랜덤화 블록 계획법에서 위치를 이용한 비모수 검정법

심수진,김동재,Sim, Sujin,Kim, Dongjae 한국데이터정보과학회 2013 한국데이터정보과학회지 Vol.24 No.6

반복이 있는 랜덤화 블록 계획법에서 우산형 대립가설을 검정하기 위한 비모수 방법에는 Kim과 Kim (1992)이 제안한 방법이 있다. 본 논문에서는 Orban과 Wolfe (1982)가 제안한 위치와 Kim (1999)이 제안한 대조군과 처리군의 방법을 확장하여 랜덤화 블록 계획법에서 우산형 대립가설에서의 검정법을 제안하였다. 또한 여러 분포에 대한 모의실험 통하여 기존의 방법과의 검정력을 비교하였다. Kim and Kim (1992) proposed typical nonparametric method for umbrella alternative in randomized block design with replications. In this paper, We consider a test procedure for umbrella alternatives in a randomized block design using extension of the two sample placement tests described in Orban and Wolfe (1982) and treatment tests described in Kim (1999). We perform a Monte Carlo study to compare the empirical powers of the test statistics for underlying distributions.

한국어교육에서 다중언어능력 및 다중문화능력의 개념과 적용

조수진 ( Cho¸ Sujin ),김태나 ( Kim¸ Taena ),강승혜 ( Kang¸ Seunghae ) 한국언어문화교육학회 2021 언어와 문화 Vol.17 No.3

This study examines the concept of plurilingual and pluricultural competence presented by the CEFR(2018) and proposes application methods in Korean language education. In Korean language education, learners can improve their competence to target language and culture by understanding that learners have various languages and cultural backgrounds and utilizing such resources and experiences. To this end, the understanding and acceptance of culture can be facilitated by establishing a pluricultural repertoire. The target language can be learned by utilizing various language resources through plurilingual understanding of plurilingualism. Furthermore, by building a plurilingual repertoire, it is possible to take advantage of the language resources that learners have to take the initiative in language learning. By applying this concept, a teaching-learning method can be proposed so that learners can utilize each other's language and cultural resources in professor learning, which used only Korean, the target language in Korean education. (Sogang UniversityㆍSogang UniversityㆍYonsei University)

Age-dependent gait abnormalities in mice lacking the <i>Rnf170</i> gene linked to human autosomal-dominant sensory ataxia

Kim, Youngsoo,Kim, Seong Hun,Kim, Kook Hwan,Chae, Sujin,Kim, Chanki,Kim, Jeongjin,Shin, Hee-Sup,Lee, Myung-Shik,Kim, Daesoo IRL Press 2015 Human molecular genetics Vol.24 No.25

<P>Really interesting new gene (RING) finger protein 170 (RNF170) is an E3 ubiquitin ligase known to mediate ubiquitination-dependent degradation of type-I inositol 1,4,5-trisphosphate receptors (ITPR1). It has recently been demonstrated that a point mutation of <I>RNF170</I> gene is linked with autosomal-dominant sensory ataxia (ADSA), which is characterized by an age-dependent increase of walking abnormalities, a rare genetic disorder reported in only two families. Although this mutant allele is known to be dominant, the functional identity thereof has not been clearly established. Here, we generated mice lacking <I>Rnf170</I> (<I>Rnf170<SUP>−/−</SUP></I>) to evaluate the effect of its loss of function <I>in vivo</I>. Remarkably, <I>Rnf170<SUP>−/−</SUP></I> mice began to develop gait abnormalities in old age (12 months) in the form of asynchronous stepping between diagonal limb pairs with a fixed step sequence during locomotion, while age-matched wild-type mice showed stable gait patterns using several step sequence repertoires. As reported in ADSA patients, they also showed a reduced sensitivity for proprioception and thermal nociception. Protein blot analysis revealed that the amount of Itpr1 protein was significantly elevated in the cerebellum and spinal cord but intact in the cerebral cortex in <I>Rnf170<SUP>−/−</SUP></I> mice. These results suggest that the loss of <I>Rnf170</I> gene function mediates ADSA-associated phenotypes and this gives insights on the cure of patients with ADSA and other age-dependent walking abnormalities.</P>

