Paraquat 중독환자의 초기검사로서 sodium dithionite를 이용한 소변내 paraquat검출의 임상적 의의

윤갑준,임경수,이진웅,김영식,이부수,박덕우,김선만,이강현,황성오,안무업 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Background : Mortality from paraquat intoxication depends upon plasma paraquat concentration. To know the severity of paraquat intoxication is important for directing therapeutic modality and predicting prognosis. Sodium dithionite test for urinary paraquat provides an easy and simple method to determine the severity of paraquat intoxication in emergency department. purpose : To determine whether the result urinary paraquat test by sodium dithionite can predict outcome in patients with paraquat intoxication in emergency department. Subjects : 48 patients(male 31, female 17, mean age 37 years) who had exposure to paraquat and presented within 24 hours after exposure. Result : Thirty five patients were positive in paraquat urine test and thirteen patients were negative. Clinical manifestations were more severe in positive patients than in negatives. Complication was much more in positives than in negatives. 28 of 35 patients(80%) in positives and 2 of 13 patients(15%) in negatives died. Conclusion : Positive test for urinary paraquat is associated with high mortality and morbidity from paraquat intoxication, and qualitative test for urinary paraquat by sodium dithionite is an useful method to determine the severity of paraquat intoxication in emergency department.

응급센터내에서의 Do-Not-Resuscitate Order

김영식,황성오,이부수,안무업,임경수,강성준 대한응급의학회 1993 대한응급의학회지 Vol.4 No.2

Do-not-resuscitate(DNR) decision in certain patients is a important part of patient management. The use of DNR order has been widely recognized in hospitalized patients, but there has been little discussion of the use of DNR order in emergency department. DNR decision in emergency department is difficult because there is no previous contact between physician and patient. To identify the medical reasons of the DNR decision and the process of the DNR in the emergency department, 293 DNR patients in emergency department during 1 year and 36 physicians having experienced DNR decision were studied. Reasons for DNR are irreversible states from brain damage or acute illness(63%), chronic irreversible illness(15%), prolonged cardiac arrest over 30 minutes(16%), physical injuries including decapitation(1%) and family's hope(5%). Almost DNR(72%) were decided by physicians. 72% of DNR decision were not documented. Cardiopulmonary resuscitation was attempted before DNR decision in 38% of the patients and 17% of CPR attempts were unnecessary. Almost physician(88%) replied that physician should discuss resuscitation with family and process of DNR decision should be documented.

Cynara scolymus L. Inhibits the LPS-induced Inflammatory Reaction via Suppression of NF-κB Activity in RAW 264.7 Cells

Kyung-Jun Boo(Kyung-Jun Boo),Kiman Lee(Kiman Lee),Il-Ho Park(Il-Ho Park),Tae Jin Kang(Tae Jin Kang) 대한약학회 2023 약학회지 Vol.67 No.1

Cynara scolymus L. (artichoke) has been traditionally used in the treatment of digestive-related disease, severe hyperlipidemia and liver disease. Recently, anti-inflammatory effect of artichoke has been reported by several studies, but its mechanisms are still unclear. In this study, the anti-inflammatory mechanism of artichoke was studied using an in vitro acute inflammation model. The effect of artichoke on the expression of pro-inflammatory cytokines, such as interleukin- 1beta (IL-1β), IL-6, and tumor necrosis factor-alpha (TNF-α) in murine macrophage cell line, RAW264.7, was examined by using ELISA and RT-PCR. As a result, artichoke inhibited the expression of IL-1β, IL-6 and TNF-α in macrophages activated by lipopolysaccharide (LPS) at a dose dependent manner. The expression of inflammatory mediators such as NO and PGE2 was next investigated in RAW264.7 cells stimulated with LPS. Artichoke also inhibited the production of NO and PGE2 in the cells at a dose dependent manner. Artichoke suppressed the mRNA expression of iNOS and COX-2 in macrophages by LPS. The effect of artichoke on the activation of NF-κB was examined and LPS-induced NF-κB activation was decreased by treatment of artichoke, suggesting that artichoke has anti-inflammatory effect by inhibiting NF- κB activation.

