Acute mitral valve dysfunction and cardiogenic shock due to escape of prosthetic mechanical leaflet

( Mirae Lee ),( Su A Jo ),( Ik Hyun Park ) 대한내과학회 2020 The Korean Journal of Internal Medicine Vol.35 No.1

MicroRNA super-resolution imaging in blood for Alzheimer’s disease

Mirae Lee,Jiwon Woo,Sang Tae Kim,Minho Moon,Sang Yun Kim,Hanna Cho,Sujin Kim,Han-Kyeol Kim,박정윤 생화학분자생물학회 2023 BMB Reports Vol.56 No.3

We propose a novel blood biomarker detection method thatuses miRNA super-resolution imaging to enable the early diagnosisof Alzheimer’s disease (AD). Here, we report a singlemoleculedetection method for visualizing disease-specificmiRNA in tissue from an AD mice model, and peripheral bloodmononuclear cells (PBMCs) from AD patients. Using optimizedMagnified Analysis of Proteome (MAPs), we confirmed that fivemiRNAs contribute to neurodegenerative disease in the brainhippocampi of 5XFAD and wild-type mice. We also assessedPBMCs isolated from the whole blood of AD patients and ahealthy control group, and subsequently analyzed those samplesusing miRNA super-resolution imaging. We detected moremiR-200a-3p expression in the cornu ammonis 1 and dentategyrus regions of 3 month-old 5XFAD mice than in wild-typemice. Additionally, miRNA super-resolution imaging of bloodprovides AD diagnosis platform for studying miRNA regulationinside cells at the single molecule level. Our results present apotential liquid biopsy method that could improve the diagnosisof early stage AD and other diseases.

Enhancement of Photocurrent Generation by C60-encapsulated Single-walled Carbon Nanotubes in Ru-sensitized Photoelectrochemical Cell

Jungwoo Lee,Taehee Park,Jongtaek Lee,Mira Jang,Seungjin Lee,Heesu Kim,한성환,이휘건 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.8

Single-walled carbon nanotubes (SWNTs) and C60-encapsulated SWNTs (C60@SWNTs) are introduced to Rusensitized photoelectrochemical cells (PECs), and photocurrents are compared between two cells, i.e., an RuL2(NCS)2/DAPV/SWNTs/ITO cell and an RuL2(NCS)2/DAPV/C60@SWNTs/ITO cell. [L = 2,2'- bipyridine-4,4'-dicarboxylic acid, DAPV = di-(3-aminopropyl)-viologen, and ITO = indium-tin oxide] The photocurrents are increased by 70.6% in the presence of C60@SWNTs. To explain the photocurrent increase, the reverse-field emission method is used, i.e., RuL2(NCS)2/DAPV/SWNTs/ITO cell (or RuL2(NCS)2/DAPV/ C60@SWNTs/ITO cell) as an anode and a counter electrode Pt as a cathode in the external electric field. The improved field emission properties, i.e., β (field enhancement factor) and emission currents in the reverse-field emission with C60@SWNTs indicate the enhancement of the PEC electric field, which implies the improvement of the electron transfer rate along with the reduced charge recombination in the cell.

