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Isolation and Characterization of Salt Tolerant Mutations in Budding Yeast Saccharomyces cerevisiae
Seo, Soo Boon,Park, Shi-Young,Kim, Yung-Jin 부산대학교 유전공학연구소 1999 분자생물학 연구보 Vol.15 No.-
In order to study the mechanism for the adaptation to salt stress, we mutagenized budding yeast Saccharomyces cerevisiae with Ethylmethane sulfonate, and isolated salt-tolerant mutants. Among the salt-tolerant mutants, two strains exhibit additional temperature sensitive phenotype. Here, we report that these two salt-tolerant mutants are specific to report that these two salt-tolerant mutants are specific to Na^+ rather than general osmotic stress. These mutant strains may contain mutations in the genes involved in Na^+ homeostasis.
Park, Shi-Young,Seo, Soo Boon,Lee, Soo-Jung,Na, Jong-Gil,Kim, Yung-Jin 부산대학교 유전공학연구소 2001 분자생물학 연구보 Vol.17 No.-
Sodium tolerance in yeast is enhanced by continuous activation of calcineurin, a Ca^2+/calmodulin-dependent protein phosphatase that is required for modulation of the Na^+ efflux mechanism. We isolated several salt-tolerant mutations with the treatment of ethylmethane sulfonate under high salt stress. One of the mutations was mapped in the PMR1 gene. Pmr1p, the P-type Ca^2+ -ATPase in the Colgi apparatus, regulates a cytosolic Ca^2+ level in various responses. Cytosolic Ca^2+ concentration in the pmr1 mutant is highly maintained, and thus calcineurin is activated continuously. The treatment of FK506, a specific inhibitor of calcineurin, abolishes the salt-tolerant phenotype of the pmr1 mutant. Activates caicineurin induces the expression of PMR2, encoding the P-type Na^+-ATPase, through the specific transcription factor, Tcn1p/Crzlp. Also, experssion of the PMR2::lacZ reporter gene in the pmr1 higher than that in wild type. We propose that the pmr1 mutation confers salt tolerance through continuous activation of calcineurin and that Pmr1p might act as a major Ca^2+-ATPase under high salt stress.
Analysis of Expressed Sequence Tags of Porphyra yezoensis
Lee, Eun Kyoung,Seo, Soo Boon,Kim, Tae Hoon,Sung, Soon-Kee,An, Gynheung,Lee, Choon-Hwan,Kim, Yung-Jin 부산대학교 유전공학연구소 2000 분자생물학 연구보 Vol.16 No.-
Single direct partial sequencing of anonymous cDNA clones was performed to obtain information on red algae Porphyra yezoensis of which genetic information is not available. This expressed sequence tags (EST) analysis revealed 81 clones (42%) had significant homologies to known algal genes, whereas above 90% of the EST clones were newly identified in algae. Putative functional categories of these clones showed that the most abundant genes were involved in stress and defense mechanisms and that the next abundant genes were associate with protein synthetic pathways.
Isolation and Characterization of Salt Tolerant Mutations in Budding Yeast Saccharomyces cerevisiae
Kim, Yung-Jin,Seo, Soo-Boon,Park, Shi-Young Korean Society of Life Science 1999 Journal of Life Science Vol.9 No.1
In order to study the mechanism for the adaptation to salt stress, we mutagenized budding yeast Saccharomyces cerevisiae with Ethylmethane sulfonate, and isolated salt-tolerant mutants. Among the salt-tolerant mutants, two strains exhibit additional temperature sensitive phenotype. Here, we report that these two salt-tolerant mutants are specific to {TEX}$Na^{+}${/TEX} rather than general osmotic stress. These mutant strains may contain mutations in the genes involved in {TEX}$Na^{+}${/TEX} home-ostasis.
김지영(Ji Young Kim),서수분(Soo Boon Seo),박찬원(Chan Won Park),이동현(Dong Hyun Lee),주형준(Hyung Joon Ju),강대환(Dae Hwan Kang),조몽(Mong Cho),양웅석(Ung Suk Yang) 대한내과학회 2002 대한내과학회지 Vol.62 No.2
N/A Background: Tissue factor (TF) is a main physiological initiator of blood coagulation and may be important in the biology of a variety of solid malignancies, particularly where angiogenesis is a critical factor. In liver cirrhosis, hyperfibrinolysis is frequently observed in patients with the decompensated state and which may be secondary to the increased production of thrombin. Many studies have shown that the expression of TF in tumors contributes not only to tumor-derived procoagulant activity but also to hematogenous metastasis and tumor angiogenesis. The aim of this study was to evaluate how plasma TF concentration was correlated with the severity of liver cirrhosis and the progression of hepatocellular carcinoma. Methods: This study consisted of 23 patients with liver cirrhosis (LC) and 37 patients with hepatocellular carcinoma (HCC). HCC patients was graded by TNM staging system of The Union Internationale Contre le Cancer (UICC). Plasma TF concentration was measured by the enzyme linked immunosorbent assay (ELISA). The severity of the liver disease was estimated by Child-Pugh classification. The correlation of TF concentration in patients with LC and HCC in diffenrent classifications was evaluated. Results: There was no difference between LC and HCC in the plasma TF concentration (p=0.236). The TF concentration was different according to Child-Pugh score (p=0.024) and proportion to Child-Pugh score in class B and C (p<0.05). The TF concentration of HCC patients was not different between the classes of severity of underlying LC and the TF concentration of stage IVA was higher than that of stage I, II, III in HCC patients (p=0.039). Conclusion: The plasma concentration of TF was correlated with the degree of severity of liver disease in cirrhotic patients and increased in the advanced stages of HCC.(Korean J Med 62:153-158, 2002)
김광하 ( Gwang Ha Kim ),( Dong Hyun Lee ),( Hyung Wook Kim ),( Jong Yun Cheong ),( Soo Boon Seo ),( Jeong Heo ),( Dae Hwan Kang ),( Geun Am Song ),( Mong Cho ),( Ung Suk Yang ),( Do Youn Park ),( Mi Ae Y 대한내과학회 2005 The Korean Journal of Internal Medicine Vol.20 No.4
Background : CDX1 and CDX2 are members of the caudal-type homeobox gene family and control the proliferation and differentiation of intestinal mucosal cells. Their expressions are commonly reduced in colorectal cancer, but reports about the relationships between their expressions and clinicopathologic features are rare. The aim of this study was to examine the expressions of CDX1 and CDX2 mRNAs in colorectal cancers and to assess the relationships between their expressions and clinicopathologic features. Methods : CDX1 and CDX2 mRNA expressions were analyzed by real-time polymerase chain reaction in 48 colorectal cancers and in adjacent non-tumorous normal mucosal tissue. Results : CDX1 and CDX2 mRNA expressions were significantly reduced in colorectal cancer tissues versus normal mucosal tissues (p=0.001, p=0.042, respectively). As compared with paired normal mucosal tissues, colorectal tissues showed reduced CDX1 mRNA expression in 64.6% (31/48) and reduced CDX2 mRNA expression in 66.7% (32/48) of cases. A statistically significant positive correlation was found between the expressions of CDX1 mRNA and CDX2 mRNA in colorectal cancer (r=0.543, p<0.001). However, the expressions of CDX1 and CDX2 mRNAs were not related to age, sex, cancer location, differentiation, lymphatic or vascular invasion, lymph node metastasis, stage or serum carcinoembryonic antigen level. Conclusions : CDX1 and CDX2 mRNA expressions were found to be significantly reduced in colorectal cancers, but these expressional changes were not found to be related to clinicopathologic features.