PKR Senses Nuclear and Mitochondrial Signals by Interacting with Endogenous Double-Stranded RNAs

Kim, Yoosik,Park, Joha,Kim, Sujin,Kim, MinA,Kang, Myeong-Gyun,Kwak, Chulhwan,Kang, Minjeong,Kim, Baekgyu,Rhee, Hyun-Woo,Kim, V. Narry Elsevier 2018 Molecular cell Vol.71 No.6

<P><B>Summary</B></P> <P>Protein kinase RNA-activated (PKR) induces immune response by sensing viral double-stranded RNAs (dsRNAs). However, growing evidence suggests that PKR can also be activated by endogenously expressed dsRNAs. Here, we capture these dsRNAs by formaldehyde-mediated crosslinking and immunoprecipitation sequencing and find that various noncoding RNAs interact with PKR. Surprisingly, the majority of the PKR-interacting RNA repertoire is occupied by mitochondrial RNAs (mtRNAs). MtRNAs can form intermolecular dsRNAs owing to bidirectional transcription of the mitochondrial genome and regulate PKR and eIF2α phosphorylation to control cell signaling and translation. Moreover, PKR activation by mtRNAs is counteracted by PKR phosphatases, disruption of which causes apoptosis from PKR overactivation even in uninfected cells. Our work unveils dynamic regulation of PKR even without infection and establishes PKR as a sensor for nuclear and mitochondrial signaling cues in regulating cellular metabolism.</P> <P><B>Highlights</B></P> <P> <UL> <LI> fCLIP-seq reveals PKR-interacting endogenously expressed dsRNAs </LI> <LI> PKR binds to various noncoding RNAs such as retrotransposons and satellite RNAs </LI> <LI> MtRNAs can form intermolecular dsRNAs and strongly interact with PKR </LI> <LI> MtRNAs can regulate PKR phosphorylation and signaling, especially under stress </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>

Development of Eggshell Membrane/Hydroxyapatite-Based Functional Engineering Platforms for Healthcare Applications

( Sujin Kim ),( Sunho Park ),( Daun Kim ),( Yonghyun Gwon ),( Jangho Kim ) 한국농업기계학회 2019 한국농업기계학회 학술발표논문집 Vol.24 No.2

Eggshell membrane (ESM) is an agricultural by-product and bioresource, and it exists between egg white and eggshell. Recently, ESM is being recognized as a new biomaterial in biomedical fields (e.g., therapeutic agents for skin wound healing). ESM has entangled micro-fibrous structure with micro-pores, and it is mainly composed of abundant proteins, such as collagen I, V, X, and osteopontin. Also, the surfaces of ESM have many functional chemical groups including amine, carboxyl, and amide groups. However, the raw ESM has many limitations of the restricted scale-up platforms, low mechanical property and poor bioactivity. To overcome the limitations of the raw ESM, we proposed new ESM-based functional platforms using nanomaterial for the various applications in the fields of healthcare (e.g., bone tissue engineering). Firstly, we fabricated the ESM solution by dissolving the disulfide bond of the raw ESM for the fabrication of ESM-based platforms with various size. Secondly, we used hydroxyapatite powders (HAp) with nanoscale that can be incorporated into the ESM solution to enhance the mechanical and bioactive property of the raw ESM. Finally, we confirmed that the proliferation and differentiation of the osteoblast-like cells were increased on the HAp/ESM composite with appropriate composite conditions compared to those on the ESM solution. The proposed HAp/ESM composite could be used as new biomaterials for healthcare applications including bone tissue engineering.