일반 검색광고와 쇼핑 검색광고 효과에 영향을 미치는 소비자 특성과 지각된 검색광고 특성: 미국 소비자를 대상으로

이미라(Mira Lee),이현경(Hyun-Kyung Lee) 한국OOH광고학회 2020 OOH광고학연구 Vol.17 No.3

검색광고는 검색엔진에 소비자가 입력한 검색어에 대응하여 검색결과 페이지에 노출되는 광고이다. 본 연구는 미국 소비자를 대상으로 설문조사를 통하여 구글 검색 사이트에서 제공하는 일반 검색광고와 쇼핑 검색광고의 태도, 클릭빈도 및 구매빈도에 영향을 미치는 소비자 특성과 지각된 검색광고 특성에 대해 살펴보았다. 소비자 특성에 관한 연구결과, 브랜드의식은 쇼핑 검색광고 효과에 정의 영향을 미치는 것으로 나타났다. 충동구매성향은 검색광고 클릭빈도와 구매빈도에 정의 영향을 미쳤다. 마지막으로, 광고회의가 높을수록 검색광고 태도가 더 부정적이었으며 클릭빈도와 구매빈도가 더 낮았다. 지각된 검색광고 특성에 관한 연구결과, 지각된 광고의 정보성은 검색광고 태도에 정의 영향을 미쳤지만, 지각된 침입성은부의 영향을 미치는 것으로 나타났다. 마지막으로 지각된 검색광고 과부하는 검색광고 클릭빈도와 구매빈도에 부의 영향을 미쳤다. Search advertising is displayed on a search result page in response to a consumerʼs search query. Using a survey method, this study examined the factors influencing the effectiveness of typical search advertising and shopping search advertising. Specifically, this study examined the influences of consumer characteristics and perceived search advertising characteristics on American consumersʼ attitude toward search advertising, click frequency, and purchase frequency. Regarding the consumer characteristics, this study demonstrates that brand consciousness influences attitude toward shopping search advertising, click frequency, and purchase frequency positively. Impulse buying tendency has positive effects on both click frequency and purchase frequency. Moreover, the more consumers are skeptical about advertising in general, the more negatively they respond to search advertising. For the perceived search advertising characteristics, this study shows that perceived informativeness influences attitude toward search advertising positively. On the other hand, perceived intrusiveness has a negative influence on search advertising attitude. Finally, search advertisingʼs perceived overload influences both click frequency and purchase frequency negatively.

Purification of multi-serotype of AAV with modified PCL system

Heehyung LEE,Slgirim LEE,Seung-Hyun KIM,Mira CHO,Yoojin KIM,Seokmin OH,Seungju YOO,Nara LEE,Ginah HAN,Jae-Hyung JANG 한국생물공학회 2018 한국생물공학회 학술대회 Vol.2018 No.4

The Complete Plastome Sequence of an Antarctic Bryophyte <i>Sanionia uncinata</i> (Hedw.) Loeske

Park, Mira,Park, Hyun,Lee, Hyoungseok,Lee, Byeong-ha,Lee, Jungeun MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.3

<P>Organellar genomes of bryophytes are poorly represented with chloroplast genomes of only four mosses, four liverworts and two hornworts having been sequenced and annotated. Moreover, while Antarctic vegetation is dominated by the bryophytes, there are few reports on the plastid genomes for the Antarctic bryophytes. <I>Sanionia uncinata</I> (Hedw.) Loeske is one of the most dominant moss species in the maritime Antarctic. It has been researched as an important marker for ecological studies and as an extremophile plant for studies on stress tolerance. Here, we report the complete plastome sequence of <I>S</I>. <I>uncinata</I>, which can be exploited in comparative studies to identify the lineage-specific divergence across different species. The complete plastome of <I>S</I>. <I>uncinata</I> is 124,374 bp in length with a typical quadripartite structure of 114 unique genes including 82 unique protein-coding genes, 37 tRNA genes and four rRNA genes. However, two genes encoding the <I>α</I> subunit of RNA polymerase (<I>rpoA</I>) and encoding the cytochrome b<SUB>6/f</SUB> complex subunit VIII (<I>petN</I>) were absent. We could identify nuclear genes homologous to those genes, which suggests that <I>rpoA</I> and <I>petN</I> might have been relocated from the chloroplast genome to the nuclear genome.</P>

Isolation and Characterization of Listeria phages for Control of Growth of Listeria monocytogenes in Milk

Sunhee Lee,Min Gon Kim,Hee Soo Lee,Sunhak Heo,Mirae Kwon,Geun- Bae Kim 한국축산식품학회 2017 한국축산식품학회지 Vol.37 No.2

In this study, two Listeria bacteriophages, LMP1 and LMP7, were isolated from chicken feces as a means of biocontrol of L. monocytogenes. Both bacteriophages had a lytic effect on L. monocytogenes ATCC 7644, 15313, 19114, and 19115. Phages LMP1 and LMP7 were able to inhibit the growth of L. monocytogenes ATCC 7644 and 19114 in tryptic soy broth at 10℃ and 30℃. Nevertheless, LMP1 was more effective than LMP7 at inhibiting L. monocytogenes ATCC 19114. On the contrary, LMP7 was more effective than LMP1 at inhibiting L. monocytogenes ATCC 7644. The morphology of LMP1 and LMP7 resembled that of members of the Siphoviridae family. The growth of L. monocytogenes ATCC 7644 was inhibited by both LMP1 and LMP7 in milk; however, the growth of L. monocytogenes ATCC 19114 was only inhibited by LMP1 at 30℃. The lytic activity of bacteriophages was also evaluated at 4℃ in milk in order to investigate the potential use of these phages in refrigerated products. In conclusion, these two bacteriophages exhibit different host specificities and characteristics, suggesting that they can be used as a component of a phage cocktail to control L. monocytogenes in the food industry.

