Novel Nucleotide Variations, Haplotypes Structure and Associations with Growth Related Traits of Goat AT Motif-Binding Factor (ATBF1) Gene

Xiaoyan Zhang,Xianfeng Wu,Wenchao Jia,Chuanying Pan,Xiangcheng Li,Chuzhao Lei,Hong Chen,Xianyong Lan 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.10

The AT motif-binding factor (ATBF1) not only interacts with protein inhibitor of activated signal transducer and activator of transcription 3 (STAT3) (PIAS3) to suppress STAT3 signaling regulating embryo early development and cell differentiation, but is required for early activation of the pituitary specific transcription factor 1 (Pit1) gene (also known as POU1F1) critically affecting mammalian growth and development. The goal of this study was to detect novel nucleotide variations and haplotypes structure of the ATBF1 gene, as well as to test their associations with growth-related traits in goats. Herein, a total of seven novel single nucleotide polymorphisms (SNPs) (SNP 1-7) within this gene were found in two well-known Chinese native goat breeds. Haplotypes structure analysis demonstrated that there were four haplotypes in Hainan black goat while seventeen haplotypes in Xinong Saanen dairy goat, and both breeds only shared one haplotype (hap1). Association testing revealed that the SNP2, SNP5, SNP6, and SNP7 loci were also found to significantly associate with growth-related traits in goats, respectively. Moreover, one diplotype in Xinong Saanen dairy goats significantly linked to growth related traits. These preliminary findings not only would extend the spectrum of genetic variations of the goat ATBF1 gene, but also would contribute to implementing marker-assisted selection in genetics and breeding in goats.

Novel Nucleotide Variations, Haplotypes Structure and Associations with Growth Related Traits of Goat AT Motif-Binding Factor (ATBF1) Gene

Zhang, Xiaoyan,Wu, Xianfeng,Jia, Wenchao,Pan, Chuanying,Li, Xiangcheng,Lei, Chuzhao,Chen, Hong,Lan, Xianyong Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.10

The AT motif-binding factor (ATBF1) not only interacts with protein inhibitor of activated signal transducer and activator of transcription 3 (STAT3) (PIAS3) to suppress STAT3 signaling regulating embryo early development and cell differentiation, but is required for early activation of the pituitary specific transcription factor 1 (Pit1) gene (also known as POU1F1) critically affecting mammalian growth and development. The goal of this study was to detect novel nucleotide variations and haplotypes structure of the ATBF1 gene, as well as to test their associations with growth-related traits in goats. Herein, a total of seven novel single nucleotide polymorphisms (SNPs) (SNP 1-7) within this gene were found in two well-known Chinese native goat breeds. Haplotypes structure analysis demonstrated that there were four haplotypes in Hainan black goat while seventeen haplotypes in Xinong Saanen dairy goat, and both breeds only shared one haplotype (hap1). Association testing revealed that the SNP2, SNP5, SNP6, and SNP7 loci were also found to significantly associate with growth-related traits in goats, respectively. Moreover, one diplotype in Xinong Saanen dairy goats significantly linked to growth related traits. These preliminary findings not only would extend the spectrum of genetic variations of the goat ATBF1 gene, but also would contribute to implementing marker-assisted selection in genetics and breeding in goats.

Gut Microbiota Alteration Influences Colorectal Cancer Metastasis to the Liver by Remodeling the Liver Immune Microenvironment

Yuan Na,Li Xiaoyan,Wang Meng,Zhang Zhilin,Qiao Lu,Gao Yamei,Xu Xinjian,Zhi Jie,Li Yang,Li Zhongxin,Jia Yitao 거트앤리버 소화기연관학회협의회 2022 Gut and Liver Vol.16 No.4

Background/Aims:This study aimed to explore the effect of gut microbiota-regulated Kupffer cells (KCs) on colorectal cancer (CRC) liver metastasis. Methods: A series of in vivo and in vitro researches were showed to demonstrate the gut microbiota and its possible mechanism in CRC liver metastasis. Results: Fewer liver metastases were identified in the ampicillin-streptomycin-colistin and colistin groups. Increased proportions of Parabacteroides goldsteinii, Bacteroides vulgatus, Bacteroides thetaiotaomicron, and Bacteroides uniformis were observed in the colistin group. The significant expansion of KCs was identified in the ampicillin-streptomycin-colistin and colistin groups. B. vulgatus levels were positively correlated with KC levels. More liver metastases were observed in the vancomycin group. An increased abundance of Parabacteroides distasonis and Proteus mirabilis and an obvious reduction of KCs were noted in the vancomycin group. P. mirabilis levels were negatively related to KC levels. The number of liver metastatic nodules was increased in the P. mirabilis group and decreased in the B. vulgatus group. The number of KCs decreased in the P. mirabilis group and increased in the B. vulgatus group. In vitro, as P. mirabilis or B. vulgatus doses increased, there was an opposite effect on KC proliferation in dose- and time-dependent manners. P. mirabilis induced CT26 cell migration by controlling KC proliferation, whereas B. vulgatus prevented this migration. Conclusions: An increased abundance of P. mirabilis and decreased amount of B. vulgatus play key roles in CRC liver metastasis, which might be related to KC reductions in the liver.

