임신성 당뇨에 대한 50-g, 1시간 Glucose Screening 검사

이종승 ( Chong Seong Yi ),김도강 ( Dou Kang Kim ),신종철 ( Jong Chul Shin ),김사진 ( Sa Jin Kim ),홍승덕 ( Seoung Dug Hong ),이종건 ( Jong Kun Lee ),나종구 ( Jong Gu Rha ),김창이 ( Chang Yee Kim ),김수평 ( Soo Pyung Kim ) 대한주산의학회 1994 大韓周産醫學會雜誌 Vol.5 No.4

간세포 성장인자가 HT 세포주에서 matrix metalloproteinase 의 발현에 미치는 영향

신종철(Jong Chul Shin),문희봉(Hee Bong Moon),이지현(Jee Hyun Lee),양동은(Dong Eun Yang),이귀세라(Gui Se Ra Lee),이영(Young Lee),이종승(Jong Seong Lee),김창이(Chang Yi Kim),김수평(Soo Pyung Kim) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.12

N/A Objective : This study was performed to investigate the influence of hepatocyte growth factor (HGF) on matrix metalloproteinase (MMP), which are related in the lysis process of tissue during the invasion of trophoblasts. Method : HT cell line was treated with recombinant HGF (rHGF) of different concentration (0, 10, 50 and 100 ng/mL) and was cultured for 24 hours to check the changes in the expression of MMP-2 and MMP-9. Also, HT cell line was treated with recombinant HGF 50 ng/mL and was cultured for 24, 36, 48, and 72 hours to check the changes in the expression of MMPs according to the different time span. Total RNA were extracted from each cultured sample and RT-PCR and Western blotting were used to analyze the expression of MMP-2 and MMP-9. Results : MMP-2 mRNA expression with treated rHGF showed increase of 2, 2.5 and 2.2 times with the increase of concentration level of 10, 50 and 100 ng/mL accordingly, while MMP-2 protein expression were increased 1.4 and 1.5 times in 50 ng/mL and 100 ng/mL of rHGF respectively compared with that of normal control. MMP-9 mRNA showed no significant changes in its expression with all different levels of concentration, while MMP-9 protein showed 1.5 times increase with 10 ng/mL rHGF but 0.4 times decrease with 100 ng/mL. MMP-2 mRNA expression treated with recombinat HGF were increased 1.6 times with 24 hour culture and 2.3 times with 36 hour culture. MMP-2 protein showed 1.9 times increase only for the case of 24 hour culture. MMP-9 mRNA expression of recombinant HGF-treated groups was decreased 0.7 times compared with that of control group in 36 hours. MMP-9 protein expression were increased by 1.2, 1.6 and 1.9 times as culture time increase to 36, 48, and 72 hours accordingly, compared with that of normal control. Conclusion : This result suggests that the HGF might partially regulate the invasion of trophoblasts through MMP-2 and MMP-9.

새로 발생한 13 q32.2 → qter 결손과 동반된 13 번 환상염색체 환아 1 예

황성진(Seong Jin Hwang),이지현(Jee Hyun Lee),박인양(In Yang Park),문희봉(Hee Bong Moon),오준환(Joon Hwan Oh),이귀세라(Gui Se Ra Lee),이종승(Chong Seung Yi),신종철(Jong Chul Shin),김수평(Soo Pyung Kim) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.2

We report a case of ring chromosome 13 with a distal deletion of 13q32.2→qter observed in a fetus who was referred to our institution at term due to severe growth restriction and multiple congenital malformations on ultrasonographic examination. This boy was born by vaginal delivery at 39 weeks in gestation. His weight, head circumference and height were less than the 3 percentile of gestational age. Apgar score was 7 at 1 minute and 9 at 5 minutes. He showed microcephaly, large forehead, low set ears, hypertelorism, flat nasal bridge, and micrognathia. The genitalia was ambiguous, showing severe hypoplasia of the penis. The anus was ectopic, displaced anteriorly from its normal position but with a normal opening and function. Neurologic examination was normal. Echocardiogram done at 2 weeks of life showed a persistent foramen ovale and a ventriculoseptal defect (type II) with increased pulmonary hypertension. MRI examination of the brain showed poorly demarcating corpus callosum suspecting agenesis of corpus callosum. Also, cerebellar vermis was small and hypoplastic, mimicking a variant form of Dandy-Walker malformation. MRI of the pelvis showed a tubular structure in pelvic cavity, suspicious of uterine remnant, between urinary bladder and rectum, and a inguinal hernia was noted in the left side. In the abdominal cavity enlarged adrenal glands were noted, and hormonal study showed elevated 17-α-OH-progesterone (168.9 ng/ml) with normal 17-KS and 17-OHCS levels. Gastrointestinal and urogenital system were otherwise normal. Cytogenetic analysis of the parents were both normal but the newborn showed 46, XY, r(13), de novo, with deletion points q32.2→qter. Our findings are in line with previous reports about chromosome 13 deletions, in which loss of the critical point leads to major malformations like brain anomalies and ambiguous genitalia.

