The USP21/YY1/SNHG16 axis contributes to tumor proliferation, migration, and invasion of non-small-cell lung cancer

Pei Xu,Haibo Xiao,Qi Yang,Rui Hu,Lianyong Jiang,Rui Bi,Xueyan Jiang,Lei Wang,Ju Mei,Fangbao Ding,Jianbing Huang 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

Deubiquitinases (DUBs) and noncoding RNAs have been the subjects of recent extensive studies regarding their roles in lung cancer, but the mechanisms involved are largely unknown. In our study, we used The Cancer Genome Atlas data set and bioinformatics analyses and identified USP21, a DUB, as a potential contributor to oncogenesis in nonsmall-cell lung cancer (NSCLC). We further demonstrated that USP21 was highly expressed in NSCLCs. We then conducted a series of in vitro and in vivo assays to explore the effect of USP21 on NSCLC progression and the underlying mechanism involved. USP21 promoted NSCLC cell proliferation, migration, and invasion and in vivo tumor growth by stabilizing a well-known oncogene, Yin Yang-1 (YY1), via mediating its deubiquitination. Furthermore, YY1 transcriptionally regulates the expression of SNHG16. Moreover, StarBase bioinformatics analyses predicted that miR4500 targets SNHG16 and USP21. A series of in vitro experiments indicated that SNHG16 increased the expression of USP21 through miR-4500. In summary, the USP21/YY1/SNHG16 axis plays a role in promoting the progression of NSCLC. Therefore, the USP21/YY1/SNHG16/miR-4500 axis may be a potential therapeutic target in NSCLC treatment.

Improved Work Function of Preferentially Oriented Indium Oxide Films Induced by the Plasma Exposure Technique

Lei Yang,Shuai Guo,Qiuling Yang,Yuankun Zhu,Bing Dai,Hailing Yu,Pei Lei,Jiecai Han,Ying Hou,Jiaqi Zhu 대한금속·재료학회 2015 ELECTRONIC MATERIALS LETTERS Vol.11 No.6

The preferentially oriented In2O3 thin films were prepared on glass substrates by conventional magnetron sputtering with Ar+ plasma exposure at room temperature. Based on the x-ray diffraction, x-ray photoelectron spectroscopy, and UV photoelectron spectroscopy results, it was found that the Ar+ plasma exposure not only enhanced the low-temperature crystallization of In2O3 thin films, but also led to a dramatic improvement in the work function. Furthermore, it demonstrated that the shift mechanism of the work function in In2O3 thin films mainly combined with theelimination of oxygen defects and the change of the preferential orientation of In2O3 film surface.

A novel recyclable nano-adsorbent for enhanced oil recovery with efficient removal of Ca2+ and Cr6+ from oilfield wastewater

Lei He,Yong Dai,Zhe Wang,Lutao Yang,Luxia Zhang,Pengpeng Hu,Yutian Lei,Hong Mo,Haomiao Zhu,Jun Zhang 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.8

Oilfield wastewater has a significantly negative influence on the recovery efficiency due to its hypersalinity. Therefore, magnetic graphene oxide (GO) modified with ethylene diamine tetraacetic acid (EDTA) and grafted bypolyethylene glycol (PEG) nano-adsorbent was prepared to remove the Ca2+ and Cr6+ in Jidong Oilfield wastewaterspecifically. It was noteworthy that PEG-g-EDTA/Fe3O4/GO nano-adsorbent had excellent adsorption performance,with removal ratios up to 71.6% for Ca2+ and 42.7% for Cr6+ at 30 min. After the fifth recycling run, the nano-adsorbentstill remained with high removal ratios (69.3% for Ca2+ and 30.5% for Cr6+) and high reuse rates (89.4% for Ca2+and 95.2% for Cr6+), indicating that the nano-adsorbent had outstanding adsorption-desorption efficacy and high reusability. The adsorption test results demonstrated that the total salinity of the treated oilfield wastewater decreased byabout 5,000 ppm, and the displacement efficiency further increased by 12.5% by chemical flooding compared with theuntreated wastewater. Therefore, PEG-g-EDTA/Fe3O4/GO nano-adsorbent is an adsorbent with excellent potentialwhich can effectively remove Ca2+ and Cr6+ in oilfield wastewater to enhance oil recovery (EOR).

