Regenerative medicine of the Liver: Beyond bench to clinic : Recent Advances and Current Trend in Tissue Engineering

( James J. Yoo ) 대한간학회 2014 춘·추계 학술대회 (KASL) Vol.2014 No.2

Recent advances in tissue engineering and regenerative medicine have provided new therapeutic opportunities for repairing tissue abnormalities. We have followed an engineering strategy that involves the use of biocompatible matrices, either with or without cells. Matrices are either used as a cell delivery vehicle or as scaffolds to promote and enhance tissue regeneration. When cells are used, target cells obtained from donor tissue are expanded in culture, attached to a support matrix, and re-implanted into recipient for recovery of tissue function. While this classical tissue engineering approach using cells and scaffolds has shown to be successful in building new tissues, only a limited number of tissue applications have been translated clinically. It is evident that challenges still exist in developing complex and composite tissue systems that require coordinated and systemic function. In this session, strategies that address these challenges will be discussed.

Regenerative Medicine Strategies for Treating Neurogenic Bladder

James J. Yoo,Jennifer Olson,Anthony Atala,김법완 대한배뇨장애요실금학회 2011 International Neurourology Journal Vol.15 No.3

Neurogenic bladder is a general term encompassing various neurologic dysfunctions of the bladder and the external urethral sphincter. These can be caused by damage or disease. Therapeutic management options can be conservative, minimally invasive, or surgical. The current standard for surgical management is bladder augmentation using intestinal segments. However, because intestinal tissue possesses different functional characteristics than bladder tissue, numerous complications can ensue, including excess mucus production, urinary stone formation, and malignancy. As a result, investigators have sought after alternative solutions. Tissue engineering is a scientific field that uses combinations of cells and biomaterials to encourage regeneration of new, healthy tissue and offers an alternative approach for the replacement of lost or deficient organs, including the bladder. Promising results using tissue-engineered bladder have already been obtained in children with neurogenic bladder caused by myelomeningocele. Human clinical trials, governed by the Food and Drug Administration, are ongoing in the United States in both children and adults to further evaluate the safety and efficacy of this technology. This review will introduce the principles of tissue engineering and discuss how it can be used to treat refractory cases of neurogenic bladder.

Applications of Organoids for Tissue Engineering and Regenerative Medicine

Yoo James J.,조종수,조인호 한국조직공학과 재생의학회 2020 조직공학과 재생의학 Vol.17 No.6

Cell-based Approaches for Treating Erectile Dysfunction

배재현,James J. Yoo 대한남성과학회 2010 The World Journal of Men's Health Vol.28 No.1

Patients with erectile dysfunction (ED) often lose self-esteem, leading to severe psychological impairment. Although many forms of ED can be corrected with currently available therapeutic measures, several types of ED and its associated conditions may not be readily treated. Recently, the concept of cell transplantation has been applied to address ED with the goal of restoring normal anatomical tissue configuration and erectile function. This article provides an overview of the fundamental principles of these cell-based approaches and presents a framework that can be used to interpret current and future studies as well as to encourage further research into cell-based therapies.

Three-Dimensional Cell-Based Bioprinting for Soft Tissue Regeneration

김지현,James J. Yoo,이상진 한국조직공학과 재생의학회 2016 조직공학과 재생의학 Vol.13 No.6

Three-dimensional (3D) bioprinting technologies have been developed to offer construction of biological tissue constructs that mimic the anatomical and functional features of native tissues or organs. These cutting-edge technologies could make it possible to precisely place multiple cell types and biomaterials in a single 3D tissue construct. Hence, 3D bioprinting is one of the most attractive and powerful tools to provide more anatomical and functional similarity of human tissues or organs in tissue engineering and regenerative medicine. In recent years, this 3D bioprinting continually shows promise for building complex soft tissue constructs through placement of cell-laden hydrogelbased bioinks in a layer-by-layer fashion. This review will discuss bioprinting technologies and their applications in soft tissue regeneration.

Cell-Based Therapy for Urinary Incontinence

배재현,James J. Yoo 대한비뇨의학회 2010 Investigative and Clinical Urology Vol.51 No.1

Urinary incontinence has become a societal problem that affects millions of people worldwide. Although numerous therapeutic modalities are available, none has been shown to be entirely satisfactory. Consequently, cell-based approaches using regenerative medicine technology have emerged as a potential solution that would provide a means of correcting anatomical deficiencies and restoring normal function. As such, numerous cell-based investigations have been performed to develop systems that are focused on addressing clinical needs. While most of these attempts remain in the experimental stages, several clinical trials are being designed or are in progress. This article provides an overview of the cell-based approaches that utilize various cell sources to develop effective treatment modalities for urinary incontinence.

