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( Sooin Seo ),( Kwang-woong Lee ),( Seung Cheol Oh ),( Min Young Park ),( Sohee Kim ),( Nam-joon Yi ),( Kyung-suk Suh ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1
Purpose: Hepatitis B immunoglobulin (HBIG) is used long time for prevention of hepatitis B virus (HBV) recurrence after liver transplantation. The HBIG is thought to bind and neutralize with virions or particles which have hepatitis B virus surface antigen (HBsAg) in serum. But according to more recent studies, investigated in vitro HBsAg specific immunoglobulin G (IgG) is internalized in hepatocytes. And HBIG can clearance to HBV with endocytosis in Fc receptors for IgG (FcRn) expression cell lines. The aim of this study was to investigate further mechanism of intracellular action of intravenouse human hepatitis B virus immunoglobulin (I.V. Hepabig) and Hepabig-gene in sense of more specific interaction with HBsAg. With respect to its mechanism of action, Hepabig or Hepabig-gene can effectively promote passive immunization for individuals exposed to the HBV by binding to HBsAg and reducing rate of replication. Methods: The cell lines used in this study were: Huh7, HepG2, HepG2.2.15 (HBV-positive, HBsAg-positive) and PLC/PRF/5 (HBsAg positive). Human primary hepatocytes were isolated from resected partial liver. A variety of cell lines and isolated hepatocytes were exposed to 1) I.V.Hepabig, 2) recombinant Hepabig-gene and 3) Fab portion of Hepabig-gene for 1 hour. Confocal fluorescence microscopy was used to localize HBsAg specific IgG. Western blot analysis for the level of endogenous LC3 and HBsAg proteins was performed to identify autophage. Results: HBsAg was colocalized with I.V. Hepabig, Hepabig-gene or Fab type in the cytoplasm as a punctate pattern of immunofluorescence in HBsAg expression cell lines (HepG2.2.15 and PLC/PRF/5). I.V. Hepabig also localized in cytoplasm with HBsAg in isolated primary hepatocyte from HBsAg positive human liver tissue. Western blot analysis proved that I.V. Hepabig and Hepabig-gene treated hepatocytes accumulated more intracellular HBsAg than control, but not in Fab type of Hepabig-gene treated hepatocytes. Especially, LC3-II which is lipidation of LC3-I form was detected with just Hepabig-gene treatment samples. Conclusion: These results suggest that I.V. Hepabig and Hepabig-gene are present in FcRn expression hepatoma cell lines and primary hepatocytes by endocytosis and colocalized with HBsAg in the cytoplasm. Furthermore, the immunoglobulin-sAg complex induced autophgosom in the cytoplasm.
Choi Seung Woo,Seo Sooin,Hong Hye Kyoung,Yoon So Jung,Kim Minah,Moon Sunghyun,Lee Joo Yong,Lim Jaeseung,Lee Jong Bum,Woo Se Joon 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.6
BACKGROUND: Retinal degenerative disease (RDD), one of the most common causes of blindness, is predominantly caused by the gradual death of retinal pigment epithelial cells (RPEs) and photoreceptors due to various causes. Cell-based therapies, such as stem cell implantation, have been developed for the treatment of RDD, but potential risks, including teratogenicity and immune reactions, have hampered their clinical application. Stem cell-derived extracellular vesicles (EVs) have recently emerged as a cell-free alternative therapeutic strategy; however, additional invasiveness and low yield of the stem cell extraction process is problematic. METHODS: To overcome these limitations, we developed therapeutic EVs for the treatment of RDD which were extracted from tonsil-derived mesenchymal stem cells obtained from human tonsil tissue discarded as medical waste following tonsillectomy (T-MSC EVs). To verify the biocompatibility and cytoprotective effect of T-MSC EVs, we measured cell viability by co-culture with human RPE without or with toxic all-trans-retinal. To elucidate the cytoprotective mechanism of T-MSC EVs, we performed transcriptome sequencing using RNA extracted from RPEs. The in vivo protective effect of T-MSC EVs was evaluated using Pde6b gene knockout rats as an animal model of retinitis pigmentosa. RESULTS: T-MSC EVs showed high biocompatibility and the human pigment epithelial cells were significantly protected in the presence of T-MSC EVs from the toxic effect of all-trans-retinal. In addition, T-MSC EVs showed a dosedependent cell death-delaying effect in real-time quantification of cell death. Transcriptome sequencing analysis revealed that the efficient ability of T-MSC EVs to regulate intracellular oxidative stress may be one of the reasons explaining their excellent cytoprotective effect. Additionally, intravitreally injected T-MSC EVs had an inhibitory effect on the destruction of the outer nuclear layer in the Pde6b gene knockout rat. CONCLUSIONS: Together, the results of this study indicate the preventive and therapeutic effects of T-MSC EVs during the initiation and development of retinal degeneration, which may be a beneficial alternative for the treatment of RDD.
