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강홍규,김태성,박소현,김태완,유성영 한국환경생물학회 2016 환경생물 : 환경생물학회지 Vol.34 No.4
The objective of this study was to determine the growth and light utilization efficiency of garden plants in shade area through chlorophyll fluorescence reaction analysis. Ten garden plants was grown for 75 days under 50% and 80% shading conditions. Under shading, ET2O/RC, the fluorescence parameter related to electron-transport in photosystem II, was effectively enhanced. However, the electron transport flux until PSI acceptors per reaction center (RE1O/RC) was reduced. These changes in photochemical parameters evoked a decrease in performance index (PI) and driving force (DF) of electron transport flux. In addition, some photochemical parameters such as FV, FV/FO, RE1O/RC, ET2O/RC, PITOTAL ABS, and DFTOTAL ABS were found to be important for shade tolerance. Three species (Pachysandra terminalis Siebold & Zucc, Physostegia virginiana L., and Carex maculata Bott) were found to be shade tolerant. Based on these results, shading factor index (SFI) deduced from photochemical parameters is useful for evaluating of shading stress of garden plants.
Pollen-mediated flow of bar gene in transgenic herbicide-resistant turf grass Zoysia japonica
강홍규,정옥철,배태웅,선현진,송인자,박기웅,임평옥,이재천,이용억,송필순,이효연 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.2
Weed control can be most efectively achieved through the use of herbicide-resistance transgene. A preliminary study bar�transgenic Zoysia japonica posed no serious risk on the unintended escape of the transgene from its cultivation site. The present follow-up investigation of the dispersion of pollen and its short-distance escape outside of the bar-transgenic Zoysia japonica habitats were ascertained in terms of environmental factors afecting anthesis and pollen viability. In a 24-h day cycle, zoysiagrass pollen was released predominantly between 08:00 and 10:00, and the pollen was most viable during the same time interval. Optimal temperature and humidity for pollen viability was 15–20 °C and 80–90%, respectively. The pollen germinated in 120 min after anthesis, but under cloudy conditions germination time doubled. No diferences in pol�len viability/longevity between the transgenic and non-transgenic plants were observed. The pollen-mediated gene fow of transgenic Zoysia japonica to wild-type non-transgenic zoysiagrass species was monitored by measuring the cross-over rate of the bar gene in the context of three diferent models. At distances within 5 m, the rate of gene fow ranged from 3 to 5.7% according to the models used. The greater the distance from the transgenic plant site, the lower the gene fow rate. The furthest transgene detected was 38 m away and exhibited a 0.25% gene fow rate. The radial model yielded a 3.7% escape rate within a 3 m radius and was wind direction dependent. The distance- and direction-dependent gene fow events were infuenced by wind direction and velocity during fowering season.