A physics-based three dimensional readout model for phase-change probe memory

Lei Wang,C.D. Wright,M.M. Aziz,C.-H. Yang,G.-W. Yang 한국물리학회 2014 Current Applied Physics Vol.14 No.9

A physics-based three dimensional model is developed for the first time to assess the readout performance of phase-change probe memory. The isolated bit responses for reading a crystalline bit with an amorphous background and an amorphous bit with a crystalline background are investigated using this model under a calculated safe readout potential, resulting in a practicable readout current. The readout performances of multiple bit arrays for both cases are also evaluated to establish the influence of noise sources on the readout signal in terms of the inter-track interference and the inter-symbol interference. The results reveal that the configuration having an amorphous bit with a crystalline background exhibits a better anti-interference characteristic than the crystalline counterpart.

KDM3A promotes oral squamous cell carcinoma cell proliferation and invasion via H3K9me2 demethylation-activated DCLK1

Yang Lei,Zhang Qiqiong,Yang Qiuye 한국유전학회 2022 Genes & Genomics Vol.44 No.11

Background: Oral squamous cell carcinoma (OSCC) is a frequently-diagnosed malignancy with high potential for proliferation and invasion. Histone methylation is known as a crucial mechanism that regulates pathological processes in various cancers, including OSCC. Objective: This study sought to delve into the molecular mechanism of lysine demethylase 3 A (KDM3A) in OSCC cell proliferation and invasion. Methods: Expression levels of KDM3A, lysin-9 of di-methylated histone H3 (H3K9me2), and doublecortin-like kinase 1 (DCLK1) in cells were determined by reverse-transcription quantitative polymerase chain reaction or Western blot analysis. Cell proliferation and invasion were evaluated by cell counting kit-8, colony formation, and Transwell assays. The enrichment of KDM3A and H3K9me2 on the DCLK1 promoter was determined by chromatin immunoprecipitation assay. The functional rescue experiment was performed with DCLK1 overexpression vector and si-KDM3A in CAL-27 and SCC-9 cells. Results: KDM3A was elevated in OSCC cells. KDM3A knockdown suppressed OSCC proliferation and invasion, along with increased H3K9me2 level in OSCC cells. KDM3A and H3K9me2 were enriched on the DCLK1 promoter and inhibiting H3K9me2 improved DCLK1 expression levels. DCLK1 overexpression neutralized the inhibition of KDM3A knockdown on OSCC proliferation and invasion. Conclusions: KDM3A facilitated OSCC proliferation and invasion by eliminating H3K9me2 to upregulate DCLK1 expression levels.

Improvement of comprehensive performance of compound green soil in sponge city

Lei Zhang,Zhicheng Li,Tianliang Yang,Ping Yang 대한환경공학회 2021 Environmental Engineering Research Vol.26 No.5

Large-scale constructions of urbanization increase the impervious areas of city, leading to the urban hydrological effects such as urban waterlogging and rainwater runoff pollution. To this end, China proposed to adopt the measure to build sponge cities. However, the existing green soil cannot meet the comprehensive needs of sponge city. In order to quickly evaluate the comprehensive performance of the soil in sponge city, a comprehensive evaluation criterion was designed, which is related to the characteristics of greening soil permeability, porosity, pH, salinity and fertility. Based on the criterion, a new type of composite green soil was obtained with the silt soil: medium sand: sawdust ratio of 72.5%: 20%: 7.5%. Finally, compared with the existing soil, the new green soil not only meets the comprehensive performance requirements of the green soil’s fertility, pH, permeability and other factors, but also has higher permeability and water retention. It was proved that the new green soil has apparent advantages in the control of rainwater.