Associations of Polyp Characteristics in Children and Adolescents Presenting with Less Than Five Colorectal Polyps: A Full Colonoscopy Is Still Required

Kim Ju Young,Kim Yu Bin,Choi Sujin,Lee Yoo Min,Kim Hyun Jin,Kim Soon Chul,Jang Hyo-Jeong,Choi So Yoon,Yi Dae Yong,Lee Yoon,Choi You Jin,Kang Yunkoo,Lee Kyung Jae,Suk Jin Hong,Hwang Jun Hyun,Kwak Sangg 거트앤리버 소화기연관학회협의회 2023 Gut and Liver Vol.17 No.3

Background/Aims: A full colonoscopy is currently required in children and adolescents with colorectal polyps, because of their potential of neoplastic transformation and complications such as intussusception. We aimed to analyze the associations of polyp characteristics in children and adolescents with colorectal polyps. Based on these findings, we also aimed to reevaluate the necessity of conducting a full colonoscopy. Methods: Pediatric patients <18 years of age who had undergone a colonoscopic polypectomy and those with <5 colorectal polyps were included in this multicenter, retrospective study. Baseline clinicodemographics, colonoscopic and histologic findings were investigated. Results: A total of 91 patients were included. Multivariate logistic regression analysis showed that polyp size was the only factor associated with the presence of any polyps located proximal to the splenic flexure (odds ratio [OR], 2.25; 95% confidence interval [CI], 1.28 to 4.28; p=0.007). Furthermore, polyp location proximal to the splenic flexure and sessile morphology were associated with the presence of any adenomatous polyp (OR, 8.51; 95% CI, 1.43 to 68.65; p=0.023; OR, 18.41; 95% CI, 3.45 to 173.81; p=0.002, respectively). Conclusions: In children and adolescents presenting with <5 colorectal polyps, polyp size and the presence of any adenomatous polyp were positively associated with polyp location proximal to the splenic flexure. This finding supports the necessity of a full colonoscopic exam in pediatric patients with colorectal polyps for the detection of polyps before the occurrence of complications such as intussusception or neoplastic transformation.

Mechanobiological Regulation of Cells by Nanotopographically-defined Biomimetic Scaffolds for Animal Tissue Engineering

( Sujin Kim ),( Sunho Park ),( Daun Kim ),( Woochan Kim ),( Dohyeon Lee ),( Sungmin Park ),( Jangho Kim ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

Living cells are exposed to complex and functional microenvironment including soluble macromolecules, biophysical cues, and interactions between cell-cell and cell-extracellular matrix (ECM). This suggests that the design and manipulation of engineered cellular microenvironments is of great important in a wide variety of biological applications such as fundamental biology, therapeutic and diagnostic research, stem cells and regenerative medicine, and developing in vitro disease models. In this work, we described the development of nanoscale engineered biomimetic systems for animal tissue engineering. Specifically, we showed the nanoscale patterned scaffolds inspired by the unique architectures of native animal tissues and ECMs that can provide cells the in vivo-like topographical cell environment, and their applications for better understanding of the function of living animal cells and tissue regeneration without complex surgical treatments.

SARS-CoV-2 mRNA Vaccine Elicits Sustained T Cell Responses Against the Omicron Variant in Adolescents

Sujin Choi,Sang-Hoon Kim,Mi Seon Han,Yoonsun Yoon,Yun-Kyung Kim,Hye-Kyung Cho,Ki Wook Yun,Seung Ha Song,Bin Ahn,Ye Kyung Kim,Sung Hwan Choi,Young June Choe,Heeji Lim,Eun Bee Choi,Kwangwook Kim,Seokhwa 대한면역학회 2023 Immune Network Vol.23 No.4

