Sulforaphane protects against acetaminophen-induced hepatotoxicity

Noh, Jung-Ran,Kim, Yong-Hoon,Hwang, Jung Hwan,Choi, Dong-Hee,Kim, Kyoung-Shim,Oh, Won-Keun,Lee, Chul-Ho Elsevier 2015 Food and chemical toxicology Vol.80 No.-

<P><B>Abstract</B></P> <P>Oxidative stress is closely associated with acetaminophen (APAP)-induced toxicity. Heme oxygenase-1 (HO-1), an antioxidant defense enzyme, has been shown to protect against oxidant-induced tissue injury. This study investigated whether sulforaphane (SFN), as a HO-1 inducer, plays a protective role against APAP hepatotoxicity <I>in vitro</I> and <I>in vivo</I>. Pretreatment of primary hepatocyte with SFN induced nuclear factor E2-factor related factor (Nrf2) target gene expression, especially HO-1 mRNA and protein expression, and suppressed APAP-induced glutathione (GSH) depletion and lipid peroxidation, which eventually leads to hepatocyte cell death. A comparable effect was observed in mice treated with APAP. Mice were treated with 300 mg/kg APAP 30 min after SFN (5 mg/kg) administration and were then sacrificed after 6 h. APAP alone caused severe liver injuries as characterized by increased plasma AST and ALT levels, GSH depletion, apoptosis, and 4-hydroxynonenal (4-HNE) formations. This APAP-induced liver damage was significantly attenuated by pretreatment with SFN. Furthermore, while hepatic reactive oxygen species (ROS) levels were increased by APAP exposure, pretreatment with SFN completely blocked ROS formation. These results suggest that SFN plays a protective role against APAP-mediated hepatotoxicity through antioxidant effects mediated by HO-1 induction. SFN has preventive action in oxidative stress-mediated liver injury.</P> <P><B>Highlights</B></P> <P> <UL> <LI> SFN pretreatment increases the cell viability against APAP-induced toxicity. </LI> <LI> SFN pretreatment protects depletion of cellular GSH after APAP treatment. </LI> <LI> SFN pretreatment enhances Nrf2 target gene expression, especially HO-1 after APAP treatment. </LI> <LI> SFN has protective effect against APAP overdose-induced liver injury <I>in vivo</I> model as well. </LI> </UL> </P>

콘크리트 포장의 하중전달 평가방법연구

심도식,이주형,이종호 도립 강원전문대학 1999 道立 江原專門大學 論文集 Vol.2 No.-

This type of analysis assumes linear elastic material properties and static loading conditions. The well-known ILLI-SLAB finite element program was used for the analysis. Closed form analysis is commonly used to analyze pavement structures. This type of analysis assumes linear elastic material properties and static loading conditions. The well-known ILLI-SLAB finite element program was used for the analysis. Bonded concrete overlay analyzed the stress distribution, behavior and load carrying capacity under track load is made evaluation standard of bonded concrete overlay. The purpose of this dissertation was to develop an evaluation system at joints of jointed concrete pavement using finite element analysis Program, a sensitivity analysis was Performed using ILLI-SLAB program with a selected variables which might affect fairly to on the performance of transverse joints. The most significant variables were selected from precise analysis. It was concluded that the variables which most significantly affect to pavement deflections are the modulus of subgrade reaction(K) and the modulus of dowel/concrete interaction(G), and limiting criteria on the performance of joints at JCP are 300pci, 500,000 lb/in. respectively.

복개터널 역해석에 의한 지반물성값 추정에 관한 연구

심도식,박병수,이종호 도립 강원전문대학 1999 道立 江原專門大學 論文集 Vol.2 No.-

This paper is a research about back analysis which optimizes uncertain geotechnical properties from the data measured during geotechnical design and construction. Various numerical optimization algorithms were applied to the excavation problem to determine which method showed the best results with respect to robustness of success in finding an optimal solution to within a certain accuracy and number of function evaluations. The results of back analysis of elastic modulus and Poison's ratio are agree with exact values with a little difference. Therefore, it is known that this study can be efficiency applied for estimating unknown parameters.