Hycanthone Inhibits Inflammasome Activation and Neuroinflammation-Induced Depression-Like Behaviors in Mice

( Kyung-jun Boo ),( Edson Luck Gonzales ),( Chilly Gay Remonde ),( Jae Young Seong ),( Se Jin Jeon ),( Yeong-min Park ),( Byung-joo Ham ),( Chan Young Shin ) 한국응용약물학회 2023 Biomolecules & Therapeutics(구 응용약물학회지) Vol.31 No.2

Despite the various medications used in clinics, the efforts to develop more effective treatments for depression continue to increase in the past decades mainly because of the treatment-resistant population, and the testing of several hypotheses- and target-based treatments. Undesirable side effects and unresponsiveness to current medications fuel the drive to solve this top global health problem. In this study, we focused on neuroinflammatory response-mediated depression which represents a cluster of depression etiology both in animal models and humans. Several meta-analyses reported that proinflammatory cytokines such as interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α) were increased in major depressive disorder patients. Inflammatory mediators implicated in depression include type-I interferon and inflammasome pathways. To elucidate the molecular mechanisms of neuroinflammatory cascades underlying the pathophysiology of depression, we introduced hycanthone, an antischistosomal drug, to check whether it can counteract depressive-like behaviors in vivo and normalize the inflammation-induced changes in vitro. Lipopolysaccharide (LPS) treatment increased proinflammatory cytokine expression in the murine microglial cells as well as the stimulation of type I interferon-related pathways that are directly or indirectly regulated by Janus kinase-signal transducer and activator of transcription (JAK-STAT) activation. Hycanthone treatment attenuated those changes possibly by inhibiting the JAK-STAT pathway and inflammasome activation. Hycanthone also ameliorated depressive-like behaviors by LPS. Taken together, we suggest that the inhibitory action of hycanthone against the interferon pathway leading to attenuation of depressive-like behaviors can be a novel therapeutic mechanism for treating depression.

각질세포에서 자외선B가 유도한 세포 사멸, 산화적 스트레스 및 matrix metalloproteinase 1 발현에 대한 죽여추출물의 영향

석진경 ( Jin Kyung Seok ),곽준엽 ( Jun Yup Kwak ),서형호 ( Hyeong Ho Seo ),서화진 ( Hwa Jin Suh ),부용출 ( Yong Chool Boo ) 대한화장품학회 2015 대한화장품학회지 Vol.41 No.1

자외선은 피부 광노화의 주요 요인이며, 효과적인 자외선 차단제가 피부의 건강과 아름다움을 위해 필요하다. 본 연구는 세포 실험을 통하여 자외선에 의해 유도된 세포 사멸, 산화적 스트레스, matrix metalloproteinase 1 발현에 미치는 죽여추출물의 영향을 알아보고자 수행하였다. HaCaT 인간 각질세포를 여러 농도의 죽여추출물 유무 조건에서 자외선을 조사하고 세포의 생존율과 생화학적 과정들의 변화를 분석하였다. 죽여추출물은 자외선을 조사한 세포의 생존율을 증진시켰고, procaspase 3가 활성화 형태로 절단되고, Bax/Bcl-2 비율이 증가하는 세포 자살 과정을 완화시켰다. 죽여추출물은 자외선에 노출된 세포에서 활성산소의 발생과 지질 과산화도 감소시켰다. 또한 죽여추출물은 자외선에 의해 자극된 matrix metalloproteinase 1의 발현과 c-Jun N-terminal kinase의 인산화를 억제하였다. 본 연구는 죽여추출물이 자외선에 의한 세포 사멸, 산화적 스트레스, 그리고 matrix metalloproteinase 1 발현을 억제함을 보여 주었으며, 이 추출물이 피부 광노화의 일부 현상을 억제하는 화장품 원료로 유용함을 시사하였다. Ultraviolet radiation (UV) is a major cause of skin photoaging, and effective UV protecting agents are needed for the skin health and beauty. This study was undertaken to examine the effects of Bambusae caulis in Taeniam extract (BCTE) on UVB-induced cell death, oxidative stress and matrix metalloproteinase 1 (MMP1) expression in cell-based assays. HaCaT human keratinocytes were exposed to UVB in the presence of BCTE at different concentrations and resulting changes in cell viability and biochemical events were determined. The results showed that BCTE enhanced the viabilities of UVB-exposed cells, and attenuated apoptotic events such as cleavage of procaspase 3 to its active form, and the increase of Bax to Bcl-2 ratios. BCTE also attenuated the reactive oxygen generation and lipid peroxidation in cells exposed to UVB. Additionally, it attenuated the expression of matrix metalloproteinase 1 and the phosphorylation of c-Jun N-terminal kinase stimulated by UVB. Conclusively, the present study demonstrated that BCTE pro tected skin cells from the UVB-induced cell death, oxidative stress and MMP1 expression, suggesting its potential use as a cosmetic ingredient mitigating some features of the skin photoaging.