Fire-Induced Failure of Long-Term Service and Fresh Concrete Beams Subjected to Fire

Meng Wang,Zhen Guo,Xiaoyan Jia,Yue Yin,Chuang Ma 대한토목학회 2021 KSCE Journal of Civil Engineering Vol.25 No.10

In engineering, reinforced concrete beams are subjected to natural erosion and the working stress state. However, previous studies regarding structural fire have focused on new concrete beams, and most results are obtained from no-stress-state tests. Hence, research that considers the natural erosion and working stress states of beam members in fire is lacking. In this study, fire experiments were performed to investigate the differences between long-term service concrete beams and new elements under loads. A technique using ARAMIS three-dimensional (3D) optical strain measurement was adopted; it provides increased sample throughput and 3D measurements that indicate the change in strain more intuitively. Results show that the moisture content of concrete determines the spalling degree of concrete under the influence of working stress. With the increase of concrete moisture content, the range of temperature of heated and backfire surface increases and the spalling phenomenon is more obvious, especially when the moisture content exceeds 2.5%. In addition, the long-term service specimens are affected by fire more easily, thereby accelerating the destruction process owing to the degree of carbonation.

Alfalfa xenomiR-162 targets G protein subunit gamma 11 to regulate milk protein synthesis in bovine mammary epithelial cells

Meng Guizhi,Duan Hongjuan,Jia Jingying,Liu Baobao,Ma Yun,Cai Xiaoyan 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.3

Objective: It was shown that microRNAs (miRNAs) play an important role in milk protein synthesis. However, the post-transcriptional regulation of casein expression by exogenous miRNA (xeno-miRNAs) in ruminants remains unclear. This study explores the regulatory roles of alfalfa xeno-miR162 on casein synthesis in bovine mammary epithelial cells (bMECs). Methods: The effects of alfalfa xenomiR-162 and G protein subunit gamma 11 (GNG11) on proliferation and milk protein metabolism of bMECs were detected by 5-Ethynyl-2′- Deoxyuridine (EdU) staining, flow cytometry, cell counting kit-8 (CCK-8), enzyme-linked immunosorbent assay, quantitative real-time polymerase chain reaction (qRT-PCR), and Western blot. Dual-luciferase reporter assay was used to verify the targeting relationship between GNG11 and xenomiR-162. Results: Results showed that over-expression of xenomiR-162 inhibited cell proliferation but promoted apoptosis, which also up-regulated the expression of several casein coding genes, including CSN1S1, CSN1S2, and CSN3, while decreasing the expression of CSN2. Furthermore, the targeting relationship between GNG11 and xenomiR-162 was determined, and it was confirmed that GNG11 silencing also inhibited cell proliferation but promoted apoptosis and reduced the expression of casein coding genes and genes related to the mammalian target of rapamycin (mTOR) pathway. Conclusion: Alfalfa xenomiR-162 appears to regulate bMECs proliferation and milk protein synthesis via GNG11 in the mTOR pathway, suggesting that this xeno-miRNA could be harnessed to modulate CSN3 expression in dairy cows, and increase κ-casein contents in milk.

Retraction: Inhibition of ClC-5 suppresses proliferation and induces apoptosis in cholangiocarcinoma cells through the Wnt/β-catenin signaling pathway

Zhe Shi,Liyuan Zhou,Yan Zhou,Xiaoyan Jia,Xiangjun Yu,Xiaohong An,Yanzhen Han Korean Society for Biochemistry and Molecular Biol 2024 BMB Reports Vol.57 No.2

Novel modulation method for torque ripple suppression of brushless DC motors based on SIMO DC-DC converter

Sun, Shikai,Guo, Hui,Zhang, Yimeng,Jia, Yupeng,Lv, Hongliang,Song, Qingwen,Tang, Xiaoyan,Zhang, Yuming The Korean Institute of Power Electronics 2020 JOURNAL OF POWER ELECTRONICS Vol.20 No.3