Three-Dimensional Printing in Tissue Engineering and Regenerative Medicine

조종수,James J. Yoo 한국조직공학과 재생의학회 2016 조직공학과 재생의학 Vol.13 No.6

Bioactive Compounds for the Treatment of Renal Disease

조강수,고인갑,James J. Yoo 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.9

Kidney diseases including acute kidney injury and chronic kidney disease are among the largest health issues worldwide. Dialysisand kidney transplantation can replace a significant portion of renal function, however these treatments still have limitations. Toovercome these shortcomings, a variety of innovative efforts have been introduced, including cell-based therapies. During thepast decades, advances have been made in the stem cell and developmental biology, and tissue engineering. As part of such efforts,studies on renal cell therapy and artificial kidney developments have been conducted, and multiple therapeutic interventionshave shown promise in the pre-clinical and clinical settings. More recently, therapeutic cell-secreting secretomes have emerged asa potential alternative to cell-based approaches. This approach involves the use of renotropic factors, such as growth factors andcytokines, that are produced by cells and these factors have shown effectiveness in facilitating kidney function recovery. This reviewfocuses on the renotropic functions of bioactive compounds that provide protective and regenerative effects for kidney tissuerepair, based on the available data in the literature.

Investigation of thermal degradation with extrusion-based dispensing modules for 3D bioprinting technology

Lee, Hyungseok,Yoo, James J,Kang, Hyun-Wook,Cho, Dong-Woo IOP Publishing 2016 Biofabrication Vol.8 No.1

Relationship between reactive oxygen species and water-soluble organic compounds: Time-resolved benzene carboxylic acids measurement in the coastal area during the KORUS-AQ campaign

Bae, Min-Suk,Schauer, James J.,Lee, Taehyoung,Jeong, Ju-Hee,Kim, Yoo-Keun,Ro, Chul-Un,Song, Sang-Keun,Shon, Zang-Ho Elsevier 2017 Environmental pollution Vol.231 No.1

<P><B>Abstract</B></P> <P>This study investigated the relationship between water-soluble organic compounds of ambient particulate matter (PM) and cellular redox activity collected from May 28 to June 20 of 2016 at the west coastal site in the Republic of Korea during the KORea-US Air Quality (KORUS-AQ) campaign. Automatic four-hour integrated samples operated at a flow rate of 92 L per minute for the analysis of organic carbon (OC), water-soluble organic carbon (WSOC), elemental carbon (EC), water-soluble ions (WSIs), and benzene carboxylic acids (BCAs) were collected on a 47 mm quartz fiber filter. The influence of atmospheric transport processes was assessed by the Weather Research and Forecasting (WRF) model. OC, EC, WSOC, and BCA were determined by SUNET carbon analyzer, total organic carbon (TOC) analyzer, and liquid chromatography-mass spectrometry mass spectrometry (LC-MSMS), respectively. Twenty-four-hour integrated samples were collected for reactive oxygen species (ROS) analysis using a fluorogenic cell-based method to investigate the main chemical classes of toxicity. The results illustrate that WSOC and specific water-soluble species are associated with the oxidative potential of particulate matter. Pairwise correlation scatterplots between the daily-averaged WSOC and ROS (r<SUP>2</SUP> of 0.81), and 135-BCA and ROS (r<SUP>2</SUP> of 0.84), indicate that secondary organic aerosol production was highly associated with ROS activity. In addition, X-ray spectral analysis together with secondary electron images (SEIs) of PM<SUB>2.5</SUB> particles collected during high ROS concentration events clearly indicate that water-soluble organic aerosols are major contributors to PM<SUB>2.5</SUB> mass. This study provides insight into the components of particulate matter that are drivers of the oxidative potential of atmospheric particulate matter and potential tracers for this activity.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Sensitive Analysis of Underivatized Benzene Carboxylic Acids using Time-resolved PM2.5 Sequence Sampling. </LI> <LI> Toxicity intensities between reactive oxygen species and water-soluble compounds. </LI> <LI> Good coefficients of determination between reactive oxygen species and carboxylic acids. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>