국산 침엽수 철물접합부의 인장하중 특성

심국보,박정환,이준호 한국목재공학회 2003 목재공학 Vol.31 No.5

국산 주요 침엽수재인 소나무, 잣나무, 낙엽송의 철물접합부 인장하중 특성 구명을 위하여 본 연구를 수행하였다. 철물 접합부의 최대 인장하중은 AA형의 경우 소나무가 3,612 kgf로 낙엽송, 잣나무에 비해 각각 1.2배, 1.7배, EA형의 경우 2,704 k9f로 1.1배, 1.5배 우수하였다. 또한 AA형은 EA형에 비해 3 수종 모두 약 1.2배 이상 높은 인장하중을 나타내었다. 철물접합부의 파괴모드는 주로 이의 빠짐, 철물의 인장파괴와 목재의 전단파괴로 이루어지며, 특히 소나무 접합부는 철물의 인장파괴를 나타내었다. 철물접합부재의 인장하중-변위 특성은 Foschi 모형으로 정확하게 추정할 수 있었으며 , 철물접합부의 초기강성은 소나무 접합부재가 높게 나타났다. 국산 침엽수재의 철물접합부에 대한 설계하중은 소나무, 낙엽송, 잣나무의 순으로 높게 나타났으며, AA형의 경우 이당 설계하중이 소나무 25 kgf, 낙엽송 22 kgf, 잣나무 15 kgf이었으며, EA형의 경우 소나무 19 kgf, 낙엽송 17 kgf, 잣나무 13 kgf 이었다. This study was conducted to evaluate the tensile properties of metal plate connector for the domestic major softwoods, such as Korean red pine, Korean white pine, and Japanese larch. The maximum tensile load of Korean red pine was 3,612㎏f in AA type, it was 1.2 and 1.7 times higher load than that of Japanese larch and Korean white pine. In EA type, it was 2,704㎏f, and 1.1 and 1.5 times higher than the loads of Japanese larch and Korean white pine. The failure modes of metal plate connector were metal plate withdrawal, plate tensile failure, and wood shear block failure. The failure mode of Korean red pine connector was tensile failure of plate, that is reason of the high tensile load resistance for metal plate connections in Korean red pine. The mechanical properties of metal plate connector could be predicted by the Foschi model parameter. In the initial stage, the Korean red pine connector was stiffer than the other species. The design values for metal plate connector per tooth was 25, 22, and 15kgf for Korean red pine, Japanese larch, and Korean white pine in AA type and 19, 17, and 13㎏f in EA type.

수돗물에서 동 배관의 부식특성에 관한 연구

심재주,신동호,최윤석,김정구,유승재 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.12

The corrosion behavior of copper in neutral aerated synthetic tap water was investigated using electrochemical methods, SEM and XPS. All potentiodynamic polarization curves showed active corrosion behavior, and the corrosion rate was more sensitive to flow velocity than Cl^(-) ion concentration and temperature. The result of potentiostatic test indicated that the current density decreased with increasing time regardless of Cl^(-) ion concentration and temperature in stagnant condition because copper compounds were formed uniformly on the surface. The EIS plot was changed from two time constants to three time constants with the formation of Cu₂O, and the charge transfer resistance (R_(ct)) was very large and increased with increasing immersion time. From all results, copper had good corrosion resistance in this potable water system.

희생양극을 이용한 자동차 소음기의 음극방식에 관한 연구

신동호,심재주,최윤석,김정구 대한금속재료학회 2004 대한금속·재료학회지 Vol.42 No.12

In order to apply a sacrificial anode cathodic protection system for automotive muffler materials, determination of reliable sacrificial anode and its protection ability were evaluated by laboratory tests in synthetic condensed water. Three kinds of sacrificial anodes were selected: aluminum (AI), zinc (Zn) and magnesium (Mg), and the muffler materials were Al-coated steel and uncoated steel. The corrosion behavior in synthetic condensed water for the muffler materials and sacrificial anodes was investigated by potentiodynamic tests. The cathodic protection ability was evaluated by galvanic corrosion test and weight-loss test. The results of the potentiodynamic test indicated that Al was less suitable for sacrificial anode because it became passive. Mg was also unqualified due to the high dissolution rate. However, Zn was adequate for sacrificial anode because of its low dissolution rate, high stability and suitable potential difference. Results of weight-loss test demonstrated that the corrosion rate of Al-coated steel was reduced from 0.028 to 0.018 mm/year, and that of uncoated steel was 0.08 to 0.002 mm/year by coupling with Zn anode. In addition, the proper area ratio of anode to cathode was 1 : 10. From these results, it can be concluded that Zn anode can protect both the Al-coating with passivation, and steel substrate with low dissolution rate. (Received January 8, 2004)