In this paper, a novel modulation method is proposed for the torque ripple suppression of a brushless DC motor (BLDCM) during both the commutation period and the speed regulation period. The main sources of torque ripple are the imbalance current during commutation and the PWM during speed regulation. A single-inductor multiple-output (SIMO) Buck DC-DC converter is adopted to generate input voltage for the inverter driver of a BLDCM. During the commutation period, the input voltage of an inverter can be adjusted to four times the back electromotive force (EMF), which suppresses the torque ripple caused by imbalance commutation current. Meanwhile, by changing the output voltage of a SIMO DC-DC, the speed of a BLDCM can be regulated without PWM on the inverter switches, which also suppresses the torque ripple. A control algorithm is applied on a Field Programmable Gate Array (FPGA), and the proposed BLDCM drive prototype is fabricated and tested. Measurement results show that with the proposed speed regulation strategy, the torque ripple is suppressed from 33.3% to 20.1%, and that with the proposed suppression strategy in commutation, the torque ripple is suppressed further to 5.3%.

Inhibition of ClC-5 suppresses proliferation and induces apoptosis in cholangiocarcinoma cells through the Wnt/β-catenin signaling pathway

( Zhe Shi ),( Liyuan Zhou ),( Yan Zhou ),( Xiaoyan Jia ),( Xiangjun Yu ),( Xiaohong An ),( Yanzhen Han ) 생화학분자생물학회 2022 BMB Reports Vol.55 No.6

Chloride channel-5 (ClC-5), an important branch of the ClC family, is involved in the regulation of the proliferation and cell-fate of a variety of cells, including tumor cells. However, its function in cholangiocarcinoma (CCA) cells remains enigmatic. Here, we discovered that ClC-5 was up-regulated in CCA tissues and CCA cell lines, while ClC-5 silencing inhibited CCA cell proliferation and induced apoptosis. Further mechanism studies revealed that ClC-5 inhibition could inhibit Wnt/β-catenin signaling activity and further activate the mitochondria apoptotic pathway in CCA cells. Furthermore, rescuing Wnt/β-catenin signaling activation eliminated the anti-tumor function of ClC-5 knockdown. Together, our research findings illustrated that ClC-5 inhibition plays an anti-tumor role in CCA cells via inhibiting the activity of the Wnt/β-catenin pathway, which in turn activates the mitochondrial apoptotic pathway. [BMB Reports 2022; 55(6): 299-304]

Cyclophosphamide-induced HCN1 channel upregulation in interstitial Cajal-like cells leads to bladder hyperactivity in mice

Qian Liu,Zhou Long,Xingyou Dong,Teng Zhang,Jiang Zhao,Bishao Sun,Jingzhen Zhu,Jia Li,Qingqing Wang,Zhenxing Yang,Xiaoyan Hu,Longkun Li 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are confirmed to be expressed in bladder interstitial Cajal-like cells (ICC-LCs), but little is known about their possible role in cystitis-associated bladder dysfunction. The present study aimed to determine the functional role of HCN channels in regulating bladder function under inflammatory conditions. Sixty female wild-type C57BL/6J mice and sixty female HCN1-knockout mice were randomly assigned to experimental and control groups, respectively. Cyclophosphamide (CYP)-induced cystitis models were successfully established in these mice. CYP treatment significantly enhanced HCN channel protein expression and Ih density and significantly altered bladder HCN1 channel regulatory proteins. Carbachol (CCH) and forskolin (FSK) exerted significant effects on bladder ICC-LC [Ca2+]i in CYP-treated wild-type (WT) mice, and HCN1 channel ablation significantly decreased the effects of CCH and FSK on bladder ICC-LC [Ca2+]i in both naive and CYP-treated mice. CYP treatment significantly potentiated the spontaneous contractions and CCH (0.001–10 μM)-induced phasic contractions of detrusor strips, and HCN1 channel deletion significantly abated such effects. Finally, we demonstrated that the development of CYP-induced bladder overactivity was reversed in HCN1 / mice. Taken together, our results suggest that CYP-induced enhancements of HCN1 channel expression and function in bladder ICC-LCs are essential for cystitis-associated bladder hyperactivity development, indicating that the HCN1 channel may be a novel therapeutic target for managing bladder hyperactivity.

Global Output Feedback Stabilization Control for Nonlinear Systems without Backstepping

Xiao Yan,Hongzheng Quan,Fujin Jia 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.6

In this paper, we study the global output feedback stabilization (GOFS) of nonlinear systems with unknown functions (UFs). Firstly, a lemma is introduced to solve UFs, which avoids the shortcomings of the approximate algorithms. At the same time, this lemma avoids the assumptions of UFs, thus reducing the conservatism of the algorithms. Secondly, based on Lyapunov function analysis, the controller is designed, which solves the GOFS problem of the controlled system. This control algorithm adopts nonbackstepping, so it avoids the complicated calculation of backstepping. Finally, the control algorithm is applied to the Duffing system to verify its effectiveness.