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UV 및 젖산 처리에 의한 난각 표면의 Salmonella 억제 효과
손원근,이철현,강호조 한국수의공중보건학회 2002 예방수의학회지 Vol.26 No.4
The inhibitory effects were investigated of 254-nm ultraviolet (UV) radiation and lactic acid (LA) treatment on populations of Salmonella (S) enteritidis on eggshell surface. All UV exposures (730, 1,580, 3,000 W/cm²) for 30 sec significantly reduced S enteritidis (P 0.05). UV irradiation with 3,000 μW/cm² almost completely eliminated S enteritidis on inoculated eggshells by 99%. These results suggest that the higher intensity of UV irradiation may be associated with the higher germicidal activity. However, no significant differences were detected between UV exposure times. The treatments of LA at the concentration of 0.1, 0.3 and 0.5 % also specifically decreased the bacterial cell populations (0.91 1.14 log CFU) on the eggshell compared with the control (6.92 log CFU). The treatment of LA at the concentration of 0.5 % had the reaction of a 2.52 log unit storage for 9 days. The combined treatment of UV (3,000 μW/cm²) and LA(0.5%) decreased S enteritidis populations on eggshell by 3.85 log unit, compared wth the control (P 0.05), and its inhibitory effect was stronger with storage time.
마우스에서 listeria monocytogenes의 장관정착과 그 결정요인 III. 장관정착에 미치는 요인시험
손원근(Won Geun Son),강호조(Ho Jo Kang) 한국예방수의학회 1995 예방수의학회지 Vol.19 No.2
In SPF-ICR mice inoculated orally with Listeria monocytogenes investigated were effect of gastric juice, neutralizer of gastric acid, hemolysin and p60 for bacterial colonization. In in vitro stomach contents of heat-treated, HCI-treated to pH<3.0, and normal contents as control, bacterial counts in heat-treated samples were similar to those in control group 8 hrs after inoculation. Bacterial growth was inhibited markedly In HCI-treated samples at 2 hrs after inoculation. Bacterial counts respectively of 3.16 log CFU /g and 2.66 log CFU /g in stomach of mice treated with sodium bicarbonate and ranitidine-HCI were prominent than those in control group(1.0 log CFU /g) at 2 hrs after inoculation. Moreover, the cecal colonization levels in sodium bicarbonate-treated group(5.57 log CFU /g) or ranitidine-HCI-treated group(5.13 log CFU /g) were higher than those of control group(2.27 log CFU /g). The cecal colonization level by L. monocytogenes strain Mackaness harvouring virulent factors as hemolysin and p60 was higher than those by such strains deficient for virulent factors as RI (hemolysin⁺p60⁻) or M3(hemolysin⁻ p60⁺).
Escherichia coli O157:H7 Intimin의 Expression 및 C-terminal 부위의 특성
손원근,Gannon, V. P. J. 한국수의공중보건학회 2001 예방수의학회지 Vol.25 No.3
Portions of the intimin genes of Escherichia coli O157:H7 strain 319 and of the enteropathogenic E. coli O127:H6 strain E2348/69 were amplified by PCR and cloned into pET-28a (+) expression vectors. The entire 934 aa of E. coli O157:H7 intimin, the C-terminal 306 aa of E. coli O157:H7 intimin, and the C-terminal 311 aa of E. coli O127:H6 intimin were expressed as protein fusions with a six histidine residue tag (His-tag) in pET-28a (+). Rabbit antisera raised against the six His-tag 3' end one-third portion of E. coli O157:H7 intimin protein fusion reacted strongly in Western blots with original antigen and its homology protein, the six His-tag-fusioned protein containing full-length intimin of E. coli O157:H7, while reacting weakly with the his-fusioned intimin from enteropathogenic E. coli serotype O127:H6. In contrast with the antiseraraised against the sis His-tag 3'end portion of E. coli O127:H6 recognized only the original antigen, His-intO127C.
Palatal obturator restoration of a patient with partial maxillectomy
( Geun-won Jang ),( Cha-hwan Bae ),( Mee-kyoung Son ),( Yu-ri Heo ) 조선대학교 구강생물학연구소 2016 Oral Biology Research (Oral Biol Res) Vol.40 No.3
Patients who undergo partial maxillectomy for cancer, mainly of the maxillary sinus or the palate, are vulnerable to passage of food or liquids into the sinus and nasal cavity due to the defect in the hard and soft palate. Also, these patients produce hypernasal sounds during speech because of air leakage into the nasal cavity. A palatal obturator is one of the most commonly used maxillo-facial prosthesis for rehabilitation of maxillary defects, and it separates the nasal cavity from the oral cavity, enabling mastication and deglutition, and it not only improves speech by preventing air leakage but also enhances facial esthetics. In this case report, a palatal obturator was applied to a patient who underwent partial maxillectomy for adenoid cystic carcinoma, and it showed favorable results in terms of rehabilitation of the defect and improvement of mastication, deglutition, speech, and esthetics.