Ginsenoside Production and Morphological Characterization of Wild Ginseng (Panax ginseng Meyer) Mutant Lines Induced by γ-irradiation (⁶⁰Co) of Adventitious Roots

Jun-Ying Zhang,Tae-Woong Bae,Kyung-Hwan Boo,Hyeon-Jin Sun,In-Ja Song,Chi-Hoa Pham,Markkandan Ganesan,Dae-Hwa Yang,Hong-Gyu Kang,Suk-Min Ko,Key-Zung Riu,Pyung-Ok Lim,Hyo-Yeon Lee 고려인삼학회 2011 Journal of Ginseng Research Vol.35 No.3

With the purpose of improving ginsenoside content in adventitious root cultures of Korean wild ginseng (Panax ginseng Meyer), the roots were treated with different dosages of γ-ray (5, 10, 25, 50, 75, 100, and 200 Gy). The growth of adventitious roots was inhibited at over 100 Gy. The irradiated adventitious roots showed significant variation in the morphological parameters and crude saponin content at 50 to100 Gy. Therefore, four mutant cell lines out of the propagation of 35 cell lines treated with 50 Gy and 100 Gy were selected on the basis of phenotypic morphology and crude saponin contents relative to the wild type control. The contents of 7 major ginsenosides (Rg₁, Re, Rb₁, Rb₂, Rc, Rf, and Rd) were determined for cell lines 1 and 3 from 100 Gy and lines 2 and 4 from 50 Gy treatments. Cell line 2 showed more secondary roots, longer length and superior growth rate than the root controls in flasks and bioreactors. Cell line 1 showed larger average diameter and the growth rate in the bioreactor was comparable with that of the control but greater in the flask cultured roots. Cell lines 1 and 2, especially the former, showed much more ginsenoside contents than the control in flasks and bioreactors. Therefore, we chose cell line 1 for further study of ginsenoside contents. The crude saponin content of line 1 in flask and bioreactor cultures increased by 1.4 and 1.8-fold, respectively, compared to the control. Total contents of 7 ginsenoside types (Rg₁, Re, Rb₁, Rb₂, Rc, Rf, and Rd) increased by 1.8 and 2.3-fold, respectively compared to the control. Crude saponin and ginsenoside contents in the bioreactor culture increased by about 1.4-fold compared to that the flask culture.

