The USP21/YY1/SNHG16 axis contributes to tumor proliferation, migration, and invasion of non-small-cell lung cancer

Pei Xu,Haibo Xiao,Qi Yang,Rui Hu,Lianyong Jiang,Rui Bi,Xueyan Jiang,Lei Wang,Ju Mei,Fangbao Ding,Jianbing Huang 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

Deubiquitinases (DUBs) and noncoding RNAs have been the subjects of recent extensive studies regarding their roles in lung cancer, but the mechanisms involved are largely unknown. In our study, we used The Cancer Genome Atlas data set and bioinformatics analyses and identified USP21, a DUB, as a potential contributor to oncogenesis in nonsmall-cell lung cancer (NSCLC). We further demonstrated that USP21 was highly expressed in NSCLCs. We then conducted a series of in vitro and in vivo assays to explore the effect of USP21 on NSCLC progression and the underlying mechanism involved. USP21 promoted NSCLC cell proliferation, migration, and invasion and in vivo tumor growth by stabilizing a well-known oncogene, Yin Yang-1 (YY1), via mediating its deubiquitination. Furthermore, YY1 transcriptionally regulates the expression of SNHG16. Moreover, StarBase bioinformatics analyses predicted that miR4500 targets SNHG16 and USP21. A series of in vitro experiments indicated that SNHG16 increased the expression of USP21 through miR-4500. In summary, the USP21/YY1/SNHG16 axis plays a role in promoting the progression of NSCLC. Therefore, the USP21/YY1/SNHG16/miR-4500 axis may be a potential therapeutic target in NSCLC treatment.

Improved Work Function of Preferentially Oriented Indium Oxide Films Induced by the Plasma Exposure Technique

Lei Yang,Shuai Guo,Qiuling Yang,Yuankun Zhu,Bing Dai,Hailing Yu,Pei Lei,Jiecai Han,Ying Hou,Jiaqi Zhu 대한금속·재료학회 2015 ELECTRONIC MATERIALS LETTERS Vol.11 No.6

The preferentially oriented In2O3 thin films were prepared on glass substrates by conventional magnetron sputtering with Ar+ plasma exposure at room temperature. Based on the x-ray diffraction, x-ray photoelectron spectroscopy, and UV photoelectron spectroscopy results, it was found that the Ar+ plasma exposure not only enhanced the low-temperature crystallization of In2O3 thin films, but also led to a dramatic improvement in the work function. Furthermore, it demonstrated that the shift mechanism of the work function in In2O3 thin films mainly combined with theelimination of oxygen defects and the change of the preferential orientation of In2O3 film surface.

Analysis and Design of Low Pass Filter using Unit Cell based on CRLH Transmission Line

Yang, Lei,Yang, Doo-Yeong The Korea Contents Association 2012 International Journal of Contents Vol.8 No.3

In this paper, a unit cell for low pass filter design by using composite right and left-handed transmission line in conductor-backed coplanar waveguide is proposed. The characteristics of the unit cell are analyzed in order to design a low pass filter in small sizes. By changing the sizes of the unit cell, the parameters of right-handed and left-handed immittance components are changed and the desired characteristics of the unit cell are achieved. The equivalent circuit of the unit cell is extracted and analyzed either. As a result, the simulation results of the unit cell and the equivalent circuit are almost identified. The movement and energy distributions of electromagnetic field are shown to confirm the property of the unit cell. In the end, a low pass filter is demonstrated by cascading three proposed unit cells, which shows cutoff frequency of 1.53GHz and deep attenuation from 2.23GHz to 4.49GHz lower than -50dB.

KDM3A promotes oral squamous cell carcinoma cell proliferation and invasion via H3K9me2 demethylation-activated DCLK1