WDM 시스템용 광대역 증폭기의 시뮬레이션

李鎬俊,沈雲用,鄭白鎬 湖西大學校工業技術硏究所 1999 工業技術硏究所論文集 Vol.18 No.-

In recent years, the biggest issue of WDM system is in increasing its bandwidth. Transmission bandwidth of WDM may be limited by many kinds of component, and the bandwidth plays impotent role in most of all optical amplifiers. Transmission bandwidth of recent WDM is limited in about 40 nm. This limit depends on EDFA(?? Fiber Amplifier) gain bandwidth. To overcome this problem, we may use L and S band using Yb/Nd doped fiber amplifier. Wideband amplifier, which is consisted of RFA for L band and EDFA for M band using single pump laser diode, is presented. The amplifiers have been analyzed numerically for the design.

상완골 과 상부 골절에서의 이중 긴장 대 기법을 이용한 수술적 치료

강호정,김홍희,심동준,한수봉,강응식 대한골절학회 2002 대한골절학회지 Vol.15 No.4

목 적 : 이중 긴장 대 기법을 이용한 상완골 과 상부 골절의 임상적 결과를 분석하여 그 유용성을 보고하고자 한다. 대상 및 방법 : 1992년 10월부터 2001년 9월까지 상완골 원 위부 골절로 치료한 환자 중 이중 긴장 대 기법을 시행한 14예를 대상으로 하였다. 골절의 원인으로서는 실족에 의한 단순 손상이 11예, 교통사고에 의한 손상이 2예, 직접 타격에 의한 손상이 1예가 있었다. AO/ASIF 분류 방법에 따라 분류하면 A2가 2예, A3이 1예,. C1이 6예, C2가 3예, C3이 2예였으며, 성별로는 남자가 5예, 여자가 9예였고, 평균 나이는 51.6 세 (최소 15세, 최대 88세)였다. 분쇄 골절이 심한 4예에서 주두 절골 술을 시행하였다. 술 후 고정기간은 평균 20.6일 이었고, 경화를 보인 시기는 술 후 평균 10.1주 였다. 65세 이상의 5예에 있어서는 술 전에 골 밀도 검사를 시행하였으며, 대퇴경부의 평균 T-score가 -3.65였고 요추부의 평균 T-score가 -2.97이였다. 평균 추 시 기간은 평균 16.4개월(최소 12개월, 최대 29개월) 이였다. 평가는 최종 외래 내원시 주관절의 운동 범위, 통증 유무, 환자의 만족도를 조사하였다. 결 과 : 최종 추후 결과를 분석하였으며, 운동범위는 Excellent군이 8예, Good군이 4예, Fair군이 2예 였으며, Poor군은 없었다. 10예에서는 술 후 통증이 전혀 없었으며, 4예에서는 가끔씩 통증을 호소하였다. 합병증으로는 1예에서 주두에 K-강선 삽입부위의 피부자극에 의한 통증으로 술 후 6개월째 조기 제거를 시행하였다. 척골신경 전방 전위 술을 8예에서 시행 하였으며, 시행하지 않은 6예 중에 2예에서 척골신경 자극증상이 있었다. 그 중 1예에서는 술 후 8개월째 척골신경 전방 전위 술을 시행 받았으나, 다른 1예에서는 대중적 치료로 증세가 호전되었다. 1예에서 K-강선 삽입부위에 부분적 피부괴사 소견이 보였으나 핀 제거 술 및 창상 치료 후 치유되었다. 결 론 : 이중 긴장 대 기법을 이용한 상완골 과 상부 골절의 치료가 수술이 간단하고 수술 시간을 단축시킬 수 있으며, 정복 시 골막의 박리 손상을 줄일 수 있어 골편의 탈활(devitalization)이 적으며, 골절부의 압축력을 얻을 수 있다. 특히 젊은 환자의 단순 과 상부 골절에서 과도한 술기를 피하고 조기 관절 운동을 하기위한 경우나, 술 전 골 밀도 검사상 골 다공 증을 동반한 분쇄가 심한 노령 환자의 과상부 및 과간 골절의 치료에 있어 이중 긴장 대 기법은 상완골 상부 골절의 치료에 유용한 방법이 될 수 있으리라 사료된다. PURPOSE : This study evaluated the clinical result of supracondylar fractures of the humans treated with double tension band osteosynthesis. MATERIALS AND METHODS : From October 1992 to September 2001, 14 patients with supracondylar fractures of the humerus were treated by method of double tension band osteosynthesis. eleven fractures were the results of slipped down injury and two traffic accidents and one direct trauma. All fractures were classified according to the AO fracture classification (A2; 2. A3; 1. C1; 6, C2; 3. C3; 2). There were 6 males and 9 females with average age of 51.6 age of 51.6 years (range 15 to 88). Olecranon osteotomy was performed in 4 patients with severe comminuted fractures. Postoperative immobiliza-tions were required for an average of 20.6 days and consolidations were noted after an average of 10.1 weeks. Before operation, bone marrow density studies were performed in five fractures above 65 years. Average T-score of the patients were -3.65 on femur neck and -2.97 on lumbar spine. All patients were re-examined after an average of 16.4 months. RESULTS : Union and consolidation was achieved in all 14 patients judged their results as excellent and four as good. Ten patients had no pain and four had only occasional discomfort. Early removal of K-wires was performed in one patient because irritation of one side K-wires were develop. Sensory change of the ulnar nerve was noted in two patients. Anterior transposition of ulnar nerve was performed in one patient and the other was improved spontaneously. Skin necrosis was developed in one patient but was healed after pin removal and conservative care. CONCLUSION : The advantages of double tension band wiring are an easier and faster procedure, less periosteal and muscle damage, and more symmetrical compression than double plating. Therefore, in the commiuted osteoporotic fracture with poor tolerance for internal fixation with the plate or the simple supracondylar fractures in young age, double tension band osteosynthesis can provide sufficient and secure stability to allow early functional exercise.