경증 주산기 이환율의 감소를 위한 적절한 분만 주수

박인양(In Yang Park),이종승(Chong Seong Yi),신종철(Jong Chul Shin),이지현(Ji Hyun Lee),이현정(Hyun Jeong Lee),강대호(Dae Ho Kang),김사진(Sa Jin Kim),김수평(Soo Pyung Kim) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.3

N/A Objective: Much emphasis has been placed on the morbidity and mortality of infants delivered before 32 weeks of gestation, including intraventricular hemorrhage and respiratory distress. The incidence of these complications and their association with long-term sequelae are well defined. This information is important, especially when decisions regarding delivery have to be made. Although delivery at >32 weeks of gestation may be considered free of serious sequelae of prematurity, morbidities are still associated with delivery between 32 and 36 weeks of gestation. The purpose of this study is to determine the incidence of minor morbidities associated with premature delivery between 32 and 36 weeks of gestation. We tried to find out the proper time to decrease the minor perinatal morbidities and the adverse effect of tocolytic treatment. Met hod: The study population is consisted of infants delivered between 20 and 36 weeks of gestation at Kang Nam St. Mary's hospital from 1995 to 1999. Maternal and neonatal charts were abstracted for maternal past history, pregnancy complications and neonatal demographics comparing complications present at each gestational week. Mann-Whitney test and χ2 test were used to assess statistical significance. Results: There was no significant difference of delivery time due to maternal age and parity. There was increased risk of low Apgar score and low birth weight before 34 weeks of gestation. Neonatal death was significantly high before 32 weeks of gestation. Neonatal death, sepsis, intraventricular hemorrhage, respiratory distress, ventilatory equipment use was significantly high before 32-33 weeks of gestation. Hypothermia, feeding difficulty, jaundice, NICU admission was significantly high before 30, 32, 35, 35 weeks of gestation. So it is approved that minor perinatal morbidity was decreased after 34-35 weeks of gestation. Conclusion: Major morbidity was significantly high before 32-33 weeks of gestation and Minor morbidity was significantly high before 34 weeks of gestation. Therefore considering of minor and major morbidity, it is reasonable to postpone the preterm delivery until 34 weeks of gestation.

토끼 태아에서 표피성장인자 투여가 코티졸 , 티록신 , 프로락틴의 생성에 미치는 영향

김수평(Soo Pyung Kim),신종철(Jong Chul Shin),이종건(Jong Kun Lee),김용욱(Yong Wook Kim),변태섭(Tae Sup Byun),신재인(Jae In Shin),이종승(Chong Seong Yi),나종구(Jong Gu Rha) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.12

N/A Respiratory distress syndrome (RDS) is a major cause of death in premature neonates, and it is caused by the failure of morphological and biochemical lung maturation (synthesis and secretion of lung surfactant). It is known that cortisol, thyroxine, prolactin, epidermal growth factor (EGF), and estrogen accelerate the lung maturation. Cortisol and thyroxine are currently used in the antenatal treatment for the prevention of RDS in premature neonates. In order to evaluate the effect of EGF on the levels of cortsol, thyroxine, and prolactin, this study was undertaken. Phosphate buffered saline (PBS) with and without EGF was directly injected into the 25 days gestational fetus in uterus. Blood was collected for the measurement of cortisol, thyroxine, and prolactin one day or two days after the injection. Body weights and lung weights were also measured. The results were as follows: 1. There was no significant difference in body weights and lung weights between PBS-treated group(control group) and EGF-treated poup(experimental group), 24 hours and 48 hours after the injection. 2. 24 hours after the injection, the levels of cortisol were significantly inaeased in the EGF-treated group compared with those in the PBS-treated group. However 48 hours after the injection, there was no significant difference in the levels of cortisol between the two groups. The levels of thyroxine and prolactin in the EGF-treated group did not significantly differ from those in the PBS-treated group 24 hours and 48 hours after the injection. In conclusion, in vivo, the synthesis of cortisol may be affected by EGF treatment, which suggests that the action of EGF for lung maturation may be partially mediated by the increased endogenous levels of cortisol.