Yang Lei,Zhang Qiqiong,Yang Qiuye 한국유전학회 2022 Genes & Genomics Vol.44 No.11

Background: Oral squamous cell carcinoma (OSCC) is a frequently-diagnosed malignancy with high potential for proliferation and invasion. Histone methylation is known as a crucial mechanism that regulates pathological processes in various cancers, including OSCC. Objective: This study sought to delve into the molecular mechanism of lysine demethylase 3 A (KDM3A) in OSCC cell proliferation and invasion. Methods: Expression levels of KDM3A, lysin-9 of di-methylated histone H3 (H3K9me2), and doublecortin-like kinase 1 (DCLK1) in cells were determined by reverse-transcription quantitative polymerase chain reaction or Western blot analysis. Cell proliferation and invasion were evaluated by cell counting kit-8, colony formation, and Transwell assays. The enrichment of KDM3A and H3K9me2 on the DCLK1 promoter was determined by chromatin immunoprecipitation assay. The functional rescue experiment was performed with DCLK1 overexpression vector and si-KDM3A in CAL-27 and SCC-9 cells. Results: KDM3A was elevated in OSCC cells. KDM3A knockdown suppressed OSCC proliferation and invasion, along with increased H3K9me2 level in OSCC cells. KDM3A and H3K9me2 were enriched on the DCLK1 promoter and inhibiting H3K9me2 improved DCLK1 expression levels. DCLK1 overexpression neutralized the inhibition of KDM3A knockdown on OSCC proliferation and invasion. Conclusions: KDM3A facilitated OSCC proliferation and invasion by eliminating H3K9me2 to upregulate DCLK1 expression levels.

Implementation of One-Cycle Control for Switched Capacitor Converters

Lei Yang,Xiaobin Zhang,Guann-pyng Li 전력전자학회 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.6

An extension of the one-cycle control (OCC) method for switched-capacitor (SC) converters is proposed in this paper, featuring a fast dynamic response, wide line and load operation ranges, and simplicity in implementation. To illustrate the operation principle of this nonlinear control method and to demonstrate its simplicity in design, a dual-phase unity gain SC converter is examined. A new control loop based on the charge balance in a flying capacitor is formulated for the OCC technique and implemented with a 15W dual-phase unity gain SC converter on a circuit board for control verification. The obtained experimental results show that external disturbances can be rejected in one switching cycle by the OCC controlled SC converter with good line and load regulations. When compared to other control methods, the proposed nonlinear control loop exhibits superior dynamic performance in suppressing input and load disturbances.

Synthesis of Porous Carbon Supported Palladium Nanoparticle Catalysts by Atomic Layer Deposition: Application for Rechargeable Lithium–O₂ Battery

Lei, Yu,Lu, Jun,Luo, Xiangyi,Wu, Tianpin,Du, Peng,Zhang, Xiaoyi,Ren, Yang,Wen, Jianguo,Miller, Dean J.,Miller, Jeffrey T.,Sun, Yang-Kook,Elam, Jeffrey W.,Amine, Khalil American Chemical Society 2013 Nano letters Vol.13 No.9

<P>In this study, atomic layer deposition (ALD) was used to deposit nanostructured palladium on porous carbon as the cathode material for Li–O<SUB>2</SUB> cells. Scanning transmission electron microscopy showed discrete crystalline nanoparticles decorating the surface of the porous carbon support, where the size could be controlled in the range of 2–8 nm and depended on the number of Pd ALD cycles performed. X-ray absorption spectroscopy at the Pd K-edge revealed that the carbon supported Pd existed in a mixed phase of metallic palladium and palladium oxide. The conformality of ALD allowed us to uniformly disperse the Pd catalyst onto the carbon support while preserving the initial porous structure. As a result, the charging and discharging performance of the oxygen cathode in a Li–O<SUB>2</SUB> cell was improved. Our results suggest that ALD is a promising technique for tailoring the surface composition and structure of nanoporous supports in energy storage devices.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2013/nalefd.2013.13.issue-9/nl401833p/production/images/medium/nl-2013-01833p_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl401833p'>ACS Electronic Supporting Info</A></P>

Improvement of comprehensive performance of compound green soil in sponge city

Lei Zhang,Zhicheng Li,Tianliang Yang,Ping Yang 대한환경공학회 2021 Environmental Engineering Research Vol.26 No.5

Large-scale constructions of urbanization increase the impervious areas of city, leading to the urban hydrological effects such as urban waterlogging and rainwater runoff pollution. To this end, China proposed to adopt the measure to build sponge cities. However, the existing green soil cannot meet the comprehensive needs of sponge city. In order to quickly evaluate the comprehensive performance of the soil in sponge city, a comprehensive evaluation criterion was designed, which is related to the characteristics of greening soil permeability, porosity, pH, salinity and fertility. Based on the criterion, a new type of composite green soil was obtained with the silt soil: medium sand: sawdust ratio of 72.5%: 20%: 7.5%. Finally, compared with the existing soil, the new green soil not only meets the comprehensive performance requirements of the green soil’s fertility, pH, permeability and other factors, but also has higher permeability and water retention. It was proved that the new green soil has apparent advantages in the control of rainwater.