Ginsenoside Production and Morphological Characterization of Wild Ginseng (Panax ginseng Meyer) Mutant Lines Induced by g-irradiation (^(60)Co) of Adventitious Roots

Jun-Ying Zhang,배태웅,Kyung-Hwan Boo,선현진,송인자,Chi-Hoa Pham,Markkandan Ganesan,양대화,강홍규,Suk-Min Ko,Key-Zung Riu,Pyung-Ok Lim,Hyo-Yeon Lee 고려인삼학회 2011 Journal of Ginseng Research Vol.35 No.3

With the purpose of improving ginsenoside content in adventitious root cultures of Korean wild ginseng (Panax ginseng Meyer),the roots were treated with different dosages of γ-ray (5, 10, 25, 50, 75, 100, and 200 Gy). The growth of adventitious roots was inhibited at over 100 Gy. The irradiated adventitious roots showed significant variation in the morphological parameters and crude saponin content at 50 to100 Gy. Therefore, four mutant cell lines out of the propagation of 35 cell lines treated with 50 Gy and 100Gy were selected on the basis of phenotypic morphology and crude saponin contents relative to the wild type control. The contents of 7 major ginsenosides (Rg_1, Re, Rb_1, Rb_2, Rc, Rf, and Rd) were determined for cell lines 1 and 3 from 100 Gy and lines 2 and 4from 50 Gy treatments. Cell line 2 showed more secondary roots, longer length and superior growth rate than the root controls in flasks and bioreactors. Cell line 1 showed larger average diameter and the growth rate in the bioreactor was comparable with that of the control but greater in the flask cultured roots. Cell lines 1 and 2, especially the former, showed much more ginsenoside contents than the control in flasks and bioreactors. Therefore, we chose cell line 1 for further study of ginsenoside contents. The crude saponin content of line 1 in flask and bioreactor cultures increased by 1.4 and 1.8-fold, respectively, compared to the control. Total contents of 7 ginsenoside types (Rg_1, Re, Rb_1, Rb_2, Rc, Rf, and Rd) increased by 1.8 and 2.3-fold, respectively compared to the control. Crude saponin and ginsenoside contents in the bioreactor culture increased by about 1.4-fold compared to that the flask culture.

P137 : A study of the effects of physical dermabrasion combined with chemical peeling

( Boo Kyoung Kang ),( Tae In Kim ),( Myong Il Bae ),( Sung Hyuk Moon ),( Ki Heon Jeong ),( Jong Min Park ),( Dong Hye Suh ),( Sang Jun Lee ),( Min Kyung Shin ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: Many comparative studies of chemical peeling and dermabrasion have been reported. However, no basic scientific data about the immediate effects after combined treatment of chemical peels and dermabrasion have been confirmed. Objectives: To evaluate the effect of the application of physical abrasion in combination with chemical peels. Methods: Three porcine were treated with physical abrasion using a water jet device in combination with an α-hydroxy acid solution, and the controlled skin samples received chemical peeling solution alone. The levels of growth factors and neuropeptides were measured with a multiplex immunoassay. Results: Skin treated with physical dermabrasion combined with chemical peeling showed prominent detachment and swelling of the stratum corneum, and fluid collection in the hair follicles. The mean cell count of CD34 positive fibroblasts and mast cells, levels of epidermal growth factor, fibroblast growth factor-2, vascular endothelial growth factor and neurotensin were significantly increased in the tissue treated with physical assault combined with a chemical peeling agent compared to the controlled skin. Conclusion: We concluded that physical dermabrasion combined with chemical peeling can be more effective than chemical peeling for the approach through the hair follicle.

Novel Disease Model of Chronic Neutrophilic Leukemia

Jun Hong Park,Kil Soo Kim,Eun Ju Lee,Myoung Ok Kim,Sung Hyun Kim,Kyoungin-Cho,Boo Kyung Jung,Hee Chul Kim,Sol ha Hwang,Zae Young Ryoo 한국실험동물학회 2003 한국실험동물학회 학술발표대회 논문집 Vol.2003 No.-

Production of the Novel Disease Animal Model by Used Tet-off System

Jun Hong Park,Kil Soo Kim,Eun Ju Lee,Myoung Ok Kim,Sung Hyun Kim,Kyoungin-Cho,Boo Kyung Jung,Hee Chul Kim,Sol ha Hwang,Zae Young Ryoo 한국동물생명공학회(구 한국동물번식학회) 2003 Reproductive & developmental biology Vol.27 No.1