Vaccination against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been acknowledged as an effective mean of preventing infection and hospitalization. However, the emergence of highly transmissible SARS-CoV-2 variants of concern (VOCs) has led to substantial increase in infections among children and adolescents. Vaccineinduced immunity and longevity have not been well defined in this population. Therefore, we aimed to analyze humoral and cellular immune responses against ancestral and SARSCoV- 2 variants after two shots of the BNT162b2 vaccine in healthy adolescents. Although vaccination induced a robust increase of spike-specific binding Abs and neutralizing Abs against the ancestral and SARS-CoV-2 variants, the neutralizing activity against the Omicron variant was significantly low. On the contrary, vaccine-induced memory CD4+ T cells exhibited substantial responses against both ancestral and Omicron spike proteins. Notably, CD4+ T cell responses against both ancestral and Omicron strains were preserved at 3 months after two shots of the BNT162b2 vaccine without waning. Polyfunctionality of vaccine-induced memory T cells was also preserved in response to Omicron spike protein. The present findings characterize the protective immunity of vaccination for adolescents in the era of continuous emergence of variants/subvariants.

Source Investigation and Control of Ralstonia mannitolilytica Bacteremia in a Neonatal Intensive Care Unit: A Case Report

Kim Dabin,Kim Min Hye,Park Seul Gi,Choi Sujin,Lee Chan Jae,Jung Young Hwa,Choi Chang Won,Shin Myoung-Jin,Song Kyoung-Ho,Kim Eu Suk,Park Jeong Su,Kim Hong Bin,Lee Hyunju 대한소아감염학회 2023 Pediatric Infection and Vaccine Vol.30 No.1

A case of persistent Ralstonia mannitolilytica bacteremia in the neonatal intensive care unit prompted source investigation due to its rarity. After an extensive investigation, a contaminated ultrasonic nebulizer was identified as the source, and the infection was controlled by removing the source. This study emphasizes the importance of further investigations, even in single cases of rare pathogens.

Potential Utility of Fecal Calprotectin in Discriminating Colorectal Polyps From Other Major Etiologies in Children Presenting With Isolated Hematochezia

Kim Yu Bin,Kim Ju Young,Choi Sujin,Kim Hyun Jin,Lee Yoo Min,Lee Yoon,Jang Hyo-Jeong,Lee Eun Hye,Lee Kyung Jae,Kim Soon Chul,Choi So Yoon,Kang Yunkoo,Yi Dae Yong,Choi You Jin,Choe Byung-Ho,Kang Ben 대한의학회 2022 Journal of Korean medical science Vol.37 No.9

Background: Colorectal polyps are the most common cause of isolated hematochezia in children, which requires a colonoscopy for diagnosis. We aimed to investigate the potential utility of fecal calprotectin (FC) in assessing colorectal polyps detected by colonoscopy among children presenting with isolated hematochezia. Methods: Pediatric patients of the age of < 18 years who had undergone both colonoscopy and FC tests for isolated hematochezia from June 2016 to May 2020 were included in the present multicenter, retrospective, cross-sectional study. Comparative analysis was conducted between major causes of isolated hematochezia and FC cut-offs for discriminating colorectal polyps were explored. Results: A total 127 patients were included. Thirty-five patients (27.6%) had colorectal polyps, followed by anal fissure (14.2%), ulcerative colitis (UC; 12.6%), and others. A significant difference in FC levels was observed between patients with colorectal polyps (median, 278.7 mg/kg), anal fissures (median, 42.2 mg/kg), and UC (median, 981 mg/ kg) (P < 0.001). According to receiver operating characteristic curve analysis, among patients diagnosed with colorectal polyp or anal fissure, the most accurate FC cut-off for discriminating colorectal polyps from anal fissures on colonoscopy was 225 mg/kg (sensitivity, 59.4%; specificity, 94.4%; positive predictive value [PPV], 95.0%; negative predictive value [NPV], 56.7%; area under the curve [AUC], 0.8; 95% confidence interval [CI], 0.678–0.923; P < 0.001), while among patients diagnosed with colorectal polyp or UC, the most accurate FC cut-off for discriminating colorectal polyps from UC on colonoscopy was 879 mg/kg (sensitivity, 81.2%; specificity, 56.2%; PPV, 78.8%; NPV, 60.0%; AUC, 0.687; 95% CI, 0.521–0.852; P < 0.001). Conclusion: FC may assist in assessing the cause of lower gastrointestinal tract bleeding in children who present with isolated hematochezia.