Importance-Performance Analysis와 Borich 요구도 분석 방법을 활용한 임상간호실무 교육 요구도: 일개 상급종합병원 일반병동 간호사 대상

이미라(Lee, Mira),김지영(Kim, Jiyoung),김보연(Kim, Boyeon),박유연(Park, Yooyun),한지유(Han, Jiyoo),이승희(Lee, Seunghee),이현주(Lee, Hyunju) 한국간호교육학회 2023 한국간호교육학회지 Vol.29 No.2

Purpose: This study’s aim was to confirm the importance and performance of the clinical practice of nurses working in the general wards of a tertiary hospital and to analyze their educational needs. Methods: The study employed a descriptive research design, and a self-reported questionnaire was developed and used for nurses in a tertiary hospital. Data were collected from July 22 to July 29, 2022, analyzed by an independent t-test, paired t-test, and one-way ANOVA for the importance and performance of each clinical nursing practice according to general characteristics, and then a post hoc verification was performed by Scheffé’s test. An Importance-Performance Analysis and Borich needs assessment model were used to analyze clinical nursing education needs. Results: Clinical nursing practice performance showed a significant difference according to length of total clinical career, time working in current department, and preceptor experience. According to the results of the Importance-Performance Analysis, neurological evaluation, and nursing intervention, artificial respirator and high-flow oxygen inhalation nursing, chemotherapy, emergency nursing, and cardiopulmonary resuscitation were identified as belonging to the “concentrate here” quadrant. All these items ranked in the top 10 in the Borich needs assessment model. Conclusion: Based on these results, the current education system should be reviewed, and short and long term education strategies based on educational needs should be established to strengthen the competence of nurses.

3D 프린터를 활용한 수직축 헬리컬형 풍력 발전기의 설계 및 제작

김미래(Mirae Kim),이현아(Hyeon A Lee),이연주(Yeon Ju Lee),허효원(Hyo Won Heo),김경천(Kyung Chun Kim) 대한기계학회 2015 대한기계학회 춘추학술대회 Vol.2015 No.11

Design and fabrication of wind turbine have been investigated. As a component of wind turbine, the vertical type wind turbine with helical blades was designed. CFD analysis has performed with the 3D blade model. Small size blades were manufactured using a 3D printer. The performance of the assembled wind turbine was evaluated by the CFD analysis. It is confirmed that the rated power is well agreed with the design parameters. The concept of wind turbine can be applied as a high performance wind power generation system especially in urban environment considering landscape with the limited area.

간호대학생의 임상실습 중 폭력경험과 임상학습환경이 직업정체성에 미치는 영향

이미라(Lee, Mira),박희옥(Park, Hee Ok),이인숙(Lee, Insook) 한국간호교육학회 2021 한국간호교육학회지 Vol.27 No.3

Purpose: The purpose of this study is to determine the influence of the learning environment on nursing students’ clinical practice education and the violence experienced during clinical practice on vocational identity. Methods: The design of the study was a descriptive survey, and data were collected from November 15 to November 27, 2019. The data of the study were obtained from 515 nursing students attending three universities using self-administered questionnaires. Data were analyzed using the SPSS 25.0 program. Results: For the experience of violence, verbal violence (98.3%) was the type most commonly experienced, and patients (97.7%) were the most frequent perpetrators. The clinical learning environment was perceived differently according to gender, personality, interpersonal relationship, satisfaction with nursing, clinical practice satisfaction, violence prevention education, the need for violence prevention education, sexual violence experiences, and violent perpetrators. The most influential factor on vocational identity was satisfaction with the nursing major (β=0.24, p<.001), followed by extroverted personality (β=0.18, p<.001), clinical learning environment (β=0.15, p=.001), satisfaction with clinical practice (β=0.15, p=.002), and the experience of violence by patients (β=-0.10, p=.016), which together explained 24.1% of the variance in the model. Conclusion: It is necessary to make efforts to ensure that students do not experience violence during clinical practice, to maintain a close cooperative relationship between university and clinical institutions to improve the learning environment for clinical practice, and to make the clinical field an educational learning environment.