-met mRNA using reverse transcriptase-polymerase chain reaction and the expression of c-met protein using western blot in each samples. We also observed cellular invasion capacity through the invasion assay under a microscope and confirmed 72 kDa gelatinase and 92 kDa gelatinase expression patterns by zymography assay. Results : The expressions of c-met mRNA and protein were increased in all concentrations of rHGF, compared with that of normal control although it was not in dose-dependent fashion. In invasion assay, the number of invaded HT cells were increased in dose-dependent fashion, compared with that of normal control. In zymography ssay, the expression of 72 kDa gelatinase was increased in dose-dependent fashion, compared with the control. However, 92 kDa eglatinase was not detected in any studied group. Conclusion : These results suggests that HGF might be related to upregulation of trophoblast cell invasiveness by activation of c-met and subsequent induction of 72 kDa gelatinase.

코로나19 이후 사회적 변화에 따른 목회자의 정년을 어떻게 볼 것인가?

신종철 ( Shin Jong Cheol ) 개혁신학회 2022 개혁논총 Vol.59 No.-

코로나19는 한국 뿐만 아니라 전 세계를 완전히 바꾸어 놓았다. 토머스 프리드먼(Thomas Friedman)의 말대로 “세계는 이제 코로나 이전인 BC(Before Corona)와 코로나 이후인 AC(After Corona)로 구분될 것이다.”코로나는 한국 사회의 많은 부분에 영향을 주었다. 특히 코로나 19 상황이 지속되면서 ‘비정상적이라고 생각되던 것들을 자연스럽게 받아들여지는 결과’를 만들었다. 코로나 이전에 기독교인의 이미지는 ‘거리를 두고 싶은(32,2%),’ ‘이중적인(30.3%),’ ‘사기꾼 같은(29.1%)’ 등의 부정적인 이미지가 많았다. 코로나 이후는 현재 이런 부정적인 이미지가 더욱더 강해졌다. 이것은 코로나 이후에 한국교회의 회복과 부정적인 이미지 쇄신을 위해 리더쉽의 역활이 대단히 중요한 것임을 보여준다. 이런 맥락에서 목회자의 정년문제는 깊은 논의가 필요하다. 성경에 보면 하나님의 사명은 나이를 불문했다. 하나님의 말씀을 전하는 요건은 나이와는 상관이 없다는 것이 성경 전체를 통해서 보여주는 관점이다. 영적인 사역은 영적인 전쟁이다. 그런데 사탄은 끊임없이 사람을 배제하는 선택을 하도록 유도 한다. 이것은 오랜 목회의 경험과 리더십이 뛰어난 노년의 목회자들을 배제하는 결과를 초래한다. 이것은 성경적인 관점이 아니다. 그러므로 필자는 정년에 관하여 다음과 같이 제안한다. 목회자 정년을 현행대로 만 70세로 하되, 미국 개혁교단에서 행하고 있는 바와 같이 노회가 개교회의 형편에 따라 목회자 정년을 만3년을 더할 수 있도록 허락해주는 것이다. 하지만 총회 총대와 노회에서의 공직 사역들은 만70세로 한정해야 할 것이며, 나머지는 3년간은 오로지 교회 목양 사역(목회)에만 전념토록 정하는 것이다. 이 3년간의 기간은 예수님의 3년간의 공생애를 통해 보여준 바와 같이 가르치고, 전파하고 치유하는 일에 최선을 다해야 할 것이다. COVID-19 has completely changed not only Korea, but the whole world. Before the corona virus, Christians had much negative impression, such as ‘to be kept a distance from’ (32.2%), ‘double-minded’ (30.3%), and ‘like a con artist’ (29.1%). After Corona, negative impression on Christians has gotten deeper now. In this situation a powerful and influential spiritual leadership is required in order to change Korean church’s overall impression from negative to positive and eventually to fulfill the restoration of the Korean Church. In this context, we should scrutinize the regulation of the Pastor’s mandatory retirement age limit. According to the Bible, God’s mission is given to anyone regardless of his or her age. Therefore, this researcher propose the following regarding to the pastors’ mandatory retirement regulation. We may keep the mandatory retirement age limit as “70 years old” as it is now. However, I suggest that local Presbyteries could permit pastors the extension up to three years, depending on the circumstances of the local church, just as Reformed Church in America does. However, the extension should be applied to local church pastoral ministry only. Public services as a commissioner for presbytery or for the general assembly should be limited to pastors under the age of 71. Pastors who are granted with three-year extension should devote themselves to teaching, preaching, and healing, as Jesus did during his three-year public life.

電子商去來에 있어서 消費者保護에 관한 硏究

申鍾澈,高炯錫 한국해양대학교 사회과학연구소 2000 韓國海洋大學校 人文社會科學論叢 Vol.12 No.1

The Electronic Commerce that is rapidly developing in the trade system is being studied in various aspects. This paper investigates laws of International organizations and other nations about damages in relations to supply of goods or service out of various consummer's damages that taken place in the Electronic Commerce. Then it inquiries consummer's rights into the existing laws and systems, proposes the complementary measures at the same time.