전신성 홍반성낭창 환자의 임신결과에 관한 임상적 연구

노승혜 ( Seung Hye Rho ),백은정 ( Eun Jeong Baik ),이종승 ( Jong Seong Yi ),김사진 ( Sa Jin Kim ),박종섭 ( Jong Sup Park ),신종철 ( Jong Chul Shin ),이종건 ( Jong Kun Lee ),김수평 ( Soo Pyung Kim ) 대한주산의학회 1995 大韓周産醫學會雜誌 Vol.6 No.4

정상 임신부의 Tumor necrosis factor - a (TNF - a) , Vascular cell adhesion molecule - 1 (VCAM - 1) 및 지질과산화의 혈장내 농도

정대영,신종철,이귀세라,김은중,이종승,이초희,김사진,김수평,진홍기,권인 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.3

연구목적: 이 연구의 목적은 정상 임신부에서 임신 주수의 증가에 따라서 Tumor necrosis factor-α(TNF-α), Vascular cell adhesion molecule-1(VCAM-1) 및 lipid peroxide(지질과산화)의 혈장 내 농도의 변화를 알아보고자 하였다. 연구방법: 건강한 임신부와 임신의 경험이 없는 비임신 여성을 대상으로 하였다. 임신부는 임신 제 1분기(임신 4∼14주), 임신 제 2분기(임신 15∼29주), 임신 제 3분기(임신 30∼41주)로 나누어서 각 해당되는 주수의 임신부로부터 혈액을 채취하여 혈장을 분리한 뒤 TNF-α와 VCAM-1을 immunoassay에 의하여, 지질산화는 colorimetric assay에 의하여 측정하여 통계 분석하였다. 결과 및 결론: 혈장 내 TNF-α는 비임신 여성에 비하여 임신 제 2, 3분기에 유의적인 증가를 보였으며, 혈장 내 VCAM-1의 농도는 비임신 여성에 비하여 임신 제 1분기에 유의적인 증가가 있었다. 지질과산화의 혈장 내 농도는 임신 전기간에 걸쳐서 비임신 여성과 유의적인 차이는 없었다. 결론: 임신 시 혈장 내 TNF-α, VCAM-1 및 지질과산화의 변화는 비임신 여성에 비하여 TNF-α의 경우 제 2, 3 분기에, VCAM-1의 경우 임신 제 1 분기에 증가하며 지질과산화는 임신에 의한 변화는 없었다. 본 연구는 앞으로 문제성 있는 임신의 예측과 중증의 정도를 예견하는 데 있어서 기초 연구가 될 수 있을 것으로 생각된다. Objective: The purpose of study was to assess in a longitudinal study of maternal plasma concentrations of Tumor necrosis factor-α(TNF-α), Vascular cell adhesion molecule-1(VCAM-1), Lipid peroxide (malonaldehyde, MDA) in uncomplicated pregnancy. Methods: Blood was collected from healthy women at 4 to 41 weeks' gestation and non-pregnant women. Plasma samples were measured by immunoassay for TNF-α, VACM-1 and by colorimetric assay for lipid peroxide, and data were statistically analyzed. Results: Plasma concentration of TNF-α was not significantly elevated during first trimester compared with non-pregnant women, but significantly elevated during second and third trimester compared with non-pregnant women. Plasma concentration of VCAM-1 was significantly elevated during first trimester compared with non-pregnant women. Plasma concentration of lipid peroxide was not significantly elevated during pregnancy compared with non-pregnant women. Conclusion: The plasma concentration of TNF-α and VCAM-1 were significantly higher than that of non-pregnant state during second and third trimester in case of TNF-α, and during first trimester in case of VCAM-1. But the plasma concentration of lipid peroxide during pregnancy was not significantly different from that of non-pregnant, and the plasma concentration was kept up constant levels during gestation. These were seems to be meant that abnormal pregnancy would be happened if the level is above or below the measured level.