Kim, Sung Han,Seo, Ho Kyung,Shin, Hee Chul,Chang, Sung Ja,Yun, Sooin,Joo, Jungnam,Ku, Ja Hyeon,Kim, Hyung Suk,Jeon, Hwang Gyun,Jeong, Byong Chang,Jeong, In Gab,Kang, Seok Ho,Hong, Bumsik The Korean Academy of Medical Sciences 2015 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.30 No.8
<P>We investigated trends in perioperative chemotherapy use, and determined factors associated with neoadjuvant chemotherapy (NAC) and adjuvant chemotherapy (AC) use in Korean patients with muscle-invasive bladder cancer (MIBC). We recruited 1,324 patients who had MIBC without nodal invasion or metastases and had undergone radical cystectomies (RC) between 2003 and 2013. The study's cut-off time for AC was three months after surgery, and the study's timespan was divided into three periods based on NAC use, namely, 2003-2005, 2006-2009, and 2010-2013. Complete remission was defined as histologically confirmed T0N0M0 after RC. NAC and AC were administered to 7.3% and 18.1% of the patients, respectively. The median time interval between completing NAC and undergoing RC was 32 days and the mean number of cycles was 3.2. The median time interval between RC and AC was 43 days and the mean number of cycles was 4.1. Gemcitabine and cisplatin were most frequently used in combination for NAC (49.0%) and AC (74.9%). NAC use increased significantly from 4.6% between 2003 and 2005 to 8.4% between 2010 and 2013 (<I>P</I> < 0.05), but AC use did not increase. Only 1.9% of patients received NAC and AC. Complete remission after NAC was achieved in 12 patients (12.5%). Multivariable modeling revealed that an advanced age, the earliest time period analyzed, and clinical tumor stage ≤ cT2 bladder cancer were negatively associated with NAC use (<I>P</I> < 0.05). While NAC use has slowly increased over time, it remains an underutilized therapeutic approach in Korean clinical practice.</P>
직독식 기기를 이용한 양돈작업자의 신체부위별 PM<sub>10</sub> 노출 특성 비교 연구
신소정,김효철,김경란,서민태,박수인,김경민,김경수,Sin, Sojung,Kim, Hyocher,Kim, Kyung-ran,Seo, Mintae,Park, Sooin,Kim, Kyungmin,Kim, Kyungsu 한국산업보건학회 2019 한국산업보건학회지 Vol.29 No.2
Objectives: The purpose of this study was to evaluate the personal exposure to $PM_{10}$ by body parts for the development of dust monitoring wearable device for swine farmers. Methods: Tasks were classified by using motion pictures taken by action cameras attached to swine farmers. Concentrations of $PM_{10}$ were measured by attaching direct-reading instruments at the head, neck and waist of worker. Differences of $PM_{10}$ exposure between body parts were analyzed with linear regression. Results: We identified three tasks(vaccination, moving pigs, and manure treatment). $PM_{10}$ concentration during vaccination was the highest among the tasks, and the body part showing the highest concentration of $PM_{10}$ was the waist regardless of task. In all tasks, the closer distance between the body parts, the higher were the R-squared values(vaccination 0.4221, moving pigs 0.6990, and manure treatment 0.2164). Conclusions: We presumed that $PM_{10}$ concentrations were affected by the parts of the body in which they were measured. In order to develop swine farmer's wearable device for monitoring dust concentration in air, the determination of the positions of monitoring sensor to ensure accurate measurement is essential. Considering the results of this study, wearable sensor should be positioned at the waist.
( Tae Yoo ),( Kwang-woong Lee ),( Nam-joon Yi ),( Suk Kyun Hong ),( Jeong-moo Lee ),( Jieun Lim ),( Sooin Seo ),( Kyung-suk Suh ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1
Aims: We aimed to evaluate the association between PNPLA3 polymorphism and post-liver transplantation (LT) outcomes related to alcohol relapse (AR). Methods: We retrospectively analyzed data from patients receiving LT for alcoholic liver disease (ALD) from 04/2014 to 12/2017. Liver-related clinical outcomes were assessed by the gamma-glutamyltransferase (GGT) level and alcohol-related liver failure (ARLF). Genotyping was performed using prospectively collected DNA samples in both donors and recipients. Results: A total of 83 recipients were enrolled. Post-LT AR occurred in 31 patients (37.3%). Thirty-one patients (14 AR, 9 abstainers) showed elevated GGT levels, and 3 AR patients experienced ARLF. In the multivariate analysis, rs738409 G allele carrier and heavy drinking (HRAR score≥4) were independent risk factors for elevated GGT levels (odds ratio [OR]=8.69, P<0.01; OR=13.07, P=0.01) and ARLF (OR=4.52, P=0.04; OR=19.62, P=0.03). Among 15 heavy AR patients, being an rs738409 G allele carrier was related to GGT elevation (P=0.03) and ARLF (P=0.04), but it was not to GGT elevation in mild drinkers (n=16) or abstainers (n=52). Conclusions: PNPLA3 polymorphism of the recipient genotype can independently affect the post-LT prognosis of LT patients for ALD, especially in heavy AR patients. Therefore, strong abstinence education is recommended in patients with this single nucleotide polymorphism.