<맘마미아>를 통한 영화와 뮤지컬의 안무 비교 분석 연구

신종철 한국무용학회 2019 한국무용학회지 Vol.19 No.1

본 연구의 목적은 하나의 콘텐츠를 이용한 영화와 뮤지컬 속에 등장하는 안무를 비교분석하여 어떠한 차이점을 보이는지 알아보는데 있다. 본 연구에서는 작품 <맘마미아>를 선정하여 영화영상과 뮤지컬공연실황을 토대로 안무가 나오는 뮤지컬영화 No. 7곡과 뮤지컬 No. 12곡을 중심으로 연구하였다. 그 결과 영화와 뮤지컬이 가지 는 특성을 이해하고 안무를 해야 한다는 결론에 도달 할 수 있었다. 첫째, 뮤지컬에서는 판타지 드라마 요소를 적극 활용한 안무가 극의 흐름을 도와준다. 둘째, 뮤지컬에서는 쇼 스톱퍼의 역할로 현장성을 강조한다. 셋째, 영화에서는 줄거리를 통하여 표현하고 뮤지컬에서는 장면을 통하여 표현한다. 넷째, 영화는 화면구성기법으로 표현하며 뮤지컬은 공간기법으로 표현한다. 장르전환이 되고 있는 <맘마미아>작품처럼 우리나라의 많은 창작품 들 또한 다양한 문화콘텐츠를 통하여 2차, 3차로 그 영역이 확대되는 것을 연구하는 것이 무엇보다 중요하다. The objective of this study is to understand differences by comparatively analyzing the choreography shown in a film and musical using the same contents. After selecting a work <Mamma Mia>, this study mainly researched the No.7 music of the musical film and the No.12 music of the musical with choreography based on the film images and the actual musical performance. In the results, the different presentation methods between film and musical had lots of effects on the choreography. First, in the musical, the elements of fantasy drama are actively used to help the flow of the drama. Second, in the musical, the sense of realism is emphasized as a role of show stopper. Third, it is presented through plot in the film while it is presented through scenes in the musical. Fourth, the film is presented through screen techniques while the musical is presented through space techniques. It is important to research the expansion of many Korean creations to the 2nd and 3rd areas through diverse cultural contents just like <Mamma Mia> whose genre has been successfully changed to another one.

반응성 염료의 색도 제거를 위한 균주 분리 및 최적화

신종철(Jong Chul Shin),최광근(Kwang Keun Choi),전현희(Hyun Hee Jeon),김상용(Sang Young Kim),이진원(Jinwon Lee) 한국생물공학회 2004 KSBB Journal Vol.19 No.3

Genetics in Human Medicine

신종철 가톨릭중앙의료원 가톨릭암센터 1996 암심포지움 Vol.- No.2

假想空間에서의 個人情報保護에 관한 小考

申鍾澈,高炯錫 한국해양대학교 국제해양연구소 2001 韓國海洋大學校 人文社會科學論叢 Vol.13 No.1

Abstract In the cyber-space contrary to the real world, an invasion of personal data occurs frequently. Therefore, International organizations and the countries of the world are protecting personal data in the caber-space by various legislations, and our country also is making legislative and systemic efforts. In this thesis, I examined our country's regulations and contents of system about protection of personal data and I suggested problem, supplementation for the problems and precaution against the damage.

集合動産 讓渡擔保의 法理構成에 관한 比較法的 考察

申鍾澈,金仁猷 韓國海洋大學校 人文社會科學大學 1996 韓國海洋大學校 人文社會科學論叢 Vol.- No.4

Die Sicherungstibereignung fur Gesamtmobiliar ist nicht Vorsherift darum. und in dieser Hinsicht ist Gesamtmobiliar in ein Ganzes vereinigen Sicherhei, Scherungsubereignung fur Gesamtmobiliar hat viel zu losen gesetliche Problems, welches nur auf allgemeine Sicherungsubereignung Theorie konnen nicht osen. Diesc Dissertation war die Forshcung uber folgendermaβen Problems versucht. Wieich die Sicherungsubereignung fur Gesamtmobiliar Rechtsaufbauen konstituieren kann, Unter Wolchen Voraussetzung ist Gensamtmobiliar sich selbstandig Objekt fur Sicherungsubereignung, d.h. Problem uber Bercich Bestimmitheit des Objekts, und Problem uber Pulizitatsmittel, Problem zur Wirkung uber Sicherungsbereignung fur Gesamtmobiliar, usw. Hcutiegentags, Mit wirtschaftlich Entwicklung, Sicheungsubereignung fur Gesamtmobiliar ist in wirklich Handlung benutzt. Wenn Sache sich versammelt, objektiv einzig wirtschaftlich Wert hat, der ganze Kram in Geschaftsvcrbindung handelt, so handelt als gesetzlich ein Sache, Folglich, dic Gesamtsachen Theorie ist richtig im Rcchtsaufbaucn uer Sicheungsubereignung fur Gesamtmobiliar.