A Hybrid Static Compensator for Dynamic Reactive Power Compensation and Harmonic Suppression

Yang, Jia-qiang,Yang, Lei,Su, Zi-peng The Korean Institute of Power Electronics 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.3

This paper presents a combined system of a small-capacity inverter and multigroup delta-connected thyristor switched capacitors (TSCs). The system is referred to as a hybrid static compensator (HSC) and has the functions of dynamic reactive power compensation and harmonic suppression. In the proposed topology, the load reactive power is mainly compensated by the TSCs. Meanwhile the inverter is meant to cooperate with TSCs to achieve continuous reactive power compensation, and to filter the harmonics generated by nonlinear loads and the TSCs. First, the structure and mathematical model of the HSC are discussed Then the control method of the HSC is presented. An improved reduced order generalized integrator (ROGI)-based selective current control method is adopted in the inverter to achieve high-performance reactive and harmonic current compensation. Meanwhile, a switch control strategy is proposed to implement precise and fast switching of the TSCs and to avoid changing the time delay needed by the conventional switch strategy. Experiments are implemented on a 20 KVA HSC prototype and the obtained results verify the validity of the proposed HSC system.

Estimation of Dynamic Behaviors of Bedrock Foundation Subjected to Seismic Loads Based on FEM and DEM Simulations

Lei Yang,Yujing Jiang,Bo Li,Shucai Li,Gang Wang 대한토목학회 2013 KSCE JOURNAL OF CIVIL ENGINEERING Vol.17 No.2

Seismic behavior of bedrock foundation remains as one of the most fundamental and important problems concerning the stability and safety of nuclear power plants. The dynamic FEM (Finite Element Method) is commonly utilized in analyzing the seismic responses of bedrock; while in recent decades, the DEM (Distinct Element Method) has attracted more and more attentions, which has a better capability of simulating the sliding and separation of discontinuities in dynamic simulations. In this study, the FEM and DEM were adopted to investigate the seismic behavior of the bedrock of a nuclear power plant located in Japan, and the differences between the two methods in dynamic simulations were illuminated. Simulation results using FEM and DEM models without discontinuities agree well with each other, exhibiting an amplification effect of intact bedrock on the seismic wave propagation. Numerical simulation results obtained from the models containing faults give similar responses of bedrock to the input seismic waves; however, the FEM model underestimates the weakening effect of discontinuities on the propagation of seismic waves due to that it cannot represent well the large deformational behavior of discontinuities. When large deformation happens due to large seismic loads, the DEM can be regarded as a better method in seismic response evaluations of bedrock with discontinuities.

New insights into sulfur poisoning behavior of Ni-YSZ anode from long-term operation of anode-supported SOFCs

Yang, Lei,Cheng, Zhe,Liu, Meilin,Wilson, Lane Royal Society of Chemistry 2010 ENERGY AND ENVIRONMENTAL SCIENCE Vol.3 No.11

<P>The performance characteristics of high-performance Ni-YSZ (yttria-stabilized zirconia) anode-supported solid oxide fuel cells (SOFCs) are examined under typical fuel cell operating conditions over a period of ∼3000 h when hydrogen contaminated with different concentrations of H<SUB>2</SUB>S was used as the fuel. Analyses reveal some new insights into the sulfur poisoning behavior of a Ni-YSZ anode. First, an initial drop in power output upon exposure to H<SUB>2</SUB>S-contaminated fuels is more dramatic in an anode-supported cell (with small cell resistance) than in electrolyte-supported cell (with large cell resistance), creating an illusion that a high-performance cell appears to be less sulfur tolerant than a low-performance cell. Second, the transition time for this rapid performance drop to cease is much longer for an anode-supported cell (with a thicker anode) than an electrolyte-supported cell (with a thinner anode). Third, the degree of sulfur poisoning (or the cell resistance increase due to sulfur poisoning) diminishes with operating cell current density, suggesting that the water produced at the active sites on anode surface from electro-oxidation of hydrogen (or oxygen ions) may promote the oxidation and removal of adsorbed sulfur. This effect becomes less pronounced at higher concentration of H<SUB>2</SUB>S, but still significant at ∼1 ppm of H<SUB>2</SUB>S. Fourth, the subsequent slow degradation in performance after the rapid performance drop upon initial exposure to H<SUB>2</SUB>S can be avoided, indicating that the previously reported slow degradation is unlikely the inherent behavior of a Ni-YSZ anode, but associated with other complications. This further implies that sulfur poisoning may be reversible and the performance drop could be fully recovered when the fuel is switched back to clean hydrogen.</P> <P>Graphic Abstract</P><P>While the initial drop in performance of a Ni-YSZ anode is sensitive to miniscule concentration of H<SUB>2</SUB>S, the subsequent performance is stable for ∼3000 h and the degree of sulfur poisoning diminishes with cell current density. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0ee00386g'> </P>