후지 사과 Polyphenol Oxidase 의 특성 및 활성억제

최언호,정동선,조남숙,심영현 한국농화학회 1987 Applied Biological Chemistry (Appl Biol Chem) Vol.30 No.3

As a basic research for inhibition of enzymatic browning of apples during dehydration or processing, polyphenol oxidase was extracted from Fuji apples to investigate heat inactivation, chemical inhibition and other properties. Polyphenol oxidase showed the highest activity at 20℃ and pH 5.5 with catechol as substrate, and the Michaelis constant of 0.14 M under the same condition of substrate and pH. The thermal inactivation followed pseudo first-order kinetics to have activation energy of 23.0 k㎈/㏖ and z value of 19.7℃. As for substrate specificity the polyphenol oxidase showed high affinity toward the o-diphenolic compounds, particularly chlorogenic acid. Neither the m- and p-dihydroxy phenols nor monophenols were attacked. Browning by polyphenol oxidase was completely inhibited at the concentrations of l0mM for potassiummetasulfite and thiourea and 1mM for L-cysteine, ascorbic acid and sodium diethyldithiocarbarnate.

Cr, Co, Cu, Ni와 Ca의 첨가에 따른 지하매설배관의 전기화학적 특성에 관한 연구

최윤석,신동호,심재주,김정구 대한금속재료학회 2005 대한금속·재료학회지 Vol.43 No.1

Aqueous corrosion characteristics of low carbon steel with small amounts of Cr, Co, Cu, Ni and Ca, in synthetic groundwater was studied by electrochemical corrosion tests (potentiodynamic test and electrochemical impedance spectroscopy (EIS) measurements) and analytical techniques. Neither carbon steel nor newly alloyed steels showed passive behavior in this synthetic groundwater. Newly alloyed steels, containing Cr-Co, Cr-Cu-Ni and Cr-Cu-Ni-Ca showed higher corrosion resistance than carbon steel in the potentiodynamic tests. EIS measurements showed that the Nyquist plot presented two time constants. The high frequency resistance component (R_(rsut)) and low frequency resistance component (R_(et)) were affected by the alloying elements. The polarization resistance (RP= R_(et) +Ra) of steels could be clearly ranked as Cr-Cu-Ni-Ca steel >> Cr-Cu-Ni steel > Cr-Co steel > carbon steel. Results of surface analyses (XPS and EPMA) showed that Cr and Cu were concentrated in the inner region of rust. However, Co and Ni were uniformly distributed all over the rust layer. These alloying elements improved corrosion resistance of low alloy steel in synthetic groundwater. Especially, Cr-Cu-Ni-Ca steel had the lowest corrosion rate due to the inner rust film formation during the initial stage of corrosion, which suggested a potential for substituting carbon steel in soil environment (Received October 29, 2004)