Simulation Research of Multi-morphological Microstructures Chaining Mechanism of Nano-magnetic Fluid

Lei Yang,Yibiao Chen,Yao Yao,Shuaikang Wei,Yuhui Xie,Decai Li,Jun Yu,Hongming Zhou,Yiping Feng 한국자기학회 2024 Journal of Magnetics Vol.29 No.1

For nano-magnetic fluid(NMF), the magnetic nano-particle(MNP) kinematic behavior in a magnetic field and the chain-like microstructure evolution are important to study the NMF properties at the microscopic level. However, the chain structure formation mechanism and the effects of multiple environmental factors on microstructural morphology remain unclear. In this paper, the interaction of a two-particle system, chain structure formation process, microstructure evolution and response time simulation of NMF is investigated by the discrete element simulation method. The results show that the magnetic dipole and repulsive forces dominate the chain structure formation and evolution under a uniform magnetic field. MNPs assemble into chain-like structures and various complex structures along the magnetic f ield direction. The volume fraction, magnetization intensity and particle size significantly affect the microstructure and the response time. The above study can obtain the specific morphology of the microstructure at different working conditions and broaden the application of NMF in practical engineering.

Optimal Decisions and Coordination Mechanisms for a Fresh Agricultural Product Supply Chain System

Lei Yang,Kebing Chen,Guimei Ma 보안공학연구지원센터 2014 International Journal of u- and e- Service, Scienc Vol.7 No.5

For a one-supplier-one-retailer agricultural product supply chain, quantity and quality of the agricultural products often reduce because their own perishable and anthropogenic factors in the process from the supplier via the retailer to final consumers. This paper takes shortage and deterioration factors into account, establishes related profit functions of the supplier and the retailer, obtains the optimal ordering cycle and quantity decisions, and analyzes the impact of deterioration on decision makers under the decentralized system. To maximize the total profit of supply chain, we explore coordination mechanisms for the supply chain when considering shortage and deterioration factors, such as revenue sharing contract, quantity discount contract and buy-back contract. By comparing these different contracts, the coordination effect is analyzed in details. Finally, through series of numerical analysis, some managerial insights are provided.

Synthesis of Porous Carbon Supported Palladium Nanoparticle Catalysts by Atomic Layer Deposition: Application for Rechargeable Lithium–O₂ Battery

Lei, Yu,Lu, Jun,Luo, Xiangyi,Wu, Tianpin,Du, Peng,Zhang, Xiaoyi,Ren, Yang,Wen, Jianguo,Miller, Dean J.,Miller, Jeffrey T.,Sun, Yang-Kook,Elam, Jeffrey W.,Amine, Khalil American Chemical Society 2013 Nano letters Vol.13 No.9

<P>In this study, atomic layer deposition (ALD) was used to deposit nanostructured palladium on porous carbon as the cathode material for Li–O<SUB>2</SUB> cells. Scanning transmission electron microscopy showed discrete crystalline nanoparticles decorating the surface of the porous carbon support, where the size could be controlled in the range of 2–8 nm and depended on the number of Pd ALD cycles performed. X-ray absorption spectroscopy at the Pd K-edge revealed that the carbon supported Pd existed in a mixed phase of metallic palladium and palladium oxide. The conformality of ALD allowed us to uniformly disperse the Pd catalyst onto the carbon support while preserving the initial porous structure. As a result, the charging and discharging performance of the oxygen cathode in a Li–O<SUB>2</SUB> cell was improved. Our results suggest that ALD is a promising technique for tailoring the surface composition and structure of nanoporous supports in energy storage devices.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2013/nalefd.2013.13.issue-9/nl401833p/production/images/medium/nl-2013-01833p_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl401833p'>ACS Electronic Supporting Info</A></P>

Implementation of One-Cycle Control for Switched Capacitor Converters

Lei Yang,Xiaobin Zhang,Guann-pyng Li 전력전자학회 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.6

An extension of the one-cycle control (OCC) method for switched-capacitor (SC) converters is proposed in this paper, featuring a fast dynamic response, wide line and load operation ranges, and simplicity in implementation. To illustrate the operation principle of this nonlinear control method and to demonstrate its simplicity in design, a dual-phase unity gain SC converter is examined. A new control loop based on the charge balance in a flying capacitor is formulated for the OCC technique and implemented with a 15W dual-phase unity gain SC converter on a circuit board for control verification. The obtained experimental results show that external disturbances can be rejected in one switching cycle by the OCC controlled SC converter with good line and load regulations. When compared to other control methods, the proposed nonlinear control loop exhibits superior dynamic performance in suppressing input and load disturbances.