제3차 대한간학회 춘계학술대회 초록집 : 포스터연제 토의 ; 만성 간질환에 있어서 비침습적 검사들의 상관관계와 임상적 의의
손주현 ( Son Ju Hyeon ),전용철 ( Jeon Yong Cheol ),윤병철 ( Yun Byeong Cheol ),최호순 ( Choe Ho Sun ),기춘석 ( Gi Chun Seog ),박경남 ( Park Gyeong Nam ),이민호 ( Lee Min Ho ),이재원 ( Lee Jae Won ),박근태 ( Park Geun Tae ),정진웅 대한간학회 1997 Clinical and Molecular Hepatology(대한간학회지) Vol.3 No.2(S)
이산화탄소에서 메탄올로의 전환을 위한 CuO-ZnO/TCP 촉매의 최적 반응조건에 관한 연구
안원주 ( Won-ju Ahn ),이환규 ( Hwan-gyu Lee ),이영호 ( Young-ho Lee ),손석환 ( Seok-hwan Son ),정운조 ( Woon-jo Jeong ),정민철 ( Min-chul Chung ),박권필 ( Kwon-pil Park ),안호근 ( Ho-geun Ahn ) 한국환경기술학회 2014 한국환경기술학회지 Vol.15 No.3
이산화탄소의 온실효과는 세계에서 가장 심각한 문제점 중의 하나로 인식되어 왔다. 메탄올은 화학산업에서 기초적인 중간생성물 중의 하나이며, 또한 연료 첨가제 및 청정 연소 연료로서 사용되어지고 있다. 본 연구에서는 메탄올로의 이산화탄소의 전환 방법을 촉매 화학적 방법을 이용하여 조사하였다. 티타늄 칩 플레이트는 촉매 지지체로서 사용되었다. CuO-ZnO/TCP 촉매는 Wash-coat방법을 이용하여 제조하였다. 제조된 촉매는 SEM과 XRD을 이용하여 담지상태를 조사하였다. 메탄올로의 이산화탄소 수소화에 대한 촉매의 활성은 다양한 반응온도, 반응압력 및 담지 횟수 등을 바꿔가며 유통식 반응기를 이용하여 조사하였다. SEM 이미지와 XRD 패턴으로부터 CuO와 ZnO촉매성분은 Titanium Chip Plate의 표면에 균일하게 잘 담지되어 있음을 알았다. CuO와 ZnO계 담지촉매를 제조하는데 있어서, 전구체 수용액 25.7%(w/v)를 이용한 3회 담지가 가장 높은 효율을 보였으며, 1회 담지를 위한 전구체의 농도는 51.4%(w/v)가 적절하였다. 이산화탄소의 수소화의 활성은 반응온도 210℃에서 최대이고, 반응온도가 250℃ 이상으로 증가하면 역수성 가스 전환반응으로 전환하기 때문에 감소하는 경향을 보였다. 메탄올의 선택도는 반응압력 20atm에서 가장 높게 나타내었으며, 20atm 이상에서는 감소하는 경향을 나타내었다. 결과적으로, 210℃의 CuO-ZnO/TCP 촉매상에서 이산화탄소의 전화율은 27.2%이었고, 최대수율은 13.8%임을 알았다. The greenhouse effect of carbon dioxide has been recognized as one of the most serious problems in the world. Methyl alcohol is one of the basic intermediates in the chemical industry and is also being used as a fuel additive and as a clean burning fuel. In this study, conversion of carbon dioxide to methyl alcohol was investigated using catalytic chemical methods. Titanium chip plate(TCP) was used as a catalyst support. Titanium chip plate(TCP) supported CuO-ZnO catalysts were prepared by wash-coat method. The prepared catalysts were characterized by using SEM and XRD. The catalytic activity for carbon dioxide hydrogenation to methyl alcohol was investigated using a flow-type reactor under various reaction temperatures, reaction pressures and the numbers of impregnation. SEM images and XRD patterns showed that CuO and ZnO was uniformly supported on TCP. In the preparation of CuO-ZnO/TCP catalyst, the appropriate number of impregnation by the precursor solution of 25.7%(w/v) and 51.4%(w/v) concentration were 3 times and a time, respectively. The optimum reaction temperature in the hydrogenation was 210℃, and the selectivity to methyl alcohol was maximum at reaction pressure of 20atm. As the results. CO<sub>2</sub> conversion and yield to methyl alcohol were 27.2% and 13.8% over CuO-ZnO/TCP catalyst at 210℃.
Phodococcus sp. EL-GT 와 Sludge 를 이용한 호기성 생물막 반응기의 특성 및 페놀 처리
박근태,원성내,조순자,손홍주,이건,이상준 한국환경과학회 2002 한국환경과학회지 Vol.11 No.6
The research was performed to compare to the biofilm characteristics and phenol removal efficiency in RBCs(Rotating Biological Contactor) using Rhodococcus sp. EL-GT(single population) and activated sludge(mixed population) as inoculum. Both reactors showed similar tendency on variations of dry weight, thickness and dry density of biofilm. However, the growth of biofilm thickness in 3 and 4 stage of single population reactor has sustained longer than that of the mixed population reactor. Unlike the mixed population reactor, the dry density of biofilm in the single population reactor had a difference between 1, 2 stage and 3, 4 stage. The single population reactor was stably operated without the decrease of phenol removal efficiency in the range of pH 6∼9 and 15mM phenol was completely degraded in these pH ranges. But in case of the mixed population reactor, the phenol degradability was dramatically decreased at over 5mM phenol concentration because of the overgrowth and detachment of its biofilm.